Improvement in ARDS experimental model installation: Low mortality rate and maintenance of hemodynamic stability
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.vascn.2012.02.003 http://hdl.handle.net/11449/231283 |
Resumo: | Introduction: Many experimental models using lung lavage have been developed for the study of acute respiratory distress syndrome (ARDS). The original technique has been modified by many authors, resulting in difficulties with reproducibility. There is insufficient detail on the lung injury models used, including hemodynamic stability during animal preparation and drawbacks encountered such as mortality. The authors studied the effects of the pulmonary recruitment and the use of fixed tidal volume (Vt) or fixed inspiratory pressure in the experimental ARDS model installation. Methods: Adult rabbits were submitted to repeated lung lavages with 30ml/kg warm saline until the ARDS definition (PaO 2/FiO 2≤100) was reached. The animals were divided into three groups, according to the technique used for mechanical ventilation: 1) fixed Vt of 10ml/kg; 2) fixed inspiratory pressure (IP) with a tidal volume of 10ml/kg prior to the first lung lavage; and 3) fixed Vt of 10ml/kg with pulmonary recruitment before the first lavage. Results: The use of alveolar recruitment maneuvers, and the use of a fixed Vt or IP between the lung lavages did not change the number of lung lavages necessary to obtain the experimental model of ARDS or the hemodynamic stability of the animals during the procedure. A trend was observed toward an increased mortality rate with the recruitment maneuver and with the use of a fixed IP. Discussion: There were no differences between the three study groups, with no disadvantage in method of lung recruitment, either fixed tidal volume or fixed inspiratory pressure, regarding the number of lung lavages necessary to obtain the ARDS animal model. Furthermore, the three different procedures resulted in good hemodynamic stability of the animals, and low mortality rate. © 2012 Elsevier Inc. |
id |
UNSP_ea25bc7c1f5d478a504bb93db993c08d |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/231283 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Improvement in ARDS experimental model installation: Low mortality rate and maintenance of hemodynamic stabilityAdult respiratory distress syndromeAnimal modelsBronchoalveolar lavageTidal volumeIntroduction: Many experimental models using lung lavage have been developed for the study of acute respiratory distress syndrome (ARDS). The original technique has been modified by many authors, resulting in difficulties with reproducibility. There is insufficient detail on the lung injury models used, including hemodynamic stability during animal preparation and drawbacks encountered such as mortality. The authors studied the effects of the pulmonary recruitment and the use of fixed tidal volume (Vt) or fixed inspiratory pressure in the experimental ARDS model installation. Methods: Adult rabbits were submitted to repeated lung lavages with 30ml/kg warm saline until the ARDS definition (PaO 2/FiO 2≤100) was reached. The animals were divided into three groups, according to the technique used for mechanical ventilation: 1) fixed Vt of 10ml/kg; 2) fixed inspiratory pressure (IP) with a tidal volume of 10ml/kg prior to the first lung lavage; and 3) fixed Vt of 10ml/kg with pulmonary recruitment before the first lavage. Results: The use of alveolar recruitment maneuvers, and the use of a fixed Vt or IP between the lung lavages did not change the number of lung lavages necessary to obtain the experimental model of ARDS or the hemodynamic stability of the animals during the procedure. A trend was observed toward an increased mortality rate with the recruitment maneuver and with the use of a fixed IP. Discussion: There were no differences between the three study groups, with no disadvantage in method of lung recruitment, either fixed tidal volume or fixed inspiratory pressure, regarding the number of lung lavages necessary to obtain the ARDS animal model. Furthermore, the three different procedures resulted in good hemodynamic stability of the animals, and low mortality rate. © 2012 Elsevier Inc.Albert Einstein Jewish HospitalIntegrated College of Bauru State University Julio de Mesquita FilhoUniversity of São Paulo Medical School Department of PediatricsIntegrated College of Bauru State University Julio de Mesquita FilhoAlbert Einstein Jewish HospitalUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Haddad, Luciana BrancoManzano, Roberta Munhoz [UNESP]Rossi, Felipe de SouzaFreddi, Norberto AntonioPrado, CristianeRebello, Celso Moura2022-04-29T08:44:29Z2022-04-29T08:44:29Z2012-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article102-106http://dx.doi.org/10.1016/j.vascn.2012.02.003Journal of Pharmacological and Toxicological Methods, v. 65, n. 3, p. 102-106, 2012.1056-87191873-488Xhttp://hdl.handle.net/11449/23128310.1016/j.vascn.2012.02.0032-s2.0-84861483246Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Pharmacological and Toxicological Methodsinfo:eu-repo/semantics/openAccess2022-04-29T08:44:29Zoai:repositorio.unesp.br:11449/231283Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:44:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Improvement in ARDS experimental model installation: Low mortality rate and maintenance of hemodynamic stability |
title |
Improvement in ARDS experimental model installation: Low mortality rate and maintenance of hemodynamic stability |
spellingShingle |
Improvement in ARDS experimental model installation: Low mortality rate and maintenance of hemodynamic stability Haddad, Luciana Branco Adult respiratory distress syndrome Animal models Bronchoalveolar lavage Tidal volume |
title_short |
Improvement in ARDS experimental model installation: Low mortality rate and maintenance of hemodynamic stability |
title_full |
Improvement in ARDS experimental model installation: Low mortality rate and maintenance of hemodynamic stability |
title_fullStr |
Improvement in ARDS experimental model installation: Low mortality rate and maintenance of hemodynamic stability |
title_full_unstemmed |
Improvement in ARDS experimental model installation: Low mortality rate and maintenance of hemodynamic stability |
title_sort |
Improvement in ARDS experimental model installation: Low mortality rate and maintenance of hemodynamic stability |
author |
Haddad, Luciana Branco |
author_facet |
Haddad, Luciana Branco Manzano, Roberta Munhoz [UNESP] Rossi, Felipe de Souza Freddi, Norberto Antonio Prado, Cristiane Rebello, Celso Moura |
author_role |
author |
author2 |
Manzano, Roberta Munhoz [UNESP] Rossi, Felipe de Souza Freddi, Norberto Antonio Prado, Cristiane Rebello, Celso Moura |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Albert Einstein Jewish Hospital Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Haddad, Luciana Branco Manzano, Roberta Munhoz [UNESP] Rossi, Felipe de Souza Freddi, Norberto Antonio Prado, Cristiane Rebello, Celso Moura |
dc.subject.por.fl_str_mv |
Adult respiratory distress syndrome Animal models Bronchoalveolar lavage Tidal volume |
topic |
Adult respiratory distress syndrome Animal models Bronchoalveolar lavage Tidal volume |
description |
Introduction: Many experimental models using lung lavage have been developed for the study of acute respiratory distress syndrome (ARDS). The original technique has been modified by many authors, resulting in difficulties with reproducibility. There is insufficient detail on the lung injury models used, including hemodynamic stability during animal preparation and drawbacks encountered such as mortality. The authors studied the effects of the pulmonary recruitment and the use of fixed tidal volume (Vt) or fixed inspiratory pressure in the experimental ARDS model installation. Methods: Adult rabbits were submitted to repeated lung lavages with 30ml/kg warm saline until the ARDS definition (PaO 2/FiO 2≤100) was reached. The animals were divided into three groups, according to the technique used for mechanical ventilation: 1) fixed Vt of 10ml/kg; 2) fixed inspiratory pressure (IP) with a tidal volume of 10ml/kg prior to the first lung lavage; and 3) fixed Vt of 10ml/kg with pulmonary recruitment before the first lavage. Results: The use of alveolar recruitment maneuvers, and the use of a fixed Vt or IP between the lung lavages did not change the number of lung lavages necessary to obtain the experimental model of ARDS or the hemodynamic stability of the animals during the procedure. A trend was observed toward an increased mortality rate with the recruitment maneuver and with the use of a fixed IP. Discussion: There were no differences between the three study groups, with no disadvantage in method of lung recruitment, either fixed tidal volume or fixed inspiratory pressure, regarding the number of lung lavages necessary to obtain the ARDS animal model. Furthermore, the three different procedures resulted in good hemodynamic stability of the animals, and low mortality rate. © 2012 Elsevier Inc. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-05-01 2022-04-29T08:44:29Z 2022-04-29T08:44:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.vascn.2012.02.003 Journal of Pharmacological and Toxicological Methods, v. 65, n. 3, p. 102-106, 2012. 1056-8719 1873-488X http://hdl.handle.net/11449/231283 10.1016/j.vascn.2012.02.003 2-s2.0-84861483246 |
url |
http://dx.doi.org/10.1016/j.vascn.2012.02.003 http://hdl.handle.net/11449/231283 |
identifier_str_mv |
Journal of Pharmacological and Toxicological Methods, v. 65, n. 3, p. 102-106, 2012. 1056-8719 1873-488X 10.1016/j.vascn.2012.02.003 2-s2.0-84861483246 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Pharmacological and Toxicological Methods |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
102-106 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797789809344774144 |