Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytes
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/385 http://hdl.handle.net/11449/69342 |
Resumo: | It is known that the invasin molecule of Yersinia pseudotuberculosis stimulates human peripheral B cells in vitro. In this work we evaluated the in vivo role of invasin as polyclonal activator of B lymphocytes in the mouse experimental model, by comparing strains of Y. pseudotuberculosis expressing invasin and isogenic inv mutants. Swiss mice were infected intravenously with two strains expressing invasin (YpIII pIB1 and an isogenic virulence plasmid-cured strain, YpIII) and with two invasin mutant strains (Yp100 pIB1 and Yp100, plasmid-cured). Spleen cells were sampled on days 7, 14, 21 and 28 after infection. Immunoglobulin (Ig)-secreting spleen cells were detected by protein A plaque assay and specific antibodies were detected in sera by ELISA. The virulent strain YPIII pIB1 (wild type) did not provoke polyclonal activation of B lymphocytes in vivo. In general, fewer Ig-secreting spleen cells of all isotypes were found in the infected animals than in the control animals. Specific IgG antibodies were detected in the sera of animals infected with all strains. The peak response occurred on the 21 st day post-infection, and the Yp100 strain provoked the highest level of these antibodies. We concluded that invasin is not a polyclonal activator of murine B cells. |
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Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytesB lymphocytesInvasinPolyclonal activationSpecific antibodiesYersinia pseudotuberculosisimmunoglobulininvasinprotein Aanalytic methodanimal experimentB lymphocyte activationbacterial straincomparative studycontrolled studyenzyme linked immunosorbent assayfemalemousenonhumanspleen cellIt is known that the invasin molecule of Yersinia pseudotuberculosis stimulates human peripheral B cells in vitro. In this work we evaluated the in vivo role of invasin as polyclonal activator of B lymphocytes in the mouse experimental model, by comparing strains of Y. pseudotuberculosis expressing invasin and isogenic inv mutants. Swiss mice were infected intravenously with two strains expressing invasin (YpIII pIB1 and an isogenic virulence plasmid-cured strain, YpIII) and with two invasin mutant strains (Yp100 pIB1 and Yp100, plasmid-cured). Spleen cells were sampled on days 7, 14, 21 and 28 after infection. Immunoglobulin (Ig)-secreting spleen cells were detected by protein A plaque assay and specific antibodies were detected in sera by ELISA. The virulent strain YPIII pIB1 (wild type) did not provoke polyclonal activation of B lymphocytes in vivo. In general, fewer Ig-secreting spleen cells of all isotypes were found in the infected animals than in the control animals. Specific IgG antibodies were detected in the sera of animals infected with all strains. The peak response occurred on the 21 st day post-infection, and the Yp100 strain provoked the highest level of these antibodies. We concluded that invasin is not a polyclonal activator of murine B cells.Department of Biological Sciences School of Pharmaceutical Sciences São Paulo State University, UNESP, Araraquara, SPDepartamento de Ciências Biológicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, UNESP, Rodovia Araraquara-Jaú, km 1, CEP: 14801-902 - Araraquara - SPDepartment of Biological Sciences School of Pharmaceutical Sciences São Paulo State University, UNESP, Araraquara, SPDepartamento de Ciências Biológicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, UNESP, Rodovia Araraquara-Jaú, km 1, CEP: 14801-902 - Araraquara - SPUniversidade Estadual Paulista (Unesp)De Medeiros, Beatriz Maria Machado [UNESP]Cangiani, E. E. [UNESP]Higuti, L. [UNESP]Satomi, L. C. [UNESP]Souza, C. D. [UNESP]Silva, A. R C [UNESP]Maia, J. M L [UNESP]Falcão, D. P. [UNESP]2014-05-27T11:22:05Z2014-05-27T11:22:05Z2006-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article221-227application/pdfhttp://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/385Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 27, n. 3, p. 221-227, 2006.1808-4532http://hdl.handle.net/11449/693422-s2.0-349488737912-s2.0-34948873791.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista de Ciências Farmacêuticas Básica e Aplicada0,131info:eu-repo/semantics/openAccess2024-06-24T13:07:28Zoai:repositorio.unesp.br:11449/69342Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:20:54.077940Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytes |
title |
Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytes |
spellingShingle |
Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytes De Medeiros, Beatriz Maria Machado [UNESP] B lymphocytes Invasin Polyclonal activation Specific antibodies Yersinia pseudotuberculosis immunoglobulin invasin protein A analytic method animal experiment B lymphocyte activation bacterial strain comparative study controlled study enzyme linked immunosorbent assay female mouse nonhuman spleen cell |
title_short |
Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytes |
title_full |
Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytes |
title_fullStr |
Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytes |
title_full_unstemmed |
Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytes |
title_sort |
Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytes |
author |
De Medeiros, Beatriz Maria Machado [UNESP] |
author_facet |
De Medeiros, Beatriz Maria Machado [UNESP] Cangiani, E. E. [UNESP] Higuti, L. [UNESP] Satomi, L. C. [UNESP] Souza, C. D. [UNESP] Silva, A. R C [UNESP] Maia, J. M L [UNESP] Falcão, D. P. [UNESP] |
author_role |
author |
author2 |
Cangiani, E. E. [UNESP] Higuti, L. [UNESP] Satomi, L. C. [UNESP] Souza, C. D. [UNESP] Silva, A. R C [UNESP] Maia, J. M L [UNESP] Falcão, D. P. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
De Medeiros, Beatriz Maria Machado [UNESP] Cangiani, E. E. [UNESP] Higuti, L. [UNESP] Satomi, L. C. [UNESP] Souza, C. D. [UNESP] Silva, A. R C [UNESP] Maia, J. M L [UNESP] Falcão, D. P. [UNESP] |
dc.subject.por.fl_str_mv |
B lymphocytes Invasin Polyclonal activation Specific antibodies Yersinia pseudotuberculosis immunoglobulin invasin protein A analytic method animal experiment B lymphocyte activation bacterial strain comparative study controlled study enzyme linked immunosorbent assay female mouse nonhuman spleen cell |
topic |
B lymphocytes Invasin Polyclonal activation Specific antibodies Yersinia pseudotuberculosis immunoglobulin invasin protein A analytic method animal experiment B lymphocyte activation bacterial strain comparative study controlled study enzyme linked immunosorbent assay female mouse nonhuman spleen cell |
description |
It is known that the invasin molecule of Yersinia pseudotuberculosis stimulates human peripheral B cells in vitro. In this work we evaluated the in vivo role of invasin as polyclonal activator of B lymphocytes in the mouse experimental model, by comparing strains of Y. pseudotuberculosis expressing invasin and isogenic inv mutants. Swiss mice were infected intravenously with two strains expressing invasin (YpIII pIB1 and an isogenic virulence plasmid-cured strain, YpIII) and with two invasin mutant strains (Yp100 pIB1 and Yp100, plasmid-cured). Spleen cells were sampled on days 7, 14, 21 and 28 after infection. Immunoglobulin (Ig)-secreting spleen cells were detected by protein A plaque assay and specific antibodies were detected in sera by ELISA. The virulent strain YPIII pIB1 (wild type) did not provoke polyclonal activation of B lymphocytes in vivo. In general, fewer Ig-secreting spleen cells of all isotypes were found in the infected animals than in the control animals. Specific IgG antibodies were detected in the sera of animals infected with all strains. The peak response occurred on the 21 st day post-infection, and the Yp100 strain provoked the highest level of these antibodies. We concluded that invasin is not a polyclonal activator of murine B cells. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-12-01 2014-05-27T11:22:05Z 2014-05-27T11:22:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/385 Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 27, n. 3, p. 221-227, 2006. 1808-4532 http://hdl.handle.net/11449/69342 2-s2.0-34948873791 2-s2.0-34948873791.pdf |
url |
http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/385 http://hdl.handle.net/11449/69342 |
identifier_str_mv |
Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 27, n. 3, p. 221-227, 2006. 1808-4532 2-s2.0-34948873791 2-s2.0-34948873791.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista de Ciências Farmacêuticas Básica e Aplicada 0,131 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
221-227 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128795497988096 |