Biofilm formation by prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes

Detalhes bibliográficos
Autor(a) principal: Ely, Valessa Lunkes
Data de Publicação: 2019
Outros Autores: Gressler, Letícia Trevisan, Sutili, Fernando Jonas, Ribeiro, Márcio Garcia [UNESP], da Costa, Mateus Matiuzzi, de Vargas, Agueda Castagna, Botton, Sônia de Avila
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/0103-8478cr20180574
http://hdl.handle.net/11449/188944
Resumo: Prototheca spp. have been reported as an emergent environmental mastitis pathogen in several countries. Biofilm formation is a significant factor associated with different degrees of virulence developed by many microorganisms, including Prototheca spp. The present study aimed to compare two growth conditions and two staining dyes to determine which combination was more appropriate to evaluate qualitatively and quantitatively the production of biofilm by P. zopfii. Biofilm formation was evaluated in polystyrene microplates under static and dynamic growth conditions and staining with crystal violet or cotton blue dye. All P. zopfii isolates from cows with mastitis were classified as biofilm-producers in all growth conditions and staining. The cotton blue dye proved to be more appropriate method to classify the intensity of P. zopfii biofilm production.
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spelling Biofilm formation by prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyesFormação de biofilme por prototheca zopfii isolados de mastite bovina clínica e subclínica em distintas condições de crescimento sob diferentes corantesBovine mastitisDairy cattleIntramammary infectionsMicroplate biofilm assayPrototheca zopfiiVirulence factorPrototheca spp. have been reported as an emergent environmental mastitis pathogen in several countries. Biofilm formation is a significant factor associated with different degrees of virulence developed by many microorganisms, including Prototheca spp. The present study aimed to compare two growth conditions and two staining dyes to determine which combination was more appropriate to evaluate qualitatively and quantitatively the production of biofilm by P. zopfii. Biofilm formation was evaluated in polystyrene microplates under static and dynamic growth conditions and staining with crystal violet or cotton blue dye. All P. zopfii isolates from cows with mastitis were classified as biofilm-producers in all growth conditions and staining. The cotton blue dye proved to be more appropriate method to classify the intensity of P. zopfii biofilm production.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado do Rio Grande do SulDepartamento de Medicina Veterinária Preventiva (DMVP) Centro de Ciências Rurais (CCR) Universidade Federal de Santa Maria (UFSM)Laboratório de Microbiologia e Doenças Infecciosas Instituto Federal Farroupilha (IFFar)Departamento de Fisiologia e Farmacologia Universidade Federal de Santa Maria (UFSM)Departamento de Higiene Veterinária e Saúde Pública Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista Julio Mesquita Filho (UNESP)Departamento de Higiene Veterinária e Saúde Pública Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista Julio Mesquita Filho (UNESP)Universidade Federal de Sergipe (UFS)Instituto Federal Farroupilha (IFFar)Universidade Estadual Paulista (Unesp)Ely, Valessa LunkesGressler, Letícia TrevisanSutili, Fernando JonasRibeiro, Márcio Garcia [UNESP]da Costa, Mateus Matiuzzide Vargas, Agueda CastagnaBotton, Sônia de Avila2019-10-06T16:24:46Z2019-10-06T16:24:46Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1590/0103-8478cr20180574Ciencia Rural, v. 49, n. 2, 2019.1678-45960103-8478http://hdl.handle.net/11449/18894410.1590/0103-8478cr20180574S0103-847820190002004512-s2.0-85063982888S0103-84782019000200451.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCiencia Ruralinfo:eu-repo/semantics/openAccess2024-01-29T06:24:06Zoai:repositorio.unesp.br:11449/188944Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:11:27.366309Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Biofilm formation by prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes
Formação de biofilme por prototheca zopfii isolados de mastite bovina clínica e subclínica em distintas condições de crescimento sob diferentes corantes
title Biofilm formation by prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes
spellingShingle Biofilm formation by prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes
Ely, Valessa Lunkes
Bovine mastitis
Dairy cattle
Intramammary infections
Microplate biofilm assay
Prototheca zopfii
Virulence factor
title_short Biofilm formation by prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes
title_full Biofilm formation by prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes
title_fullStr Biofilm formation by prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes
title_full_unstemmed Biofilm formation by prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes
title_sort Biofilm formation by prototheca zopfii isolated from clinical and subclinical bovine mastitis in distinct growth conditions under different dyes
author Ely, Valessa Lunkes
author_facet Ely, Valessa Lunkes
Gressler, Letícia Trevisan
Sutili, Fernando Jonas
Ribeiro, Márcio Garcia [UNESP]
da Costa, Mateus Matiuzzi
de Vargas, Agueda Castagna
Botton, Sônia de Avila
author_role author
author2 Gressler, Letícia Trevisan
Sutili, Fernando Jonas
Ribeiro, Márcio Garcia [UNESP]
da Costa, Mateus Matiuzzi
de Vargas, Agueda Castagna
Botton, Sônia de Avila
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal de Sergipe (UFS)
Instituto Federal Farroupilha (IFFar)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Ely, Valessa Lunkes
Gressler, Letícia Trevisan
Sutili, Fernando Jonas
Ribeiro, Márcio Garcia [UNESP]
da Costa, Mateus Matiuzzi
de Vargas, Agueda Castagna
Botton, Sônia de Avila
dc.subject.por.fl_str_mv Bovine mastitis
Dairy cattle
Intramammary infections
Microplate biofilm assay
Prototheca zopfii
Virulence factor
topic Bovine mastitis
Dairy cattle
Intramammary infections
Microplate biofilm assay
Prototheca zopfii
Virulence factor
description Prototheca spp. have been reported as an emergent environmental mastitis pathogen in several countries. Biofilm formation is a significant factor associated with different degrees of virulence developed by many microorganisms, including Prototheca spp. The present study aimed to compare two growth conditions and two staining dyes to determine which combination was more appropriate to evaluate qualitatively and quantitatively the production of biofilm by P. zopfii. Biofilm formation was evaluated in polystyrene microplates under static and dynamic growth conditions and staining with crystal violet or cotton blue dye. All P. zopfii isolates from cows with mastitis were classified as biofilm-producers in all growth conditions and staining. The cotton blue dye proved to be more appropriate method to classify the intensity of P. zopfii biofilm production.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:24:46Z
2019-10-06T16:24:46Z
2019-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/0103-8478cr20180574
Ciencia Rural, v. 49, n. 2, 2019.
1678-4596
0103-8478
http://hdl.handle.net/11449/188944
10.1590/0103-8478cr20180574
S0103-84782019000200451
2-s2.0-85063982888
S0103-84782019000200451.pdf
url http://dx.doi.org/10.1590/0103-8478cr20180574
http://hdl.handle.net/11449/188944
identifier_str_mv Ciencia Rural, v. 49, n. 2, 2019.
1678-4596
0103-8478
10.1590/0103-8478cr20180574
S0103-84782019000200451
2-s2.0-85063982888
S0103-84782019000200451.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Ciencia Rural
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129593277677568

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