Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytes

Detalhes bibliográficos
Autor(a) principal: De Medeiros, Beatriz Maria Machado [UNESP]
Data de Publicação: 2006
Outros Autores: Cangiani, E. E. [UNESP], Higuti, L. [UNESP], Satomi, L. C. [UNESP], Souza, C. D. [UNESP], Silva, A. R C [UNESP], Maia, J. M L [UNESP], Falcão, D. P. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/385
http://hdl.handle.net/11449/69342
Resumo: It is known that the invasin molecule of Yersinia pseudotuberculosis stimulates human peripheral B cells in vitro. In this work we evaluated the in vivo role of invasin as polyclonal activator of B lymphocytes in the mouse experimental model, by comparing strains of Y. pseudotuberculosis expressing invasin and isogenic inv mutants. Swiss mice were infected intravenously with two strains expressing invasin (YpIII pIB1 and an isogenic virulence plasmid-cured strain, YpIII) and with two invasin mutant strains (Yp100 pIB1 and Yp100, plasmid-cured). Spleen cells were sampled on days 7, 14, 21 and 28 after infection. Immunoglobulin (Ig)-secreting spleen cells were detected by protein A plaque assay and specific antibodies were detected in sera by ELISA. The virulent strain YPIII pIB1 (wild type) did not provoke polyclonal activation of B lymphocytes in vivo. In general, fewer Ig-secreting spleen cells of all isotypes were found in the infected animals than in the control animals. Specific IgG antibodies were detected in the sera of animals infected with all strains. The peak response occurred on the 21 st day post-infection, and the Yp100 strain provoked the highest level of these antibodies. We concluded that invasin is not a polyclonal activator of murine B cells.
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spelling Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytesB lymphocytesInvasinPolyclonal activationSpecific antibodiesYersinia pseudotuberculosisimmunoglobulininvasinprotein Aanalytic methodanimal experimentB lymphocyte activationbacterial straincomparative studycontrolled studyenzyme linked immunosorbent assayfemalemousenonhumanspleen cellIt is known that the invasin molecule of Yersinia pseudotuberculosis stimulates human peripheral B cells in vitro. In this work we evaluated the in vivo role of invasin as polyclonal activator of B lymphocytes in the mouse experimental model, by comparing strains of Y. pseudotuberculosis expressing invasin and isogenic inv mutants. Swiss mice were infected intravenously with two strains expressing invasin (YpIII pIB1 and an isogenic virulence plasmid-cured strain, YpIII) and with two invasin mutant strains (Yp100 pIB1 and Yp100, plasmid-cured). Spleen cells were sampled on days 7, 14, 21 and 28 after infection. Immunoglobulin (Ig)-secreting spleen cells were detected by protein A plaque assay and specific antibodies were detected in sera by ELISA. The virulent strain YPIII pIB1 (wild type) did not provoke polyclonal activation of B lymphocytes in vivo. In general, fewer Ig-secreting spleen cells of all isotypes were found in the infected animals than in the control animals. Specific IgG antibodies were detected in the sera of animals infected with all strains. The peak response occurred on the 21 st day post-infection, and the Yp100 strain provoked the highest level of these antibodies. We concluded that invasin is not a polyclonal activator of murine B cells.Department of Biological Sciences School of Pharmaceutical Sciences São Paulo State University, UNESP, Araraquara, SPDepartamento de Ciências Biológicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, UNESP, Rodovia Araraquara-Jaú, km 1, CEP: 14801-902 - Araraquara - SPDepartment of Biological Sciences School of Pharmaceutical Sciences São Paulo State University, UNESP, Araraquara, SPDepartamento de Ciências Biológicas Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista, UNESP, Rodovia Araraquara-Jaú, km 1, CEP: 14801-902 - Araraquara - SPUniversidade Estadual Paulista (Unesp)De Medeiros, Beatriz Maria Machado [UNESP]Cangiani, E. E. [UNESP]Higuti, L. [UNESP]Satomi, L. C. [UNESP]Souza, C. D. [UNESP]Silva, A. R C [UNESP]Maia, J. M L [UNESP]Falcão, D. P. [UNESP]2014-05-27T11:22:05Z2014-05-27T11:22:05Z2006-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article221-227application/pdfhttp://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/385Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 27, n. 3, p. 221-227, 2006.1808-4532http://hdl.handle.net/11449/693422-s2.0-349488737912-s2.0-34948873791.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista de Ciências Farmacêuticas Básica e Aplicada0,131info:eu-repo/semantics/openAccess2024-06-24T13:07:28Zoai:repositorio.unesp.br:11449/69342Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:20:54.077940Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytes
title Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytes
spellingShingle Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytes
De Medeiros, Beatriz Maria Machado [UNESP]
B lymphocytes
Invasin
Polyclonal activation
Specific antibodies
Yersinia pseudotuberculosis
immunoglobulin
invasin
protein A
analytic method
animal experiment
B lymphocyte activation
bacterial strain
comparative study
controlled study
enzyme linked immunosorbent assay
female
mouse
nonhuman
spleen cell
title_short Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytes
title_full Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytes
title_fullStr Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytes
title_full_unstemmed Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytes
title_sort Invasin of Yersinia pseudotuberculosis is not a polyclonal activator of mouse B lymphocytes
author De Medeiros, Beatriz Maria Machado [UNESP]
author_facet De Medeiros, Beatriz Maria Machado [UNESP]
Cangiani, E. E. [UNESP]
Higuti, L. [UNESP]
Satomi, L. C. [UNESP]
Souza, C. D. [UNESP]
Silva, A. R C [UNESP]
Maia, J. M L [UNESP]
Falcão, D. P. [UNESP]
author_role author
author2 Cangiani, E. E. [UNESP]
Higuti, L. [UNESP]
Satomi, L. C. [UNESP]
Souza, C. D. [UNESP]
Silva, A. R C [UNESP]
Maia, J. M L [UNESP]
Falcão, D. P. [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv De Medeiros, Beatriz Maria Machado [UNESP]
Cangiani, E. E. [UNESP]
Higuti, L. [UNESP]
Satomi, L. C. [UNESP]
Souza, C. D. [UNESP]
Silva, A. R C [UNESP]
Maia, J. M L [UNESP]
Falcão, D. P. [UNESP]
dc.subject.por.fl_str_mv B lymphocytes
Invasin
Polyclonal activation
Specific antibodies
Yersinia pseudotuberculosis
immunoglobulin
invasin
protein A
analytic method
animal experiment
B lymphocyte activation
bacterial strain
comparative study
controlled study
enzyme linked immunosorbent assay
female
mouse
nonhuman
spleen cell
topic B lymphocytes
Invasin
Polyclonal activation
Specific antibodies
Yersinia pseudotuberculosis
immunoglobulin
invasin
protein A
analytic method
animal experiment
B lymphocyte activation
bacterial strain
comparative study
controlled study
enzyme linked immunosorbent assay
female
mouse
nonhuman
spleen cell
description It is known that the invasin molecule of Yersinia pseudotuberculosis stimulates human peripheral B cells in vitro. In this work we evaluated the in vivo role of invasin as polyclonal activator of B lymphocytes in the mouse experimental model, by comparing strains of Y. pseudotuberculosis expressing invasin and isogenic inv mutants. Swiss mice were infected intravenously with two strains expressing invasin (YpIII pIB1 and an isogenic virulence plasmid-cured strain, YpIII) and with two invasin mutant strains (Yp100 pIB1 and Yp100, plasmid-cured). Spleen cells were sampled on days 7, 14, 21 and 28 after infection. Immunoglobulin (Ig)-secreting spleen cells were detected by protein A plaque assay and specific antibodies were detected in sera by ELISA. The virulent strain YPIII pIB1 (wild type) did not provoke polyclonal activation of B lymphocytes in vivo. In general, fewer Ig-secreting spleen cells of all isotypes were found in the infected animals than in the control animals. Specific IgG antibodies were detected in the sera of animals infected with all strains. The peak response occurred on the 21 st day post-infection, and the Yp100 strain provoked the highest level of these antibodies. We concluded that invasin is not a polyclonal activator of murine B cells.
publishDate 2006
dc.date.none.fl_str_mv 2006-12-01
2014-05-27T11:22:05Z
2014-05-27T11:22:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/385
Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 27, n. 3, p. 221-227, 2006.
1808-4532
http://hdl.handle.net/11449/69342
2-s2.0-34948873791
2-s2.0-34948873791.pdf
url http://serv-bib.fcfar.unesp.br/seer/index.php/Cien_Farm/article/view/385
http://hdl.handle.net/11449/69342
identifier_str_mv Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 27, n. 3, p. 221-227, 2006.
1808-4532
2-s2.0-34948873791
2-s2.0-34948873791.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista de Ciências Farmacêuticas Básica e Aplicada
0,131
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 221-227
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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