Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDs

Detalhes bibliográficos
Autor(a) principal: de Lima, Lucas F.
Data de Publicação: 2022
Outros Autores: de Freitas, Amanda de S.M., Ferreira, André L., Maciel, Cristiane Carla [UNESP], Ferreira, Marystela, de Araujo, William R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.snr.2022.100102
http://hdl.handle.net/11449/241651
Resumo: There is an increasing demand for robust, affordable, and sensitive analytical devices for clinical diagnostics. Herein, we describe a simple route to produce biodegradable and disposable thermoplastic composite electrodes using the commercial polymer (Ecoflex®) and graphite microparticles. The surface of the carbon composite electrode was modified with gold nanoparticles conjugated with graphene quantum dots (Au@GQDs) for sensitive and non-enzymatic glucose sensing. The morphological and structural characterizations of the modified electrochemical device revealed a hydrophilic rough composite surface, with high dispersion of graphite in the polymer matrix and confirmed the successful superficial modification of the working electrode with spherical Au@GQDs nanoparticles. Cyclic voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) were used to electrochemically characterize the modified and nonmodified thermoplastic sensor, demonstrating a 4.5-fold enhancement of the electroactive area, higher heterogeneous electron transfer kinetics, and electrocatalytic properties for glucose sensing when the sensor was modified with Au@GQDs. The electrochemical determination of the glucose was carried out using an amperometric method applying -0.15 V in alkaline conditions (0.1 mol L−1 NaOH). Under optimized conditions, the proposed method presented a high sensitivity (4.85 A L mol−1) and a low limit of detection (LOD) of 9.12 µmol L−1. The Ecoflex:graphite/Au@GQDs sensor was applied for glucose sensing in synthetic saliva samples with recovery values ranging from 97.84% to 105.20%, highlighting the excellent accuracy of the proposed method. Finally, the electrode was subjected to thermal and biological degradation studies and presented disposable and eco-friendly characteristics. Based on these results, we believe that this new thermoplastic composite sensor modified with Au@GQDs can be an excellent candidate for a disposable, low-cost, and enzymeless electrochemical glucose sensor.
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spelling Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDsBiodegradable materialEnzyme-free sensorGlucose detectionLow-cost electrochemical sensorThermoplastic composite electrodesThere is an increasing demand for robust, affordable, and sensitive analytical devices for clinical diagnostics. Herein, we describe a simple route to produce biodegradable and disposable thermoplastic composite electrodes using the commercial polymer (Ecoflex®) and graphite microparticles. The surface of the carbon composite electrode was modified with gold nanoparticles conjugated with graphene quantum dots (Au@GQDs) for sensitive and non-enzymatic glucose sensing. The morphological and structural characterizations of the modified electrochemical device revealed a hydrophilic rough composite surface, with high dispersion of graphite in the polymer matrix and confirmed the successful superficial modification of the working electrode with spherical Au@GQDs nanoparticles. Cyclic voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) were used to electrochemically characterize the modified and nonmodified thermoplastic sensor, demonstrating a 4.5-fold enhancement of the electroactive area, higher heterogeneous electron transfer kinetics, and electrocatalytic properties for glucose sensing when the sensor was modified with Au@GQDs. The electrochemical determination of the glucose was carried out using an amperometric method applying -0.15 V in alkaline conditions (0.1 mol L−1 NaOH). Under optimized conditions, the proposed method presented a high sensitivity (4.85 A L mol−1) and a low limit of detection (LOD) of 9.12 µmol L−1. The Ecoflex:graphite/Au@GQDs sensor was applied for glucose sensing in synthetic saliva samples with recovery values ranging from 97.84% to 105.20%, highlighting the excellent accuracy of the proposed method. Finally, the electrode was subjected to thermal and biological degradation studies and presented disposable and eco-friendly characteristics. Based on these results, we believe that this new thermoplastic composite sensor modified with Au@GQDs can be an excellent candidate for a disposable, low-cost, and enzymeless electrochemical glucose sensor.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)American Sleep Medicine FoundationCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Portable Chemical Sensors Lab Department of Analytical Chemistry Institute of Chemistry State University of Campinas – UNICAMP, P.O. Box 6154, São PauloPolymers and Biopolymers Technology Lab. (TecPBio) Institute of Science and Technology (ICT) Federal University of São Paulo (UNIFESP), SPInstitute of Science and Technology São Paulo State University (UNESP)Center of Science and Technology for Sustainability (CCTS) Federal University of São Carlos (UFSCar), SPInstitute of Science and Technology São Paulo State University (UNESP)FAPESP: 2016/19387FAPESP: 2018/04061-8FAPESP: 2018/08782-1FAPESP: 2019/02692-3American Sleep Medicine Foundation: 88882.430936/2019-01CAPES: 88887.497915/2020-00Universidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Universidade Federal de São Carlos (UFSCar)de Lima, Lucas F.de Freitas, Amanda de S.M.Ferreira, André L.Maciel, Cristiane Carla [UNESP]Ferreira, Marystelade Araujo, William R.2023-03-01T21:14:56Z2023-03-01T21:14:56Z2022-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.snr.2022.100102Sensors and Actuators Reports, v. 4.2666-0539http://hdl.handle.net/11449/24165110.1016/j.snr.2022.1001022-s2.0-85129615875Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSensors and Actuators Reportsinfo:eu-repo/semantics/openAccess2023-03-01T21:14:57Zoai:repositorio.unesp.br:11449/241651Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T21:14:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDs
title Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDs
spellingShingle Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDs
de Lima, Lucas F.
Biodegradable material
Enzyme-free sensor
Glucose detection
Low-cost electrochemical sensor
Thermoplastic composite electrodes
title_short Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDs
title_full Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDs
title_fullStr Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDs
title_full_unstemmed Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDs
title_sort Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDs
author de Lima, Lucas F.
author_facet de Lima, Lucas F.
de Freitas, Amanda de S.M.
Ferreira, André L.
Maciel, Cristiane Carla [UNESP]
Ferreira, Marystela
de Araujo, William R.
author_role author
author2 de Freitas, Amanda de S.M.
Ferreira, André L.
Maciel, Cristiane Carla [UNESP]
Ferreira, Marystela
de Araujo, William R.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
Universidade Estadual Paulista (UNESP)
Universidade Federal de São Carlos (UFSCar)
dc.contributor.author.fl_str_mv de Lima, Lucas F.
de Freitas, Amanda de S.M.
Ferreira, André L.
Maciel, Cristiane Carla [UNESP]
Ferreira, Marystela
de Araujo, William R.
dc.subject.por.fl_str_mv Biodegradable material
Enzyme-free sensor
Glucose detection
Low-cost electrochemical sensor
Thermoplastic composite electrodes
topic Biodegradable material
Enzyme-free sensor
Glucose detection
Low-cost electrochemical sensor
Thermoplastic composite electrodes
description There is an increasing demand for robust, affordable, and sensitive analytical devices for clinical diagnostics. Herein, we describe a simple route to produce biodegradable and disposable thermoplastic composite electrodes using the commercial polymer (Ecoflex®) and graphite microparticles. The surface of the carbon composite electrode was modified with gold nanoparticles conjugated with graphene quantum dots (Au@GQDs) for sensitive and non-enzymatic glucose sensing. The morphological and structural characterizations of the modified electrochemical device revealed a hydrophilic rough composite surface, with high dispersion of graphite in the polymer matrix and confirmed the successful superficial modification of the working electrode with spherical Au@GQDs nanoparticles. Cyclic voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) were used to electrochemically characterize the modified and nonmodified thermoplastic sensor, demonstrating a 4.5-fold enhancement of the electroactive area, higher heterogeneous electron transfer kinetics, and electrocatalytic properties for glucose sensing when the sensor was modified with Au@GQDs. The electrochemical determination of the glucose was carried out using an amperometric method applying -0.15 V in alkaline conditions (0.1 mol L−1 NaOH). Under optimized conditions, the proposed method presented a high sensitivity (4.85 A L mol−1) and a low limit of detection (LOD) of 9.12 µmol L−1. The Ecoflex:graphite/Au@GQDs sensor was applied for glucose sensing in synthetic saliva samples with recovery values ranging from 97.84% to 105.20%, highlighting the excellent accuracy of the proposed method. Finally, the electrode was subjected to thermal and biological degradation studies and presented disposable and eco-friendly characteristics. Based on these results, we believe that this new thermoplastic composite sensor modified with Au@GQDs can be an excellent candidate for a disposable, low-cost, and enzymeless electrochemical glucose sensor.
publishDate 2022
dc.date.none.fl_str_mv 2022-11-01
2023-03-01T21:14:56Z
2023-03-01T21:14:56Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.snr.2022.100102
Sensors and Actuators Reports, v. 4.
2666-0539
http://hdl.handle.net/11449/241651
10.1016/j.snr.2022.100102
2-s2.0-85129615875
url http://dx.doi.org/10.1016/j.snr.2022.100102
http://hdl.handle.net/11449/241651
identifier_str_mv Sensors and Actuators Reports, v. 4.
2666-0539
10.1016/j.snr.2022.100102
2-s2.0-85129615875
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Sensors and Actuators Reports
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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