Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDs
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.snr.2022.100102 http://hdl.handle.net/11449/241651 |
Resumo: | There is an increasing demand for robust, affordable, and sensitive analytical devices for clinical diagnostics. Herein, we describe a simple route to produce biodegradable and disposable thermoplastic composite electrodes using the commercial polymer (Ecoflex®) and graphite microparticles. The surface of the carbon composite electrode was modified with gold nanoparticles conjugated with graphene quantum dots (Au@GQDs) for sensitive and non-enzymatic glucose sensing. The morphological and structural characterizations of the modified electrochemical device revealed a hydrophilic rough composite surface, with high dispersion of graphite in the polymer matrix and confirmed the successful superficial modification of the working electrode with spherical Au@GQDs nanoparticles. Cyclic voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) were used to electrochemically characterize the modified and nonmodified thermoplastic sensor, demonstrating a 4.5-fold enhancement of the electroactive area, higher heterogeneous electron transfer kinetics, and electrocatalytic properties for glucose sensing when the sensor was modified with Au@GQDs. The electrochemical determination of the glucose was carried out using an amperometric method applying -0.15 V in alkaline conditions (0.1 mol L−1 NaOH). Under optimized conditions, the proposed method presented a high sensitivity (4.85 A L mol−1) and a low limit of detection (LOD) of 9.12 µmol L−1. The Ecoflex:graphite/Au@GQDs sensor was applied for glucose sensing in synthetic saliva samples with recovery values ranging from 97.84% to 105.20%, highlighting the excellent accuracy of the proposed method. Finally, the electrode was subjected to thermal and biological degradation studies and presented disposable and eco-friendly characteristics. Based on these results, we believe that this new thermoplastic composite sensor modified with Au@GQDs can be an excellent candidate for a disposable, low-cost, and enzymeless electrochemical glucose sensor. |
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Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDsBiodegradable materialEnzyme-free sensorGlucose detectionLow-cost electrochemical sensorThermoplastic composite electrodesThere is an increasing demand for robust, affordable, and sensitive analytical devices for clinical diagnostics. Herein, we describe a simple route to produce biodegradable and disposable thermoplastic composite electrodes using the commercial polymer (Ecoflex®) and graphite microparticles. The surface of the carbon composite electrode was modified with gold nanoparticles conjugated with graphene quantum dots (Au@GQDs) for sensitive and non-enzymatic glucose sensing. The morphological and structural characterizations of the modified electrochemical device revealed a hydrophilic rough composite surface, with high dispersion of graphite in the polymer matrix and confirmed the successful superficial modification of the working electrode with spherical Au@GQDs nanoparticles. Cyclic voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) were used to electrochemically characterize the modified and nonmodified thermoplastic sensor, demonstrating a 4.5-fold enhancement of the electroactive area, higher heterogeneous electron transfer kinetics, and electrocatalytic properties for glucose sensing when the sensor was modified with Au@GQDs. The electrochemical determination of the glucose was carried out using an amperometric method applying -0.15 V in alkaline conditions (0.1 mol L−1 NaOH). Under optimized conditions, the proposed method presented a high sensitivity (4.85 A L mol−1) and a low limit of detection (LOD) of 9.12 µmol L−1. The Ecoflex:graphite/Au@GQDs sensor was applied for glucose sensing in synthetic saliva samples with recovery values ranging from 97.84% to 105.20%, highlighting the excellent accuracy of the proposed method. Finally, the electrode was subjected to thermal and biological degradation studies and presented disposable and eco-friendly characteristics. Based on these results, we believe that this new thermoplastic composite sensor modified with Au@GQDs can be an excellent candidate for a disposable, low-cost, and enzymeless electrochemical glucose sensor.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)American Sleep Medicine FoundationCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Portable Chemical Sensors Lab Department of Analytical Chemistry Institute of Chemistry State University of Campinas – UNICAMP, P.O. Box 6154, São PauloPolymers and Biopolymers Technology Lab. (TecPBio) Institute of Science and Technology (ICT) Federal University of São Paulo (UNIFESP), SPInstitute of Science and Technology São Paulo State University (UNESP)Center of Science and Technology for Sustainability (CCTS) Federal University of São Carlos (UFSCar), SPInstitute of Science and Technology São Paulo State University (UNESP)FAPESP: 2016/19387FAPESP: 2018/04061-8FAPESP: 2018/08782-1FAPESP: 2019/02692-3American Sleep Medicine Foundation: 88882.430936/2019-01CAPES: 88887.497915/2020-00Universidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Universidade Estadual Paulista (UNESP)Universidade Federal de São Carlos (UFSCar)de Lima, Lucas F.de Freitas, Amanda de S.M.Ferreira, André L.Maciel, Cristiane Carla [UNESP]Ferreira, Marystelade Araujo, William R.2023-03-01T21:14:56Z2023-03-01T21:14:56Z2022-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.snr.2022.100102Sensors and Actuators Reports, v. 4.2666-0539http://hdl.handle.net/11449/24165110.1016/j.snr.2022.1001022-s2.0-85129615875Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengSensors and Actuators Reportsinfo:eu-repo/semantics/openAccess2023-03-01T21:14:57Zoai:repositorio.unesp.br:11449/241651Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T21:14:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDs |
title |
Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDs |
spellingShingle |
Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDs de Lima, Lucas F. Biodegradable material Enzyme-free sensor Glucose detection Low-cost electrochemical sensor Thermoplastic composite electrodes |
title_short |
Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDs |
title_full |
Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDs |
title_fullStr |
Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDs |
title_full_unstemmed |
Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDs |
title_sort |
Enzymeless glucose sensor based on disposable Ecoflex®/graphite thermoplastic composite substrate modified with Au@GQDs |
author |
de Lima, Lucas F. |
author_facet |
de Lima, Lucas F. de Freitas, Amanda de S.M. Ferreira, André L. Maciel, Cristiane Carla [UNESP] Ferreira, Marystela de Araujo, William R. |
author_role |
author |
author2 |
de Freitas, Amanda de S.M. Ferreira, André L. Maciel, Cristiane Carla [UNESP] Ferreira, Marystela de Araujo, William R. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade de São Paulo (USP) Universidade Estadual Paulista (UNESP) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
de Lima, Lucas F. de Freitas, Amanda de S.M. Ferreira, André L. Maciel, Cristiane Carla [UNESP] Ferreira, Marystela de Araujo, William R. |
dc.subject.por.fl_str_mv |
Biodegradable material Enzyme-free sensor Glucose detection Low-cost electrochemical sensor Thermoplastic composite electrodes |
topic |
Biodegradable material Enzyme-free sensor Glucose detection Low-cost electrochemical sensor Thermoplastic composite electrodes |
description |
There is an increasing demand for robust, affordable, and sensitive analytical devices for clinical diagnostics. Herein, we describe a simple route to produce biodegradable and disposable thermoplastic composite electrodes using the commercial polymer (Ecoflex®) and graphite microparticles. The surface of the carbon composite electrode was modified with gold nanoparticles conjugated with graphene quantum dots (Au@GQDs) for sensitive and non-enzymatic glucose sensing. The morphological and structural characterizations of the modified electrochemical device revealed a hydrophilic rough composite surface, with high dispersion of graphite in the polymer matrix and confirmed the successful superficial modification of the working electrode with spherical Au@GQDs nanoparticles. Cyclic voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS) were used to electrochemically characterize the modified and nonmodified thermoplastic sensor, demonstrating a 4.5-fold enhancement of the electroactive area, higher heterogeneous electron transfer kinetics, and electrocatalytic properties for glucose sensing when the sensor was modified with Au@GQDs. The electrochemical determination of the glucose was carried out using an amperometric method applying -0.15 V in alkaline conditions (0.1 mol L−1 NaOH). Under optimized conditions, the proposed method presented a high sensitivity (4.85 A L mol−1) and a low limit of detection (LOD) of 9.12 µmol L−1. The Ecoflex:graphite/Au@GQDs sensor was applied for glucose sensing in synthetic saliva samples with recovery values ranging from 97.84% to 105.20%, highlighting the excellent accuracy of the proposed method. Finally, the electrode was subjected to thermal and biological degradation studies and presented disposable and eco-friendly characteristics. Based on these results, we believe that this new thermoplastic composite sensor modified with Au@GQDs can be an excellent candidate for a disposable, low-cost, and enzymeless electrochemical glucose sensor. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-01 2023-03-01T21:14:56Z 2023-03-01T21:14:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.snr.2022.100102 Sensors and Actuators Reports, v. 4. 2666-0539 http://hdl.handle.net/11449/241651 10.1016/j.snr.2022.100102 2-s2.0-85129615875 |
url |
http://dx.doi.org/10.1016/j.snr.2022.100102 http://hdl.handle.net/11449/241651 |
identifier_str_mv |
Sensors and Actuators Reports, v. 4. 2666-0539 10.1016/j.snr.2022.100102 2-s2.0-85129615875 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Sensors and Actuators Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965488500965376 |