β-endorphin immunoreactivity during anaesthesia in equidae
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1046/j.1467-2995.2000.00004.x http://hdl.handle.net/11449/219005 |
Resumo: | Objective To determine the effects of surgery, hypoxia, hypercapnia and flunixin administration on plasma β-endorphin immunoreactivity (BEI) in anaesthetized horses. Study design Prospective crossover study. Animals Six healthy adult Welsh Mountain ponies and seven healthy adult Thoroughbreds. Methods Ponies were anaesthetized with thiopentone and halothane or with pentobarbitone and the horses with guaiphenesin, thiopentone and halothane. Ponies were anaesthetized for 2 hours and on separate occasions underwent a period of hypoxia, hypercapnia, anaesthesia only, or were given flunixin at induction. The horses were anaesthetized for 2 hours and on separate occasions underwent surgery to relocate one carotid artery subcutaneously or anaesthesia only. Plasma samples were taken pre-anaesthesia, at 20 minute intervals during, and after anaesthesia for BEI assay using radio-immunoassay. Analysis of variance of the concentration-time curve was used for statistical analysis. Results Pre-anaesthetic β-endorphin immunoreactivity (BEI) values ranged between 5.7 and 20.4pmol L−1.Induction of anaesthesia caused a five to 10 fold increase in mean plasma BEI in all cases except the hypercapnia group. Halothane anaesthesia increased BEI in ponies and horses but there were no significant changes during pentobarbitone anaesthesia. The increase in BEI in the hypoxic group was greater (peak value 136.8± 32.2pmol L−1) and sustained for a longer period compared with levels in those given halothane alone or in those which became hypercapnic. There was marked individual variation in the flunixin group and changes were not significant. Surgery in the horses resulted in the highest peak values in the study (182.5±153.0pmol L−1) but the AUC was not significantly higher than in the same animals without surgery, where the peak value was 102.9±42.1pmol L−1.Conclusions Beta-endorphin appeared to be a sensitive marker of an endocrine stress response but its physiological role during equine anaesthesia is unknown. Clinical relevance Unknown. © 2017 Wiley. All rights reserved. |
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β-endorphin immunoreactivity during anaesthesia in equidaeAnaesthesiaHorseStress responseβ-endorphin immunoreactivityObjective To determine the effects of surgery, hypoxia, hypercapnia and flunixin administration on plasma β-endorphin immunoreactivity (BEI) in anaesthetized horses. Study design Prospective crossover study. Animals Six healthy adult Welsh Mountain ponies and seven healthy adult Thoroughbreds. Methods Ponies were anaesthetized with thiopentone and halothane or with pentobarbitone and the horses with guaiphenesin, thiopentone and halothane. Ponies were anaesthetized for 2 hours and on separate occasions underwent a period of hypoxia, hypercapnia, anaesthesia only, or were given flunixin at induction. The horses were anaesthetized for 2 hours and on separate occasions underwent surgery to relocate one carotid artery subcutaneously or anaesthesia only. Plasma samples were taken pre-anaesthesia, at 20 minute intervals during, and after anaesthesia for BEI assay using radio-immunoassay. Analysis of variance of the concentration-time curve was used for statistical analysis. Results Pre-anaesthetic β-endorphin immunoreactivity (BEI) values ranged between 5.7 and 20.4pmol L−1.Induction of anaesthesia caused a five to 10 fold increase in mean plasma BEI in all cases except the hypercapnia group. Halothane anaesthesia increased BEI in ponies and horses but there were no significant changes during pentobarbitone anaesthesia. The increase in BEI in the hypoxic group was greater (peak value 136.8± 32.2pmol L−1) and sustained for a longer period compared with levels in those given halothane alone or in those which became hypercapnic. There was marked individual variation in the flunixin group and changes were not significant. Surgery in the horses resulted in the highest peak values in the study (182.5±153.0pmol L−1) but the AUC was not significantly higher than in the same animals without surgery, where the peak value was 102.9±42.1pmol L−1.Conclusions Beta-endorphin appeared to be a sensitive marker of an endocrine stress response but its physiological role during equine anaesthesia is unknown. Clinical relevance Unknown. © 2017 Wiley. All rights reserved.Department of Clinical Veterinary Medicine University of CambridgeDip ECVA Faculty of Veterinary Medicine and Animal Science UNESP, Campus de Botucatu, Botucatu, São Paulo, CEP 18618–000A Dye AIBMS Department of Anaesthesia Bristol Royal Infirmary University of BristolDip ECVA Faculty of Veterinary Medicine and Animal Science UNESP, Campus de Botucatu, Botucatu, São Paulo, CEP 18618–000University of CambridgeUniversidade Estadual Paulista (UNESP)University of BristolTaylor, P. M. [UNESP]Vet, M. A. [UNESP]2022-04-28T18:53:19Z2022-04-28T18:53:19Z2000-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article27-35http://dx.doi.org/10.1046/j.1467-2995.2000.00004.xVeterinary Anaesthesia and Analgesia, v. 27, n. 1, p. 27-35, 2000.1467-29951467-2987http://hdl.handle.net/11449/21900510.1046/j.1467-2995.2000.00004.x2-s2.0-0001997814Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Anaesthesia and Analgesiainfo:eu-repo/semantics/openAccess2022-04-28T18:53:19Zoai:repositorio.unesp.br:11449/219005Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:33:14.944237Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
β-endorphin immunoreactivity during anaesthesia in equidae |
title |
β-endorphin immunoreactivity during anaesthesia in equidae |
spellingShingle |
β-endorphin immunoreactivity during anaesthesia in equidae Taylor, P. M. [UNESP] Anaesthesia Horse Stress response β-endorphin immunoreactivity |
title_short |
β-endorphin immunoreactivity during anaesthesia in equidae |
title_full |
β-endorphin immunoreactivity during anaesthesia in equidae |
title_fullStr |
β-endorphin immunoreactivity during anaesthesia in equidae |
title_full_unstemmed |
β-endorphin immunoreactivity during anaesthesia in equidae |
title_sort |
β-endorphin immunoreactivity during anaesthesia in equidae |
author |
Taylor, P. M. [UNESP] |
author_facet |
Taylor, P. M. [UNESP] Vet, M. A. [UNESP] |
author_role |
author |
author2 |
Vet, M. A. [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
University of Cambridge Universidade Estadual Paulista (UNESP) University of Bristol |
dc.contributor.author.fl_str_mv |
Taylor, P. M. [UNESP] Vet, M. A. [UNESP] |
dc.subject.por.fl_str_mv |
Anaesthesia Horse Stress response β-endorphin immunoreactivity |
topic |
Anaesthesia Horse Stress response β-endorphin immunoreactivity |
description |
Objective To determine the effects of surgery, hypoxia, hypercapnia and flunixin administration on plasma β-endorphin immunoreactivity (BEI) in anaesthetized horses. Study design Prospective crossover study. Animals Six healthy adult Welsh Mountain ponies and seven healthy adult Thoroughbreds. Methods Ponies were anaesthetized with thiopentone and halothane or with pentobarbitone and the horses with guaiphenesin, thiopentone and halothane. Ponies were anaesthetized for 2 hours and on separate occasions underwent a period of hypoxia, hypercapnia, anaesthesia only, or were given flunixin at induction. The horses were anaesthetized for 2 hours and on separate occasions underwent surgery to relocate one carotid artery subcutaneously or anaesthesia only. Plasma samples were taken pre-anaesthesia, at 20 minute intervals during, and after anaesthesia for BEI assay using radio-immunoassay. Analysis of variance of the concentration-time curve was used for statistical analysis. Results Pre-anaesthetic β-endorphin immunoreactivity (BEI) values ranged between 5.7 and 20.4pmol L−1.Induction of anaesthesia caused a five to 10 fold increase in mean plasma BEI in all cases except the hypercapnia group. Halothane anaesthesia increased BEI in ponies and horses but there were no significant changes during pentobarbitone anaesthesia. The increase in BEI in the hypoxic group was greater (peak value 136.8± 32.2pmol L−1) and sustained for a longer period compared with levels in those given halothane alone or in those which became hypercapnic. There was marked individual variation in the flunixin group and changes were not significant. Surgery in the horses resulted in the highest peak values in the study (182.5±153.0pmol L−1) but the AUC was not significantly higher than in the same animals without surgery, where the peak value was 102.9±42.1pmol L−1.Conclusions Beta-endorphin appeared to be a sensitive marker of an endocrine stress response but its physiological role during equine anaesthesia is unknown. Clinical relevance Unknown. © 2017 Wiley. All rights reserved. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-01-01 2022-04-28T18:53:19Z 2022-04-28T18:53:19Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1046/j.1467-2995.2000.00004.x Veterinary Anaesthesia and Analgesia, v. 27, n. 1, p. 27-35, 2000. 1467-2995 1467-2987 http://hdl.handle.net/11449/219005 10.1046/j.1467-2995.2000.00004.x 2-s2.0-0001997814 |
url |
http://dx.doi.org/10.1046/j.1467-2995.2000.00004.x http://hdl.handle.net/11449/219005 |
identifier_str_mv |
Veterinary Anaesthesia and Analgesia, v. 27, n. 1, p. 27-35, 2000. 1467-2995 1467-2987 10.1046/j.1467-2995.2000.00004.x 2-s2.0-0001997814 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veterinary Anaesthesia and Analgesia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
27-35 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129334839345152 |