Cytogenetic evidence for de novo synthesis of rRNA and involvement of nucleolar material in the organization of cell structures during spermiogenesis of Chariesterus armatus (Heteroptera, Coreidae).
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4238/vol9-3gmr930 http://hdl.handle.net/11449/72108 |
Resumo: | The nucleolar material of Chariesterus armatus was analyzed during spermiogenesis in cell preparations impregnated with silver nitrate. Nucleolar corpuscles were observed in spermatids at the beginning of the process, showing that this organoid is also maintained after meiosis. In addition, nucleoli were seen in the round spermatids connected to the X-chromosome (bearer of the nucleolar organizer in C. armatus), indicating de novo synthesis of nucleolar material. This differs from the reorganization of ribosomal granules, transported from meiotic spermatocytes to round spermatids, where they would support protein synthesis, which is reported for other species. We also observed connections of nucleolar corpuscles to the nuclear membrane regions where the tail and the acrosome will be formed, suggesting close involvement of the nucleolar material in the formation of these structures. In addition to the nucleolar bodies, we detected silver-positive structures, which will require new approaches to clarify their role. One of these structures, observed in the cytoplasm, appears to correspond to the chromatoid body, which has been found in several organisms, but is still poorly understood; another is a complex structure to which the tail appears to be connected. We conclude that C. armatus is an appropriate model for understanding not only the synthesis of rRNA in the spermiogenesis, but also the functional meaning of the close relationship of nucleolar material with other structures during this process. |
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Cytogenetic evidence for de novo synthesis of rRNA and involvement of nucleolar material in the organization of cell structures during spermiogenesis of Chariesterus armatus (Heteroptera, Coreidae).ribosome RNAacrosomeanimalbiosynthesiscytogeneticscytologyHeteropteramalemetabolismmethodologynucleolusphysiologyspermatidspermatogenesisAcrosomeAnimalsCell NucleolusCytogeneticsMaleRNA, RibosomalSpermatidsSpermatogenesisThe nucleolar material of Chariesterus armatus was analyzed during spermiogenesis in cell preparations impregnated with silver nitrate. Nucleolar corpuscles were observed in spermatids at the beginning of the process, showing that this organoid is also maintained after meiosis. In addition, nucleoli were seen in the round spermatids connected to the X-chromosome (bearer of the nucleolar organizer in C. armatus), indicating de novo synthesis of nucleolar material. This differs from the reorganization of ribosomal granules, transported from meiotic spermatocytes to round spermatids, where they would support protein synthesis, which is reported for other species. We also observed connections of nucleolar corpuscles to the nuclear membrane regions where the tail and the acrosome will be formed, suggesting close involvement of the nucleolar material in the formation of these structures. In addition to the nucleolar bodies, we detected silver-positive structures, which will require new approaches to clarify their role. One of these structures, observed in the cytoplasm, appears to correspond to the chromatoid body, which has been found in several organisms, but is still poorly understood; another is a complex structure to which the tail appears to be connected. We conclude that C. armatus is an appropriate model for understanding not only the synthesis of rRNA in the spermiogenesis, but also the functional meaning of the close relationship of nucleolar material with other structures during this process.Universidade Estadual Paulista (Unesp)Arakaki, R. L.Souza, H. V.Castanhole, M. M.Bicudo, H. E.Itoyama, M. M.2014-05-27T11:25:22Z2014-05-27T11:25:22Z2010-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1877-1885application/pdfhttp://dx.doi.org/10.4238/vol9-3gmr930Genetics and molecular research : GMR, v. 9, n. 3, p. 1877-1885, 2010.1676-5680http://hdl.handle.net/11449/7210810.4238/vol9-3gmr9302-s2.0-799520606052-s2.0-79952060605.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and molecular research : GMR0,439info:eu-repo/semantics/openAccess2023-12-01T06:16:20Zoai:repositorio.unesp.br:11449/72108Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-01T06:16:20Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cytogenetic evidence for de novo synthesis of rRNA and involvement of nucleolar material in the organization of cell structures during spermiogenesis of Chariesterus armatus (Heteroptera, Coreidae). |
title |
Cytogenetic evidence for de novo synthesis of rRNA and involvement of nucleolar material in the organization of cell structures during spermiogenesis of Chariesterus armatus (Heteroptera, Coreidae). |
spellingShingle |
Cytogenetic evidence for de novo synthesis of rRNA and involvement of nucleolar material in the organization of cell structures during spermiogenesis of Chariesterus armatus (Heteroptera, Coreidae). Arakaki, R. L. ribosome RNA acrosome animal biosynthesis cytogenetics cytology Heteroptera male metabolism methodology nucleolus physiology spermatid spermatogenesis Acrosome Animals Cell Nucleolus Cytogenetics Male RNA, Ribosomal Spermatids Spermatogenesis |
title_short |
Cytogenetic evidence for de novo synthesis of rRNA and involvement of nucleolar material in the organization of cell structures during spermiogenesis of Chariesterus armatus (Heteroptera, Coreidae). |
title_full |
Cytogenetic evidence for de novo synthesis of rRNA and involvement of nucleolar material in the organization of cell structures during spermiogenesis of Chariesterus armatus (Heteroptera, Coreidae). |
title_fullStr |
Cytogenetic evidence for de novo synthesis of rRNA and involvement of nucleolar material in the organization of cell structures during spermiogenesis of Chariesterus armatus (Heteroptera, Coreidae). |
title_full_unstemmed |
Cytogenetic evidence for de novo synthesis of rRNA and involvement of nucleolar material in the organization of cell structures during spermiogenesis of Chariesterus armatus (Heteroptera, Coreidae). |
title_sort |
Cytogenetic evidence for de novo synthesis of rRNA and involvement of nucleolar material in the organization of cell structures during spermiogenesis of Chariesterus armatus (Heteroptera, Coreidae). |
author |
Arakaki, R. L. |
author_facet |
Arakaki, R. L. Souza, H. V. Castanhole, M. M. Bicudo, H. E. Itoyama, M. M. |
author_role |
author |
author2 |
Souza, H. V. Castanhole, M. M. Bicudo, H. E. Itoyama, M. M. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Arakaki, R. L. Souza, H. V. Castanhole, M. M. Bicudo, H. E. Itoyama, M. M. |
dc.subject.por.fl_str_mv |
ribosome RNA acrosome animal biosynthesis cytogenetics cytology Heteroptera male metabolism methodology nucleolus physiology spermatid spermatogenesis Acrosome Animals Cell Nucleolus Cytogenetics Male RNA, Ribosomal Spermatids Spermatogenesis |
topic |
ribosome RNA acrosome animal biosynthesis cytogenetics cytology Heteroptera male metabolism methodology nucleolus physiology spermatid spermatogenesis Acrosome Animals Cell Nucleolus Cytogenetics Male RNA, Ribosomal Spermatids Spermatogenesis |
description |
The nucleolar material of Chariesterus armatus was analyzed during spermiogenesis in cell preparations impregnated with silver nitrate. Nucleolar corpuscles were observed in spermatids at the beginning of the process, showing that this organoid is also maintained after meiosis. In addition, nucleoli were seen in the round spermatids connected to the X-chromosome (bearer of the nucleolar organizer in C. armatus), indicating de novo synthesis of nucleolar material. This differs from the reorganization of ribosomal granules, transported from meiotic spermatocytes to round spermatids, where they would support protein synthesis, which is reported for other species. We also observed connections of nucleolar corpuscles to the nuclear membrane regions where the tail and the acrosome will be formed, suggesting close involvement of the nucleolar material in the formation of these structures. In addition to the nucleolar bodies, we detected silver-positive structures, which will require new approaches to clarify their role. One of these structures, observed in the cytoplasm, appears to correspond to the chromatoid body, which has been found in several organisms, but is still poorly understood; another is a complex structure to which the tail appears to be connected. We conclude that C. armatus is an appropriate model for understanding not only the synthesis of rRNA in the spermiogenesis, but also the functional meaning of the close relationship of nucleolar material with other structures during this process. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-12-01 2014-05-27T11:25:22Z 2014-05-27T11:25:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4238/vol9-3gmr930 Genetics and molecular research : GMR, v. 9, n. 3, p. 1877-1885, 2010. 1676-5680 http://hdl.handle.net/11449/72108 10.4238/vol9-3gmr930 2-s2.0-79952060605 2-s2.0-79952060605.pdf |
url |
http://dx.doi.org/10.4238/vol9-3gmr930 http://hdl.handle.net/11449/72108 |
identifier_str_mv |
Genetics and molecular research : GMR, v. 9, n. 3, p. 1877-1885, 2010. 1676-5680 10.4238/vol9-3gmr930 2-s2.0-79952060605 2-s2.0-79952060605.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and molecular research : GMR 0,439 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1877-1885 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965133971128320 |