The mutagenic potential of Clusia alata (Clusiaceae) extract based on two short-term in vivo assays

Detalhes bibliográficos
Autor(a) principal: Moura, A. C. G. [UNESP]
Data de Publicação: 2008
Outros Autores: Perazzo, F. F. [UNESP], Maistro, Edson Luis [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.4238/vol7-4gmr523
http://hdl.handle.net/11449/10808
Resumo: We examined the genotoxic and mutagenic effects of a crude extract of Clusia alata ( a potential medicinal plant) on peripheral leukocyte and bone marrow cells of mice, using the comet and chromosome aberration assays. Extracts at doses of 1000, 1500 and 2000 mg/kg were administered by gavage, and a positive control, N-nitroso-N-ethylurea (50 mg/kg) was injected intraperitoneally. Peripheral blood leukocytes were collected 4 and 24 h after the treatments for the comet assay, and bone marrow cells were collected 24 h after the treatments, for the chromosome aberration assay. The comet assay showed that C. alata extract causes an increase in damage to DNA in the peripheral blood leukocytes, but it was significant only with the 2000 mg/kg dose after 24 h; the extract also induced a small but significant increase in the mean number of chromosome aberrations in the bone marrow cells at doses of 1500 and 2000 mg/kg. No evidence of a significant decrease in the mitotic index was observed. Acute consumption of high concentrations of C. alata extract produced some mutagenic effects in bone marrow cells.
id UNSP_e63f8e7d3de7f9e0352ccbe82bb7007f
oai_identifier_str oai:repositorio.unesp.br:11449/10808
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling The mutagenic potential of Clusia alata (Clusiaceae) extract based on two short-term in vivo assaysClusia alataComet assayChromosome aberrationsSingle-cell gel electrophoresisWe examined the genotoxic and mutagenic effects of a crude extract of Clusia alata ( a potential medicinal plant) on peripheral leukocyte and bone marrow cells of mice, using the comet and chromosome aberration assays. Extracts at doses of 1000, 1500 and 2000 mg/kg were administered by gavage, and a positive control, N-nitroso-N-ethylurea (50 mg/kg) was injected intraperitoneally. Peripheral blood leukocytes were collected 4 and 24 h after the treatments for the comet assay, and bone marrow cells were collected 24 h after the treatments, for the chromosome aberration assay. The comet assay showed that C. alata extract causes an increase in damage to DNA in the peripheral blood leukocytes, but it was significant only with the 2000 mg/kg dose after 24 h; the extract also induced a small but significant increase in the mean number of chromosome aberrations in the bone marrow cells at doses of 1500 and 2000 mg/kg. No evidence of a significant decrease in the mitotic index was observed. Acute consumption of high concentrations of C. alata extract produced some mutagenic effects in bone marrow cells.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Fac Filosofia & Ciencias, Dept Fonoaudiol, Marilia, SP, BrazilUniv Fed Amapa, Lab Pesquisa Farmacos, Macapa, AP, BrazilUniv Estadual Paulista, Fac Filosofia & Ciencias, Dept Fonoaudiol, Marilia, SP, BrazilCNPq: 306544/2006-7FAPESP: 06/57514-2Funpec-editoraUniversidade Estadual Paulista (Unesp)Universidade Federal do Amapá (UNIFAP)Moura, A. C. G. [UNESP]Perazzo, F. F. [UNESP]Maistro, Edson Luis [UNESP]2014-05-20T13:31:44Z2014-05-20T13:31:44Z2008-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1360-1368application/pdfhttp://dx.doi.org/10.4238/vol7-4gmr523Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 7, n. 4, p. 1360-1368, 2008.1676-5680http://hdl.handle.net/11449/1080810.4238/vol7-4gmr523WOS:000267218200025WOS000267218200025.pdf47875216130383150000-0003-0757-7876Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Research0,439info:eu-repo/semantics/openAccess2024-08-09T17:39:30Zoai:repositorio.unesp.br:11449/10808Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-09T17:39:30Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv The mutagenic potential of Clusia alata (Clusiaceae) extract based on two short-term in vivo assays
title The mutagenic potential of Clusia alata (Clusiaceae) extract based on two short-term in vivo assays
spellingShingle The mutagenic potential of Clusia alata (Clusiaceae) extract based on two short-term in vivo assays
Moura, A. C. G. [UNESP]
Clusia alata
Comet assay
Chromosome aberrations
Single-cell gel electrophoresis
title_short The mutagenic potential of Clusia alata (Clusiaceae) extract based on two short-term in vivo assays
title_full The mutagenic potential of Clusia alata (Clusiaceae) extract based on two short-term in vivo assays
title_fullStr The mutagenic potential of Clusia alata (Clusiaceae) extract based on two short-term in vivo assays
title_full_unstemmed The mutagenic potential of Clusia alata (Clusiaceae) extract based on two short-term in vivo assays
title_sort The mutagenic potential of Clusia alata (Clusiaceae) extract based on two short-term in vivo assays
author Moura, A. C. G. [UNESP]
author_facet Moura, A. C. G. [UNESP]
Perazzo, F. F. [UNESP]
Maistro, Edson Luis [UNESP]
author_role author
author2 Perazzo, F. F. [UNESP]
Maistro, Edson Luis [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal do Amapá (UNIFAP)
dc.contributor.author.fl_str_mv Moura, A. C. G. [UNESP]
Perazzo, F. F. [UNESP]
Maistro, Edson Luis [UNESP]
dc.subject.por.fl_str_mv Clusia alata
Comet assay
Chromosome aberrations
Single-cell gel electrophoresis
topic Clusia alata
Comet assay
Chromosome aberrations
Single-cell gel electrophoresis
description We examined the genotoxic and mutagenic effects of a crude extract of Clusia alata ( a potential medicinal plant) on peripheral leukocyte and bone marrow cells of mice, using the comet and chromosome aberration assays. Extracts at doses of 1000, 1500 and 2000 mg/kg were administered by gavage, and a positive control, N-nitroso-N-ethylurea (50 mg/kg) was injected intraperitoneally. Peripheral blood leukocytes were collected 4 and 24 h after the treatments for the comet assay, and bone marrow cells were collected 24 h after the treatments, for the chromosome aberration assay. The comet assay showed that C. alata extract causes an increase in damage to DNA in the peripheral blood leukocytes, but it was significant only with the 2000 mg/kg dose after 24 h; the extract also induced a small but significant increase in the mean number of chromosome aberrations in the bone marrow cells at doses of 1500 and 2000 mg/kg. No evidence of a significant decrease in the mitotic index was observed. Acute consumption of high concentrations of C. alata extract produced some mutagenic effects in bone marrow cells.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
2014-05-20T13:31:44Z
2014-05-20T13:31:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4238/vol7-4gmr523
Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 7, n. 4, p. 1360-1368, 2008.
1676-5680
http://hdl.handle.net/11449/10808
10.4238/vol7-4gmr523
WOS:000267218200025
WOS000267218200025.pdf
4787521613038315
0000-0003-0757-7876
url http://dx.doi.org/10.4238/vol7-4gmr523
http://hdl.handle.net/11449/10808
identifier_str_mv Genetics and Molecular Research. Ribeirao Preto: Funpec-editora, v. 7, n. 4, p. 1360-1368, 2008.
1676-5680
10.4238/vol7-4gmr523
WOS:000267218200025
WOS000267218200025.pdf
4787521613038315
0000-0003-0757-7876
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Genetics and Molecular Research
0,439
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1360-1368
application/pdf
dc.publisher.none.fl_str_mv Funpec-editora
publisher.none.fl_str_mv Funpec-editora
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128168764112896