Selective anticancer effects of Serjania marginata Casar. extract in gastric cells are mediated by antioxidant response

Detalhes bibliográficos
Autor(a) principal: Serpeloni, Juliana Mara
Data de Publicação: 2021
Outros Autores: Specian, Ana Flavia Leal, Ribeiro, Diego Luis, Tuttis, Katiuska, Heredia-Vieira, Silvia Cristina, Vilegas, Wagner [UNESP], Martínez-López, Wilner, Varanda, Eliana Aparecida [UNESP], de Syllos Cólus, Ilce Mara
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1002/tox.23151
http://hdl.handle.net/11449/207640
Resumo: Gastric cancer is the fifth most common malignancy worldwide. Serjania marginata Casar. (SM) displays anti-inflammatory properties and has been used to treat gastrointestinal disorders. In the current study, we examined whether the hydroethanolic extract of SM leaves exerted cytotoxic, mutagenic, and protective effects in non-tumor gastric epithelium cells (MNP01) and gastric adenocarcinoma cells (ACP02) in vitro and analyzed whether its action was selective. Initially, cell viability (MTT assay), cell cycle kinetics (flow cytometry), and cell proliferation (total protein content) were analyzed. In addition, genomic instability (cytokinesis-block micronucleus cytome assay), anti/pro-oxidant status (CM-H2DCFDA probe), and transcriptional expression (RT-qPCR) of genes related to cell cycle, cell death, and antioxidant defense were also evaluated. The SM extract was cytotoxic toward MNP01 and ACP02 cells at concentrations greater than 300 and 100 μg·ml−1, respectively, and decreased protein content only toward ACP02 cells at 200 μg ml−1. In ACP02 cells, the SM extract at 100 μg·ml−1 associated with doxorubicin (DXR; 0.2 μg ml−1) clearly promoted cell cycle arrest at the G2/M phase. The extract alone was not mutagenic to either cell type and reversed DXR-induced DNA damage and H2O2-induced oxidative stress in MNP01 cells. The gene expression experiments showed that SM hydroethanolic extract exerts an antioxidant response via NFE2L2 activation in non-tumor gastric cells, and cell cycle arrest (G2/M) in ACP02 gastric cancer cells via the TP53 pathway. The selective action of SM indicates that it is a promising therapeutic agent to treat gastric diseases and merits further studies.
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spelling Selective anticancer effects of Serjania marginata Casar. extract in gastric cells are mediated by antioxidant responseantimutagenicantioxidantcipó-timbócytotoxicitySapindaceaeGastric cancer is the fifth most common malignancy worldwide. Serjania marginata Casar. (SM) displays anti-inflammatory properties and has been used to treat gastrointestinal disorders. In the current study, we examined whether the hydroethanolic extract of SM leaves exerted cytotoxic, mutagenic, and protective effects in non-tumor gastric epithelium cells (MNP01) and gastric adenocarcinoma cells (ACP02) in vitro and analyzed whether its action was selective. Initially, cell viability (MTT assay), cell cycle kinetics (flow cytometry), and cell proliferation (total protein content) were analyzed. In addition, genomic instability (cytokinesis-block micronucleus cytome assay), anti/pro-oxidant status (CM-H2DCFDA probe), and transcriptional expression (RT-qPCR) of genes related to cell cycle, cell death, and antioxidant defense were also evaluated. The SM extract was cytotoxic toward MNP01 and ACP02 cells at concentrations greater than 300 and 100 μg·ml−1, respectively, and decreased protein content only toward ACP02 cells at 200 μg ml−1. In ACP02 cells, the SM extract at 100 μg·ml−1 associated with doxorubicin (DXR; 0.2 μg ml−1) clearly promoted cell cycle arrest at the G2/M phase. The extract alone was not mutagenic to either cell type and reversed DXR-induced DNA damage and H2O2-induced oxidative stress in MNP01 cells. The gene expression experiments showed that SM hydroethanolic extract exerts an antioxidant response via NFE2L2 activation in non-tumor gastric cells, and cell cycle arrest (G2/M) in ACP02 gastric cancer cells via the TP53 pathway. The selective action of SM indicates that it is a promising therapeutic agent to treat gastric diseases and merits further studies.Department of General Biology Center of Biological Sciences State University of Londrina (UEL)Natural Products Laboratory Anhanguera-Uniderp UniversityExperimental Campus of São Vicente São Paulo State University (UNESP)Epigenetics and Genomic Instability Laboratory Instituto de Investigaciones Biológicas Clemente EstableDepartment of Biological Sciences School of Pharmaceutical Sciences São Paulo State University (UNESP)Experimental Campus of São Vicente São Paulo State University (UNESP)Department of Biological Sciences School of Pharmaceutical Sciences São Paulo State University (UNESP)Universidade Estadual de Londrina (UEL)Anhanguera-Uniderp UniversityUniversidade Estadual Paulista (Unesp)Instituto de Investigaciones Biológicas Clemente EstableSerpeloni, Juliana MaraSpecian, Ana Flavia LealRibeiro, Diego LuisTuttis, KatiuskaHeredia-Vieira, Silvia CristinaVilegas, Wagner [UNESP]Martínez-López, WilnerVaranda, Eliana Aparecida [UNESP]de Syllos Cólus, Ilce Mara2021-06-25T10:58:32Z2021-06-25T10:58:32Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/tox.23151Environmental Toxicology.1522-72781520-4081http://hdl.handle.net/11449/20764010.1002/tox.231512-s2.0-85104644603Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Toxicologyinfo:eu-repo/semantics/openAccess2024-06-24T13:08:13Zoai:repositorio.unesp.br:11449/207640Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:05:03.182379Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Selective anticancer effects of Serjania marginata Casar. extract in gastric cells are mediated by antioxidant response
title Selective anticancer effects of Serjania marginata Casar. extract in gastric cells are mediated by antioxidant response
spellingShingle Selective anticancer effects of Serjania marginata Casar. extract in gastric cells are mediated by antioxidant response
Serpeloni, Juliana Mara
antimutagenic
antioxidant
cipó-timbó
cytotoxicity
Sapindaceae
title_short Selective anticancer effects of Serjania marginata Casar. extract in gastric cells are mediated by antioxidant response
title_full Selective anticancer effects of Serjania marginata Casar. extract in gastric cells are mediated by antioxidant response
title_fullStr Selective anticancer effects of Serjania marginata Casar. extract in gastric cells are mediated by antioxidant response
title_full_unstemmed Selective anticancer effects of Serjania marginata Casar. extract in gastric cells are mediated by antioxidant response
title_sort Selective anticancer effects of Serjania marginata Casar. extract in gastric cells are mediated by antioxidant response
author Serpeloni, Juliana Mara
author_facet Serpeloni, Juliana Mara
Specian, Ana Flavia Leal
Ribeiro, Diego Luis
Tuttis, Katiuska
Heredia-Vieira, Silvia Cristina
Vilegas, Wagner [UNESP]
Martínez-López, Wilner
Varanda, Eliana Aparecida [UNESP]
de Syllos Cólus, Ilce Mara
author_role author
author2 Specian, Ana Flavia Leal
Ribeiro, Diego Luis
Tuttis, Katiuska
Heredia-Vieira, Silvia Cristina
Vilegas, Wagner [UNESP]
Martínez-López, Wilner
Varanda, Eliana Aparecida [UNESP]
de Syllos Cólus, Ilce Mara
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Londrina (UEL)
Anhanguera-Uniderp University
Universidade Estadual Paulista (Unesp)
Instituto de Investigaciones Biológicas Clemente Estable
dc.contributor.author.fl_str_mv Serpeloni, Juliana Mara
Specian, Ana Flavia Leal
Ribeiro, Diego Luis
Tuttis, Katiuska
Heredia-Vieira, Silvia Cristina
Vilegas, Wagner [UNESP]
Martínez-López, Wilner
Varanda, Eliana Aparecida [UNESP]
de Syllos Cólus, Ilce Mara
dc.subject.por.fl_str_mv antimutagenic
antioxidant
cipó-timbó
cytotoxicity
Sapindaceae
topic antimutagenic
antioxidant
cipó-timbó
cytotoxicity
Sapindaceae
description Gastric cancer is the fifth most common malignancy worldwide. Serjania marginata Casar. (SM) displays anti-inflammatory properties and has been used to treat gastrointestinal disorders. In the current study, we examined whether the hydroethanolic extract of SM leaves exerted cytotoxic, mutagenic, and protective effects in non-tumor gastric epithelium cells (MNP01) and gastric adenocarcinoma cells (ACP02) in vitro and analyzed whether its action was selective. Initially, cell viability (MTT assay), cell cycle kinetics (flow cytometry), and cell proliferation (total protein content) were analyzed. In addition, genomic instability (cytokinesis-block micronucleus cytome assay), anti/pro-oxidant status (CM-H2DCFDA probe), and transcriptional expression (RT-qPCR) of genes related to cell cycle, cell death, and antioxidant defense were also evaluated. The SM extract was cytotoxic toward MNP01 and ACP02 cells at concentrations greater than 300 and 100 μg·ml−1, respectively, and decreased protein content only toward ACP02 cells at 200 μg ml−1. In ACP02 cells, the SM extract at 100 μg·ml−1 associated with doxorubicin (DXR; 0.2 μg ml−1) clearly promoted cell cycle arrest at the G2/M phase. The extract alone was not mutagenic to either cell type and reversed DXR-induced DNA damage and H2O2-induced oxidative stress in MNP01 cells. The gene expression experiments showed that SM hydroethanolic extract exerts an antioxidant response via NFE2L2 activation in non-tumor gastric cells, and cell cycle arrest (G2/M) in ACP02 gastric cancer cells via the TP53 pathway. The selective action of SM indicates that it is a promising therapeutic agent to treat gastric diseases and merits further studies.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T10:58:32Z
2021-06-25T10:58:32Z
2021-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/tox.23151
Environmental Toxicology.
1522-7278
1520-4081
http://hdl.handle.net/11449/207640
10.1002/tox.23151
2-s2.0-85104644603
url http://dx.doi.org/10.1002/tox.23151
http://hdl.handle.net/11449/207640
identifier_str_mv Environmental Toxicology.
1522-7278
1520-4081
10.1002/tox.23151
2-s2.0-85104644603
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Environmental Toxicology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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