Efeitos da estabulação sobre o trato respiratório de equinos em dois tipos diferentes de camas e a pasto

• Autor(a) principal: Calciolari, Karina [UNESP]
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/138024
• topic: Bronchoalveolar lavage; Wood shavings; Sawdust; Allergic process; Fungi; Horses; Lavado broncoalveolar; Maravalha; Serragem; Processo alérgico; Fungos; Cavalos

Horse respiratory diseases is second most commom cause of poor performance and premature retirement, which the one are musculoskeletal disorders. The stabling and poor quality feedings may the favor predispose of diseases in airways. In order we measure the influence of the types of bedding used in the horse stalls about responses allergic airway. Three groups of five horses each are formated, two groups kept in stables but each group with a different type of bedding (autoclaved wood- MA shaving and sawdust-SE) . The third group is the control and stays at pasture. The groups remained in this condition for 45 days with biweekly reviews (D0, D15, D30 and D45). It was realize the physical examination, venous and arterial blood samples collection to realize hemogram and hemogasometry analysis, the bronchoalveolar lavage samples was colected to realize the cytological analysis and to measure alkaline phosphatase. In addition, samples of the beds (clean and dirty) and haylage was collected to realize mycological analysis. In the physical examination was noticed higher intensity of coughing and sneezing at SE group. Hematological analysis remained within the normal standards. Findings of hemogasometric analysis were observed the biggest pH values at horses to pasture (7.47 ± 0.02). At stables horses was observed smaller values of partial pressure dioxide (D15 to D45), (values between 38.3 to 43.7 mmHg) to horses to pasture (values between 43.7 to 46.5 mmHg). The values oxygen partial pressure it was inversely proportional to partial pressure dioxide (MA and SE: values between 103.6 to 127.78 mmHg and pasture: 88.2 to 103.6 mmHg). It was observate higher values of nucleated cells of bronchoalveolar lavage, at all times, in animals to pasture (251.0 to 336.8 x 103 / mL), followed by the SE group (197.0 to 248.6 x 103 / mL) and finally MA group (140.6 to 270.2 x 103 / mL). The macrophages cells represented the biggest percentage of nucleates cells on bronchoalveolar lavage followed by lymphocytes (51.2 to 80.8% and 6.6 to 33.2% respectively). An unexpected pattern was observed in percentange of neutrophils cells, that it was a decrease over the time. One exception it was with MA group that had the biggest percentage values in D30 (35%), it was related to alkaline phosphatase peak at same time (27.6 ± 14.98 IU / L). Were identified about 12 fungi genres in both beds and 17 fungi genres at haylage. We indentified pathogenic genres in haylage as Aspergillus (0.85 ± 2.2%), Fusarium (5.35 ± 9.23%) and Penicillium (4 , 81 ± 9.53%), however in lower percentages. It was observate a difference production capacity and fine particle dispersion between the bed, that the biggest it was notice at sawdust (MA: 15.29% and SE: 18.28%). It was concluded that wood shavings bed is easier to use and showed less irritative capacity upper airway to que sawdust. As management and stabling conditions used were ideals and it was a ideal model of stables.