Temporal Analysis of Candidatus Liberibacter asiaticus in Citrandarin Genotypes Indicates Unstable Infection
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/agronomy12102566 http://hdl.handle.net/11449/245111 |
Resumo: | Background: Huanglongbing (HLB) is currently one of the most devasting diseases in citrus plants worldwide. Resistance against its causal agent, Candidatus Liberibacter asiaticus (CLas), in commercial Citrus species remains a challenge, even though they show differences in CLas multiplication. Methods: A total of 14 citrandarins and their parents (Sunki mandarin and Poncirus trifoliata cv. Rubidoux) were top-grafted onto the canopy of potted 'Valencia' sweet orange plants with high CLas titers. The grafted genotypes were tested for CLas infection and physiological effects of the disease (starch accumulation and callose deposition) for 12 months. Results: All tested genotypes were infected by CLas during the time frame of the experiment. However, a decrease in the infection rate ranging from 50% to 80% for the hybrids H109, H126, H157, and H222 was observed 360 days from the top-grafting. CLas was undetected by real-time PCR in H106 at the end of the experiment, which had low levels of starch and callose deposition. Conclusions: CLas infected all of the tested citrandarins, but a decrease in the infection rate over time was detected for some specific genotypes, which led to less starch accumulation and callose deposition. |
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Temporal Analysis of Candidatus Liberibacter asiaticus in Citrandarin Genotypes Indicates Unstable InfectionHuanglongbingcitrus rootstockdisease tolerancebacteriaBackground: Huanglongbing (HLB) is currently one of the most devasting diseases in citrus plants worldwide. Resistance against its causal agent, Candidatus Liberibacter asiaticus (CLas), in commercial Citrus species remains a challenge, even though they show differences in CLas multiplication. Methods: A total of 14 citrandarins and their parents (Sunki mandarin and Poncirus trifoliata cv. Rubidoux) were top-grafted onto the canopy of potted 'Valencia' sweet orange plants with high CLas titers. The grafted genotypes were tested for CLas infection and physiological effects of the disease (starch accumulation and callose deposition) for 12 months. Results: All tested genotypes were infected by CLas during the time frame of the experiment. However, a decrease in the infection rate ranging from 50% to 80% for the hybrids H109, H126, H157, and H222 was observed 360 days from the top-grafting. CLas was undetected by real-time PCR in H106 at the end of the experiment, which had low levels of starch and callose deposition. Conclusions: CLas infected all of the tested citrandarins, but a decrease in the infection rate over time was detected for some specific genotypes, which led to less starch accumulation and callose deposition.Fundação de Amparo de Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Inst Agron Campinas, Ctr APTA Citros Sylvio Moreira, BR-13492442 Cordeiropolis, SP, BrazilUniv Fed Sao Carlos, Dept Tecnol Agroind & Socioecon Rural, BR-13600970 Araras, SP, BrazilUNESP, FCAV, BR-14884900 Jaboticabal, SP, BrazilUNESP, FCAV, BR-14884900 Jaboticabal, SP, BrazilFAPESP: FAPESP-2020/07045-3FAPESP: FAPESP-2019/06412-5FAPESP: FAPESP-2021/03989-0CNPq: 308164/2021-0CNPq: 313295/2020-4MdpiInst Agron CampinasUniversidade Federal de São Carlos (UFSCar)Universidade Estadual Paulista (UNESP)Cavichioli, Thais MagniCurtolo, MaiaraCristofani-Yaly, MariangelaRodrigues, JosianeDella Coletta-Filho, Helvecio [UNESP]2023-07-29T11:37:33Z2023-07-29T11:37:33Z2022-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11http://dx.doi.org/10.3390/agronomy12102566Agronomy-basel. Basel: Mdpi, v. 12, n. 10, 11 p., 2022.http://hdl.handle.net/11449/24511110.3390/agronomy12102566WOS:000872034600001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAgronomy-baselinfo:eu-repo/semantics/openAccess2023-07-29T11:37:33Zoai:repositorio.unesp.br:11449/245111Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:35:48.575694Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Temporal Analysis of Candidatus Liberibacter asiaticus in Citrandarin Genotypes Indicates Unstable Infection |
title |
Temporal Analysis of Candidatus Liberibacter asiaticus in Citrandarin Genotypes Indicates Unstable Infection |
spellingShingle |
Temporal Analysis of Candidatus Liberibacter asiaticus in Citrandarin Genotypes Indicates Unstable Infection Cavichioli, Thais Magni Huanglongbing citrus rootstock disease tolerance bacteria |
title_short |
Temporal Analysis of Candidatus Liberibacter asiaticus in Citrandarin Genotypes Indicates Unstable Infection |
title_full |
Temporal Analysis of Candidatus Liberibacter asiaticus in Citrandarin Genotypes Indicates Unstable Infection |
title_fullStr |
Temporal Analysis of Candidatus Liberibacter asiaticus in Citrandarin Genotypes Indicates Unstable Infection |
title_full_unstemmed |
Temporal Analysis of Candidatus Liberibacter asiaticus in Citrandarin Genotypes Indicates Unstable Infection |
title_sort |
Temporal Analysis of Candidatus Liberibacter asiaticus in Citrandarin Genotypes Indicates Unstable Infection |
author |
Cavichioli, Thais Magni |
author_facet |
Cavichioli, Thais Magni Curtolo, Maiara Cristofani-Yaly, Mariangela Rodrigues, Josiane Della Coletta-Filho, Helvecio [UNESP] |
author_role |
author |
author2 |
Curtolo, Maiara Cristofani-Yaly, Mariangela Rodrigues, Josiane Della Coletta-Filho, Helvecio [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Inst Agron Campinas Universidade Federal de São Carlos (UFSCar) Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Cavichioli, Thais Magni Curtolo, Maiara Cristofani-Yaly, Mariangela Rodrigues, Josiane Della Coletta-Filho, Helvecio [UNESP] |
dc.subject.por.fl_str_mv |
Huanglongbing citrus rootstock disease tolerance bacteria |
topic |
Huanglongbing citrus rootstock disease tolerance bacteria |
description |
Background: Huanglongbing (HLB) is currently one of the most devasting diseases in citrus plants worldwide. Resistance against its causal agent, Candidatus Liberibacter asiaticus (CLas), in commercial Citrus species remains a challenge, even though they show differences in CLas multiplication. Methods: A total of 14 citrandarins and their parents (Sunki mandarin and Poncirus trifoliata cv. Rubidoux) were top-grafted onto the canopy of potted 'Valencia' sweet orange plants with high CLas titers. The grafted genotypes were tested for CLas infection and physiological effects of the disease (starch accumulation and callose deposition) for 12 months. Results: All tested genotypes were infected by CLas during the time frame of the experiment. However, a decrease in the infection rate ranging from 50% to 80% for the hybrids H109, H126, H157, and H222 was observed 360 days from the top-grafting. CLas was undetected by real-time PCR in H106 at the end of the experiment, which had low levels of starch and callose deposition. Conclusions: CLas infected all of the tested citrandarins, but a decrease in the infection rate over time was detected for some specific genotypes, which led to less starch accumulation and callose deposition. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-10-01 2023-07-29T11:37:33Z 2023-07-29T11:37:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/agronomy12102566 Agronomy-basel. Basel: Mdpi, v. 12, n. 10, 11 p., 2022. http://hdl.handle.net/11449/245111 10.3390/agronomy12102566 WOS:000872034600001 |
url |
http://dx.doi.org/10.3390/agronomy12102566 http://hdl.handle.net/11449/245111 |
identifier_str_mv |
Agronomy-basel. Basel: Mdpi, v. 12, n. 10, 11 p., 2022. 10.3390/agronomy12102566 WOS:000872034600001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Agronomy-basel |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 |
dc.publisher.none.fl_str_mv |
Mdpi |
publisher.none.fl_str_mv |
Mdpi |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129441799340032 |