Interplay between 5-HT2C and 5-HT1A receptors in the dorsal periaqueductal gray in the modulation of fear-induced antinociception in mice

Detalhes bibliográficos
Autor(a) principal: Baptista-de-Souza, Daniela [UNESP]
Data de Publicação: 2018
Outros Autores: Pelarin, Vinícius [UNESP], Canto-de-Souza, Lucas [UNESP], Nunes-de-Souza, Ricardo Luiz [UNESP], Canto-de-Souza, Azair [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.neuropharm.2018.07.027
http://hdl.handle.net/11449/180167
Resumo: The confinement of rodents to the open arm of the elevated-plus maze provokes antinociception (OAA). As a type of defensive reaction, the OAA has been investigated through systemic and intramesencephalic (e.g., dorsal portion of the periaqueductal gray – dPAG) injections of anxiolytic-like drugs [e.g., serotonergic (5-HT) receptor agonists or antagonists]. Here we investigated the effects of (i) intra-dPAG injections of a 5HT2C receptor agonist (MK-212; 0.21 or 0.63 nmol) and antagonist (SB 242084; 0.01, 0.1 or 1.0 nmol); (ii) combined injections of SB 242084 and MK-212 into the dPAG; (iii) combined injections of SB 242084 with 8-OHDPAT (10 nmol) into the dPAG on the OAA in male Swiss mice. Nociception was assessed with the writhing test induced by acetic acid injection. Results showed that (i) intra-dPAG injection of MK-212 (0.63 nmol) increased the OAA; (ii) intra-dPAG SB 242084 (1.0 nmol) prevented the OAA; (iii) SB 242084 (0.1 nmol, a dose devoid of intrinsic effect on nociception) blocked the OAA enhancement provoked by MK-212 and enabled 8-OH-DPAT to prevent the OAA. These results suggest that OAA is mediated by 5-HT2C receptors within the dPAG. Intra-dPAG SB242084 administration provoked similar results on the effects produced by MK-212 and 8-OH-DPAT on OAA. In addition, the dPAG 5-HT1A and 5-HT2C receptors interact each other in the modulation of OAA.
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spelling Interplay between 5-HT2C and 5-HT1A receptors in the dorsal periaqueductal gray in the modulation of fear-induced antinociception in mice5-HT1A and 5HT2CAntinociceptionMicePeriaqueductal gray matterSerotoninThe confinement of rodents to the open arm of the elevated-plus maze provokes antinociception (OAA). As a type of defensive reaction, the OAA has been investigated through systemic and intramesencephalic (e.g., dorsal portion of the periaqueductal gray – dPAG) injections of anxiolytic-like drugs [e.g., serotonergic (5-HT) receptor agonists or antagonists]. Here we investigated the effects of (i) intra-dPAG injections of a 5HT2C receptor agonist (MK-212; 0.21 or 0.63 nmol) and antagonist (SB 242084; 0.01, 0.1 or 1.0 nmol); (ii) combined injections of SB 242084 and MK-212 into the dPAG; (iii) combined injections of SB 242084 with 8-OHDPAT (10 nmol) into the dPAG on the OAA in male Swiss mice. Nociception was assessed with the writhing test induced by acetic acid injection. Results showed that (i) intra-dPAG injection of MK-212 (0.63 nmol) increased the OAA; (ii) intra-dPAG SB 242084 (1.0 nmol) prevented the OAA; (iii) SB 242084 (0.1 nmol, a dose devoid of intrinsic effect on nociception) blocked the OAA enhancement provoked by MK-212 and enabled 8-OH-DPAT to prevent the OAA. These results suggest that OAA is mediated by 5-HT2C receptors within the dPAG. Intra-dPAG SB242084 administration provoked similar results on the effects produced by MK-212 and 8-OH-DPAT on OAA. In addition, the dPAG 5-HT1A and 5-HT2C receptors interact each other in the modulation of OAA.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Dept. Psychology Federal University of São Carlos-UFSCarLab. Pharmacology School of Pharmaceutical Sciences Univ. Estadual Paulista – UNESPJoint Graduate Program in Physiological Sciences UFSCar/UNESPGraduate Program in Psychology UFSCar, Rod. Washington Luís, Km 235Institute of Neuroscience and Behavior, Av. Do Café 2.450Lab. Pharmacology School of Pharmaceutical Sciences Univ. Estadual Paulista – UNESPJoint Graduate Program in Physiological Sciences UFSCar/UNESPFAPESP: 2009/17938-6FAPESP: 2010/06654-4FAPESP: 2011/19472-4CNPq: 306556/2015-4CNPq: 309201/2015-2CNPq: 482356/2013-8Universidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (Unesp)Institute of Neuroscience and BehaviorBaptista-de-Souza, Daniela [UNESP]Pelarin, Vinícius [UNESP]Canto-de-Souza, Lucas [UNESP]Nunes-de-Souza, Ricardo Luiz [UNESP]Canto-de-Souza, Azair [UNESP]2018-12-11T17:38:26Z2018-12-11T17:38:26Z2018-09-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article100-106application/pdfhttp://dx.doi.org/10.1016/j.neuropharm.2018.07.027Neuropharmacology, v. 140, p. 100-106.1873-70640028-3908http://hdl.handle.net/11449/18016710.1016/j.neuropharm.2018.07.0272-s2.0-850530450212-s2.0-85053045021.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNeuropharmacology2,043info:eu-repo/semantics/openAccess2024-06-24T14:51:25Zoai:repositorio.unesp.br:11449/180167Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:29:55.416824Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Interplay between 5-HT2C and 5-HT1A receptors in the dorsal periaqueductal gray in the modulation of fear-induced antinociception in mice
title Interplay between 5-HT2C and 5-HT1A receptors in the dorsal periaqueductal gray in the modulation of fear-induced antinociception in mice
spellingShingle Interplay between 5-HT2C and 5-HT1A receptors in the dorsal periaqueductal gray in the modulation of fear-induced antinociception in mice
Baptista-de-Souza, Daniela [UNESP]
5-HT1A and 5HT2C
Antinociception
Mice
Periaqueductal gray matter
Serotonin
title_short Interplay between 5-HT2C and 5-HT1A receptors in the dorsal periaqueductal gray in the modulation of fear-induced antinociception in mice
title_full Interplay between 5-HT2C and 5-HT1A receptors in the dorsal periaqueductal gray in the modulation of fear-induced antinociception in mice
title_fullStr Interplay between 5-HT2C and 5-HT1A receptors in the dorsal periaqueductal gray in the modulation of fear-induced antinociception in mice
title_full_unstemmed Interplay between 5-HT2C and 5-HT1A receptors in the dorsal periaqueductal gray in the modulation of fear-induced antinociception in mice
title_sort Interplay between 5-HT2C and 5-HT1A receptors in the dorsal periaqueductal gray in the modulation of fear-induced antinociception in mice
author Baptista-de-Souza, Daniela [UNESP]
author_facet Baptista-de-Souza, Daniela [UNESP]
Pelarin, Vinícius [UNESP]
Canto-de-Souza, Lucas [UNESP]
Nunes-de-Souza, Ricardo Luiz [UNESP]
Canto-de-Souza, Azair [UNESP]
author_role author
author2 Pelarin, Vinícius [UNESP]
Canto-de-Souza, Lucas [UNESP]
Nunes-de-Souza, Ricardo Luiz [UNESP]
Canto-de-Souza, Azair [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de São Carlos (UFSCar)
Universidade Estadual Paulista (Unesp)
Institute of Neuroscience and Behavior
dc.contributor.author.fl_str_mv Baptista-de-Souza, Daniela [UNESP]
Pelarin, Vinícius [UNESP]
Canto-de-Souza, Lucas [UNESP]
Nunes-de-Souza, Ricardo Luiz [UNESP]
Canto-de-Souza, Azair [UNESP]
dc.subject.por.fl_str_mv 5-HT1A and 5HT2C
Antinociception
Mice
Periaqueductal gray matter
Serotonin
topic 5-HT1A and 5HT2C
Antinociception
Mice
Periaqueductal gray matter
Serotonin
description The confinement of rodents to the open arm of the elevated-plus maze provokes antinociception (OAA). As a type of defensive reaction, the OAA has been investigated through systemic and intramesencephalic (e.g., dorsal portion of the periaqueductal gray – dPAG) injections of anxiolytic-like drugs [e.g., serotonergic (5-HT) receptor agonists or antagonists]. Here we investigated the effects of (i) intra-dPAG injections of a 5HT2C receptor agonist (MK-212; 0.21 or 0.63 nmol) and antagonist (SB 242084; 0.01, 0.1 or 1.0 nmol); (ii) combined injections of SB 242084 and MK-212 into the dPAG; (iii) combined injections of SB 242084 with 8-OHDPAT (10 nmol) into the dPAG on the OAA in male Swiss mice. Nociception was assessed with the writhing test induced by acetic acid injection. Results showed that (i) intra-dPAG injection of MK-212 (0.63 nmol) increased the OAA; (ii) intra-dPAG SB 242084 (1.0 nmol) prevented the OAA; (iii) SB 242084 (0.1 nmol, a dose devoid of intrinsic effect on nociception) blocked the OAA enhancement provoked by MK-212 and enabled 8-OH-DPAT to prevent the OAA. These results suggest that OAA is mediated by 5-HT2C receptors within the dPAG. Intra-dPAG SB242084 administration provoked similar results on the effects produced by MK-212 and 8-OH-DPAT on OAA. In addition, the dPAG 5-HT1A and 5-HT2C receptors interact each other in the modulation of OAA.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:38:26Z
2018-12-11T17:38:26Z
2018-09-15
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.neuropharm.2018.07.027
Neuropharmacology, v. 140, p. 100-106.
1873-7064
0028-3908
http://hdl.handle.net/11449/180167
10.1016/j.neuropharm.2018.07.027
2-s2.0-85053045021
2-s2.0-85053045021.pdf
url http://dx.doi.org/10.1016/j.neuropharm.2018.07.027
http://hdl.handle.net/11449/180167
identifier_str_mv Neuropharmacology, v. 140, p. 100-106.
1873-7064
0028-3908
10.1016/j.neuropharm.2018.07.027
2-s2.0-85053045021
2-s2.0-85053045021.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Neuropharmacology
2,043
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 100-106
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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