A systems-wide comparison of red rice (Oryza longistaminata) tissues identifies rhizome specific genes and proteins that are targets for cultivated rice improvement
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1471-2229-14-46 http://hdl.handle.net/11449/227547 |
Resumo: | Background: The rhizome, the original stem of land plants, enables species to invade new territory and is a critical component of perenniality, especially in grasses. Red rice (Oryza longistaminata) is a perennial wild rice species with many valuable traits that could be used to improve cultivated rice cultivars, including rhizomatousness, disease resistance and drought tolerance. Despite these features, little is known about the molecular mechanisms that contribute to rhizome growth, development and function in this plant.Results: We used an integrated approach to compare the transcriptome, proteome and metabolome of the rhizome to other tissues of red rice. 116 Gb of transcriptome sequence was obtained from various tissues and used to identify rhizome-specific and preferentially expressed genes, including transcription factors and hormone metabolism and stress response-related genes. Proteomics and metabolomics approaches identified 41 proteins and more than 100 primary metabolites and plant hormones with rhizome preferential accumulation. Of particular interest was the identification of a large number of gene transcripts from Magnaportha oryzae, the fungus that causes rice blast disease in cultivated rice, even though the red rice plants showed no sign of disease.Conclusions: A significant set of genes, proteins and metabolites appear to be specifically or preferentially expressed in the rhizome of O. longistaminata. The presence of M. oryzae gene transcripts at a high level in apparently healthy plants suggests that red rice is resistant to this pathogen, and may be able to provide genes to cultivated rice that will enable resistance to rice blast disease. © 2014 He et al.; licensee BioMed Central Ltd. |
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A systems-wide comparison of red rice (Oryza longistaminata) tissues identifies rhizome specific genes and proteins that are targets for cultivated rice improvementDisease resistanceInvasive speciesMetabolomicsProteomicsRhizomeRiceRice blastTranscriptomicsBackground: The rhizome, the original stem of land plants, enables species to invade new territory and is a critical component of perenniality, especially in grasses. Red rice (Oryza longistaminata) is a perennial wild rice species with many valuable traits that could be used to improve cultivated rice cultivars, including rhizomatousness, disease resistance and drought tolerance. Despite these features, little is known about the molecular mechanisms that contribute to rhizome growth, development and function in this plant.Results: We used an integrated approach to compare the transcriptome, proteome and metabolome of the rhizome to other tissues of red rice. 116 Gb of transcriptome sequence was obtained from various tissues and used to identify rhizome-specific and preferentially expressed genes, including transcription factors and hormone metabolism and stress response-related genes. Proteomics and metabolomics approaches identified 41 proteins and more than 100 primary metabolites and plant hormones with rhizome preferential accumulation. Of particular interest was the identification of a large number of gene transcripts from Magnaportha oryzae, the fungus that causes rice blast disease in cultivated rice, even though the red rice plants showed no sign of disease.Conclusions: A significant set of genes, proteins and metabolites appear to be specifically or preferentially expressed in the rhizome of O. longistaminata. The presence of M. oryzae gene transcripts at a high level in apparently healthy plants suggests that red rice is resistant to this pathogen, and may be able to provide genes to cultivated rice that will enable resistance to rice blast disease. © 2014 He et al.; licensee BioMed Central Ltd.National Science FoundationInstitute of Biological Chemistry Washington State University, PO Box 646340, Pullman, WA 99164Department of Biochemistry and Interdisciplinary Plant Group University of Missouri Christopher S. Bond Life Sciences Center, Columbia, MO 65211BIO5 Institute The University of Arizona, Tucson, AZ 85721National Center for Genome Resources, Santa Fe, NM 87505Departamento de Tecnologia Universidade Estadual Paulista, Jaboticabal, SP 14884-900Departamento de Tecnologia Universidade Estadual Paulista, Jaboticabal, SP 14884-900National Science Foundation: IOS-1044821Washington State UniversityChristopher S. Bond Life Sciences CenterThe University of ArizonaNational Center for Genome ResourcesUniversidade Estadual Paulista (UNESP)He, RuifengSalvato, Fernanda [UNESP]Park, Jeong-JinKim, Min-JeongNelson, WilliamBalbuena, Tiago S. [UNESP]Willer, MarkCrow, John A.May, Greg D.Soderlund, Carol A.Thelen, Jay J.Gang, David R.2022-04-29T07:13:51Z2022-04-29T07:13:51Z2014-02-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1186/1471-2229-14-46BMC Plant Biology, v. 14, n. 1, 2014.1471-2229http://hdl.handle.net/11449/22754710.1186/1471-2229-14-462-s2.0-84893701507Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Plant Biologyinfo:eu-repo/semantics/openAccess2022-04-29T07:13:51Zoai:repositorio.unesp.br:11449/227547Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T07:13:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A systems-wide comparison of red rice (Oryza longistaminata) tissues identifies rhizome specific genes and proteins that are targets for cultivated rice improvement |
title |
A systems-wide comparison of red rice (Oryza longistaminata) tissues identifies rhizome specific genes and proteins that are targets for cultivated rice improvement |
spellingShingle |
A systems-wide comparison of red rice (Oryza longistaminata) tissues identifies rhizome specific genes and proteins that are targets for cultivated rice improvement He, Ruifeng Disease resistance Invasive species Metabolomics Proteomics Rhizome Rice Rice blast Transcriptomics |
title_short |
A systems-wide comparison of red rice (Oryza longistaminata) tissues identifies rhizome specific genes and proteins that are targets for cultivated rice improvement |
title_full |
A systems-wide comparison of red rice (Oryza longistaminata) tissues identifies rhizome specific genes and proteins that are targets for cultivated rice improvement |
title_fullStr |
A systems-wide comparison of red rice (Oryza longistaminata) tissues identifies rhizome specific genes and proteins that are targets for cultivated rice improvement |
title_full_unstemmed |
A systems-wide comparison of red rice (Oryza longistaminata) tissues identifies rhizome specific genes and proteins that are targets for cultivated rice improvement |
title_sort |
A systems-wide comparison of red rice (Oryza longistaminata) tissues identifies rhizome specific genes and proteins that are targets for cultivated rice improvement |
author |
He, Ruifeng |
author_facet |
He, Ruifeng Salvato, Fernanda [UNESP] Park, Jeong-Jin Kim, Min-Jeong Nelson, William Balbuena, Tiago S. [UNESP] Willer, Mark Crow, John A. May, Greg D. Soderlund, Carol A. Thelen, Jay J. Gang, David R. |
author_role |
author |
author2 |
Salvato, Fernanda [UNESP] Park, Jeong-Jin Kim, Min-Jeong Nelson, William Balbuena, Tiago S. [UNESP] Willer, Mark Crow, John A. May, Greg D. Soderlund, Carol A. Thelen, Jay J. Gang, David R. |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Washington State University Christopher S. Bond Life Sciences Center The University of Arizona National Center for Genome Resources Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
He, Ruifeng Salvato, Fernanda [UNESP] Park, Jeong-Jin Kim, Min-Jeong Nelson, William Balbuena, Tiago S. [UNESP] Willer, Mark Crow, John A. May, Greg D. Soderlund, Carol A. Thelen, Jay J. Gang, David R. |
dc.subject.por.fl_str_mv |
Disease resistance Invasive species Metabolomics Proteomics Rhizome Rice Rice blast Transcriptomics |
topic |
Disease resistance Invasive species Metabolomics Proteomics Rhizome Rice Rice blast Transcriptomics |
description |
Background: The rhizome, the original stem of land plants, enables species to invade new territory and is a critical component of perenniality, especially in grasses. Red rice (Oryza longistaminata) is a perennial wild rice species with many valuable traits that could be used to improve cultivated rice cultivars, including rhizomatousness, disease resistance and drought tolerance. Despite these features, little is known about the molecular mechanisms that contribute to rhizome growth, development and function in this plant.Results: We used an integrated approach to compare the transcriptome, proteome and metabolome of the rhizome to other tissues of red rice. 116 Gb of transcriptome sequence was obtained from various tissues and used to identify rhizome-specific and preferentially expressed genes, including transcription factors and hormone metabolism and stress response-related genes. Proteomics and metabolomics approaches identified 41 proteins and more than 100 primary metabolites and plant hormones with rhizome preferential accumulation. Of particular interest was the identification of a large number of gene transcripts from Magnaportha oryzae, the fungus that causes rice blast disease in cultivated rice, even though the red rice plants showed no sign of disease.Conclusions: A significant set of genes, proteins and metabolites appear to be specifically or preferentially expressed in the rhizome of O. longistaminata. The presence of M. oryzae gene transcripts at a high level in apparently healthy plants suggests that red rice is resistant to this pathogen, and may be able to provide genes to cultivated rice that will enable resistance to rice blast disease. © 2014 He et al.; licensee BioMed Central Ltd. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-12 2022-04-29T07:13:51Z 2022-04-29T07:13:51Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1471-2229-14-46 BMC Plant Biology, v. 14, n. 1, 2014. 1471-2229 http://hdl.handle.net/11449/227547 10.1186/1471-2229-14-46 2-s2.0-84893701507 |
url |
http://dx.doi.org/10.1186/1471-2229-14-46 http://hdl.handle.net/11449/227547 |
identifier_str_mv |
BMC Plant Biology, v. 14, n. 1, 2014. 1471-2229 10.1186/1471-2229-14-46 2-s2.0-84893701507 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Plant Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965148706766848 |