Cost-effective lactic acid production by fermentation of agro-industrial residues

• Autor(a) principal: Carpinelli Macedo, João Victor [UNESP]
• Data de Publicação: 2020
• Outros Autores: de Barros Ranke, Fabiane Fernanda [UNESP], Escaramboni, Bruna [UNESP], Campioni, Tania Sila [UNESP], Fernández Núñez, Eutimio Gustavo, de Oliva Neto, Pedro [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.bcab.2020.101706; http://hdl.handle.net/11449/199238
• topic: Agro-industrial residues; Biological treatment; Bioprocess; Fermentation; Lactic acid; Lactobacillus

The substrate cost is a hurdle in the lactic acid (LA) manufacturing by microbial fermentations. Thus, this work aimed to optimize the production of LA by Lactobacillus amylovorus and Lactobacillus acidophilus in a culture medium containing agro-industrial wastes, cassava bagasse (CB, 6–18%, w/v) and corn steep liquor (CSL, 4–12%, v/v). Initially, the optimal medium composition was defined; different fermentation times were also assessed. CB was used in natura or hydrolyzed by Rhizopus oligosporus' amylase. In a batch process without pH control, the LA maximum concentration (31.6 g/L) and productivity (0.11 [Formula presented]) were obtained by L. amylovorus using 14% (w/v) hydrolyzed CB and 10% (v/v) CSL. However, when a Fed-batch process was performed with pH control and using the optimized culture medium composition, high volumetric productivity (0.46 [Formula presented]) was achieved. The optimized medium's cost is 0.05 US$/L. These findings suggest a promising bioprocess for LA manufacturing from agro-industrial wastes.