Physiological impacts and bioaccumulation of dietary Cu and Cd in a model teleost: The Amazonian tambaqui (Colossoma macropomum)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.aquatox.2018.03.021 http://hdl.handle.net/11449/170831 |
Resumo: | Increasing anthropogenic activities in the Amazon have led to elevated metals in the aquatic environment. Since fish are the main source of animal protein for the Amazonian population, understanding metal bioaccumulation patterns and physiological impacts is of critical importance. Juvenile tambaqui, a local model species, were exposed to chronic dietary Cu (essential, 500 μg Cu/g food) and Cd (non-essential, 500 μg Cd/g food). Fish were sampled at 10–14, 18–20 and 33–36 days of exposure and the following parameters were analyzed: growth, voluntary food consumption, conversion efficiency, tissue-specific metal bioaccumulation, ammonia and urea-N excretion, O2 consumption, Pcrit, hypoxia tolerance, nitrogen quotient, major blood plasma ions and metabolites, gill and gut enzyme activities, and in vitro gut fluid transport. The results indicate no ionoregulatory impacts of either of the metal-contaminated diets at gill, gut, or plasma levels, and no differences in plasma cortisol or lactate. The Cd diet appeared to have suppressed feeding, though overall tank growth was not affected. Bioaccumulation of both metals was observed. Distinct tissue-specific and time-specific patterns were seen. Metal burdens in the edible white muscle remained low. Overall, physiological impacts of the Cu diet were minimal. However dietary Cd increased hypoxia tolerance, as evidenced by decreased Pcrit, increased time to loss of equilibrium, a lack of plasma glucose elevation, decreased plasma ethanol, and decreased NQ during hypoxia. Blood O2 transport characteristics (P50, Bohr coefficient, hemoglobin, hematocrit) were unaffected, suggesting that tissue level changes in metabolism accounted for the greater hypoxia tolerance in tambaqui fed with a Cd-contaminated diet. |
id |
UNSP_e7c5b2d88c8274e591aa6a4af4c3b397 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/170831 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Physiological impacts and bioaccumulation of dietary Cu and Cd in a model teleost: The Amazonian tambaqui (Colossoma macropomum)Dietary metalsHypoxiaPcritTissue accumulationTropical teleostIncreasing anthropogenic activities in the Amazon have led to elevated metals in the aquatic environment. Since fish are the main source of animal protein for the Amazonian population, understanding metal bioaccumulation patterns and physiological impacts is of critical importance. Juvenile tambaqui, a local model species, were exposed to chronic dietary Cu (essential, 500 μg Cu/g food) and Cd (non-essential, 500 μg Cd/g food). Fish were sampled at 10–14, 18–20 and 33–36 days of exposure and the following parameters were analyzed: growth, voluntary food consumption, conversion efficiency, tissue-specific metal bioaccumulation, ammonia and urea-N excretion, O2 consumption, Pcrit, hypoxia tolerance, nitrogen quotient, major blood plasma ions and metabolites, gill and gut enzyme activities, and in vitro gut fluid transport. The results indicate no ionoregulatory impacts of either of the metal-contaminated diets at gill, gut, or plasma levels, and no differences in plasma cortisol or lactate. The Cd diet appeared to have suppressed feeding, though overall tank growth was not affected. Bioaccumulation of both metals was observed. Distinct tissue-specific and time-specific patterns were seen. Metal burdens in the edible white muscle remained low. Overall, physiological impacts of the Cu diet were minimal. However dietary Cd increased hypoxia tolerance, as evidenced by decreased Pcrit, increased time to loss of equilibrium, a lack of plasma glucose elevation, decreased plasma ethanol, and decreased NQ during hypoxia. Blood O2 transport characteristics (P50, Bohr coefficient, hemoglobin, hematocrit) were unaffected, suggesting that tissue level changes in metabolism accounted for the greater hypoxia tolerance in tambaqui fed with a Cd-contaminated diet.Instituto Nacional de Ciência e Tecnologia Centro de Estudos das Adaptações da Biota Aquática da AmazôniaNatural Sciences and Engineering Research Council of CanadaDepartment of Zoology The University of British ColumbiaLaboratory of Ecophysiology and Molecular Evolution Instituto Nacional de Pesquisas da Amazônia (INPA)São Paulo State University (UNESP) Institute of BiosciencesDepartment of Biology McMaster UniversityRosenstiel School of Marine and Atmospheric Science University of MiamiSão Paulo State University (UNESP) Institute of BiosciencesThe University of British ColumbiaInstituto Nacional de Pesquisas da Amazônia (INPA)Universidade Estadual Paulista (Unesp)McMaster UniversityUniversity of MiamiGiacomin, MarinaVilarinho, Gisele C.Castro, Katia F.Ferreira, MárcioDuarte, Rafael M. [UNESP]Wood, Chris M.Val, Adalberto L.2018-12-11T16:52:36Z2018-12-11T16:52:36Z2018-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article30-45application/pdfhttp://dx.doi.org/10.1016/j.aquatox.2018.03.021Aquatic Toxicology, v. 199, p. 30-45.1879-15140166-445Xhttp://hdl.handle.net/11449/17083110.1016/j.aquatox.2018.03.0212-s2.0-850445026422-s2.0-85044502642.pdf30557957777876120000-0001-5649-0692Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquatic Toxicology1,456info:eu-repo/semantics/openAccess2024-01-13T06:31:40Zoai:repositorio.unesp.br:11449/170831Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-13T06:31:40Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Physiological impacts and bioaccumulation of dietary Cu and Cd in a model teleost: The Amazonian tambaqui (Colossoma macropomum) |
title |
Physiological impacts and bioaccumulation of dietary Cu and Cd in a model teleost: The Amazonian tambaqui (Colossoma macropomum) |
spellingShingle |
Physiological impacts and bioaccumulation of dietary Cu and Cd in a model teleost: The Amazonian tambaqui (Colossoma macropomum) Giacomin, Marina Dietary metals Hypoxia Pcrit Tissue accumulation Tropical teleost |
title_short |
Physiological impacts and bioaccumulation of dietary Cu and Cd in a model teleost: The Amazonian tambaqui (Colossoma macropomum) |
title_full |
Physiological impacts and bioaccumulation of dietary Cu and Cd in a model teleost: The Amazonian tambaqui (Colossoma macropomum) |
title_fullStr |
Physiological impacts and bioaccumulation of dietary Cu and Cd in a model teleost: The Amazonian tambaqui (Colossoma macropomum) |
title_full_unstemmed |
Physiological impacts and bioaccumulation of dietary Cu and Cd in a model teleost: The Amazonian tambaqui (Colossoma macropomum) |
title_sort |
Physiological impacts and bioaccumulation of dietary Cu and Cd in a model teleost: The Amazonian tambaqui (Colossoma macropomum) |
author |
Giacomin, Marina |
author_facet |
Giacomin, Marina Vilarinho, Gisele C. Castro, Katia F. Ferreira, Márcio Duarte, Rafael M. [UNESP] Wood, Chris M. Val, Adalberto L. |
author_role |
author |
author2 |
Vilarinho, Gisele C. Castro, Katia F. Ferreira, Márcio Duarte, Rafael M. [UNESP] Wood, Chris M. Val, Adalberto L. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
The University of British Columbia Instituto Nacional de Pesquisas da Amazônia (INPA) Universidade Estadual Paulista (Unesp) McMaster University University of Miami |
dc.contributor.author.fl_str_mv |
Giacomin, Marina Vilarinho, Gisele C. Castro, Katia F. Ferreira, Márcio Duarte, Rafael M. [UNESP] Wood, Chris M. Val, Adalberto L. |
dc.subject.por.fl_str_mv |
Dietary metals Hypoxia Pcrit Tissue accumulation Tropical teleost |
topic |
Dietary metals Hypoxia Pcrit Tissue accumulation Tropical teleost |
description |
Increasing anthropogenic activities in the Amazon have led to elevated metals in the aquatic environment. Since fish are the main source of animal protein for the Amazonian population, understanding metal bioaccumulation patterns and physiological impacts is of critical importance. Juvenile tambaqui, a local model species, were exposed to chronic dietary Cu (essential, 500 μg Cu/g food) and Cd (non-essential, 500 μg Cd/g food). Fish were sampled at 10–14, 18–20 and 33–36 days of exposure and the following parameters were analyzed: growth, voluntary food consumption, conversion efficiency, tissue-specific metal bioaccumulation, ammonia and urea-N excretion, O2 consumption, Pcrit, hypoxia tolerance, nitrogen quotient, major blood plasma ions and metabolites, gill and gut enzyme activities, and in vitro gut fluid transport. The results indicate no ionoregulatory impacts of either of the metal-contaminated diets at gill, gut, or plasma levels, and no differences in plasma cortisol or lactate. The Cd diet appeared to have suppressed feeding, though overall tank growth was not affected. Bioaccumulation of both metals was observed. Distinct tissue-specific and time-specific patterns were seen. Metal burdens in the edible white muscle remained low. Overall, physiological impacts of the Cu diet were minimal. However dietary Cd increased hypoxia tolerance, as evidenced by decreased Pcrit, increased time to loss of equilibrium, a lack of plasma glucose elevation, decreased plasma ethanol, and decreased NQ during hypoxia. Blood O2 transport characteristics (P50, Bohr coefficient, hemoglobin, hematocrit) were unaffected, suggesting that tissue level changes in metabolism accounted for the greater hypoxia tolerance in tambaqui fed with a Cd-contaminated diet. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T16:52:36Z 2018-12-11T16:52:36Z 2018-06-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.aquatox.2018.03.021 Aquatic Toxicology, v. 199, p. 30-45. 1879-1514 0166-445X http://hdl.handle.net/11449/170831 10.1016/j.aquatox.2018.03.021 2-s2.0-85044502642 2-s2.0-85044502642.pdf 3055795777787612 0000-0001-5649-0692 |
url |
http://dx.doi.org/10.1016/j.aquatox.2018.03.021 http://hdl.handle.net/11449/170831 |
identifier_str_mv |
Aquatic Toxicology, v. 199, p. 30-45. 1879-1514 0166-445X 10.1016/j.aquatox.2018.03.021 2-s2.0-85044502642 2-s2.0-85044502642.pdf 3055795777787612 0000-0001-5649-0692 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Aquatic Toxicology 1,456 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
30-45 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803650244225794048 |