Determinação precoce do sexo fetal pela análise do DNA no plasma materno
Autor(a) principal: | |
---|---|
Data de Publicação: | 2006 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-72032006000300009 http://hdl.handle.net/11449/69102 |
Resumo: | Purpose: to verify the viability of early diagnosis of fetal gender in maternal plasma by the real-time polymerase chain reaction (real-time PCR) starting at the 5th week of pregnancy. Methods: peripheral blood was collected from pregnant women with single fetus starting at the 5th week of gestation. After centrifugation, 0.4 mL plasma was separated for fetal DNA extraction. The DNA was analyzed in duplicate by real-time PCR for two genomic regions, one of the Y chromosome and the other common to both sexes, through the TaqMan® method, which uses a pair of primers and a fluorescent probe. Patients who aborted were excluded. Results: a total of 79 determinations of fetal DNA in maternal plasma were performed in 52 pregnant women. The results of the determinations were compared to fetal gender after delivery. Accuracy according to gestational age was 92.6% (25 of 27 cases) at 5 weeks with 87% sensitivity, and 95.6% (22 of 23 cases) at 6 weeks with 92% sensitivity. Starting at the 7th week of pregnancy, accuracy was 100% (29 of 29 cases). Specificity was 100% regardless of gestational age. Conclusion: real-time PCR for the detection of fetal gender in maternal plasma starting at the 5th week of gestation has good sensitivity and excellent specificity. There was agreement of the results in 100% of the cases in which male gender was diagnosed, regardless of gestational age, and from the 7th week of gestation for female gender diagnosis. |
id |
UNSP_e433dff91e9b83015924653c6da38bc8 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/69102 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Determinação precoce do sexo fetal pela análise do DNA no plasma maternoEarly determination of gender by analysis of fetal DNA in maternal plasmaGestational agePolymerase chain reaction/methodsPre-natal diagnosisSex determination (Analysis)Purpose: to verify the viability of early diagnosis of fetal gender in maternal plasma by the real-time polymerase chain reaction (real-time PCR) starting at the 5th week of pregnancy. Methods: peripheral blood was collected from pregnant women with single fetus starting at the 5th week of gestation. After centrifugation, 0.4 mL plasma was separated for fetal DNA extraction. The DNA was analyzed in duplicate by real-time PCR for two genomic regions, one of the Y chromosome and the other common to both sexes, through the TaqMan® method, which uses a pair of primers and a fluorescent probe. Patients who aborted were excluded. Results: a total of 79 determinations of fetal DNA in maternal plasma were performed in 52 pregnant women. The results of the determinations were compared to fetal gender after delivery. Accuracy according to gestational age was 92.6% (25 of 27 cases) at 5 weeks with 87% sensitivity, and 95.6% (22 of 23 cases) at 6 weeks with 92% sensitivity. Starting at the 7th week of pregnancy, accuracy was 100% (29 of 29 cases). Specificity was 100% regardless of gestational age. Conclusion: real-time PCR for the detection of fetal gender in maternal plasma starting at the 5th week of gestation has good sensitivity and excellent specificity. There was agreement of the results in 100% of the cases in which male gender was diagnosed, regardless of gestational age, and from the 7th week of gestation for female gender diagnosis.Centro Paulista de Diagnóstico e Pesquisa - CPDP, Ribeirão Preto (SP)Programa de Pós-Graduação em Ginecologia, Obstetrícia e Mastologia Faculdade de Medicina de Botucatu Universidade Estadual Paulista Julio Mesquita Filho - UNESP, Botucatu (SP)RDO Diagnósticos Médicos, São Paulo (SP)Bióloga do CPDP Centro Paulista de Diagnóstico e Pesquisa, Ribeirão Preto (SP)Bioquímica do CPDP Centro Paulista de Diagnóstico e Pesquisa, Ribeirão Preto (SP)Centro de Reprodução Humana - Prof. Franco Junior, Ribeirão Preto (SP), Av. Professor João Fiusa, 689, 14025-310 - Ribeirao Preto - SPPrograma de Pós-Graduação em Ginecologia, Obstetrícia e Mastologia Faculdade de Medicina de Botucatu Universidade Estadual Paulista Julio Mesquita Filho - UNESP, Botucatu (SP)Centro Paulista de Diagnóstico e Pesquisa - CPDPUniversidade Estadual Paulista (Unesp)RDO Diagnósticos MédicosCentro Paulista de Diagnóstico e PesquisaCentro de Reprodução Humana - Prof. Franco JuniorMartinhago, Ciro Dresch [UNESP]De Oliveira, Ricardo ManoelCanas, Maria Do Carmo TomitãoVagnini, Laura DinizOliveira, João Batista AlcantaraPetersen, Claudia GuilherminoFranco Junior, Jose Gonçalves [UNESP]2014-05-27T11:21:58Z2014-05-27T11:21:58Z2006-09-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article190-194application/pdfhttp://dx.doi.org/10.1590/S0100-72032006000300009Revista Brasileira de Ginecologia e Obstetricia, v. 28, n. 3, p. 190-194, 2006.0100-7203http://hdl.handle.net/11449/6910210.1590/S0100-72032006000300009S0100-720320060003000092-s2.0-337488895012-s2.0-33748889501.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista Brasileira de Ginecologia e Obstetrícia0,292info:eu-repo/semantics/openAccess2024-08-16T14:06:29Zoai:repositorio.unesp.br:11449/69102Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T14:06:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Determinação precoce do sexo fetal pela análise do DNA no plasma materno Early determination of gender by analysis of fetal DNA in maternal plasma |
title |
Determinação precoce do sexo fetal pela análise do DNA no plasma materno |
spellingShingle |
Determinação precoce do sexo fetal pela análise do DNA no plasma materno Martinhago, Ciro Dresch [UNESP] Gestational age Polymerase chain reaction/methods Pre-natal diagnosis Sex determination (Analysis) |
title_short |
Determinação precoce do sexo fetal pela análise do DNA no plasma materno |
title_full |
Determinação precoce do sexo fetal pela análise do DNA no plasma materno |
title_fullStr |
Determinação precoce do sexo fetal pela análise do DNA no plasma materno |
title_full_unstemmed |
Determinação precoce do sexo fetal pela análise do DNA no plasma materno |
title_sort |
Determinação precoce do sexo fetal pela análise do DNA no plasma materno |
author |
Martinhago, Ciro Dresch [UNESP] |
author_facet |
Martinhago, Ciro Dresch [UNESP] De Oliveira, Ricardo Manoel Canas, Maria Do Carmo Tomitão Vagnini, Laura Diniz Oliveira, João Batista Alcantara Petersen, Claudia Guilhermino Franco Junior, Jose Gonçalves [UNESP] |
author_role |
author |
author2 |
De Oliveira, Ricardo Manoel Canas, Maria Do Carmo Tomitão Vagnini, Laura Diniz Oliveira, João Batista Alcantara Petersen, Claudia Guilhermino Franco Junior, Jose Gonçalves [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Centro Paulista de Diagnóstico e Pesquisa - CPDP Universidade Estadual Paulista (Unesp) RDO Diagnósticos Médicos Centro Paulista de Diagnóstico e Pesquisa Centro de Reprodução Humana - Prof. Franco Junior |
dc.contributor.author.fl_str_mv |
Martinhago, Ciro Dresch [UNESP] De Oliveira, Ricardo Manoel Canas, Maria Do Carmo Tomitão Vagnini, Laura Diniz Oliveira, João Batista Alcantara Petersen, Claudia Guilhermino Franco Junior, Jose Gonçalves [UNESP] |
dc.subject.por.fl_str_mv |
Gestational age Polymerase chain reaction/methods Pre-natal diagnosis Sex determination (Analysis) |
topic |
Gestational age Polymerase chain reaction/methods Pre-natal diagnosis Sex determination (Analysis) |
description |
Purpose: to verify the viability of early diagnosis of fetal gender in maternal plasma by the real-time polymerase chain reaction (real-time PCR) starting at the 5th week of pregnancy. Methods: peripheral blood was collected from pregnant women with single fetus starting at the 5th week of gestation. After centrifugation, 0.4 mL plasma was separated for fetal DNA extraction. The DNA was analyzed in duplicate by real-time PCR for two genomic regions, one of the Y chromosome and the other common to both sexes, through the TaqMan® method, which uses a pair of primers and a fluorescent probe. Patients who aborted were excluded. Results: a total of 79 determinations of fetal DNA in maternal plasma were performed in 52 pregnant women. The results of the determinations were compared to fetal gender after delivery. Accuracy according to gestational age was 92.6% (25 of 27 cases) at 5 weeks with 87% sensitivity, and 95.6% (22 of 23 cases) at 6 weeks with 92% sensitivity. Starting at the 7th week of pregnancy, accuracy was 100% (29 of 29 cases). Specificity was 100% regardless of gestational age. Conclusion: real-time PCR for the detection of fetal gender in maternal plasma starting at the 5th week of gestation has good sensitivity and excellent specificity. There was agreement of the results in 100% of the cases in which male gender was diagnosed, regardless of gestational age, and from the 7th week of gestation for female gender diagnosis. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-09-27 2014-05-27T11:21:58Z 2014-05-27T11:21:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-72032006000300009 Revista Brasileira de Ginecologia e Obstetricia, v. 28, n. 3, p. 190-194, 2006. 0100-7203 http://hdl.handle.net/11449/69102 10.1590/S0100-72032006000300009 S0100-72032006000300009 2-s2.0-33748889501 2-s2.0-33748889501.pdf |
url |
http://dx.doi.org/10.1590/S0100-72032006000300009 http://hdl.handle.net/11449/69102 |
identifier_str_mv |
Revista Brasileira de Ginecologia e Obstetricia, v. 28, n. 3, p. 190-194, 2006. 0100-7203 10.1590/S0100-72032006000300009 S0100-72032006000300009 2-s2.0-33748889501 2-s2.0-33748889501.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Revista Brasileira de Ginecologia e Obstetrícia 0,292 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
190-194 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128111577923584 |