Neurotoxicity associated with chronic exposure to dichlorophenoxyacetic acid (2,4-D)–a simulation of environmental exposure in adult rats

Detalhes bibliográficos
Autor(a) principal: Ueda, Rose Meire R.
Data de Publicação: 2021
Outros Autores: de Souza, Verena M., Magalhães, Letícia R., Chagas, Pedro Henrique N., Veras, Allice S. C. [UNESP], Teixeira, Giovana R. [UNESP], Nai, Gisele A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1080/03601234.2021.1939622
http://hdl.handle.net/11449/207861
Resumo: The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) is the second most widely used herbicide in the world. The objective of this study was to evaluate the neurotoxic effects and the possible role of the dysregulation of apoptosis in the genesis of brain damage in chronic exposure to 2,4-D in rats. Eighty adult male rats were distributed into eight groups (n = 10) and exposed orally (contaminated feed) and via inhalation, with two groups exposed to distilled water (control) and six to 2,4-D in three distinct concentrations. They were exposed for 6 months. A neurobehavioral assessment was performed, and the brain was collected for histopathology and immunohistochemistry. The animals in the control groups showed greater motility in the open-field test and a greater number of entries in the elevated-plus-maze test than those exposed to 2,4-D (p < 0.05). Neuronal necrosis was more incident in animals exposed to 2,4-D (p < 0.05). There was a negative correlation between the expression of BAX and the measurement of the cerebral cortex thickness (r = −0.713; p = 0.047). Regardless of the route of exposure, 2,4-D led to a deficit in neurobehavioral tests and decreased thickness of the cerebral cortex associated with increased expression of the pro-apoptotic protein BAX.
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spelling Neurotoxicity associated with chronic exposure to dichlorophenoxyacetic acid (2,4-D)–a simulation of environmental exposure in adult ratsbehaviorcell cycleCentral nervous systemenvironmentpesticidesThe herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) is the second most widely used herbicide in the world. The objective of this study was to evaluate the neurotoxic effects and the possible role of the dysregulation of apoptosis in the genesis of brain damage in chronic exposure to 2,4-D in rats. Eighty adult male rats were distributed into eight groups (n = 10) and exposed orally (contaminated feed) and via inhalation, with two groups exposed to distilled water (control) and six to 2,4-D in three distinct concentrations. They were exposed for 6 months. A neurobehavioral assessment was performed, and the brain was collected for histopathology and immunohistochemistry. The animals in the control groups showed greater motility in the open-field test and a greater number of entries in the elevated-plus-maze test than those exposed to 2,4-D (p < 0.05). Neuronal necrosis was more incident in animals exposed to 2,4-D (p < 0.05). There was a negative correlation between the expression of BAX and the measurement of the cerebral cortex thickness (r = −0.713; p = 0.047). Regardless of the route of exposure, 2,4-D led to a deficit in neurobehavioral tests and decreased thickness of the cerebral cortex associated with increased expression of the pro-apoptotic protein BAX.Faculty of Psychology Universidade do Oeste Paulista (UNOESTE)Faculty of Medicine Universidade do Oeste Paulista (UNOESTE)Department of Physical Education Faculdade de Ciências e Tecnologia Universidade Estadual Paulista “Júlio de Mesquita Filho” - UNESPDepartment of Pathology and Graduate Program in Animal Science Universidade do Oeste Paulista (UNOESTE)Department of Physical Education Faculdade de Ciências e Tecnologia Universidade Estadual Paulista “Júlio de Mesquita Filho” - UNESPUniversidade do Oeste Paulista (UNOESTE)Universidade Estadual Paulista (Unesp)Ueda, Rose Meire R.de Souza, Verena M.Magalhães, Letícia R.Chagas, Pedro Henrique N.Veras, Allice S. C. [UNESP]Teixeira, Giovana R. [UNESP]Nai, Gisele A.2021-06-25T11:02:17Z2021-06-25T11:02:17Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1080/03601234.2021.1939622Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes.1532-41090360-1234http://hdl.handle.net/11449/20786110.1080/03601234.2021.19396222-s2.0-85107747863Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastesinfo:eu-repo/semantics/openAccess2024-06-18T17:43:12Zoai:repositorio.unesp.br:11449/207861Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:53:25.850428Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Neurotoxicity associated with chronic exposure to dichlorophenoxyacetic acid (2,4-D)–a simulation of environmental exposure in adult rats
title Neurotoxicity associated with chronic exposure to dichlorophenoxyacetic acid (2,4-D)–a simulation of environmental exposure in adult rats
spellingShingle Neurotoxicity associated with chronic exposure to dichlorophenoxyacetic acid (2,4-D)–a simulation of environmental exposure in adult rats
Ueda, Rose Meire R.
behavior
cell cycle
Central nervous system
environment
pesticides
title_short Neurotoxicity associated with chronic exposure to dichlorophenoxyacetic acid (2,4-D)–a simulation of environmental exposure in adult rats
title_full Neurotoxicity associated with chronic exposure to dichlorophenoxyacetic acid (2,4-D)–a simulation of environmental exposure in adult rats
title_fullStr Neurotoxicity associated with chronic exposure to dichlorophenoxyacetic acid (2,4-D)–a simulation of environmental exposure in adult rats
title_full_unstemmed Neurotoxicity associated with chronic exposure to dichlorophenoxyacetic acid (2,4-D)–a simulation of environmental exposure in adult rats
title_sort Neurotoxicity associated with chronic exposure to dichlorophenoxyacetic acid (2,4-D)–a simulation of environmental exposure in adult rats
author Ueda, Rose Meire R.
author_facet Ueda, Rose Meire R.
de Souza, Verena M.
Magalhães, Letícia R.
Chagas, Pedro Henrique N.
Veras, Allice S. C. [UNESP]
Teixeira, Giovana R. [UNESP]
Nai, Gisele A.
author_role author
author2 de Souza, Verena M.
Magalhães, Letícia R.
Chagas, Pedro Henrique N.
Veras, Allice S. C. [UNESP]
Teixeira, Giovana R. [UNESP]
Nai, Gisele A.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Oeste Paulista (UNOESTE)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Ueda, Rose Meire R.
de Souza, Verena M.
Magalhães, Letícia R.
Chagas, Pedro Henrique N.
Veras, Allice S. C. [UNESP]
Teixeira, Giovana R. [UNESP]
Nai, Gisele A.
dc.subject.por.fl_str_mv behavior
cell cycle
Central nervous system
environment
pesticides
topic behavior
cell cycle
Central nervous system
environment
pesticides
description The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) is the second most widely used herbicide in the world. The objective of this study was to evaluate the neurotoxic effects and the possible role of the dysregulation of apoptosis in the genesis of brain damage in chronic exposure to 2,4-D in rats. Eighty adult male rats were distributed into eight groups (n = 10) and exposed orally (contaminated feed) and via inhalation, with two groups exposed to distilled water (control) and six to 2,4-D in three distinct concentrations. They were exposed for 6 months. A neurobehavioral assessment was performed, and the brain was collected for histopathology and immunohistochemistry. The animals in the control groups showed greater motility in the open-field test and a greater number of entries in the elevated-plus-maze test than those exposed to 2,4-D (p < 0.05). Neuronal necrosis was more incident in animals exposed to 2,4-D (p < 0.05). There was a negative correlation between the expression of BAX and the measurement of the cerebral cortex thickness (r = −0.713; p = 0.047). Regardless of the route of exposure, 2,4-D led to a deficit in neurobehavioral tests and decreased thickness of the cerebral cortex associated with increased expression of the pro-apoptotic protein BAX.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T11:02:17Z
2021-06-25T11:02:17Z
2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1080/03601234.2021.1939622
Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes.
1532-4109
0360-1234
http://hdl.handle.net/11449/207861
10.1080/03601234.2021.1939622
2-s2.0-85107747863
url http://dx.doi.org/10.1080/03601234.2021.1939622
http://hdl.handle.net/11449/207861
identifier_str_mv Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes.
1532-4109
0360-1234
10.1080/03601234.2021.1939622
2-s2.0-85107747863
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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