Galectin-3 is a key hepatoprotective molecule against the deleterious effect of cisplatin

Detalhes bibliográficos
Autor(a) principal: Santos, Diego D. [UNESP]
Data de Publicação: 2023
Outros Autores: Sasso, Gisela R.S., Belote, Nycole M., da Silva, Rafael André [UNESP], Lice, Izabella, Correia-Silva, Rebeca D., Borges, Fernanda T., Carbonel, Adriana A.F., Gil, Cristiane D. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.lfs.2023.121505
http://hdl.handle.net/11449/246849
Resumo: Aims: Evaluate the role of galectin-3 in the liver using an acute model of cisplatin-induced toxicity. Material and methods: Modified citrus pectin (MCP) treatment was used to inhibit galectin-3. Rats were distributed into four groups: SHAM, CIS, MCP and MCP + CIS. On days 1–7, animals were treated by oral gavage with 100 mg/kg/day of MCP (MCP and MCP + CIS groups). On days 8, 9 and 10, animals received intraperitoneal injection of 10 mg/kg/day of cisplatin (CIS and MCP + CIS groups) or saline (SHAM and MCP groups). Key findings: Cisplatin administration caused a marked increase in hepatic leukocyte influx and liver degeneration, and promoted reactive oxygen species production and STAT3 activation in hepatocytes. Plasma levels of cytokines (IL-6, IL-10), and hepatic toxicity biomarkers (hepatic arginase 1, α-glutathione S-transferase, sorbitol dehydrogenase) were also elevated. Decreased galectin-3 levels in the livers of animals in the MCP + CIS group were also associated with increased hepatic levels of malondialdehyde and mitochondrial respiratory complex I. Animals in the MCP + CIS group also exhibited increased plasma levels of IL-1β, TNF-α, and aspartate transaminase 1. Furthermore, MCP therapy efficiently antagonized hepatic galectin-9 in liver, but not galectin-1, the latter of which was increased. Significance: Reduction of the endogenous levels of galectin-3 in hepatocytes favors the process of cell death and increases oxidative stress in the acute model of cisplatin-induced toxicity.
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spelling Galectin-3 is a key hepatoprotective molecule against the deleterious effect of cisplatinHepatotoxicityInflammationLipid peroxidationMitochondriaModified citrus pectinReactive oxygen speciesAims: Evaluate the role of galectin-3 in the liver using an acute model of cisplatin-induced toxicity. Material and methods: Modified citrus pectin (MCP) treatment was used to inhibit galectin-3. Rats were distributed into four groups: SHAM, CIS, MCP and MCP + CIS. On days 1–7, animals were treated by oral gavage with 100 mg/kg/day of MCP (MCP and MCP + CIS groups). On days 8, 9 and 10, animals received intraperitoneal injection of 10 mg/kg/day of cisplatin (CIS and MCP + CIS groups) or saline (SHAM and MCP groups). Key findings: Cisplatin administration caused a marked increase in hepatic leukocyte influx and liver degeneration, and promoted reactive oxygen species production and STAT3 activation in hepatocytes. Plasma levels of cytokines (IL-6, IL-10), and hepatic toxicity biomarkers (hepatic arginase 1, α-glutathione S-transferase, sorbitol dehydrogenase) were also elevated. Decreased galectin-3 levels in the livers of animals in the MCP + CIS group were also associated with increased hepatic levels of malondialdehyde and mitochondrial respiratory complex I. Animals in the MCP + CIS group also exhibited increased plasma levels of IL-1β, TNF-α, and aspartate transaminase 1. Furthermore, MCP therapy efficiently antagonized hepatic galectin-9 in liver, but not galectin-1, the latter of which was increased. Significance: Reduction of the endogenous levels of galectin-3 in hepatocytes favors the process of cell death and increases oxidative stress in the acute model of cisplatin-induced toxicity.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Biosciences Graduate Program Institute of Biosciences Letters and Exact Sciences Universidade Estadual Paulista (UNESP), SPStructural and Functional Biology Graduate Program Universidade Federal de São Paulo (UNIFESP), SPDepartment of Medicine Nephrology Division Universidade Federal de São Paulo (UNIFESP), SPBiosciences Graduate Program Institute of Biosciences Letters and Exact Sciences Universidade Estadual Paulista (UNESP), SPCAPES: 001FAPESP: 20/03565-2Universidade Estadual Paulista (UNESP)Universidade Federal de São Paulo (UNIFESP)Santos, Diego D. [UNESP]Sasso, Gisela R.S.Belote, Nycole M.da Silva, Rafael André [UNESP]Lice, IzabellaCorreia-Silva, Rebeca D.Borges, Fernanda T.Carbonel, Adriana A.F.Gil, Cristiane D. [UNESP]2023-07-29T12:52:13Z2023-07-29T12:52:13Z2023-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.lfs.2023.121505Life Sciences, v. 318.1879-06310024-3205http://hdl.handle.net/11449/24684910.1016/j.lfs.2023.1215052-s2.0-85148355507Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLife Sciencesinfo:eu-repo/semantics/openAccess2023-07-29T12:52:13Zoai:repositorio.unesp.br:11449/246849Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:09:27.736556Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Galectin-3 is a key hepatoprotective molecule against the deleterious effect of cisplatin
title Galectin-3 is a key hepatoprotective molecule against the deleterious effect of cisplatin
spellingShingle Galectin-3 is a key hepatoprotective molecule against the deleterious effect of cisplatin
Santos, Diego D. [UNESP]
Hepatotoxicity
Inflammation
Lipid peroxidation
Mitochondria
Modified citrus pectin
Reactive oxygen species
title_short Galectin-3 is a key hepatoprotective molecule against the deleterious effect of cisplatin
title_full Galectin-3 is a key hepatoprotective molecule against the deleterious effect of cisplatin
title_fullStr Galectin-3 is a key hepatoprotective molecule against the deleterious effect of cisplatin
title_full_unstemmed Galectin-3 is a key hepatoprotective molecule against the deleterious effect of cisplatin
title_sort Galectin-3 is a key hepatoprotective molecule against the deleterious effect of cisplatin
author Santos, Diego D. [UNESP]
author_facet Santos, Diego D. [UNESP]
Sasso, Gisela R.S.
Belote, Nycole M.
da Silva, Rafael André [UNESP]
Lice, Izabella
Correia-Silva, Rebeca D.
Borges, Fernanda T.
Carbonel, Adriana A.F.
Gil, Cristiane D. [UNESP]
author_role author
author2 Sasso, Gisela R.S.
Belote, Nycole M.
da Silva, Rafael André [UNESP]
Lice, Izabella
Correia-Silva, Rebeca D.
Borges, Fernanda T.
Carbonel, Adriana A.F.
Gil, Cristiane D. [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Federal de São Paulo (UNIFESP)
dc.contributor.author.fl_str_mv Santos, Diego D. [UNESP]
Sasso, Gisela R.S.
Belote, Nycole M.
da Silva, Rafael André [UNESP]
Lice, Izabella
Correia-Silva, Rebeca D.
Borges, Fernanda T.
Carbonel, Adriana A.F.
Gil, Cristiane D. [UNESP]
dc.subject.por.fl_str_mv Hepatotoxicity
Inflammation
Lipid peroxidation
Mitochondria
Modified citrus pectin
Reactive oxygen species
topic Hepatotoxicity
Inflammation
Lipid peroxidation
Mitochondria
Modified citrus pectin
Reactive oxygen species
description Aims: Evaluate the role of galectin-3 in the liver using an acute model of cisplatin-induced toxicity. Material and methods: Modified citrus pectin (MCP) treatment was used to inhibit galectin-3. Rats were distributed into four groups: SHAM, CIS, MCP and MCP + CIS. On days 1–7, animals were treated by oral gavage with 100 mg/kg/day of MCP (MCP and MCP + CIS groups). On days 8, 9 and 10, animals received intraperitoneal injection of 10 mg/kg/day of cisplatin (CIS and MCP + CIS groups) or saline (SHAM and MCP groups). Key findings: Cisplatin administration caused a marked increase in hepatic leukocyte influx and liver degeneration, and promoted reactive oxygen species production and STAT3 activation in hepatocytes. Plasma levels of cytokines (IL-6, IL-10), and hepatic toxicity biomarkers (hepatic arginase 1, α-glutathione S-transferase, sorbitol dehydrogenase) were also elevated. Decreased galectin-3 levels in the livers of animals in the MCP + CIS group were also associated with increased hepatic levels of malondialdehyde and mitochondrial respiratory complex I. Animals in the MCP + CIS group also exhibited increased plasma levels of IL-1β, TNF-α, and aspartate transaminase 1. Furthermore, MCP therapy efficiently antagonized hepatic galectin-9 in liver, but not galectin-1, the latter of which was increased. Significance: Reduction of the endogenous levels of galectin-3 in hepatocytes favors the process of cell death and increases oxidative stress in the acute model of cisplatin-induced toxicity.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T12:52:13Z
2023-07-29T12:52:13Z
2023-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.lfs.2023.121505
Life Sciences, v. 318.
1879-0631
0024-3205
http://hdl.handle.net/11449/246849
10.1016/j.lfs.2023.121505
2-s2.0-85148355507
url http://dx.doi.org/10.1016/j.lfs.2023.121505
http://hdl.handle.net/11449/246849
identifier_str_mv Life Sciences, v. 318.
1879-0631
0024-3205
10.1016/j.lfs.2023.121505
2-s2.0-85148355507
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Life Sciences
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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