Genetic differentiation between sympatric populations of Bacillus cereus and Bacillus thuringiensis
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1128/AEM.68.3.1414-1424.2002 http://hdl.handle.net/11449/931 |
Resumo: | Little is known about genetic exchanges in natural populations of bacteria of the spore-forming Bacillus cereus group, because no population genetics studies have been performed with local sympatric populations. We isolated strains of Bacillus thuringiensis and B. cereus from small samples of soil collected at the same time from two separate geographical sites, one within the forest and the other at the edge of the forest. A total of 100 B. cercus and 98 B. thuringiensis strains were isolated and characterized by electrophoresis to determine allelic composition at nine enzymatic loci. We observed genetic differentiation between populations of B. cereus and B. thuringiensis. Populations of a given Bacillus species-B. thuringiensis or B. cereus-were genetically more similar to each other than to populations of the other Bacillus species. Hemolytic activity provided further evidence of this genetic divergence, which remained evident even if putative clones were removed from the data set. Our results suggest that the rate of gene flow was higher between strains of the same species, but that exchanges between B. cereus and B. thuringiensis were nonetheless possible. Linkage disequilibrium analysis revealed sufficient recombination for B. cereus populations to be considered panmictic units. In B. thuringiensis, the balance between clonal proliferation and recombination seemed to depend on location. Overall, our data indicate that it is not important for risk assessment purposes to determine whether B. cereus and B. thuringiensis belong to a single or two species. Assessment of the biosafety of pest control based on B. thuringiensis requires evaluation of the extent of genetic exchange between strains in realistic natural conditions. |
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Repositório Institucional da UNESP |
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spelling |
Genetic differentiation between sympatric populations of Bacillus cereus and Bacillus thuringiensisLittle is known about genetic exchanges in natural populations of bacteria of the spore-forming Bacillus cereus group, because no population genetics studies have been performed with local sympatric populations. We isolated strains of Bacillus thuringiensis and B. cereus from small samples of soil collected at the same time from two separate geographical sites, one within the forest and the other at the edge of the forest. A total of 100 B. cercus and 98 B. thuringiensis strains were isolated and characterized by electrophoresis to determine allelic composition at nine enzymatic loci. We observed genetic differentiation between populations of B. cereus and B. thuringiensis. Populations of a given Bacillus species-B. thuringiensis or B. cereus-were genetically more similar to each other than to populations of the other Bacillus species. Hemolytic activity provided further evidence of this genetic divergence, which remained evident even if putative clones were removed from the data set. Our results suggest that the rate of gene flow was higher between strains of the same species, but that exchanges between B. cereus and B. thuringiensis were nonetheless possible. Linkage disequilibrium analysis revealed sufficient recombination for B. cereus populations to be considered panmictic units. In B. thuringiensis, the balance between clonal proliferation and recombination seemed to depend on location. Overall, our data indicate that it is not important for risk assessment purposes to determine whether B. cereus and B. thuringiensis belong to a single or two species. Assessment of the biosafety of pest control based on B. thuringiensis requires evaluation of the extent of genetic exchange between strains in realistic natural conditions.Inst Natl Rech Agron, Unite Rech Lutte Biol, F-78285 Guyancourt, FranceInst Pasteur, CNRS URA 2172, Unite Biochim Microbienne, F-75724 Paris, FranceUNESP, FCAV, Dept Biol Aplicada Agropecuaria, BR-14884900 Jaboticabal, SP, BrazilUNESP, FCAV, Dept Biol Aplicada Agropecuaria, BR-14884900 Jaboticabal, SP, BrazilAmer Soc MicrobiologyInst Natl Rech AgronInst PasteurUniversidade Estadual Paulista (Unesp)Vilas-Boas, G.Sanchis, VLereclus, D.Lemos, MVFBourguet, D.2014-05-20T13:13:01Z2014-05-20T13:13:01Z2002-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1414-1424application/pdfhttp://dx.doi.org/10.1128/AEM.68.3.1414-1424.2002Applied and Environmental Microbiology. Washington: Amer Soc Microbiology, v. 68, n. 3, p. 1414-1424, 2002.0099-2240http://hdl.handle.net/11449/93110.1128/AEM.68.3.1414-1424.2002WOS:000174206200051WOS000174206200051.pdf7179273060624761Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengApplied and Environmental Microbiology3.6331,684info:eu-repo/semantics/openAccess2024-06-06T13:05:09Zoai:repositorio.unesp.br:11449/931Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:19:43.348276Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Genetic differentiation between sympatric populations of Bacillus cereus and Bacillus thuringiensis |
title |
Genetic differentiation between sympatric populations of Bacillus cereus and Bacillus thuringiensis |
spellingShingle |
Genetic differentiation between sympatric populations of Bacillus cereus and Bacillus thuringiensis Vilas-Boas, G. |
title_short |
Genetic differentiation between sympatric populations of Bacillus cereus and Bacillus thuringiensis |
title_full |
Genetic differentiation between sympatric populations of Bacillus cereus and Bacillus thuringiensis |
title_fullStr |
Genetic differentiation between sympatric populations of Bacillus cereus and Bacillus thuringiensis |
title_full_unstemmed |
Genetic differentiation between sympatric populations of Bacillus cereus and Bacillus thuringiensis |
title_sort |
Genetic differentiation between sympatric populations of Bacillus cereus and Bacillus thuringiensis |
author |
Vilas-Boas, G. |
author_facet |
Vilas-Boas, G. Sanchis, V Lereclus, D. Lemos, MVF Bourguet, D. |
author_role |
author |
author2 |
Sanchis, V Lereclus, D. Lemos, MVF Bourguet, D. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Inst Natl Rech Agron Inst Pasteur Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Vilas-Boas, G. Sanchis, V Lereclus, D. Lemos, MVF Bourguet, D. |
description |
Little is known about genetic exchanges in natural populations of bacteria of the spore-forming Bacillus cereus group, because no population genetics studies have been performed with local sympatric populations. We isolated strains of Bacillus thuringiensis and B. cereus from small samples of soil collected at the same time from two separate geographical sites, one within the forest and the other at the edge of the forest. A total of 100 B. cercus and 98 B. thuringiensis strains were isolated and characterized by electrophoresis to determine allelic composition at nine enzymatic loci. We observed genetic differentiation between populations of B. cereus and B. thuringiensis. Populations of a given Bacillus species-B. thuringiensis or B. cereus-were genetically more similar to each other than to populations of the other Bacillus species. Hemolytic activity provided further evidence of this genetic divergence, which remained evident even if putative clones were removed from the data set. Our results suggest that the rate of gene flow was higher between strains of the same species, but that exchanges between B. cereus and B. thuringiensis were nonetheless possible. Linkage disequilibrium analysis revealed sufficient recombination for B. cereus populations to be considered panmictic units. In B. thuringiensis, the balance between clonal proliferation and recombination seemed to depend on location. Overall, our data indicate that it is not important for risk assessment purposes to determine whether B. cereus and B. thuringiensis belong to a single or two species. Assessment of the biosafety of pest control based on B. thuringiensis requires evaluation of the extent of genetic exchange between strains in realistic natural conditions. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-03-01 2014-05-20T13:13:01Z 2014-05-20T13:13:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1128/AEM.68.3.1414-1424.2002 Applied and Environmental Microbiology. Washington: Amer Soc Microbiology, v. 68, n. 3, p. 1414-1424, 2002. 0099-2240 http://hdl.handle.net/11449/931 10.1128/AEM.68.3.1414-1424.2002 WOS:000174206200051 WOS000174206200051.pdf 7179273060624761 |
url |
http://dx.doi.org/10.1128/AEM.68.3.1414-1424.2002 http://hdl.handle.net/11449/931 |
identifier_str_mv |
Applied and Environmental Microbiology. Washington: Amer Soc Microbiology, v. 68, n. 3, p. 1414-1424, 2002. 0099-2240 10.1128/AEM.68.3.1414-1424.2002 WOS:000174206200051 WOS000174206200051.pdf 7179273060624761 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Applied and Environmental Microbiology 3.633 1,684 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1414-1424 application/pdf |
dc.publisher.none.fl_str_mv |
Amer Soc Microbiology |
publisher.none.fl_str_mv |
Amer Soc Microbiology |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129188501127168 |