Candida spp.: manual identification (reference method) and automated identification (Vitek system platform)
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1678-91992012000300011 http://hdl.handle.net/11449/11365 |
Resumo: | Yeasts are becoming a common cause of nosocomial fungal infections that affect immunocompromised patients. Such infections can evolve into sepsis, whose mortality rate is high. This study aimed to evaluate the viability of Candida species identification by the automated system Vitek-Biomerieux (Durham, USA). Ninety-eight medical charts referencing the Candida spp. samples available for the study were retrospectively analyzed. The system Vitek-Biomerieux with Candida identification card is recommended for laboratory routine use and presents 80.6% agreement with the reference method. By separate analysis of species, 13.5% of C. parapsilosis samples differed from the reference method, while the Vitek system wrongly identified them as C. tropicalis, C. lusitaneae or as Candida albicans. C. glabrata presented a discrepancy of only one sample (25%), and was identified by Vitek as C. parapsilosis. C. guilliermondii also differed in only one sample (33.3%), being identified as Candida spp. All C. albicans, C. tropicalis and C. lusitaneae samples were identified correctly. |
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Candida spp.: manual identification (reference method) and automated identification (Vitek system platform)candidemiaCandida spp.identificationautomated identificationYeasts are becoming a common cause of nosocomial fungal infections that affect immunocompromised patients. Such infections can evolve into sepsis, whose mortality rate is high. This study aimed to evaluate the viability of Candida species identification by the automated system Vitek-Biomerieux (Durham, USA). Ninety-eight medical charts referencing the Candida spp. samples available for the study were retrospectively analyzed. The system Vitek-Biomerieux with Candida identification card is recommended for laboratory routine use and presents 80.6% agreement with the reference method. By separate analysis of species, 13.5% of C. parapsilosis samples differed from the reference method, while the Vitek system wrongly identified them as C. tropicalis, C. lusitaneae or as Candida albicans. C. glabrata presented a discrepancy of only one sample (25%), and was identified by Vitek as C. parapsilosis. C. guilliermondii also differed in only one sample (33.3%), being identified as Candida spp. All C. albicans, C. tropicalis and C. lusitaneae samples were identified correctly.Fundação para o Desenvolvimento Médico e Hospitalar (Famesp)Clinical Hospital and Clinical Analysis Laboratory of Botucatu Medical SchoolDepartment of Microbiology and Immunology of the Botucatu Biosciences Institute, UNESP, Botucatu, SPUniv Estadual Paulista, São Paulo State Univ, Dept Med Interna, Fac Med Botucatu,Botucatu Med Sch,UNESP, BR-18618970 Botucatu, SP, BrazilUniv Estadual Paulista, São Paulo State Univ, Dept Microbiol & Immunol, Botucatu Biosci Inst,UNESP, BR-18618970 Botucatu, SP, BrazilUniv Estadual Paulista, São Paulo State Univ, Dept Med Interna, Fac Med Botucatu,Botucatu Med Sch,UNESP, BR-18618970 Botucatu, SP, BrazilUniv Estadual Paulista, São Paulo State Univ, Dept Microbiol & Immunol, Botucatu Biosci Inst,UNESP, BR-18618970 Botucatu, SP, BrazilUniversidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP)Universidade Estadual Paulista (Unesp)Mondelli, A. L. [UNESP]Niero-Melo, L.Bagagli, Eduardo [UNESP]Camargo, C. H. [UNESP]Bruder-Nascimento, A. [UNESP]Sugizaki, M. F. [UNESP]Carneiro, M.Villas Boas, P. J. F.2014-05-20T13:33:15Z2014-05-20T13:33:15Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article335-339application/pdfhttp://dx.doi.org/10.1590/S1678-91992012000300011Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 18, n. 3, p. 335-339, 2012.1678-9199http://hdl.handle.net/11449/11365S1678-91992012000300011WOS:000308950400011S1678-91992012000300011.pdf33203275704295390000-0002-8003-4109Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Venomous Animals and Toxins Including Tropical Diseases1.7820,573info:eu-repo/semantics/openAccess2023-12-21T06:21:24Zoai:repositorio.unesp.br:11449/11365Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:54:57.024868Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Candida spp.: manual identification (reference method) and automated identification (Vitek system platform) |
title |
Candida spp.: manual identification (reference method) and automated identification (Vitek system platform) |
spellingShingle |
Candida spp.: manual identification (reference method) and automated identification (Vitek system platform) Mondelli, A. L. [UNESP] candidemia Candida spp. identification automated identification |
title_short |
Candida spp.: manual identification (reference method) and automated identification (Vitek system platform) |
title_full |
Candida spp.: manual identification (reference method) and automated identification (Vitek system platform) |
title_fullStr |
Candida spp.: manual identification (reference method) and automated identification (Vitek system platform) |
title_full_unstemmed |
Candida spp.: manual identification (reference method) and automated identification (Vitek system platform) |
title_sort |
Candida spp.: manual identification (reference method) and automated identification (Vitek system platform) |
author |
Mondelli, A. L. [UNESP] |
author_facet |
Mondelli, A. L. [UNESP] Niero-Melo, L. Bagagli, Eduardo [UNESP] Camargo, C. H. [UNESP] Bruder-Nascimento, A. [UNESP] Sugizaki, M. F. [UNESP] Carneiro, M. Villas Boas, P. J. F. |
author_role |
author |
author2 |
Niero-Melo, L. Bagagli, Eduardo [UNESP] Camargo, C. H. [UNESP] Bruder-Nascimento, A. [UNESP] Sugizaki, M. F. [UNESP] Carneiro, M. Villas Boas, P. J. F. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Mondelli, A. L. [UNESP] Niero-Melo, L. Bagagli, Eduardo [UNESP] Camargo, C. H. [UNESP] Bruder-Nascimento, A. [UNESP] Sugizaki, M. F. [UNESP] Carneiro, M. Villas Boas, P. J. F. |
dc.subject.por.fl_str_mv |
candidemia Candida spp. identification automated identification |
topic |
candidemia Candida spp. identification automated identification |
description |
Yeasts are becoming a common cause of nosocomial fungal infections that affect immunocompromised patients. Such infections can evolve into sepsis, whose mortality rate is high. This study aimed to evaluate the viability of Candida species identification by the automated system Vitek-Biomerieux (Durham, USA). Ninety-eight medical charts referencing the Candida spp. samples available for the study were retrospectively analyzed. The system Vitek-Biomerieux with Candida identification card is recommended for laboratory routine use and presents 80.6% agreement with the reference method. By separate analysis of species, 13.5% of C. parapsilosis samples differed from the reference method, while the Vitek system wrongly identified them as C. tropicalis, C. lusitaneae or as Candida albicans. C. glabrata presented a discrepancy of only one sample (25%), and was identified by Vitek as C. parapsilosis. C. guilliermondii also differed in only one sample (33.3%), being identified as Candida spp. All C. albicans, C. tropicalis and C. lusitaneae samples were identified correctly. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 2014-05-20T13:33:15Z 2014-05-20T13:33:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1678-91992012000300011 Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 18, n. 3, p. 335-339, 2012. 1678-9199 http://hdl.handle.net/11449/11365 S1678-91992012000300011 WOS:000308950400011 S1678-91992012000300011.pdf 3320327570429539 0000-0002-8003-4109 |
url |
http://dx.doi.org/10.1590/S1678-91992012000300011 http://hdl.handle.net/11449/11365 |
identifier_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases. Botucatu: Cevap-unesp, v. 18, n. 3, p. 335-339, 2012. 1678-9199 S1678-91992012000300011 WOS:000308950400011 S1678-91992012000300011.pdf 3320327570429539 0000-0002-8003-4109 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Venomous Animals and Toxins Including Tropical Diseases 1.782 0,573 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
335-339 application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp), Centro de Estudos de Venenos e Animais Peçonhentos (CEVAP) |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129262613430272 |