Hematopoietic Chimera in a Male Blood Donor and His Dizygotic Twin Sister

Detalhes bibliográficos
Autor(a) principal: Miola, Marcos P.
Data de Publicação: 2019
Outros Autores: Lopes, Alessandro G. [UNESP], Silva, Alessandra P., Gomes, Edney G. C., Machado, Leticia A. F., Veloso, Wanessa A., Costa, Carlos A., Fachini, Roberta M., Ricci Junior, Octavio, Brandao de Mattos, Cinara C., Mattos, Luiz Carlos de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1159/000495583
http://hdl.handle.net/11449/196325
Resumo: Twin hematopoietic chimera in humans is a phenomenon that was discovered accidentally and the prevalence of which remains unclear. The resolution of chimera cases requires studying family medical records, data analysis, and investigations of hematopoietic cells and cells from other tissues. The interactions among ABO, Lewis, and secretor histo-blood group systems are explored to resolve cases of hematopoietic chimera. Here we report a rare case of hematopoietic chimera where twins present a mixed field reaction in the ABO, Rh, and Kidd red blood cell phenotyping. Using red blood cells separated from the mixed field as well as molecular approaches and investigations of family members, we identify inconsistent genotypes with the Mendelian inheritance pattern when comparing the peripheral blood with the buccal epithelium of the male twin and his twin sister. Analysis of the ABO, Lewis, and secretor phenotypes, and genomic DNA from buccal epithelium showed the genotypes ABO*A1.01/ABO*B.01 and FUT2*01N.02/FUT2*01N.02 in the male twin and the genotypes ABO*O.01.01/ABO*O.01.02 and FUT2*01/FUT2*01 in the female twin. The results of the HLA-DRB1 genotyping showed inconsistency between the male and his twin sister. We conclude that the serological analyses combined with molecular approaches used in this study are good tools to resolve cases of hematopoietic chimera. (C) 2019 S. Karger AG, Basel
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spelling Hematopoietic Chimera in a Male Blood Donor and His Dizygotic Twin SisterChimerismDizygotic twinsBlood groupsPhenotypingMolecular analysisTwin hematopoietic chimera in humans is a phenomenon that was discovered accidentally and the prevalence of which remains unclear. The resolution of chimera cases requires studying family medical records, data analysis, and investigations of hematopoietic cells and cells from other tissues. The interactions among ABO, Lewis, and secretor histo-blood group systems are explored to resolve cases of hematopoietic chimera. Here we report a rare case of hematopoietic chimera where twins present a mixed field reaction in the ABO, Rh, and Kidd red blood cell phenotyping. Using red blood cells separated from the mixed field as well as molecular approaches and investigations of family members, we identify inconsistent genotypes with the Mendelian inheritance pattern when comparing the peripheral blood with the buccal epithelium of the male twin and his twin sister. Analysis of the ABO, Lewis, and secretor phenotypes, and genomic DNA from buccal epithelium showed the genotypes ABO*A1.01/ABO*B.01 and FUT2*01N.02/FUT2*01N.02 in the male twin and the genotypes ABO*O.01.01/ABO*O.01.02 and FUT2*01/FUT2*01 in the female twin. The results of the HLA-DRB1 genotyping showed inconsistency between the male and his twin sister. We conclude that the serological analyses combined with molecular approaches used in this study are good tools to resolve cases of hematopoietic chimera. (C) 2019 S. Karger AG, BaselMed Sch Sao Jose do Rio Preto FAMERP, Dept Mol Biol, Ave Brigadeiro Faria Lima 5416, BR-15090000 Sao Jose Do Rio Preto, SP, BrazilInst Biociencias Letras Ciencias Exatas IBILCE, Dept Biol, Campus Sao Jose do Rio Preto, Sao Jose Do Rio Preto, BrazilHosp Naval Marcilio Dias, Dept Special Serv, Hemotherapy Serv, Rio De Janeiro, BrazilMed Sch Sao Jose do Rio Preto FAMERP, Dept Med, Sao Jose Do Rio Preto, BrazilInst Biociencias Letras Ciencias Exatas IBILCE, Dept Biol, Campus Sao Jose do Rio Preto, Sao Jose Do Rio Preto, BrazilKargerMed Sch Sao Jose do Rio Preto FAMERPUniversidade Estadual Paulista (Unesp)Hosp Naval Marcilio DiasUniversidade de São Paulo (USP)Miola, Marcos P.Lopes, Alessandro G. [UNESP]Silva, Alessandra P.Gomes, Edney G. C.Machado, Leticia A. F.Veloso, Wanessa A.Costa, Carlos A.Fachini, Roberta M.Ricci Junior, OctavioBrandao de Mattos, Cinara C.Mattos, Luiz Carlos de2020-12-10T19:41:03Z2020-12-10T19:41:03Z2019-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article276-281http://dx.doi.org/10.1159/000495583Transfusion Medicine And Hemotherapy. Basel: Karger, v. 46, n. 4, p. 276-281, 2019.1660-3796http://hdl.handle.net/11449/19632510.1159/000495583WOS:000496287600008Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTransfusion Medicine And Hemotherapyinfo:eu-repo/semantics/openAccess2021-10-23T07:07:28Zoai:repositorio.unesp.br:11449/196325Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:52:13.045214Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Hematopoietic Chimera in a Male Blood Donor and His Dizygotic Twin Sister
title Hematopoietic Chimera in a Male Blood Donor and His Dizygotic Twin Sister
spellingShingle Hematopoietic Chimera in a Male Blood Donor and His Dizygotic Twin Sister
Miola, Marcos P.
Chimerism
Dizygotic twins
Blood groups
Phenotyping
Molecular analysis
title_short Hematopoietic Chimera in a Male Blood Donor and His Dizygotic Twin Sister
title_full Hematopoietic Chimera in a Male Blood Donor and His Dizygotic Twin Sister
title_fullStr Hematopoietic Chimera in a Male Blood Donor and His Dizygotic Twin Sister
title_full_unstemmed Hematopoietic Chimera in a Male Blood Donor and His Dizygotic Twin Sister
title_sort Hematopoietic Chimera in a Male Blood Donor and His Dizygotic Twin Sister
author Miola, Marcos P.
author_facet Miola, Marcos P.
Lopes, Alessandro G. [UNESP]
Silva, Alessandra P.
Gomes, Edney G. C.
Machado, Leticia A. F.
Veloso, Wanessa A.
Costa, Carlos A.
Fachini, Roberta M.
Ricci Junior, Octavio
Brandao de Mattos, Cinara C.
Mattos, Luiz Carlos de
author_role author
author2 Lopes, Alessandro G. [UNESP]
Silva, Alessandra P.
Gomes, Edney G. C.
Machado, Leticia A. F.
Veloso, Wanessa A.
Costa, Carlos A.
Fachini, Roberta M.
Ricci Junior, Octavio
Brandao de Mattos, Cinara C.
Mattos, Luiz Carlos de
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Med Sch Sao Jose do Rio Preto FAMERP
Universidade Estadual Paulista (Unesp)
Hosp Naval Marcilio Dias
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Miola, Marcos P.
Lopes, Alessandro G. [UNESP]
Silva, Alessandra P.
Gomes, Edney G. C.
Machado, Leticia A. F.
Veloso, Wanessa A.
Costa, Carlos A.
Fachini, Roberta M.
Ricci Junior, Octavio
Brandao de Mattos, Cinara C.
Mattos, Luiz Carlos de
dc.subject.por.fl_str_mv Chimerism
Dizygotic twins
Blood groups
Phenotyping
Molecular analysis
topic Chimerism
Dizygotic twins
Blood groups
Phenotyping
Molecular analysis
description Twin hematopoietic chimera in humans is a phenomenon that was discovered accidentally and the prevalence of which remains unclear. The resolution of chimera cases requires studying family medical records, data analysis, and investigations of hematopoietic cells and cells from other tissues. The interactions among ABO, Lewis, and secretor histo-blood group systems are explored to resolve cases of hematopoietic chimera. Here we report a rare case of hematopoietic chimera where twins present a mixed field reaction in the ABO, Rh, and Kidd red blood cell phenotyping. Using red blood cells separated from the mixed field as well as molecular approaches and investigations of family members, we identify inconsistent genotypes with the Mendelian inheritance pattern when comparing the peripheral blood with the buccal epithelium of the male twin and his twin sister. Analysis of the ABO, Lewis, and secretor phenotypes, and genomic DNA from buccal epithelium showed the genotypes ABO*A1.01/ABO*B.01 and FUT2*01N.02/FUT2*01N.02 in the male twin and the genotypes ABO*O.01.01/ABO*O.01.02 and FUT2*01/FUT2*01 in the female twin. The results of the HLA-DRB1 genotyping showed inconsistency between the male and his twin sister. We conclude that the serological analyses combined with molecular approaches used in this study are good tools to resolve cases of hematopoietic chimera. (C) 2019 S. Karger AG, Basel
publishDate 2019
dc.date.none.fl_str_mv 2019-08-01
2020-12-10T19:41:03Z
2020-12-10T19:41:03Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1159/000495583
Transfusion Medicine And Hemotherapy. Basel: Karger, v. 46, n. 4, p. 276-281, 2019.
1660-3796
http://hdl.handle.net/11449/196325
10.1159/000495583
WOS:000496287600008
url http://dx.doi.org/10.1159/000495583
http://hdl.handle.net/11449/196325
identifier_str_mv Transfusion Medicine And Hemotherapy. Basel: Karger, v. 46, n. 4, p. 276-281, 2019.
1660-3796
10.1159/000495583
WOS:000496287600008
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Transfusion Medicine And Hemotherapy
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 276-281
dc.publisher.none.fl_str_mv Karger
publisher.none.fl_str_mv Karger
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129132231393280

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