In utero protein restriction causes growth delay and alters sperm parameters in adult male rats
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1477-7827-9-94 http://hdl.handle.net/11449/18733 |
Resumo: | Background: Recent studies have supported the concept of fetal programming which suggests that during the intrauterine development the fetus may be programmed to develop diseases in adulthood. The possible effects of in utero protein restriction on sexual development of rat male offspring were evaluated in the present study.Methods: Pregnant Wistar rats were divided into two experimental groups: one group treated with standard chow (SC, n = 8, 17% protein) and the other group treated with hypoproteic chow (HC, n = 10, 6% protein) throughout gestation. After gestation the two experimental groups received standard chow. To evaluate the possible late reproductive effects of in utero protein restriction, the male offspring of both groups were assessed at different phases of sexual development: prepubertal (30 days old); peripubertal (60 days old); adult (90 days old). Student's t test and Mann-Whitney test were utilized. Differences were considered significant when p < 0.05.Results: We found that in utero protein restriction reduced the body weight of male pups on the first postnatal day and during the different sexual development phases (prepubertal, peripubertal and adult). During adulthood, Sertoli cell number, sperm motility and sperm counts in the testis and epididymal cauda were also reduced in HC. Furthermore, the numbers of sperm presenting morphological abnormalities and cytoplasmic drop retention were higher in HC.Conclusions: In conclusion, in utero protein restriction, under these experimental conditions, causes growth delay and alters male reproductive-system programming in rats, suggesting impairment of sperm quality in adulthood. |
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In utero protein restriction causes growth delay and alters sperm parameters in adult male ratsBackground: Recent studies have supported the concept of fetal programming which suggests that during the intrauterine development the fetus may be programmed to develop diseases in adulthood. The possible effects of in utero protein restriction on sexual development of rat male offspring were evaluated in the present study.Methods: Pregnant Wistar rats were divided into two experimental groups: one group treated with standard chow (SC, n = 8, 17% protein) and the other group treated with hypoproteic chow (HC, n = 10, 6% protein) throughout gestation. After gestation the two experimental groups received standard chow. To evaluate the possible late reproductive effects of in utero protein restriction, the male offspring of both groups were assessed at different phases of sexual development: prepubertal (30 days old); peripubertal (60 days old); adult (90 days old). Student's t test and Mann-Whitney test were utilized. Differences were considered significant when p < 0.05.Results: We found that in utero protein restriction reduced the body weight of male pups on the first postnatal day and during the different sexual development phases (prepubertal, peripubertal and adult). During adulthood, Sertoli cell number, sperm motility and sperm counts in the testis and epididymal cauda were also reduced in HC. Furthermore, the numbers of sperm presenting morphological abnormalities and cytoplasmic drop retention were higher in HC.Conclusions: In conclusion, in utero protein restriction, under these experimental conditions, causes growth delay and alters male reproductive-system programming in rats, suggesting impairment of sperm quality in adulthood.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, UNESP, Inst Biosci, Dept Morphol, Botucatu, SP, BrazilUniv Estadual Campinas, Inst Biol, Grad Program Cell & Struct Biol, Campinas, SP, BrazilUniv São Paulo, Sch Dent, Dept Morphol Stomatol & Physiol, BR-14049 Ribeirao Preto, SP, BrazilUniv Estadual Paulista, UNESP, Inst Biosci, Dept Morphol, Botucatu, SP, BrazilFAPESP: 07/57375-5FAPESP: 08/51374-0Biomed Central Ltd.Universidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)Toledo, Fabiola C. [UNESP]Perobelli, Juliana E. [UNESP]Pedrosa, Flavia P. C. [UNESP]Anselmo-Franci, Janete A.Kempinas, Wilma D. G. [UNESP]2014-05-20T13:52:26Z2014-05-20T13:52:26Z2011-06-24info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article9application/pdfhttp://dx.doi.org/10.1186/1477-7827-9-94Reproductive Biology and Endocrinology. London: Biomed Central Ltd., v. 9, p. 9, 2011.1477-7827http://hdl.handle.net/11449/1873310.1186/1477-7827-9-94WOS:000292939400001WOS000292939400001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengReproductive Biology and Endocrinology2.8521,203info:eu-repo/semantics/openAccess2023-10-22T06:07:43Zoai:repositorio.unesp.br:11449/18733Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-22T06:07:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In utero protein restriction causes growth delay and alters sperm parameters in adult male rats |
title |
In utero protein restriction causes growth delay and alters sperm parameters in adult male rats |
spellingShingle |
In utero protein restriction causes growth delay and alters sperm parameters in adult male rats Toledo, Fabiola C. [UNESP] |
title_short |
In utero protein restriction causes growth delay and alters sperm parameters in adult male rats |
title_full |
In utero protein restriction causes growth delay and alters sperm parameters in adult male rats |
title_fullStr |
In utero protein restriction causes growth delay and alters sperm parameters in adult male rats |
title_full_unstemmed |
In utero protein restriction causes growth delay and alters sperm parameters in adult male rats |
title_sort |
In utero protein restriction causes growth delay and alters sperm parameters in adult male rats |
author |
Toledo, Fabiola C. [UNESP] |
author_facet |
Toledo, Fabiola C. [UNESP] Perobelli, Juliana E. [UNESP] Pedrosa, Flavia P. C. [UNESP] Anselmo-Franci, Janete A. Kempinas, Wilma D. G. [UNESP] |
author_role |
author |
author2 |
Perobelli, Juliana E. [UNESP] Pedrosa, Flavia P. C. [UNESP] Anselmo-Franci, Janete A. Kempinas, Wilma D. G. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Toledo, Fabiola C. [UNESP] Perobelli, Juliana E. [UNESP] Pedrosa, Flavia P. C. [UNESP] Anselmo-Franci, Janete A. Kempinas, Wilma D. G. [UNESP] |
description |
Background: Recent studies have supported the concept of fetal programming which suggests that during the intrauterine development the fetus may be programmed to develop diseases in adulthood. The possible effects of in utero protein restriction on sexual development of rat male offspring were evaluated in the present study.Methods: Pregnant Wistar rats were divided into two experimental groups: one group treated with standard chow (SC, n = 8, 17% protein) and the other group treated with hypoproteic chow (HC, n = 10, 6% protein) throughout gestation. After gestation the two experimental groups received standard chow. To evaluate the possible late reproductive effects of in utero protein restriction, the male offspring of both groups were assessed at different phases of sexual development: prepubertal (30 days old); peripubertal (60 days old); adult (90 days old). Student's t test and Mann-Whitney test were utilized. Differences were considered significant when p < 0.05.Results: We found that in utero protein restriction reduced the body weight of male pups on the first postnatal day and during the different sexual development phases (prepubertal, peripubertal and adult). During adulthood, Sertoli cell number, sperm motility and sperm counts in the testis and epididymal cauda were also reduced in HC. Furthermore, the numbers of sperm presenting morphological abnormalities and cytoplasmic drop retention were higher in HC.Conclusions: In conclusion, in utero protein restriction, under these experimental conditions, causes growth delay and alters male reproductive-system programming in rats, suggesting impairment of sperm quality in adulthood. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-06-24 2014-05-20T13:52:26Z 2014-05-20T13:52:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1477-7827-9-94 Reproductive Biology and Endocrinology. London: Biomed Central Ltd., v. 9, p. 9, 2011. 1477-7827 http://hdl.handle.net/11449/18733 10.1186/1477-7827-9-94 WOS:000292939400001 WOS000292939400001.pdf |
url |
http://dx.doi.org/10.1186/1477-7827-9-94 http://hdl.handle.net/11449/18733 |
identifier_str_mv |
Reproductive Biology and Endocrinology. London: Biomed Central Ltd., v. 9, p. 9, 2011. 1477-7827 10.1186/1477-7827-9-94 WOS:000292939400001 WOS000292939400001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Reproductive Biology and Endocrinology 2.852 1,203 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
9 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803046102545465344 |