Investigation of canine visceral leishmaniasis in a non-endemic area in Brazil and the comparison of serological and molecular diagnostic tests
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/0037-8682-0182-2021 http://hdl.handle.net/11449/233600 |
Resumo: | INTRODUCTION: Visceral leishmaniasis (VL) is an important zoonosis in Brazil. Previous identification of parasitized dogs can also help prevent the disease in humans, even in non-endemic areas of the country. The Brazilian Ministry of Health recommends diagnosis in dogs using a DPP® (rapid test) as a screening test and an immunoenzymatic assay (ELISA) as a confirmatory test (DPP®+ELISA), and culling infected dogs as a legal control measure. However, the accuracy of these serological tests has been questioned. METHODS: VL in dogs was investigated in a non-endemic area of the São Paulo state for three consecutive years, and the performances of different diagnostic tests were compared. RESULTS: A total of 331 dog samples were collected in 2015, 373 in 2016, and 347 in 2017. The seroprevalence by DPP®+ELISA was 3.3, 3.2, and 0.3%, respectively, and by indirect immunofluorescence assay (IFA), it was 3.0, 5.6, and 5.5%, respectively. ELISA confirmed 18.4% of DPP® positive samples. The concordance between the IFA and DPP® was 83.9%. The concordance between IFA and DPP®+ELISA was 92.9%. A molecular diagnostic test (PCR) was performed in 63.2% of the seropositive samples, all of which were negative. CONCLUSIONS: In non-endemic areas, diagnostic tests in dogs should be carefully evaluated to avoid false results. |
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Investigation of canine visceral leishmaniasis in a non-endemic area in Brazil and the comparison of serological and molecular diagnostic testsINTRODUCTION: Visceral leishmaniasis (VL) is an important zoonosis in Brazil. Previous identification of parasitized dogs can also help prevent the disease in humans, even in non-endemic areas of the country. The Brazilian Ministry of Health recommends diagnosis in dogs using a DPP® (rapid test) as a screening test and an immunoenzymatic assay (ELISA) as a confirmatory test (DPP®+ELISA), and culling infected dogs as a legal control measure. However, the accuracy of these serological tests has been questioned. METHODS: VL in dogs was investigated in a non-endemic area of the São Paulo state for three consecutive years, and the performances of different diagnostic tests were compared. RESULTS: A total of 331 dog samples were collected in 2015, 373 in 2016, and 347 in 2017. The seroprevalence by DPP®+ELISA was 3.3, 3.2, and 0.3%, respectively, and by indirect immunofluorescence assay (IFA), it was 3.0, 5.6, and 5.5%, respectively. ELISA confirmed 18.4% of DPP® positive samples. The concordance between the IFA and DPP® was 83.9%. The concordance between IFA and DPP®+ELISA was 92.9%. A molecular diagnostic test (PCR) was performed in 63.2% of the seropositive samples, all of which were negative. CONCLUSIONS: In non-endemic areas, diagnostic tests in dogs should be carefully evaluated to avoid false results.Universidade de São Paulo Departamento de Veterinária Preventiva e Saúde AnimalUniversidade Estadual de Santa Cruz, Departamento de Ciências Agrárias e Ambientais, Ilhéus, BA, BrasilInstituto Chico Mendez de Conservação da BiodiversidadeUniversidade Estadual Paulista Júlio de Mesquita Filho Departamento de Higiene Veterinária e Saúde PúblicaInstituto Adolfo LutzUniversidade Estadual Paulista Júlio de Mesquita Filho Departamento de Higiene Veterinária e Saúde PúblicaUniversidade de São Paulo (USP)Instituto Chico Mendez de Conservação da BiodiversidadeUniversidade Estadual Paulista (UNESP)Instituto Adolfo LutzSevá, Anaiá da PaixãoBrandão, Ana Pérola DrullaGodoy, Silvia NeriSoares, Rodrigo MartinsLangoni, Helio [UNESP]Rodrigues, Bruna CristineGava, Mariana Zanchetta E [UNESP]Zanotto, Paula Ferraz de Camargo [UNESP]Jimenez-Villegas, TatianaHiramoto, RobertoFerreira, Fernando2022-05-01T09:30:55Z2022-05-01T09:30:55Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlee01822021http://dx.doi.org/10.1590/0037-8682-0182-2021Revista da Sociedade Brasileira de Medicina Tropical, v. 54, p. e01822021-.1678-9849http://hdl.handle.net/11449/23360010.1590/0037-8682-0182-20212-s2.0-85115931966Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista da Sociedade Brasileira de Medicina Tropicalinfo:eu-repo/semantics/openAccess2022-05-01T09:30:55Zoai:repositorio.unesp.br:11449/233600Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:40:17.362875Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Investigation of canine visceral leishmaniasis in a non-endemic area in Brazil and the comparison of serological and molecular diagnostic tests |
title |
Investigation of canine visceral leishmaniasis in a non-endemic area in Brazil and the comparison of serological and molecular diagnostic tests |
spellingShingle |
Investigation of canine visceral leishmaniasis in a non-endemic area in Brazil and the comparison of serological and molecular diagnostic tests Sevá, Anaiá da Paixão |
title_short |
Investigation of canine visceral leishmaniasis in a non-endemic area in Brazil and the comparison of serological and molecular diagnostic tests |
title_full |
Investigation of canine visceral leishmaniasis in a non-endemic area in Brazil and the comparison of serological and molecular diagnostic tests |
title_fullStr |
Investigation of canine visceral leishmaniasis in a non-endemic area in Brazil and the comparison of serological and molecular diagnostic tests |
title_full_unstemmed |
Investigation of canine visceral leishmaniasis in a non-endemic area in Brazil and the comparison of serological and molecular diagnostic tests |
title_sort |
Investigation of canine visceral leishmaniasis in a non-endemic area in Brazil and the comparison of serological and molecular diagnostic tests |
author |
Sevá, Anaiá da Paixão |
author_facet |
Sevá, Anaiá da Paixão Brandão, Ana Pérola Drulla Godoy, Silvia Neri Soares, Rodrigo Martins Langoni, Helio [UNESP] Rodrigues, Bruna Cristine Gava, Mariana Zanchetta E [UNESP] Zanotto, Paula Ferraz de Camargo [UNESP] Jimenez-Villegas, Tatiana Hiramoto, Roberto Ferreira, Fernando |
author_role |
author |
author2 |
Brandão, Ana Pérola Drulla Godoy, Silvia Neri Soares, Rodrigo Martins Langoni, Helio [UNESP] Rodrigues, Bruna Cristine Gava, Mariana Zanchetta E [UNESP] Zanotto, Paula Ferraz de Camargo [UNESP] Jimenez-Villegas, Tatiana Hiramoto, Roberto Ferreira, Fernando |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Instituto Chico Mendez de Conservação da Biodiversidade Universidade Estadual Paulista (UNESP) Instituto Adolfo Lutz |
dc.contributor.author.fl_str_mv |
Sevá, Anaiá da Paixão Brandão, Ana Pérola Drulla Godoy, Silvia Neri Soares, Rodrigo Martins Langoni, Helio [UNESP] Rodrigues, Bruna Cristine Gava, Mariana Zanchetta E [UNESP] Zanotto, Paula Ferraz de Camargo [UNESP] Jimenez-Villegas, Tatiana Hiramoto, Roberto Ferreira, Fernando |
description |
INTRODUCTION: Visceral leishmaniasis (VL) is an important zoonosis in Brazil. Previous identification of parasitized dogs can also help prevent the disease in humans, even in non-endemic areas of the country. The Brazilian Ministry of Health recommends diagnosis in dogs using a DPP® (rapid test) as a screening test and an immunoenzymatic assay (ELISA) as a confirmatory test (DPP®+ELISA), and culling infected dogs as a legal control measure. However, the accuracy of these serological tests has been questioned. METHODS: VL in dogs was investigated in a non-endemic area of the São Paulo state for three consecutive years, and the performances of different diagnostic tests were compared. RESULTS: A total of 331 dog samples were collected in 2015, 373 in 2016, and 347 in 2017. The seroprevalence by DPP®+ELISA was 3.3, 3.2, and 0.3%, respectively, and by indirect immunofluorescence assay (IFA), it was 3.0, 5.6, and 5.5%, respectively. ELISA confirmed 18.4% of DPP® positive samples. The concordance between the IFA and DPP® was 83.9%. The concordance between IFA and DPP®+ELISA was 92.9%. A molecular diagnostic test (PCR) was performed in 63.2% of the seropositive samples, all of which were negative. CONCLUSIONS: In non-endemic areas, diagnostic tests in dogs should be carefully evaluated to avoid false results. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-05-01T09:30:55Z 2022-05-01T09:30:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/0037-8682-0182-2021 Revista da Sociedade Brasileira de Medicina Tropical, v. 54, p. e01822021-. 1678-9849 http://hdl.handle.net/11449/233600 10.1590/0037-8682-0182-2021 2-s2.0-85115931966 |
url |
http://dx.doi.org/10.1590/0037-8682-0182-2021 http://hdl.handle.net/11449/233600 |
identifier_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical, v. 54, p. e01822021-. 1678-9849 10.1590/0037-8682-0182-2021 2-s2.0-85115931966 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista da Sociedade Brasileira de Medicina Tropical |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
e01822021 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808129449323921408 |