Exploring the Galleria mellonella model to study antifungal photodynamic therapy
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.pdpdt.2019.05.010 http://hdl.handle.net/11449/189183 |
Resumo: | Background: Antimicrobial photodynamic therapy (aPDT)shows antimicrobial activity on yeast of the genus Candida. In aPDT, the depth at which the light penetrates the tissue is extremely important for the elaboration of the treatment. The aim of this study was to evaluate the action of aPDT on experimental candidiasis and the laser impact in the tissue using Galleria mellonella as the infection model. Methods: G. mellonella larvae were infected with different Candida albicans strains. After 30 min, they were treated with methylene blue-mediated aPDT and a low intensity laser (660 nm). The larvae were incubated at 37 °C for seven days and monitored daily to determine the survival curve, using the Log-rank test (Mantel Cox). To evaluate the distribution of the laser as well as its depth of action in the larva body, the Interactive 3D surface PLOT of Image J was used. The effects of aPDT on the immune system were also evaluated by the quantification of hemocytes in the hemolymph of G. mellonella after 6 h of Candida infection (ANOVA and Tukey's test). Results: In both the ATCC 18,804 strain and the C. albicans clinical strain 17, aPDT prolonged the survival of the infected G. mellonella larvae by a lethal fungal dose. There was a statistically significant difference between the aPDT and the control groups in the ATCC strain (P = 0.0056). The depth of laser action in the insect body without the photosensitizer was 2.5 mm and 2.4 mm from the cuticle of the larva with the photosensitizer. In the larvae, a uniform distribution of light occurred along 32% of the body length for the group without the photosensitizer and in 39.5% for the group with the photosensitizer. In the immunological analysis, the infection by C. albicans ATCC 18,804 in G. mellonella led to a reduction in the number of hemocytes in the hemolymph. The aPDT and laser treatment induced a slight increase in the number of hemocytes. Conclusion: Both aPDT and laser treatment positively influenced the treatment of experimental candidiasis. G. mellonella larvae were a useful model for the study of light tissue penetration in antimicrobial photodynamic therapy. |
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Exploring the Galleria mellonella model to study antifungal photodynamic therapyAntimicrobial photodynamic TherapyCandida albicansInvertebratePhotodynamic therapyBackground: Antimicrobial photodynamic therapy (aPDT)shows antimicrobial activity on yeast of the genus Candida. In aPDT, the depth at which the light penetrates the tissue is extremely important for the elaboration of the treatment. The aim of this study was to evaluate the action of aPDT on experimental candidiasis and the laser impact in the tissue using Galleria mellonella as the infection model. Methods: G. mellonella larvae were infected with different Candida albicans strains. After 30 min, they were treated with methylene blue-mediated aPDT and a low intensity laser (660 nm). The larvae were incubated at 37 °C for seven days and monitored daily to determine the survival curve, using the Log-rank test (Mantel Cox). To evaluate the distribution of the laser as well as its depth of action in the larva body, the Interactive 3D surface PLOT of Image J was used. The effects of aPDT on the immune system were also evaluated by the quantification of hemocytes in the hemolymph of G. mellonella after 6 h of Candida infection (ANOVA and Tukey's test). Results: In both the ATCC 18,804 strain and the C. albicans clinical strain 17, aPDT prolonged the survival of the infected G. mellonella larvae by a lethal fungal dose. There was a statistically significant difference between the aPDT and the control groups in the ATCC strain (P = 0.0056). The depth of laser action in the insect body without the photosensitizer was 2.5 mm and 2.4 mm from the cuticle of the larva with the photosensitizer. In the larvae, a uniform distribution of light occurred along 32% of the body length for the group without the photosensitizer and in 39.5% for the group with the photosensitizer. In the immunological analysis, the infection by C. albicans ATCC 18,804 in G. mellonella led to a reduction in the number of hemocytes in the hemolymph. The aPDT and laser treatment induced a slight increase in the number of hemocytes. Conclusion: Both aPDT and laser treatment positively influenced the treatment of experimental candidiasis. G. mellonella larvae were a useful model for the study of light tissue penetration in antimicrobial photodynamic therapy.Department of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology (ICT) São José dos Campos CampusSão Leopoldo Mandic Dental Research CenterDepartment of Biosciences and Oral Diagnosis São Paulo State University (Unesp) Institute of Science and Technology (ICT) São José dos Campos CampusUniversidade Estadual Paulista (Unesp)São Leopoldo Mandic Dental Research CenterFigueiredo-Godoi, Lívia Mara Alves [UNESP]Menezes, Raquel Telles [UNESP]Carvalho, Jéssica Shiotani [UNESP]Garcia, Maíra Terra [UNESP]Segundo, Aguinaldo GarcezJorge, Antonio Olavo Cardoso [UNESP]Junqueira, Juliana Campos [UNESP]2019-10-06T16:32:33Z2019-10-06T16:32:33Z2019-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article66-73http://dx.doi.org/10.1016/j.pdpdt.2019.05.010Photodiagnosis and Photodynamic Therapy, v. 27, p. 66-73.1873-15971572-1000http://hdl.handle.net/11449/18918310.1016/j.pdpdt.2019.05.0102-s2.0-85066436365Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPhotodiagnosis and Photodynamic Therapyinfo:eu-repo/semantics/openAccess2021-10-23T04:16:07Zoai:repositorio.unesp.br:11449/189183Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:51:48.747817Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Exploring the Galleria mellonella model to study antifungal photodynamic therapy |
title |
Exploring the Galleria mellonella model to study antifungal photodynamic therapy |
spellingShingle |
Exploring the Galleria mellonella model to study antifungal photodynamic therapy Figueiredo-Godoi, Lívia Mara Alves [UNESP] Antimicrobial photodynamic Therapy Candida albicans Invertebrate Photodynamic therapy |
title_short |
Exploring the Galleria mellonella model to study antifungal photodynamic therapy |
title_full |
Exploring the Galleria mellonella model to study antifungal photodynamic therapy |
title_fullStr |
Exploring the Galleria mellonella model to study antifungal photodynamic therapy |
title_full_unstemmed |
Exploring the Galleria mellonella model to study antifungal photodynamic therapy |
title_sort |
Exploring the Galleria mellonella model to study antifungal photodynamic therapy |
author |
Figueiredo-Godoi, Lívia Mara Alves [UNESP] |
author_facet |
Figueiredo-Godoi, Lívia Mara Alves [UNESP] Menezes, Raquel Telles [UNESP] Carvalho, Jéssica Shiotani [UNESP] Garcia, Maíra Terra [UNESP] Segundo, Aguinaldo Garcez Jorge, Antonio Olavo Cardoso [UNESP] Junqueira, Juliana Campos [UNESP] |
author_role |
author |
author2 |
Menezes, Raquel Telles [UNESP] Carvalho, Jéssica Shiotani [UNESP] Garcia, Maíra Terra [UNESP] Segundo, Aguinaldo Garcez Jorge, Antonio Olavo Cardoso [UNESP] Junqueira, Juliana Campos [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) São Leopoldo Mandic Dental Research Center |
dc.contributor.author.fl_str_mv |
Figueiredo-Godoi, Lívia Mara Alves [UNESP] Menezes, Raquel Telles [UNESP] Carvalho, Jéssica Shiotani [UNESP] Garcia, Maíra Terra [UNESP] Segundo, Aguinaldo Garcez Jorge, Antonio Olavo Cardoso [UNESP] Junqueira, Juliana Campos [UNESP] |
dc.subject.por.fl_str_mv |
Antimicrobial photodynamic Therapy Candida albicans Invertebrate Photodynamic therapy |
topic |
Antimicrobial photodynamic Therapy Candida albicans Invertebrate Photodynamic therapy |
description |
Background: Antimicrobial photodynamic therapy (aPDT)shows antimicrobial activity on yeast of the genus Candida. In aPDT, the depth at which the light penetrates the tissue is extremely important for the elaboration of the treatment. The aim of this study was to evaluate the action of aPDT on experimental candidiasis and the laser impact in the tissue using Galleria mellonella as the infection model. Methods: G. mellonella larvae were infected with different Candida albicans strains. After 30 min, they were treated with methylene blue-mediated aPDT and a low intensity laser (660 nm). The larvae were incubated at 37 °C for seven days and monitored daily to determine the survival curve, using the Log-rank test (Mantel Cox). To evaluate the distribution of the laser as well as its depth of action in the larva body, the Interactive 3D surface PLOT of Image J was used. The effects of aPDT on the immune system were also evaluated by the quantification of hemocytes in the hemolymph of G. mellonella after 6 h of Candida infection (ANOVA and Tukey's test). Results: In both the ATCC 18,804 strain and the C. albicans clinical strain 17, aPDT prolonged the survival of the infected G. mellonella larvae by a lethal fungal dose. There was a statistically significant difference between the aPDT and the control groups in the ATCC strain (P = 0.0056). The depth of laser action in the insect body without the photosensitizer was 2.5 mm and 2.4 mm from the cuticle of the larva with the photosensitizer. In the larvae, a uniform distribution of light occurred along 32% of the body length for the group without the photosensitizer and in 39.5% for the group with the photosensitizer. In the immunological analysis, the infection by C. albicans ATCC 18,804 in G. mellonella led to a reduction in the number of hemocytes in the hemolymph. The aPDT and laser treatment induced a slight increase in the number of hemocytes. Conclusion: Both aPDT and laser treatment positively influenced the treatment of experimental candidiasis. G. mellonella larvae were a useful model for the study of light tissue penetration in antimicrobial photodynamic therapy. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:32:33Z 2019-10-06T16:32:33Z 2019-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.pdpdt.2019.05.010 Photodiagnosis and Photodynamic Therapy, v. 27, p. 66-73. 1873-1597 1572-1000 http://hdl.handle.net/11449/189183 10.1016/j.pdpdt.2019.05.010 2-s2.0-85066436365 |
url |
http://dx.doi.org/10.1016/j.pdpdt.2019.05.010 http://hdl.handle.net/11449/189183 |
identifier_str_mv |
Photodiagnosis and Photodynamic Therapy, v. 27, p. 66-73. 1873-1597 1572-1000 10.1016/j.pdpdt.2019.05.010 2-s2.0-85066436365 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Photodiagnosis and Photodynamic Therapy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
66-73 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129259172003840 |