Biological and molecular characterization of the post-invasion immature stages of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/160196 |
Resumo: | We performed molecular and biological characterizations of immature stages of Helicoverpa armigera Hubner (Lepidoptera: Noctuidae) using insects from different states in Brazil, and different host plants. Three laboratory colonies of H. armigera were established with insects from the states of Sao Paulo, Bahia, and Distrito Federal, that were collected from citrus, cotton, and corn, respectively. For each colony, microsatellites were used to assess genetic similarity among insects. The biology of the immature stages from each colony also was compared under laboratory conditions (25 +/- 1 degrees C, 70 +/- 10% RH, 14: 10 h [L: D] photoperiod). Microsatellite analysis revealed genetic variability in sampled specimens and that the grouping of individuals was independent of the geographic origin. The utility of an artificial diet was validated during the comparative biology experiment, and the ingredients of the artificial diet were analyzed for purity, particle size, and the presence and quantity of aggregates. The mean development times of the larvae and pre-pupae from the 3 locations were similar, and mean total development time (egg to adult) was not significantly different. |
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Biological and molecular characterization of the post-invasion immature stages of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae)Artificial dietlarval developmentold world bollwormstandardization of breedingmolecular characterizationWe performed molecular and biological characterizations of immature stages of Helicoverpa armigera Hubner (Lepidoptera: Noctuidae) using insects from different states in Brazil, and different host plants. Three laboratory colonies of H. armigera were established with insects from the states of Sao Paulo, Bahia, and Distrito Federal, that were collected from citrus, cotton, and corn, respectively. For each colony, microsatellites were used to assess genetic similarity among insects. The biology of the immature stages from each colony also was compared under laboratory conditions (25 +/- 1 degrees C, 70 +/- 10% RH, 14: 10 h [L: D] photoperiod). Microsatellite analysis revealed genetic variability in sampled specimens and that the grouping of individuals was independent of the geographic origin. The utility of an artificial diet was validated during the comparative biology experiment, and the ingredients of the artificial diet were analyzed for purity, particle size, and the presence and quantity of aggregates. The mean development times of the larvae and pre-pupae from the 3 locations were similar, and mean total development time (egg to adult) was not significantly different.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)EMBRAPA SEGSao Paulo State Univ, Coll Agron Sci, Dept Crop Protect, BR-18610307 Botucatu, SP, BrazilEmbrapa Cerrados, BR 020,Km 18,Caixa Postal 08223, BR-73310970 Planaltina, Distrito Federa, BrazilEmbrapa Soja, Rodovia Carlos John Str,Caixa Postal 231, BR-86001970 Londrina, Parana, BrazilUniv Brasilia, Fac UnB Planaltina, Area Univ N 1,Vila Nossa Senhora Fatima, BR-73345010 Planaltina, Distrito Federa, BrazilUniv Florida, West Florida Res & Educ Ctr, 4253 Expt Rd,Hwy 182, Jay, FL 32565 USASao Paulo State Univ, Coll Agron Sci, Dept Crop Protect, BR-18610307 Botucatu, SP, BrazilCNPq: 305649/2013-2CNPq: 308947/2014-2CNPq: 403376/2013-0CNPq: 476691/2013-3CNPq: 473041/2013-8EMBRAPA SEG: 02.13.14.006Florida Entomological SocUniversidade Estadual Paulista (Unesp)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Univ BrasiliaUniv FloridaSilva, Ivana Fernandes da [UNESP]Lopes Baldin, Edson Luiz [UNESP]Specht, AlexandreSosa-Gomez, Daniel RicardoRoque-Specht, Vania FerreiraMorando, Rafaela [UNESP]Paula-Moraes, Silvana Vieira de2018-11-26T16:00:54Z2018-11-26T16:00:54Z2018-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article25-32application/pdfFlorida Entomologist. Lutz: Florida Entomological Soc, v. 101, n. 1, p. 25-32, 2018.0015-4040http://hdl.handle.net/11449/160196WOS:000429155000005WOS000429155000005.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFlorida Entomologist0,507info:eu-repo/semantics/openAccess2024-01-28T06:44:26Zoai:repositorio.unesp.br:11449/160196Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-28T06:44:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biological and molecular characterization of the post-invasion immature stages of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) |
title |
Biological and molecular characterization of the post-invasion immature stages of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) |
spellingShingle |
Biological and molecular characterization of the post-invasion immature stages of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) Silva, Ivana Fernandes da [UNESP] Artificial diet larval development old world bollworm standardization of breeding molecular characterization |
title_short |
Biological and molecular characterization of the post-invasion immature stages of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) |
title_full |
Biological and molecular characterization of the post-invasion immature stages of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) |
title_fullStr |
Biological and molecular characterization of the post-invasion immature stages of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) |
title_full_unstemmed |
Biological and molecular characterization of the post-invasion immature stages of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) |
title_sort |
Biological and molecular characterization of the post-invasion immature stages of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae) |
author |
Silva, Ivana Fernandes da [UNESP] |
author_facet |
Silva, Ivana Fernandes da [UNESP] Lopes Baldin, Edson Luiz [UNESP] Specht, Alexandre Sosa-Gomez, Daniel Ricardo Roque-Specht, Vania Ferreira Morando, Rafaela [UNESP] Paula-Moraes, Silvana Vieira de |
author_role |
author |
author2 |
Lopes Baldin, Edson Luiz [UNESP] Specht, Alexandre Sosa-Gomez, Daniel Ricardo Roque-Specht, Vania Ferreira Morando, Rafaela [UNESP] Paula-Moraes, Silvana Vieira de |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Univ Brasilia Univ Florida |
dc.contributor.author.fl_str_mv |
Silva, Ivana Fernandes da [UNESP] Lopes Baldin, Edson Luiz [UNESP] Specht, Alexandre Sosa-Gomez, Daniel Ricardo Roque-Specht, Vania Ferreira Morando, Rafaela [UNESP] Paula-Moraes, Silvana Vieira de |
dc.subject.por.fl_str_mv |
Artificial diet larval development old world bollworm standardization of breeding molecular characterization |
topic |
Artificial diet larval development old world bollworm standardization of breeding molecular characterization |
description |
We performed molecular and biological characterizations of immature stages of Helicoverpa armigera Hubner (Lepidoptera: Noctuidae) using insects from different states in Brazil, and different host plants. Three laboratory colonies of H. armigera were established with insects from the states of Sao Paulo, Bahia, and Distrito Federal, that were collected from citrus, cotton, and corn, respectively. For each colony, microsatellites were used to assess genetic similarity among insects. The biology of the immature stages from each colony also was compared under laboratory conditions (25 +/- 1 degrees C, 70 +/- 10% RH, 14: 10 h [L: D] photoperiod). Microsatellite analysis revealed genetic variability in sampled specimens and that the grouping of individuals was independent of the geographic origin. The utility of an artificial diet was validated during the comparative biology experiment, and the ingredients of the artificial diet were analyzed for purity, particle size, and the presence and quantity of aggregates. The mean development times of the larvae and pre-pupae from the 3 locations were similar, and mean total development time (egg to adult) was not significantly different. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T16:00:54Z 2018-11-26T16:00:54Z 2018-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Florida Entomologist. Lutz: Florida Entomological Soc, v. 101, n. 1, p. 25-32, 2018. 0015-4040 http://hdl.handle.net/11449/160196 WOS:000429155000005 WOS000429155000005.pdf |
identifier_str_mv |
Florida Entomologist. Lutz: Florida Entomological Soc, v. 101, n. 1, p. 25-32, 2018. 0015-4040 WOS:000429155000005 WOS000429155000005.pdf |
url |
http://hdl.handle.net/11449/160196 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Florida Entomologist 0,507 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
25-32 application/pdf |
dc.publisher.none.fl_str_mv |
Florida Entomological Soc |
publisher.none.fl_str_mv |
Florida Entomological Soc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965746013405184 |