Cutaneous hypersensitivity induced in rabbits by extracts of the tick Amblyomma cajennense (Acari: Ixodidae)

Detalhes bibliográficos
Autor(a) principal: Hlatshwayo, M.
Data de Publicação: 2004
Outros Autores: Szabó, M. J.P., Bechara, G. H., Mbati, P. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.4102/jsava.v75i1.446
http://jsava.co.za/index.php/jsava/article/view/446
http://hdl.handle.net/11449/243625
Resumo: The cutaneous hypersensitivity test was used to correlate host resistance to ticks and type of reactions elicited by Amblyomma cajennense (Fabricius, 1787) tick extract in rabbits. Rabbits were divided into 3 groups of 2 animals each: naive, pre-infested and control. Cutaneous hypersensitivity was induced by intradermal inoculation of 25 μg extract in 0.03 ml of phosphate buffered saline (PBS) in rabbit ears. Control rabbits were inoculated with PBS only. The ear thickness was measured with a Mitutoyo® device before and 10 min, 1, 2, 4, 18, 24, 48, 72 and 96 h post-inoculation (PI). Pre-infested rabbits showed an immediate type reaction within the 1st 10 min PI (60% increase in ear thickness) and a delayed reaction (18 h) (85 % increase), whereas the naive rabbits showed only the immediate reaction within the 1st 4 h (60 % increase). PBS induced only mild reactions. These results point out the crucial role of the cellular immune response of rabbits in the expression of resistance to A. cajennense.
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spelling Cutaneous hypersensitivity induced in rabbits by extracts of the tick Amblyomma cajennense (Acari: Ixodidae)Amblyomma cajennenseCutaneous hypersensitivityTick extractThe cutaneous hypersensitivity test was used to correlate host resistance to ticks and type of reactions elicited by Amblyomma cajennense (Fabricius, 1787) tick extract in rabbits. Rabbits were divided into 3 groups of 2 animals each: naive, pre-infested and control. Cutaneous hypersensitivity was induced by intradermal inoculation of 25 μg extract in 0.03 ml of phosphate buffered saline (PBS) in rabbit ears. Control rabbits were inoculated with PBS only. The ear thickness was measured with a Mitutoyo® device before and 10 min, 1, 2, 4, 18, 24, 48, 72 and 96 h post-inoculation (PI). Pre-infested rabbits showed an immediate type reaction within the 1st 10 min PI (60% increase in ear thickness) and a delayed reaction (18 h) (85 % increase), whereas the naive rabbits showed only the immediate reaction within the 1st 4 h (60 % increase). PBS induced only mild reactions. These results point out the crucial role of the cellular immune response of rabbits in the expression of resistance to A. cajennense.Parasitology Research Programme Department of Zoology/Entomology University of the Free State, Private Bag X13, Phuthaditjhaba 9866Department of Animal Pathology Fac. of Agrarian/Veterinary Sciences State University of São Paulo, 14.870-000 Jaboticabal-SPUniv Free State, Parasitol Res Programme, Dept Zool & Entomol, ZA-9866 Phuthaditjhaba, South AfricaState Univ São Paulo, Dept Anim Pathol, Fac Agr & Vet Sci, BR-14870000 Jaboticabal, SP, BrazilState Univ São Paulo, Dept Anim Pathol, Fac Agr & Vet Sci, BR-14870000 Jaboticabal, SP, BrazilSouth African Vet AssnUniversity of the Free StateUniversidade de São Paulo (USP)Universidade Estadual Paulista (Unesp)Hlatshwayo, M.Szabó, M. J.P.Bechara, G. H.Mbati, P. A.2022-04-29T08:43:43Z2014-05-20T15:24:22Z2022-04-29T08:43:43Z2014-05-20T15:24:22Z2004-06-18info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article37-39application/pdfhttp://dx.doi.org/10.4102/jsava.v75i1.446http://jsava.co.za/index.php/jsava/article/view/446Journal of the South African Veterinary Association, v. 75, n. 1, p. 37-39, 2004.Journal of the South African Veterinary Association-tydskrif Van Die Suid-afrikaanse Veterinere Vereniging. Monument Park: South African Vet Assn, v. 75, n. 1, p. 37-39, 2004.2224-94351019-9128http://hdl.handle.net/11449/24362510.4102/jsava.v75i1.446WOS:0002221453000082-s2.0-4644247080WOS000222145300008.pdf2382374201685423ScopusWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of the South African Veterinary AssociationJournal of the South African Veterinary Association-tydskrif Van Die Suid-afrikaanse Veterinere Vereniginginfo:eu-repo/semantics/openAccess2024-06-07T13:03:19Zoai:repositorio.unesp.br:11449/243625Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:13:51.000457Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cutaneous hypersensitivity induced in rabbits by extracts of the tick Amblyomma cajennense (Acari: Ixodidae)
title Cutaneous hypersensitivity induced in rabbits by extracts of the tick Amblyomma cajennense (Acari: Ixodidae)
spellingShingle Cutaneous hypersensitivity induced in rabbits by extracts of the tick Amblyomma cajennense (Acari: Ixodidae)
Hlatshwayo, M.
Amblyomma cajennense
Cutaneous hypersensitivity
Tick extract
title_short Cutaneous hypersensitivity induced in rabbits by extracts of the tick Amblyomma cajennense (Acari: Ixodidae)
title_full Cutaneous hypersensitivity induced in rabbits by extracts of the tick Amblyomma cajennense (Acari: Ixodidae)
title_fullStr Cutaneous hypersensitivity induced in rabbits by extracts of the tick Amblyomma cajennense (Acari: Ixodidae)
title_full_unstemmed Cutaneous hypersensitivity induced in rabbits by extracts of the tick Amblyomma cajennense (Acari: Ixodidae)
title_sort Cutaneous hypersensitivity induced in rabbits by extracts of the tick Amblyomma cajennense (Acari: Ixodidae)
author Hlatshwayo, M.
author_facet Hlatshwayo, M.
Szabó, M. J.P.
Bechara, G. H.
Mbati, P. A.
author_role author
author2 Szabó, M. J.P.
Bechara, G. H.
Mbati, P. A.
author2_role author
author
author
dc.contributor.none.fl_str_mv University of the Free State
Universidade de São Paulo (USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Hlatshwayo, M.
Szabó, M. J.P.
Bechara, G. H.
Mbati, P. A.
dc.subject.por.fl_str_mv Amblyomma cajennense
Cutaneous hypersensitivity
Tick extract
topic Amblyomma cajennense
Cutaneous hypersensitivity
Tick extract
description The cutaneous hypersensitivity test was used to correlate host resistance to ticks and type of reactions elicited by Amblyomma cajennense (Fabricius, 1787) tick extract in rabbits. Rabbits were divided into 3 groups of 2 animals each: naive, pre-infested and control. Cutaneous hypersensitivity was induced by intradermal inoculation of 25 μg extract in 0.03 ml of phosphate buffered saline (PBS) in rabbit ears. Control rabbits were inoculated with PBS only. The ear thickness was measured with a Mitutoyo® device before and 10 min, 1, 2, 4, 18, 24, 48, 72 and 96 h post-inoculation (PI). Pre-infested rabbits showed an immediate type reaction within the 1st 10 min PI (60% increase in ear thickness) and a delayed reaction (18 h) (85 % increase), whereas the naive rabbits showed only the immediate reaction within the 1st 4 h (60 % increase). PBS induced only mild reactions. These results point out the crucial role of the cellular immune response of rabbits in the expression of resistance to A. cajennense.
publishDate 2004
dc.date.none.fl_str_mv 2004-06-18
2014-05-20T15:24:22Z
2014-05-20T15:24:22Z
2022-04-29T08:43:43Z
2022-04-29T08:43:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4102/jsava.v75i1.446
http://jsava.co.za/index.php/jsava/article/view/446
Journal of the South African Veterinary Association, v. 75, n. 1, p. 37-39, 2004.
Journal of the South African Veterinary Association-tydskrif Van Die Suid-afrikaanse Veterinere Vereniging. Monument Park: South African Vet Assn, v. 75, n. 1, p. 37-39, 2004.
2224-9435
1019-9128
http://hdl.handle.net/11449/243625
10.4102/jsava.v75i1.446
WOS:000222145300008
2-s2.0-4644247080
WOS000222145300008.pdf
2382374201685423
url http://dx.doi.org/10.4102/jsava.v75i1.446
http://jsava.co.za/index.php/jsava/article/view/446
http://hdl.handle.net/11449/243625
identifier_str_mv Journal of the South African Veterinary Association, v. 75, n. 1, p. 37-39, 2004.
Journal of the South African Veterinary Association-tydskrif Van Die Suid-afrikaanse Veterinere Vereniging. Monument Park: South African Vet Assn, v. 75, n. 1, p. 37-39, 2004.
2224-9435
1019-9128
10.4102/jsava.v75i1.446
WOS:000222145300008
2-s2.0-4644247080
WOS000222145300008.pdf
2382374201685423
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of the South African Veterinary Association
Journal of the South African Veterinary Association-tydskrif Van Die Suid-afrikaanse Veterinere Vereniging
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 37-39
application/pdf
dc.publisher.none.fl_str_mv South African Vet Assn
publisher.none.fl_str_mv South African Vet Assn
dc.source.none.fl_str_mv Scopus
Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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