Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin A4
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2016/2457532 http://hdl.handle.net/11449/172958 |
Resumo: | We investigated the effects of Crotoxin (CTX), the main toxin of South American rattlesnake (Crotalus durissus terrificus) venom, on Walker 256 tumor growth, the pain symptoms associated (hyperalgesia and allodynia), and participation of endogenous lipoxin A4. Treatment with CTX (s.c.), daily, for 5 days reduced tumor growth at the 5th day after injection of Walker 256 carcinoma cells into the plantar surface of adult rat hind paw. This observation was associated with inhibition of new blood vessel formation and decrease in blood vessel diameter. The treatment with CTX raised plasma concentrations of lipoxin A4 and its natural analogue 15-epi-LXA4, an effect mediated by formyl peptide receptors (FPRs). In fact, the treatment with Boc-2, an inhibitor of FPRs, abolished the increase in plasma levels of these mediators triggered by CTX. The blockage of these receptors also abolished the inhibitory action of CTX on tumor growth and blood vessel formation and the decrease in blood vessel diameter. Together, the results herein presented demonstrate that CTX increases plasma concentrations of lipoxin A4 and 15-epi-LXA4, which might inhibit both tumor growth and formation of new vessels via FPRs. |
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Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin A4We investigated the effects of Crotoxin (CTX), the main toxin of South American rattlesnake (Crotalus durissus terrificus) venom, on Walker 256 tumor growth, the pain symptoms associated (hyperalgesia and allodynia), and participation of endogenous lipoxin A4. Treatment with CTX (s.c.), daily, for 5 days reduced tumor growth at the 5th day after injection of Walker 256 carcinoma cells into the plantar surface of adult rat hind paw. This observation was associated with inhibition of new blood vessel formation and decrease in blood vessel diameter. The treatment with CTX raised plasma concentrations of lipoxin A4 and its natural analogue 15-epi-LXA4, an effect mediated by formyl peptide receptors (FPRs). In fact, the treatment with Boc-2, an inhibitor of FPRs, abolished the increase in plasma levels of these mediators triggered by CTX. The blockage of these receptors also abolished the inhibitory action of CTX on tumor growth and blood vessel formation and the decrease in blood vessel diameter. Together, the results herein presented demonstrate that CTX increases plasma concentrations of lipoxin A4 and 15-epi-LXA4, which might inhibit both tumor growth and formation of new vessels via FPRs.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Special Laboratory of Pain and Signaling Butantan Institute, Avenida Vital Brazil 1500CEIS Department of Biology Institute of Biosciences of Rio Claro São Paulo State University (UNESP)Laboratory of Pathophysiology Butantan Institute, Avenida Vital Brazil 1500Department of Natural Sciences Mathematics and Education Agricultural Sciences Center Federal University of São Carlos, Rodovia Anhanguera Km 174Laboratory of Inflammation and Vascular Pharmacology Federal University of São Paulo, Rua São Nicolau 210Department of Physiology and Biophysics Institute of Biomedical Sciences University of São Paulo, Avenida Professor Lineu Prestes 1524Department of Pharmacology Institute of Biomedical Sciences University of São Paulo, Avenida Professor Lineu Prestes 1524CEIS Department of Biology Institute of Biosciences of Rio Claro São Paulo State University (UNESP)FAPESP: 07/52447-8Butantan InstituteUniversidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Universidade de São Paulo (USP)Brigatte, Patrícia [UNESP]Faiad, Odair JorgeFerreira Nocelli, Roberta CornélioLandgraf, Richardt G.Palma, Mario Sergio [UNESP]Cury, YaraCuri, RuiSampaio, Sandra Coccuzzo2018-12-11T17:02:53Z2018-12-11T17:02:53Z2016-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2016/2457532Mediators of Inflammation, v. 2016.1466-18610962-9351http://hdl.handle.net/11449/17295810.1155/2016/24575322-s2.0-849691949622-s2.0-84969194962.pdf2901888624506535Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMediators of Inflammation1,3701,370info:eu-repo/semantics/openAccess2023-12-05T06:16:05Zoai:repositorio.unesp.br:11449/172958Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:32:03.467497Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin A4 |
title |
Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin A4 |
spellingShingle |
Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin A4 Brigatte, Patrícia [UNESP] |
title_short |
Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin A4 |
title_full |
Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin A4 |
title_fullStr |
Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin A4 |
title_full_unstemmed |
Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin A4 |
title_sort |
Walker 256 tumor growth suppression by crotoxin involves formyl peptide receptors and lipoxin A4 |
author |
Brigatte, Patrícia [UNESP] |
author_facet |
Brigatte, Patrícia [UNESP] Faiad, Odair Jorge Ferreira Nocelli, Roberta Cornélio Landgraf, Richardt G. Palma, Mario Sergio [UNESP] Cury, Yara Curi, Rui Sampaio, Sandra Coccuzzo |
author_role |
author |
author2 |
Faiad, Odair Jorge Ferreira Nocelli, Roberta Cornélio Landgraf, Richardt G. Palma, Mario Sergio [UNESP] Cury, Yara Curi, Rui Sampaio, Sandra Coccuzzo |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Butantan Institute Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Brigatte, Patrícia [UNESP] Faiad, Odair Jorge Ferreira Nocelli, Roberta Cornélio Landgraf, Richardt G. Palma, Mario Sergio [UNESP] Cury, Yara Curi, Rui Sampaio, Sandra Coccuzzo |
description |
We investigated the effects of Crotoxin (CTX), the main toxin of South American rattlesnake (Crotalus durissus terrificus) venom, on Walker 256 tumor growth, the pain symptoms associated (hyperalgesia and allodynia), and participation of endogenous lipoxin A4. Treatment with CTX (s.c.), daily, for 5 days reduced tumor growth at the 5th day after injection of Walker 256 carcinoma cells into the plantar surface of adult rat hind paw. This observation was associated with inhibition of new blood vessel formation and decrease in blood vessel diameter. The treatment with CTX raised plasma concentrations of lipoxin A4 and its natural analogue 15-epi-LXA4, an effect mediated by formyl peptide receptors (FPRs). In fact, the treatment with Boc-2, an inhibitor of FPRs, abolished the increase in plasma levels of these mediators triggered by CTX. The blockage of these receptors also abolished the inhibitory action of CTX on tumor growth and blood vessel formation and the decrease in blood vessel diameter. Together, the results herein presented demonstrate that CTX increases plasma concentrations of lipoxin A4 and 15-epi-LXA4, which might inhibit both tumor growth and formation of new vessels via FPRs. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-01-01 2018-12-11T17:02:53Z 2018-12-11T17:02:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2016/2457532 Mediators of Inflammation, v. 2016. 1466-1861 0962-9351 http://hdl.handle.net/11449/172958 10.1155/2016/2457532 2-s2.0-84969194962 2-s2.0-84969194962.pdf 2901888624506535 |
url |
http://dx.doi.org/10.1155/2016/2457532 http://hdl.handle.net/11449/172958 |
identifier_str_mv |
Mediators of Inflammation, v. 2016. 1466-1861 0962-9351 10.1155/2016/2457532 2-s2.0-84969194962 2-s2.0-84969194962.pdf 2901888624506535 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mediators of Inflammation 1,370 1,370 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129082320224256 |