Expressão dos filamentos intermediários no diagnóstico dos tumores mamários de cadelas

• Autor(a) principal: Zuccari, D. A. P. C.
• Data de Publicação: 2002
• Outros Autores: Santana, A. E. [UNESP], Rocha, N.S. [UNESP]
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/S0102-09352002000600005; http://hdl.handle.net/11449/67069
• topic: Dog; Immunohistochemistry; Mammary tumor; alpha actin; cytokeratin; monoclonal antibody; vimentin; breast tumor; controlled study; dog disease; female; histogenesis; immunohistochemistry; intermediate filament; malignant transformation; myoepithelium cell; nonhuman; protein expression; tumor classification; tumor diagnosis; Canis familiaris

Immunohistochemical evaluation was performed to study the histogenesis of canine mammary tumors and to contribute to a better understanding of their classification. Monoclonal antibodies specific for different types of intermediate filaments (cytokeratins, vimentin, α-actin) were used. Epithelial cells stained positively for cytokeratins and their expression was lost as the malignant transformation occurs. Myoepithelial cells stained positively for vimentin and α-actin. In contrast to vimentin, α-actin lost the expression as the cartilaginous or osseous metaplasia occurs. Immunohistochemical evaluation with monoclonal antibodies proved to be efficient for identification of tumor histogenesis.