In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agents
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.mrgentox.2016.01.007 http://hdl.handle.net/11449/172554 |
Resumo: | Tuberculosis is a top infectious disease killer worldwide, caused by the bacteria Mycobacterium tuberculosis. Increasing incidences of multiple drug-resistance (MDR) strains are emerging as one of the major public health threats. However, the drugs in use are still incapable of controlling the appalling upsurge of MDR. In recent years a marked number of research groups have devoted their attention toward the development of specific and cost-effective antimicrobial agents against targeted MDR-Tuberculosis. In previous studies, ruthenium(II) complexes (SCAR) have shown a promising activity against MDR-Tuberculosis although few studies have indeed considered ruthenium toxicity. Therefore, within the preclinical requirements, we have sought to determine the cyto-genotoxicity of three SCAR complexes in this present study. The treatment with the SCARs induced a concentration-dependent decrease in cell viability in CHO-K1 and HepG2 cells. Based on the clonogenic survival, SCAR 5 was found to be more cytotoxic while SCAR 6 exhibited selectivity action on tumor cells. Although SCAR 4 and 5 did not indicate any mutagenic activity as evidenced by the Ames and Cytokinesis block micronucleus cytome assays, the complex SCAR 6 was found to engender a frameshift mutation detected by Salmonella typhimurium in the presence of S9. Similarly, we observed a chromosomal damage in HepG2 cells with significant increases of micronuclei and nucleoplasmic bridges. These data indicate that SCAR 4 and 5 complexes did not show genotoxicity in our models while SCAR 6 was considered mutagenic. This study presented a comprehensive genotoxic evaluation of SCAR complexes were shown to be genotoxic in vitro. All in all, further studies are required to fully elucidate how the properties can affect human health. |
id |
UNSP_e19b5f41494e303e621073e154214aa3 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/172554 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agentsCisplatinCytotoxicityMutagenicityRuthenium(II) complexesTuberculosisTuberculosis is a top infectious disease killer worldwide, caused by the bacteria Mycobacterium tuberculosis. Increasing incidences of multiple drug-resistance (MDR) strains are emerging as one of the major public health threats. However, the drugs in use are still incapable of controlling the appalling upsurge of MDR. In recent years a marked number of research groups have devoted their attention toward the development of specific and cost-effective antimicrobial agents against targeted MDR-Tuberculosis. In previous studies, ruthenium(II) complexes (SCAR) have shown a promising activity against MDR-Tuberculosis although few studies have indeed considered ruthenium toxicity. Therefore, within the preclinical requirements, we have sought to determine the cyto-genotoxicity of three SCAR complexes in this present study. The treatment with the SCARs induced a concentration-dependent decrease in cell viability in CHO-K1 and HepG2 cells. Based on the clonogenic survival, SCAR 5 was found to be more cytotoxic while SCAR 6 exhibited selectivity action on tumor cells. Although SCAR 4 and 5 did not indicate any mutagenic activity as evidenced by the Ames and Cytokinesis block micronucleus cytome assays, the complex SCAR 6 was found to engender a frameshift mutation detected by Salmonella typhimurium in the presence of S9. Similarly, we observed a chromosomal damage in HepG2 cells with significant increases of micronuclei and nucleoplasmic bridges. These data indicate that SCAR 4 and 5 complexes did not show genotoxicity in our models while SCAR 6 was considered mutagenic. This study presented a comprehensive genotoxic evaluation of SCAR complexes were shown to be genotoxic in vitro. All in all, further studies are required to fully elucidate how the properties can affect human health.Department of Biological Sciences Faculty of Pharmaceutical Sciences of Araraquara São Paulo State University UNESPDepartment of Biological Sciences and Health Centro Universitário de Araraquara UNIARADepartment of Inorganic Chemistry Federal University of São Carlos UFSCAR São CarlosDepartment of Biological Sciences Faculty of Pharmaceutical Sciences of Araraquara São Paulo State University UNESPUniversidade Estadual Paulista (Unesp)UNIARAUniversidade Federal de São Carlos (UFSCar)De Grandis, Rone Aparecido [UNESP]Resende, Flávia Aparecidada Silva, Monize MartinsPavan, Fernando Rogério [UNESP]Batista, Alzir AzevedoVaranda, Eliana Aparecida [UNESP]2018-12-11T17:00:57Z2018-12-11T17:00:57Z2016-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11-18application/pdfhttp://dx.doi.org/10.1016/j.mrgentox.2016.01.007Mutation Research - Genetic Toxicology and Environmental Mutagenesis, v. 798-799, p. 11-18.1879-35921383-5718http://hdl.handle.net/11449/17255410.1016/j.mrgentox.2016.01.0072-s2.0-849585526692-s2.0-84958552669.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMutation Research - Genetic Toxicology and Environmental Mutagenesis0,747info:eu-repo/semantics/openAccess2024-06-24T13:07:13Zoai:repositorio.unesp.br:11449/172554Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:22:18.041285Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agents |
title |
In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agents |
spellingShingle |
In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agents De Grandis, Rone Aparecido [UNESP] Cisplatin Cytotoxicity Mutagenicity Ruthenium(II) complexes Tuberculosis |
title_short |
In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agents |
title_full |
In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agents |
title_fullStr |
In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agents |
title_full_unstemmed |
In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agents |
title_sort |
In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agents |
author |
De Grandis, Rone Aparecido [UNESP] |
author_facet |
De Grandis, Rone Aparecido [UNESP] Resende, Flávia Aparecida da Silva, Monize Martins Pavan, Fernando Rogério [UNESP] Batista, Alzir Azevedo Varanda, Eliana Aparecida [UNESP] |
author_role |
author |
author2 |
Resende, Flávia Aparecida da Silva, Monize Martins Pavan, Fernando Rogério [UNESP] Batista, Alzir Azevedo Varanda, Eliana Aparecida [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) UNIARA Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
De Grandis, Rone Aparecido [UNESP] Resende, Flávia Aparecida da Silva, Monize Martins Pavan, Fernando Rogério [UNESP] Batista, Alzir Azevedo Varanda, Eliana Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
Cisplatin Cytotoxicity Mutagenicity Ruthenium(II) complexes Tuberculosis |
topic |
Cisplatin Cytotoxicity Mutagenicity Ruthenium(II) complexes Tuberculosis |
description |
Tuberculosis is a top infectious disease killer worldwide, caused by the bacteria Mycobacterium tuberculosis. Increasing incidences of multiple drug-resistance (MDR) strains are emerging as one of the major public health threats. However, the drugs in use are still incapable of controlling the appalling upsurge of MDR. In recent years a marked number of research groups have devoted their attention toward the development of specific and cost-effective antimicrobial agents against targeted MDR-Tuberculosis. In previous studies, ruthenium(II) complexes (SCAR) have shown a promising activity against MDR-Tuberculosis although few studies have indeed considered ruthenium toxicity. Therefore, within the preclinical requirements, we have sought to determine the cyto-genotoxicity of three SCAR complexes in this present study. The treatment with the SCARs induced a concentration-dependent decrease in cell viability in CHO-K1 and HepG2 cells. Based on the clonogenic survival, SCAR 5 was found to be more cytotoxic while SCAR 6 exhibited selectivity action on tumor cells. Although SCAR 4 and 5 did not indicate any mutagenic activity as evidenced by the Ames and Cytokinesis block micronucleus cytome assays, the complex SCAR 6 was found to engender a frameshift mutation detected by Salmonella typhimurium in the presence of S9. Similarly, we observed a chromosomal damage in HepG2 cells with significant increases of micronuclei and nucleoplasmic bridges. These data indicate that SCAR 4 and 5 complexes did not show genotoxicity in our models while SCAR 6 was considered mutagenic. This study presented a comprehensive genotoxic evaluation of SCAR complexes were shown to be genotoxic in vitro. All in all, further studies are required to fully elucidate how the properties can affect human health. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03-01 2018-12-11T17:00:57Z 2018-12-11T17:00:57Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.mrgentox.2016.01.007 Mutation Research - Genetic Toxicology and Environmental Mutagenesis, v. 798-799, p. 11-18. 1879-3592 1383-5718 http://hdl.handle.net/11449/172554 10.1016/j.mrgentox.2016.01.007 2-s2.0-84958552669 2-s2.0-84958552669.pdf |
url |
http://dx.doi.org/10.1016/j.mrgentox.2016.01.007 http://hdl.handle.net/11449/172554 |
identifier_str_mv |
Mutation Research - Genetic Toxicology and Environmental Mutagenesis, v. 798-799, p. 11-18. 1879-3592 1383-5718 10.1016/j.mrgentox.2016.01.007 2-s2.0-84958552669 2-s2.0-84958552669.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mutation Research - Genetic Toxicology and Environmental Mutagenesis 0,747 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11-18 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128221391093760 |