Effects of hypertonic solutions on two species of human intestinal parasites during fecal examination

Detalhes bibliográficos
Autor(a) principal: Stefano, Vitoria C
Data de Publicação: 2023
Outros Autores: Loiola, Saulo Hn, Dos Santos, Bianca M, Soares, Felipe A, Rosa, Stefany L, Suzuki, Celso Tn, Bresciani, Katia Ds [UNESP], Sabadini, Edvaldo, Falcao, Alexandre X, Gomes, Jancarlo F
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.2217/fmb-2022-0055
http://hdl.handle.net/11449/247145
Resumo: Aim: Ova and parasite examination by flotation requires hypertonic solutions, which can damage the egg and cyst membranes, leading to false negatives. The authors investigated the harmful effects of ZnSO4 and C12H22O11 solutions on the ova and parasite examination. Materials methods: The authors processed samples using the Three Fecal Test technique. Aliquots were floated in different pH levels, temperatures and solution densities. Results: Densities above 1.12 g/ml led structures to collapse after 6-10 min. pH neutralization of the ZnSO4 solution did not prevent the parasites from changing. Conclusion: All structures were altered when standard methods were performed. To delay collapse, the parasite floating under 5 °C is highly desirable.
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spelling Effects of hypertonic solutions on two species of human intestinal parasites during fecal examinationC12H22O11hypertonic solutionparasitic infectionsparasitology examinationZnSO4Aim: Ova and parasite examination by flotation requires hypertonic solutions, which can damage the egg and cyst membranes, leading to false negatives. The authors investigated the harmful effects of ZnSO4 and C12H22O11 solutions on the ova and parasite examination. Materials methods: The authors processed samples using the Three Fecal Test technique. Aliquots were floated in different pH levels, temperatures and solution densities. Results: Densities above 1.12 g/ml led structures to collapse after 6-10 min. pH neutralization of the ZnSO4 solution did not prevent the parasites from changing. Conclusion: All structures were altered when standard methods were performed. To delay collapse, the parasite floating under 5 °C is highly desirable.Laboratory of Image Data Science Institute of Computing University of Campinas, 573, IC-3, 5, Saturnino de Brito St., Room 364, CampinasSchool of Medical Sciences University of Campinas, 126, Tessalia Vieira de Camargo St., CampinasSchool of Veterinary Medicine Sao Paulo State University, Aracatuba, 793, Clovis Pestana St., AracatubaInstitute of Chemistry University of Campinas, 126, Josue de Castro St., CampinasSchool of Veterinary Medicine Sao Paulo State University, Aracatuba, 793, Clovis Pestana St., AracatubaUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)Stefano, Vitoria CLoiola, Saulo HnDos Santos, Bianca MSoares, Felipe ARosa, Stefany LSuzuki, Celso TnBresciani, Katia Ds [UNESP]Sabadini, EdvaldoFalcao, Alexandre XGomes, Jancarlo F2023-07-29T13:07:31Z2023-07-29T13:07:31Z2023-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article197-203http://dx.doi.org/10.2217/fmb-2022-0055Future Microbiology, v. 18, n. 3, p. 197-203, 2023.1746-09211746-0913http://hdl.handle.net/11449/24714510.2217/fmb-2022-00552-s2.0-85152152497Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFuture Microbiologyinfo:eu-repo/semantics/openAccess2023-07-29T13:07:31Zoai:repositorio.unesp.br:11449/247145Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:04:41.238705Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of hypertonic solutions on two species of human intestinal parasites during fecal examination
title Effects of hypertonic solutions on two species of human intestinal parasites during fecal examination
spellingShingle Effects of hypertonic solutions on two species of human intestinal parasites during fecal examination
Stefano, Vitoria C
C12H22O11
hypertonic solution
parasitic infections
parasitology examination
ZnSO4
title_short Effects of hypertonic solutions on two species of human intestinal parasites during fecal examination
title_full Effects of hypertonic solutions on two species of human intestinal parasites during fecal examination
title_fullStr Effects of hypertonic solutions on two species of human intestinal parasites during fecal examination
title_full_unstemmed Effects of hypertonic solutions on two species of human intestinal parasites during fecal examination
title_sort Effects of hypertonic solutions on two species of human intestinal parasites during fecal examination
author Stefano, Vitoria C
author_facet Stefano, Vitoria C
Loiola, Saulo Hn
Dos Santos, Bianca M
Soares, Felipe A
Rosa, Stefany L
Suzuki, Celso Tn
Bresciani, Katia Ds [UNESP]
Sabadini, Edvaldo
Falcao, Alexandre X
Gomes, Jancarlo F
author_role author
author2 Loiola, Saulo Hn
Dos Santos, Bianca M
Soares, Felipe A
Rosa, Stefany L
Suzuki, Celso Tn
Bresciani, Katia Ds [UNESP]
Sabadini, Edvaldo
Falcao, Alexandre X
Gomes, Jancarlo F
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Stefano, Vitoria C
Loiola, Saulo Hn
Dos Santos, Bianca M
Soares, Felipe A
Rosa, Stefany L
Suzuki, Celso Tn
Bresciani, Katia Ds [UNESP]
Sabadini, Edvaldo
Falcao, Alexandre X
Gomes, Jancarlo F
dc.subject.por.fl_str_mv C12H22O11
hypertonic solution
parasitic infections
parasitology examination
ZnSO4
topic C12H22O11
hypertonic solution
parasitic infections
parasitology examination
ZnSO4
description Aim: Ova and parasite examination by flotation requires hypertonic solutions, which can damage the egg and cyst membranes, leading to false negatives. The authors investigated the harmful effects of ZnSO4 and C12H22O11 solutions on the ova and parasite examination. Materials methods: The authors processed samples using the Three Fecal Test technique. Aliquots were floated in different pH levels, temperatures and solution densities. Results: Densities above 1.12 g/ml led structures to collapse after 6-10 min. pH neutralization of the ZnSO4 solution did not prevent the parasites from changing. Conclusion: All structures were altered when standard methods were performed. To delay collapse, the parasite floating under 5 °C is highly desirable.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:07:31Z
2023-07-29T13:07:31Z
2023-02-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.2217/fmb-2022-0055
Future Microbiology, v. 18, n. 3, p. 197-203, 2023.
1746-0921
1746-0913
http://hdl.handle.net/11449/247145
10.2217/fmb-2022-0055
2-s2.0-85152152497
url http://dx.doi.org/10.2217/fmb-2022-0055
http://hdl.handle.net/11449/247145
identifier_str_mv Future Microbiology, v. 18, n. 3, p. 197-203, 2023.
1746-0921
1746-0913
10.2217/fmb-2022-0055
2-s2.0-85152152497
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Future Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 197-203
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128455939719168