Anti-Trichophyton Activity of Protocatechuates and Their Synergism with Fluconazole

Detalhes bibliográficos
Autor(a) principal: Soares, Luciana Arantes [UNESP]
Data de Publicação: 2014
Outros Autores: Gullo, Fernanda Patricia [UNESP], Orlandi Sardi, Janaina de Cassia [UNESP], Pitangui, Nayla de Souza [UNESP], Costa-Orlandi, Caroline Barcelos [UNESP], Sangalli-Leite, Fernanda [UNESP], Scorzoni, Liliana [UNESP], Regasini, Luis Octavio [UNESP], Petronio, Maicon Segalla [UNESP], Souza, Patricia Fernanda [UNESP], Siqueira Silva, Dulce Helena [UNESP], Mendes-Giannini, Maria José Soares [UNESP], Fusco-Almeida, Ana Marisa [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1155/2014/957860
http://hdl.handle.net/11449/113431
Resumo: Dermatophytosis and superficial mycosis are a major global public health problem that affects 20-25% of the world's population. The increase in fungal resistance to the commercially available antifungal agents, in conjunction with the limited spectrum of action of such drugs, emphasises the need to develop new antifungal agents. Natural products are attractive prototypes for antifungal agents due to their broad spectrum of biological activities. This study aimed to verify the antifungal activity of protocatechuic acid, 3,4-diacetoxybenzoic, and fourteen alkyl protocatechuates (3,4-dihydroxybenzoates) against Trichophyton rubrum and Trichophyton mentagrophytes and to further assess their activities when combined with fluconazole. Susceptibility and synergism assays were conducted as described in M38-A2 (CLSI), with modifications. Three strains of Trichophyton rubrum and three strains of Trichophyton mentagrophytes were used in this work. The pentyl, hexyl, heptyl, octyl, nonyl, and decyl protocatechuates showed great fungicidal effects, with minimum inhibitory concentration (MIC) values ranging from 0.97 to 7.8 mg/L. Heptyl showed a synergistic activity (FIC index = 0.49), reducing the MIC of fluconazole by fourfold. All substances tested were safe, especially the hexyl, heptyl, octyl, and nonyl compounds, all of which showed a high selectivity index, particularly in combination with fluconazole. These ester associations with fluconazole may represent a promising source of prototypes in the search for anti-Trichophyton therapeutic agents.
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spelling Anti-Trichophyton Activity of Protocatechuates and Their Synergism with FluconazoleDermatophytosis and superficial mycosis are a major global public health problem that affects 20-25% of the world's population. The increase in fungal resistance to the commercially available antifungal agents, in conjunction with the limited spectrum of action of such drugs, emphasises the need to develop new antifungal agents. Natural products are attractive prototypes for antifungal agents due to their broad spectrum of biological activities. This study aimed to verify the antifungal activity of protocatechuic acid, 3,4-diacetoxybenzoic, and fourteen alkyl protocatechuates (3,4-dihydroxybenzoates) against Trichophyton rubrum and Trichophyton mentagrophytes and to further assess their activities when combined with fluconazole. Susceptibility and synergism assays were conducted as described in M38-A2 (CLSI), with modifications. Three strains of Trichophyton rubrum and three strains of Trichophyton mentagrophytes were used in this work. The pentyl, hexyl, heptyl, octyl, nonyl, and decyl protocatechuates showed great fungicidal effects, with minimum inhibitory concentration (MIC) values ranging from 0.97 to 7.8 mg/L. Heptyl showed a synergistic activity (FIC index = 0.49), reducing the MIC of fluconazole by fourfold. All substances tested were safe, especially the hexyl, heptyl, octyl, and nonyl compounds, all of which showed a high selectivity index, particularly in combination with fluconazole. These ester associations with fluconazole may represent a promising source of prototypes in the search for anti-Trichophyton therapeutic agents.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)PADC (Support Program for Scientific, Faculty of Pharmaceutical Sciences, UNESP)UNESP, Fac Pharmaceut Sci, Dept Clin Anal, Lab Clin Mycol, BR-14801902 Araraquara, SP, BrazilUNESP, Inst Chem, BR-14800900 Araraquara, SP, BrazilUniv Estadual Paulista UNESP, Dept Clin Mycol, Fac Pharmaceut Sci, BR-14801902 Araraquara, SP, BrazilUNESP, Fac Pharmaceut Sci, Dept Clin Anal, Lab Clin Mycol, BR-14801902 Araraquara, SP, BrazilUNESP, Inst Chem, BR-14800900 Araraquara, SP, BrazilUniv Estadual Paulista UNESP, Dept Clin Mycol, Fac Pharmaceut Sci, BR-14801902 Araraquara, SP, BrazilHindawi Publishing CorporationUniversidade Estadual Paulista (Unesp)Soares, Luciana Arantes [UNESP]Gullo, Fernanda Patricia [UNESP]Orlandi Sardi, Janaina de Cassia [UNESP]Pitangui, Nayla de Souza [UNESP]Costa-Orlandi, Caroline Barcelos [UNESP]Sangalli-Leite, Fernanda [UNESP]Scorzoni, Liliana [UNESP]Regasini, Luis Octavio [UNESP]Petronio, Maicon Segalla [UNESP]Souza, Patricia Fernanda [UNESP]Siqueira Silva, Dulce Helena [UNESP]Mendes-Giannini, Maria José Soares [UNESP]Fusco-Almeida, Ana Marisa [UNESP]2014-12-03T13:11:42Z2014-12-03T13:11:42Z2014-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://dx.doi.org/10.1155/2014/957860Evidence-based Complementary And Alternative Medicine. New York: Hindawi Publishing Corporation, 9 p., 2014.1741-427Xhttp://hdl.handle.net/11449/11343110.1155/2014/957860WOS:000338078800001WOS000338078800001.pdf0000-0002-8059-0826Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEvidence-based Complementary and Alternative Medicine2.064info:eu-repo/semantics/openAccess2024-06-21T15:19:30Zoai:repositorio.unesp.br:11449/113431Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:11:57.983390Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Anti-Trichophyton Activity of Protocatechuates and Their Synergism with Fluconazole
title Anti-Trichophyton Activity of Protocatechuates and Their Synergism with Fluconazole
spellingShingle Anti-Trichophyton Activity of Protocatechuates and Their Synergism with Fluconazole
Soares, Luciana Arantes [UNESP]
title_short Anti-Trichophyton Activity of Protocatechuates and Their Synergism with Fluconazole
title_full Anti-Trichophyton Activity of Protocatechuates and Their Synergism with Fluconazole
title_fullStr Anti-Trichophyton Activity of Protocatechuates and Their Synergism with Fluconazole
title_full_unstemmed Anti-Trichophyton Activity of Protocatechuates and Their Synergism with Fluconazole
title_sort Anti-Trichophyton Activity of Protocatechuates and Their Synergism with Fluconazole
author Soares, Luciana Arantes [UNESP]
author_facet Soares, Luciana Arantes [UNESP]
Gullo, Fernanda Patricia [UNESP]
Orlandi Sardi, Janaina de Cassia [UNESP]
Pitangui, Nayla de Souza [UNESP]
Costa-Orlandi, Caroline Barcelos [UNESP]
Sangalli-Leite, Fernanda [UNESP]
Scorzoni, Liliana [UNESP]
Regasini, Luis Octavio [UNESP]
Petronio, Maicon Segalla [UNESP]
Souza, Patricia Fernanda [UNESP]
Siqueira Silva, Dulce Helena [UNESP]
Mendes-Giannini, Maria José Soares [UNESP]
Fusco-Almeida, Ana Marisa [UNESP]
author_role author
author2 Gullo, Fernanda Patricia [UNESP]
Orlandi Sardi, Janaina de Cassia [UNESP]
Pitangui, Nayla de Souza [UNESP]
Costa-Orlandi, Caroline Barcelos [UNESP]
Sangalli-Leite, Fernanda [UNESP]
Scorzoni, Liliana [UNESP]
Regasini, Luis Octavio [UNESP]
Petronio, Maicon Segalla [UNESP]
Souza, Patricia Fernanda [UNESP]
Siqueira Silva, Dulce Helena [UNESP]
Mendes-Giannini, Maria José Soares [UNESP]
Fusco-Almeida, Ana Marisa [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Soares, Luciana Arantes [UNESP]
Gullo, Fernanda Patricia [UNESP]
Orlandi Sardi, Janaina de Cassia [UNESP]
Pitangui, Nayla de Souza [UNESP]
Costa-Orlandi, Caroline Barcelos [UNESP]
Sangalli-Leite, Fernanda [UNESP]
Scorzoni, Liliana [UNESP]
Regasini, Luis Octavio [UNESP]
Petronio, Maicon Segalla [UNESP]
Souza, Patricia Fernanda [UNESP]
Siqueira Silva, Dulce Helena [UNESP]
Mendes-Giannini, Maria José Soares [UNESP]
Fusco-Almeida, Ana Marisa [UNESP]
description Dermatophytosis and superficial mycosis are a major global public health problem that affects 20-25% of the world's population. The increase in fungal resistance to the commercially available antifungal agents, in conjunction with the limited spectrum of action of such drugs, emphasises the need to develop new antifungal agents. Natural products are attractive prototypes for antifungal agents due to their broad spectrum of biological activities. This study aimed to verify the antifungal activity of protocatechuic acid, 3,4-diacetoxybenzoic, and fourteen alkyl protocatechuates (3,4-dihydroxybenzoates) against Trichophyton rubrum and Trichophyton mentagrophytes and to further assess their activities when combined with fluconazole. Susceptibility and synergism assays were conducted as described in M38-A2 (CLSI), with modifications. Three strains of Trichophyton rubrum and three strains of Trichophyton mentagrophytes were used in this work. The pentyl, hexyl, heptyl, octyl, nonyl, and decyl protocatechuates showed great fungicidal effects, with minimum inhibitory concentration (MIC) values ranging from 0.97 to 7.8 mg/L. Heptyl showed a synergistic activity (FIC index = 0.49), reducing the MIC of fluconazole by fourfold. All substances tested were safe, especially the hexyl, heptyl, octyl, and nonyl compounds, all of which showed a high selectivity index, particularly in combination with fluconazole. These ester associations with fluconazole may represent a promising source of prototypes in the search for anti-Trichophyton therapeutic agents.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-03T13:11:42Z
2014-12-03T13:11:42Z
2014-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1155/2014/957860
Evidence-based Complementary And Alternative Medicine. New York: Hindawi Publishing Corporation, 9 p., 2014.
1741-427X
http://hdl.handle.net/11449/113431
10.1155/2014/957860
WOS:000338078800001
WOS000338078800001.pdf
0000-0002-8059-0826
url http://dx.doi.org/10.1155/2014/957860
http://hdl.handle.net/11449/113431
identifier_str_mv Evidence-based Complementary And Alternative Medicine. New York: Hindawi Publishing Corporation, 9 p., 2014.
1741-427X
10.1155/2014/957860
WOS:000338078800001
WOS000338078800001.pdf
0000-0002-8059-0826
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Evidence-based Complementary and Alternative Medicine
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application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
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