Viabilidad de espermatozoides de ovinos en diferentes sistemas de refrigeración

• Autor(a) principal: Moscardini, Murillo Mansano
• Data de Publicação: 2014
• Outros Autores: Scott, Caroline, Moura, Diego Souza, Lourenço, Tarcísio Torre, Aristizábal, Viviane Helena Vallejo [UNESP], Souza, Fabiana Ferreira de [UNESP]
• Tipo de documento: Artigo
• Idioma: spa
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.4322/rbcv.2014.035; http://hdl.handle.net/11449/141308
• topic: Cryopreservation; Ram; Spermatozoa; Carnero; Crio-preservación; Espermatozoide

The aim of this study was to evaluate the ovine sperm quality colled in thermal boxs. Six ejaculates from 2 rams were collected using artificial vagina. Samples were analyzed to volume, mass movement, motility, vigor, sperm cell concentration, hypo-osmotic swelling test and supravital stain. Ejaculates were divided into 2 aliquots and diluted in 0.9% NaCl or in the extender (Botubov®, Botupharma, Botucatu, SP, Brazil), and was kept at room temperature, refrigerator (5°C), Botubox® (15°C, Botupharma, Botucatu, SP, Brazil), Botutainer® (5ºC, Botupharma, Botucatu, SP, Brazil) and MaxSemen® (15°C, EHG Agrofarma, Campinas, SP, Brazil). All samples were analyzed each 24 hours to the same parameters. According to the results the samples diluted and cooled in extender preserved sperm viability until 48 hours in thermal boxes and in refrigerator; and the samples extended preserved in environment maintained motility 30% up to 72 hours. Samples diluted in 0.9% saline retained motility around 30% until 24 hours of cooling. Based on the results it is concluded that the three boxes may be used for transport of ram semen, diluted and refrigerated for a period of 24 to 48 hours.