In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agents

Detalhes bibliográficos
Autor(a) principal: De Grandis, Rone Aparecido [UNESP]
Data de Publicação: 2016
Outros Autores: Resende, Flávia Aparecida, da Silva, Monize Martins, Pavan, Fernando Rogério [UNESP], Batista, Alzir Azevedo, Varanda, Eliana Aparecida [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.mrgentox.2016.01.007
http://hdl.handle.net/11449/172554
Resumo: Tuberculosis is a top infectious disease killer worldwide, caused by the bacteria Mycobacterium tuberculosis. Increasing incidences of multiple drug-resistance (MDR) strains are emerging as one of the major public health threats. However, the drugs in use are still incapable of controlling the appalling upsurge of MDR. In recent years a marked number of research groups have devoted their attention toward the development of specific and cost-effective antimicrobial agents against targeted MDR-Tuberculosis. In previous studies, ruthenium(II) complexes (SCAR) have shown a promising activity against MDR-Tuberculosis although few studies have indeed considered ruthenium toxicity. Therefore, within the preclinical requirements, we have sought to determine the cyto-genotoxicity of three SCAR complexes in this present study. The treatment with the SCARs induced a concentration-dependent decrease in cell viability in CHO-K1 and HepG2 cells. Based on the clonogenic survival, SCAR 5 was found to be more cytotoxic while SCAR 6 exhibited selectivity action on tumor cells. Although SCAR 4 and 5 did not indicate any mutagenic activity as evidenced by the Ames and Cytokinesis block micronucleus cytome assays, the complex SCAR 6 was found to engender a frameshift mutation detected by Salmonella typhimurium in the presence of S9. Similarly, we observed a chromosomal damage in HepG2 cells with significant increases of micronuclei and nucleoplasmic bridges. These data indicate that SCAR 4 and 5 complexes did not show genotoxicity in our models while SCAR 6 was considered mutagenic. This study presented a comprehensive genotoxic evaluation of SCAR complexes were shown to be genotoxic in vitro. All in all, further studies are required to fully elucidate how the properties can affect human health.
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spelling In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agentsCisplatinCytotoxicityMutagenicityRuthenium(II) complexesTuberculosisTuberculosis is a top infectious disease killer worldwide, caused by the bacteria Mycobacterium tuberculosis. Increasing incidences of multiple drug-resistance (MDR) strains are emerging as one of the major public health threats. However, the drugs in use are still incapable of controlling the appalling upsurge of MDR. In recent years a marked number of research groups have devoted their attention toward the development of specific and cost-effective antimicrobial agents against targeted MDR-Tuberculosis. In previous studies, ruthenium(II) complexes (SCAR) have shown a promising activity against MDR-Tuberculosis although few studies have indeed considered ruthenium toxicity. Therefore, within the preclinical requirements, we have sought to determine the cyto-genotoxicity of three SCAR complexes in this present study. The treatment with the SCARs induced a concentration-dependent decrease in cell viability in CHO-K1 and HepG2 cells. Based on the clonogenic survival, SCAR 5 was found to be more cytotoxic while SCAR 6 exhibited selectivity action on tumor cells. Although SCAR 4 and 5 did not indicate any mutagenic activity as evidenced by the Ames and Cytokinesis block micronucleus cytome assays, the complex SCAR 6 was found to engender a frameshift mutation detected by Salmonella typhimurium in the presence of S9. Similarly, we observed a chromosomal damage in HepG2 cells with significant increases of micronuclei and nucleoplasmic bridges. These data indicate that SCAR 4 and 5 complexes did not show genotoxicity in our models while SCAR 6 was considered mutagenic. This study presented a comprehensive genotoxic evaluation of SCAR complexes were shown to be genotoxic in vitro. All in all, further studies are required to fully elucidate how the properties can affect human health.Department of Biological Sciences Faculty of Pharmaceutical Sciences of Araraquara São Paulo State University UNESPDepartment of Biological Sciences and Health Centro Universitário de Araraquara UNIARADepartment of Inorganic Chemistry Federal University of São Carlos UFSCAR São CarlosDepartment of Biological Sciences Faculty of Pharmaceutical Sciences of Araraquara São Paulo State University UNESPUniversidade Estadual Paulista (Unesp)UNIARAUniversidade Federal de São Carlos (UFSCar)De Grandis, Rone Aparecido [UNESP]Resende, Flávia Aparecidada Silva, Monize MartinsPavan, Fernando Rogério [UNESP]Batista, Alzir AzevedoVaranda, Eliana Aparecida [UNESP]2018-12-11T17:00:57Z2018-12-11T17:00:57Z2016-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11-18application/pdfhttp://dx.doi.org/10.1016/j.mrgentox.2016.01.007Mutation Research - Genetic Toxicology and Environmental Mutagenesis, v. 798-799, p. 11-18.1879-35921383-5718http://hdl.handle.net/11449/17255410.1016/j.mrgentox.2016.01.0072-s2.0-849585526692-s2.0-84958552669.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMutation Research - Genetic Toxicology and Environmental Mutagenesis0,747info:eu-repo/semantics/openAccess2024-06-24T13:07:13Zoai:repositorio.unesp.br:11449/172554Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:22:18.041285Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agents
title In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agents
spellingShingle In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agents
De Grandis, Rone Aparecido [UNESP]
Cisplatin
Cytotoxicity
Mutagenicity
Ruthenium(II) complexes
Tuberculosis
title_short In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agents
title_full In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agents
title_fullStr In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agents
title_full_unstemmed In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agents
title_sort In vitro evaluation of the cyto-genotoxic potential of Ruthenium(II) SCAR complexes: A promising class of antituberculosis agents
author De Grandis, Rone Aparecido [UNESP]
author_facet De Grandis, Rone Aparecido [UNESP]
Resende, Flávia Aparecida
da Silva, Monize Martins
Pavan, Fernando Rogério [UNESP]
Batista, Alzir Azevedo
Varanda, Eliana Aparecida [UNESP]
author_role author
author2 Resende, Flávia Aparecida
da Silva, Monize Martins
Pavan, Fernando Rogério [UNESP]
Batista, Alzir Azevedo
Varanda, Eliana Aparecida [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
UNIARA
Universidade Federal de São Carlos (UFSCar)
dc.contributor.author.fl_str_mv De Grandis, Rone Aparecido [UNESP]
Resende, Flávia Aparecida
da Silva, Monize Martins
Pavan, Fernando Rogério [UNESP]
Batista, Alzir Azevedo
Varanda, Eliana Aparecida [UNESP]
dc.subject.por.fl_str_mv Cisplatin
Cytotoxicity
Mutagenicity
Ruthenium(II) complexes
Tuberculosis
topic Cisplatin
Cytotoxicity
Mutagenicity
Ruthenium(II) complexes
Tuberculosis
description Tuberculosis is a top infectious disease killer worldwide, caused by the bacteria Mycobacterium tuberculosis. Increasing incidences of multiple drug-resistance (MDR) strains are emerging as one of the major public health threats. However, the drugs in use are still incapable of controlling the appalling upsurge of MDR. In recent years a marked number of research groups have devoted their attention toward the development of specific and cost-effective antimicrobial agents against targeted MDR-Tuberculosis. In previous studies, ruthenium(II) complexes (SCAR) have shown a promising activity against MDR-Tuberculosis although few studies have indeed considered ruthenium toxicity. Therefore, within the preclinical requirements, we have sought to determine the cyto-genotoxicity of three SCAR complexes in this present study. The treatment with the SCARs induced a concentration-dependent decrease in cell viability in CHO-K1 and HepG2 cells. Based on the clonogenic survival, SCAR 5 was found to be more cytotoxic while SCAR 6 exhibited selectivity action on tumor cells. Although SCAR 4 and 5 did not indicate any mutagenic activity as evidenced by the Ames and Cytokinesis block micronucleus cytome assays, the complex SCAR 6 was found to engender a frameshift mutation detected by Salmonella typhimurium in the presence of S9. Similarly, we observed a chromosomal damage in HepG2 cells with significant increases of micronuclei and nucleoplasmic bridges. These data indicate that SCAR 4 and 5 complexes did not show genotoxicity in our models while SCAR 6 was considered mutagenic. This study presented a comprehensive genotoxic evaluation of SCAR complexes were shown to be genotoxic in vitro. All in all, further studies are required to fully elucidate how the properties can affect human health.
publishDate 2016
dc.date.none.fl_str_mv 2016-03-01
2018-12-11T17:00:57Z
2018-12-11T17:00:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.mrgentox.2016.01.007
Mutation Research - Genetic Toxicology and Environmental Mutagenesis, v. 798-799, p. 11-18.
1879-3592
1383-5718
http://hdl.handle.net/11449/172554
10.1016/j.mrgentox.2016.01.007
2-s2.0-84958552669
2-s2.0-84958552669.pdf
url http://dx.doi.org/10.1016/j.mrgentox.2016.01.007
http://hdl.handle.net/11449/172554
identifier_str_mv Mutation Research - Genetic Toxicology and Environmental Mutagenesis, v. 798-799, p. 11-18.
1879-3592
1383-5718
10.1016/j.mrgentox.2016.01.007
2-s2.0-84958552669
2-s2.0-84958552669.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mutation Research - Genetic Toxicology and Environmental Mutagenesis
0,747
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11-18
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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