Circulating Extracellular Vesicles microRNAs Are Altered in Women Undergoing Preterm Birth
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/ijms24065527 http://hdl.handle.net/11449/248703 |
Resumo: | Preterm labor (PTL) and preterm premature rupture of membranes (PPROM) lead to high perinatal morbidity/mortality rates worldwide. Small extracellular vesicles (sEV) act in cell communication and contain microRNAs that may contribute to the pathogenesis of these complications. We aimed to compare the expression, in sEV from peripheral blood, of miRNAs between term and preterm pregnancies. This cross-sectional study included women who underwent PTL, PPROM, and term pregnancies, examined at the Botucatu Medical School Hospital, SP, Brazil. sEV were isolated from plasma. Western blot used to detect exosomal protein CD63 and nanoparticle tracking analysis were performed. The expression of 800 miRNAs was assessed by the nCounter Humanv3 miRNA Assay (NanoString). The miRNA expression and relative risk were determined. Samples from 31 women—15 preterm and 16 term—were included. miR-612 expression was increased in the preterm groups. miR-612 has been shown to increase apoptosis in tumor cells and to regulate the nuclear factor κB inflammatory pathway, processes involved in PTL/PPROM pathogenesis. miR-1253, miR-1283, miR378e, and miR-579-3p, all associated with cellular senescence, were downregulated in PPROM compared with term pregnancies. We conclude that miRNAs from circulating sEV are differentially expressed between term and preterm pregnancies and modulate genes in pathways that are relevant to PTL/PPROM pathogenesis. |
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Circulating Extracellular Vesicles microRNAs Are Altered in Women Undergoing Preterm BirthinflammationmicroRNAspreterm birthpreterm laborpreterm premature rupture of membranessmall extracellular vesiclesPreterm labor (PTL) and preterm premature rupture of membranes (PPROM) lead to high perinatal morbidity/mortality rates worldwide. Small extracellular vesicles (sEV) act in cell communication and contain microRNAs that may contribute to the pathogenesis of these complications. We aimed to compare the expression, in sEV from peripheral blood, of miRNAs between term and preterm pregnancies. This cross-sectional study included women who underwent PTL, PPROM, and term pregnancies, examined at the Botucatu Medical School Hospital, SP, Brazil. sEV were isolated from plasma. Western blot used to detect exosomal protein CD63 and nanoparticle tracking analysis were performed. The expression of 800 miRNAs was assessed by the nCounter Humanv3 miRNA Assay (NanoString). The miRNA expression and relative risk were determined. Samples from 31 women—15 preterm and 16 term—were included. miR-612 expression was increased in the preterm groups. miR-612 has been shown to increase apoptosis in tumor cells and to regulate the nuclear factor κB inflammatory pathway, processes involved in PTL/PPROM pathogenesis. miR-1253, miR-1283, miR378e, and miR-579-3p, all associated with cellular senescence, were downregulated in PPROM compared with term pregnancies. We conclude that miRNAs from circulating sEV are differentially expressed between term and preterm pregnancies and modulate genes in pathways that are relevant to PTL/PPROM pathogenesis.Department of Pathology Botucatu Medical School São Paulo State University (UNESP), SPFaculty of Medicine—Jaú Campus University of Western São Paulo (UNOESTE), SPFaculty of Veterinary Medicine and Animal Science São Paulo State University (UNESP), SPExperimental Research Unity (UNIPEX) Botucatu Medical School São Paulo State University (UNESP), SPDepartment of Surgery and Orthopedics Botucatu Medical School São Paulo State University (UNESP), SPDepartment of Veterinary Medicine Faculty of Animal Science and Food Engineering São Paulo University (USP), SPDepartment of Pathology Botucatu Medical School São Paulo State University (UNESP), SPFaculty of Veterinary Medicine and Animal Science São Paulo State University (UNESP), SPExperimental Research Unity (UNIPEX) Botucatu Medical School São Paulo State University (UNESP), SPDepartment of Surgery and Orthopedics Botucatu Medical School São Paulo State University (UNESP), SPUniversidade Estadual Paulista (UNESP)University of Western São Paulo (UNOESTE)Universidade de São Paulo (USP)Ramos, Bruna Ribeiro Andrade [UNESP]Tronco, Júlia Abbade [UNESP]Carvalho, Márcio [UNESP]Felix, Tainara Francini [UNESP]Reis, Patrícia Pintor [UNESP]Silveira, Juliano CoelhoSilva, Márcia Guimarães [UNESP]2023-07-29T13:51:14Z2023-07-29T13:51:14Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/ijms24065527International Journal of Molecular Sciences, v. 24, n. 6, 2023.1422-00671661-6596http://hdl.handle.net/11449/24870310.3390/ijms240655272-s2.0-85152691951Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Molecular Sciencesinfo:eu-repo/semantics/openAccess2024-09-03T13:15:27Zoai:repositorio.unesp.br:11449/248703Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:15:27Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Circulating Extracellular Vesicles microRNAs Are Altered in Women Undergoing Preterm Birth |
title |
Circulating Extracellular Vesicles microRNAs Are Altered in Women Undergoing Preterm Birth |
spellingShingle |
Circulating Extracellular Vesicles microRNAs Are Altered in Women Undergoing Preterm Birth Ramos, Bruna Ribeiro Andrade [UNESP] inflammation microRNAs preterm birth preterm labor preterm premature rupture of membranes small extracellular vesicles |
title_short |
Circulating Extracellular Vesicles microRNAs Are Altered in Women Undergoing Preterm Birth |
title_full |
Circulating Extracellular Vesicles microRNAs Are Altered in Women Undergoing Preterm Birth |
title_fullStr |
Circulating Extracellular Vesicles microRNAs Are Altered in Women Undergoing Preterm Birth |
title_full_unstemmed |
Circulating Extracellular Vesicles microRNAs Are Altered in Women Undergoing Preterm Birth |
title_sort |
Circulating Extracellular Vesicles microRNAs Are Altered in Women Undergoing Preterm Birth |
author |
Ramos, Bruna Ribeiro Andrade [UNESP] |
author_facet |
Ramos, Bruna Ribeiro Andrade [UNESP] Tronco, Júlia Abbade [UNESP] Carvalho, Márcio [UNESP] Felix, Tainara Francini [UNESP] Reis, Patrícia Pintor [UNESP] Silveira, Juliano Coelho Silva, Márcia Guimarães [UNESP] |
author_role |
author |
author2 |
Tronco, Júlia Abbade [UNESP] Carvalho, Márcio [UNESP] Felix, Tainara Francini [UNESP] Reis, Patrícia Pintor [UNESP] Silveira, Juliano Coelho Silva, Márcia Guimarães [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Western São Paulo (UNOESTE) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Ramos, Bruna Ribeiro Andrade [UNESP] Tronco, Júlia Abbade [UNESP] Carvalho, Márcio [UNESP] Felix, Tainara Francini [UNESP] Reis, Patrícia Pintor [UNESP] Silveira, Juliano Coelho Silva, Márcia Guimarães [UNESP] |
dc.subject.por.fl_str_mv |
inflammation microRNAs preterm birth preterm labor preterm premature rupture of membranes small extracellular vesicles |
topic |
inflammation microRNAs preterm birth preterm labor preterm premature rupture of membranes small extracellular vesicles |
description |
Preterm labor (PTL) and preterm premature rupture of membranes (PPROM) lead to high perinatal morbidity/mortality rates worldwide. Small extracellular vesicles (sEV) act in cell communication and contain microRNAs that may contribute to the pathogenesis of these complications. We aimed to compare the expression, in sEV from peripheral blood, of miRNAs between term and preterm pregnancies. This cross-sectional study included women who underwent PTL, PPROM, and term pregnancies, examined at the Botucatu Medical School Hospital, SP, Brazil. sEV were isolated from plasma. Western blot used to detect exosomal protein CD63 and nanoparticle tracking analysis were performed. The expression of 800 miRNAs was assessed by the nCounter Humanv3 miRNA Assay (NanoString). The miRNA expression and relative risk were determined. Samples from 31 women—15 preterm and 16 term—were included. miR-612 expression was increased in the preterm groups. miR-612 has been shown to increase apoptosis in tumor cells and to regulate the nuclear factor κB inflammatory pathway, processes involved in PTL/PPROM pathogenesis. miR-1253, miR-1283, miR378e, and miR-579-3p, all associated with cellular senescence, were downregulated in PPROM compared with term pregnancies. We conclude that miRNAs from circulating sEV are differentially expressed between term and preterm pregnancies and modulate genes in pathways that are relevant to PTL/PPROM pathogenesis. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:51:14Z 2023-07-29T13:51:14Z 2023-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/ijms24065527 International Journal of Molecular Sciences, v. 24, n. 6, 2023. 1422-0067 1661-6596 http://hdl.handle.net/11449/248703 10.3390/ijms24065527 2-s2.0-85152691951 |
url |
http://dx.doi.org/10.3390/ijms24065527 http://hdl.handle.net/11449/248703 |
identifier_str_mv |
International Journal of Molecular Sciences, v. 24, n. 6, 2023. 1422-0067 1661-6596 10.3390/ijms24065527 2-s2.0-85152691951 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Molecular Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021391860236288 |