Cryopreservation of dog spermatozoa: Effect of bovine serum albumin on acrosomal integrity and pregnancy rates after artificial insemination
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://ojs.c3sl.ufpr.br/ojs2/index.php/veterinary/article/view/9909 http://hdl.handle.net/11449/70229 |
Resumo: | The objective of the present study was to compare the in vitro and in vivo profile of frozen dog semen with Tris-bovine serum albumin (TB) and Tris-egg yolk (TE) extenders. Twenty dogs were used as donors. Each dog was stimulated by penile massage and only the sperm-rich fraction was collected weekly until 40 ejaculates were obtained. After macroscopic and microscopic analysis, equal parts of each ejaculate were diluted with TB and TE by the one-step method at 37 °C. The semen was added to 0.5-mL French straws which presented normal characteristics before freezing and after thawing. Acrosomal integrity was evaluated by double Trypan blue-Giemsa staining, in which alive intact (LI), alive reacted (LR), dead intact (DI) and dead reacted (DR) spermatozoa, were identified by the time of thawing and up to 4 h of incubation at 39 °C, the TE being significantly superior to TB (P<0,01) in the LI and LR variables. The TB being significantly superior to TE (P<0,01) in the DR variable. Female dogs in natural heat were submitted to artificial insemination, 20 receiving TE-semen and 20 receiving TB-semen with the Osiris probe (IMV, L'Aigle, France) and the numbers indicate that TE was significantly better than TB (P<0,01) to pregnancy rate and number of puppies/delivery. We concluded from this study, that TE was better than TB, because this, induced an eady acrossome reaction in dog's sperm. |
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Cryopreservation of dog spermatozoa: Effect of bovine serum albumin on acrosomal integrity and pregnancy rates after artificial inseminationBitchExtendersOestrusSemenBovinaeCanis familiarisOsirisThe objective of the present study was to compare the in vitro and in vivo profile of frozen dog semen with Tris-bovine serum albumin (TB) and Tris-egg yolk (TE) extenders. Twenty dogs were used as donors. Each dog was stimulated by penile massage and only the sperm-rich fraction was collected weekly until 40 ejaculates were obtained. After macroscopic and microscopic analysis, equal parts of each ejaculate were diluted with TB and TE by the one-step method at 37 °C. The semen was added to 0.5-mL French straws which presented normal characteristics before freezing and after thawing. Acrosomal integrity was evaluated by double Trypan blue-Giemsa staining, in which alive intact (LI), alive reacted (LR), dead intact (DI) and dead reacted (DR) spermatozoa, were identified by the time of thawing and up to 4 h of incubation at 39 °C, the TE being significantly superior to TB (P<0,01) in the LI and LR variables. The TB being significantly superior to TE (P<0,01) in the DR variable. Female dogs in natural heat were submitted to artificial insemination, 20 receiving TE-semen and 20 receiving TB-semen with the Osiris probe (IMV, L'Aigle, France) and the numbers indicate that TE was significantly better than TB (P<0,01) to pregnancy rate and number of puppies/delivery. We concluded from this study, that TE was better than TB, because this, induced an eady acrossome reaction in dog's sperm.Departamento de Medicina Veterinária UFPR, Rua Pioneiro 2153, 85950-000 Palotina, PRDepartamento de Medicina Veterinária Preventiva e Reprodução Animal UNESP, Jaboticabal, SPDepartamento de Medicina Veterinária UFPR, Curitiba, SPDepartamento de Medicina Veterinária PUCPR, Sao Jose dos Pinhais, PRDepartamento de Medicina Veterinária Preventiva e Reprodução Animal UNESP, Jaboticabal, SPUniversidade Federal do Paraná (UFPR)Universidade Estadual Paulista (Unesp)Pontifícia Universidade Católica do Paraná (PUCPR)Santos, I. WalterBinsfeld, L. C.Weiss, R. R.Kozicki, L. E.Rochadelli, R.Resende, M. V. [UNESP]Hossepian de Lima, V. F M [UNESP]2014-05-27T11:22:45Z2014-05-27T11:22:45Z2007-12-31info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article47-54application/pdfhttp://ojs.c3sl.ufpr.br/ojs2/index.php/veterinary/article/view/9909Archives of Veterinary Science, v. 12, n. 2, p. 47-54, 2007.1517-784Xhttp://hdl.handle.net/11449/702292-s2.0-373490172602-s2.0-37349017260.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Veterinary Scienceinfo:eu-repo/semantics/openAccess2024-06-06T18:10:12Zoai:repositorio.unesp.br:11449/70229Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-06T18:10:12Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cryopreservation of dog spermatozoa: Effect of bovine serum albumin on acrosomal integrity and pregnancy rates after artificial insemination |
title |
Cryopreservation of dog spermatozoa: Effect of bovine serum albumin on acrosomal integrity and pregnancy rates after artificial insemination |
spellingShingle |
Cryopreservation of dog spermatozoa: Effect of bovine serum albumin on acrosomal integrity and pregnancy rates after artificial insemination Santos, I. Walter Bitch Extenders Oestrus Semen Bovinae Canis familiaris Osiris |
title_short |
Cryopreservation of dog spermatozoa: Effect of bovine serum albumin on acrosomal integrity and pregnancy rates after artificial insemination |
title_full |
Cryopreservation of dog spermatozoa: Effect of bovine serum albumin on acrosomal integrity and pregnancy rates after artificial insemination |
title_fullStr |
Cryopreservation of dog spermatozoa: Effect of bovine serum albumin on acrosomal integrity and pregnancy rates after artificial insemination |
title_full_unstemmed |
Cryopreservation of dog spermatozoa: Effect of bovine serum albumin on acrosomal integrity and pregnancy rates after artificial insemination |
title_sort |
Cryopreservation of dog spermatozoa: Effect of bovine serum albumin on acrosomal integrity and pregnancy rates after artificial insemination |
author |
Santos, I. Walter |
author_facet |
Santos, I. Walter Binsfeld, L. C. Weiss, R. R. Kozicki, L. E. Rochadelli, R. Resende, M. V. [UNESP] Hossepian de Lima, V. F M [UNESP] |
author_role |
author |
author2 |
Binsfeld, L. C. Weiss, R. R. Kozicki, L. E. Rochadelli, R. Resende, M. V. [UNESP] Hossepian de Lima, V. F M [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Paraná (UFPR) Universidade Estadual Paulista (Unesp) Pontifícia Universidade Católica do Paraná (PUCPR) |
dc.contributor.author.fl_str_mv |
Santos, I. Walter Binsfeld, L. C. Weiss, R. R. Kozicki, L. E. Rochadelli, R. Resende, M. V. [UNESP] Hossepian de Lima, V. F M [UNESP] |
dc.subject.por.fl_str_mv |
Bitch Extenders Oestrus Semen Bovinae Canis familiaris Osiris |
topic |
Bitch Extenders Oestrus Semen Bovinae Canis familiaris Osiris |
description |
The objective of the present study was to compare the in vitro and in vivo profile of frozen dog semen with Tris-bovine serum albumin (TB) and Tris-egg yolk (TE) extenders. Twenty dogs were used as donors. Each dog was stimulated by penile massage and only the sperm-rich fraction was collected weekly until 40 ejaculates were obtained. After macroscopic and microscopic analysis, equal parts of each ejaculate were diluted with TB and TE by the one-step method at 37 °C. The semen was added to 0.5-mL French straws which presented normal characteristics before freezing and after thawing. Acrosomal integrity was evaluated by double Trypan blue-Giemsa staining, in which alive intact (LI), alive reacted (LR), dead intact (DI) and dead reacted (DR) spermatozoa, were identified by the time of thawing and up to 4 h of incubation at 39 °C, the TE being significantly superior to TB (P<0,01) in the LI and LR variables. The TB being significantly superior to TE (P<0,01) in the DR variable. Female dogs in natural heat were submitted to artificial insemination, 20 receiving TE-semen and 20 receiving TB-semen with the Osiris probe (IMV, L'Aigle, France) and the numbers indicate that TE was significantly better than TB (P<0,01) to pregnancy rate and number of puppies/delivery. We concluded from this study, that TE was better than TB, because this, induced an eady acrossome reaction in dog's sperm. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-12-31 2014-05-27T11:22:45Z 2014-05-27T11:22:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://ojs.c3sl.ufpr.br/ojs2/index.php/veterinary/article/view/9909 Archives of Veterinary Science, v. 12, n. 2, p. 47-54, 2007. 1517-784X http://hdl.handle.net/11449/70229 2-s2.0-37349017260 2-s2.0-37349017260.pdf |
url |
http://ojs.c3sl.ufpr.br/ojs2/index.php/veterinary/article/view/9909 http://hdl.handle.net/11449/70229 |
identifier_str_mv |
Archives of Veterinary Science, v. 12, n. 2, p. 47-54, 2007. 1517-784X 2-s2.0-37349017260 2-s2.0-37349017260.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Veterinary Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
47-54 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803650213172215808 |