Validation of ACB in vitro and in vivo as a biomagnetic method for measuring stomach contraction
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/j.1365-2982.2010.01582.x http://hdl.handle.net/11449/17687 |
Resumo: | Background The aim of this study was to validate a biomagnetic method (alternate current biosusceptometry, ACB) for monitoring gastric wall contractions in rats. Methods In vitro data were obtained to establish the relationship between ACB and the strain-gauge (SG) signal amplitude. In vivo experiments were performed in pentobarbital-anesthetized rats with SG and magnetic markers previously implanted under the gastric serosa or after ingestion of magnetic material. Gastric motility was quantified from the tracing amplitudes and frequency profiles obtained by Fast Fourier Transform. Key Results The correlation between in vitro signal amplitudes was strong (R = 0.989). The temporal cross-correlation coefficient between the ACB and SG signal amplitude was higher (P < 0.0001) in the postprandial (88.3 +/- 9.1 V) than in the fasting state (31.0 +/- 16.9 V). Irregular signal profiles, low contraction amplitudes, and smaller signal-to-noise ratios explained the poor correlation between techniques for fasting-state recordings. When a magnetic material was ingested, there was also strong correlation in the frequency and signal amplitude and a small phase-difference between the techniques. The contraction frequencies using ACB were 0.068 +/- 0.007 Hz (postprandial) and 0.058 +/- 0.007 Hz (fasting) (P < 0.002) and those using SG were 0.066 +/- 0.006 Hz (postprandial) and 0.059 +/- 0.008 Hz (fasting) (P < 0.005). Conclusions & Inferences In summary, ACB is reliable for monitoring gastric wall contractions using both implanted and ingested magnetic materials, and may serve as an accurate and sensitive technique for gastrointestinal motility studies. |
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Validation of ACB in vitro and in vivo as a biomagnetic method for measuring stomach contractionDigestive physiologyFoodGastrointestinal motilityInvestigative techniquesRatsTransducersBackground The aim of this study was to validate a biomagnetic method (alternate current biosusceptometry, ACB) for monitoring gastric wall contractions in rats. Methods In vitro data were obtained to establish the relationship between ACB and the strain-gauge (SG) signal amplitude. In vivo experiments were performed in pentobarbital-anesthetized rats with SG and magnetic markers previously implanted under the gastric serosa or after ingestion of magnetic material. Gastric motility was quantified from the tracing amplitudes and frequency profiles obtained by Fast Fourier Transform. Key Results The correlation between in vitro signal amplitudes was strong (R = 0.989). The temporal cross-correlation coefficient between the ACB and SG signal amplitude was higher (P < 0.0001) in the postprandial (88.3 +/- 9.1 V) than in the fasting state (31.0 +/- 16.9 V). Irregular signal profiles, low contraction amplitudes, and smaller signal-to-noise ratios explained the poor correlation between techniques for fasting-state recordings. When a magnetic material was ingested, there was also strong correlation in the frequency and signal amplitude and a small phase-difference between the techniques. The contraction frequencies using ACB were 0.068 +/- 0.007 Hz (postprandial) and 0.058 +/- 0.007 Hz (fasting) (P < 0.002) and those using SG were 0.066 +/- 0.006 Hz (postprandial) and 0.059 +/- 0.008 Hz (fasting) (P < 0.005). Conclusions & Inferences In summary, ACB is reliable for monitoring gastric wall contractions using both implanted and ingested magnetic materials, and may serve as an accurate and sensitive technique for gastrointestinal motility studies.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)UNESP Univ Estadual Paulista, Dept Fis & Biofis, Inst Biociencias, BR-18618000 São Paulo, BrazilUFMT Univ Mato Grosso, Inst Ciencias Biol & Saude, Barra do Garcas, Mato Grosso, BrazilUniv Estadual Paulista UNESP, Fac Med, Dept Cirurgia & Ortopedia, BR-18618000 São Paulo, BrazilUniv São Paulo, Fac Med, Dept Clin Med, São Paulo, BrazilUNCISAL Alagoas State Univ Hlth Sci, Maceio, Alagoas, BrazilUNESP Univ Estadual Paulista, Dept Fis & Biofis, Inst Biociencias, BR-18618000 São Paulo, BrazilUniv Estadual Paulista UNESP, Fac Med, Dept Cirurgia & Ortopedia, BR-18618000 São Paulo, BrazilWiley-Blackwell Publishing, IncUniversidade Estadual Paulista (Unesp)Universidade Federal de Mato Grosso (UFMT)Universidade de São Paulo (USP)Universidade Estadual de Ciências da Saúde de Alagoas (UNCISAL)Americo, Madileine Francely [UNESP]Marques, Rozemeire Garcia [UNESP]Zandona, E. A. [UNESP]Andreis, Uilian de [UNESP]Stelzer, Marcelo [UNESP]Cora, Luciana AparecidaOliveira, Ricardo Brandt deMiranda, José Ricardo de Arruda [UNESP]2014-05-20T13:49:36Z2014-05-20T13:49:36Z2010-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1340-1374http://dx.doi.org/10.1111/j.1365-2982.2010.01582.xNeurogastroenterology and Motility. Malden: Wiley-blackwell Publishing, Inc, v. 22, n. 12, p. 1340-1374, 2010.1350-1925http://hdl.handle.net/11449/1768710.1111/j.1365-2982.2010.01582.xWOS:00028398850001550837404159116730000-0002-5928-5608Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengNeurogastroenterology and Motility3.8422,095info:eu-repo/semantics/openAccess2024-08-14T14:18:40Zoai:repositorio.unesp.br:11449/17687Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T14:18:40Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Validation of ACB in vitro and in vivo as a biomagnetic method for measuring stomach contraction |
title |
Validation of ACB in vitro and in vivo as a biomagnetic method for measuring stomach contraction |
spellingShingle |
Validation of ACB in vitro and in vivo as a biomagnetic method for measuring stomach contraction Americo, Madileine Francely [UNESP] Digestive physiology Food Gastrointestinal motility Investigative techniques Rats Transducers |
title_short |
Validation of ACB in vitro and in vivo as a biomagnetic method for measuring stomach contraction |
title_full |
Validation of ACB in vitro and in vivo as a biomagnetic method for measuring stomach contraction |
title_fullStr |
Validation of ACB in vitro and in vivo as a biomagnetic method for measuring stomach contraction |
title_full_unstemmed |
Validation of ACB in vitro and in vivo as a biomagnetic method for measuring stomach contraction |
title_sort |
Validation of ACB in vitro and in vivo as a biomagnetic method for measuring stomach contraction |
author |
Americo, Madileine Francely [UNESP] |
author_facet |
Americo, Madileine Francely [UNESP] Marques, Rozemeire Garcia [UNESP] Zandona, E. A. [UNESP] Andreis, Uilian de [UNESP] Stelzer, Marcelo [UNESP] Cora, Luciana Aparecida Oliveira, Ricardo Brandt de Miranda, José Ricardo de Arruda [UNESP] |
author_role |
author |
author2 |
Marques, Rozemeire Garcia [UNESP] Zandona, E. A. [UNESP] Andreis, Uilian de [UNESP] Stelzer, Marcelo [UNESP] Cora, Luciana Aparecida Oliveira, Ricardo Brandt de Miranda, José Ricardo de Arruda [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Mato Grosso (UFMT) Universidade de São Paulo (USP) Universidade Estadual de Ciências da Saúde de Alagoas (UNCISAL) |
dc.contributor.author.fl_str_mv |
Americo, Madileine Francely [UNESP] Marques, Rozemeire Garcia [UNESP] Zandona, E. A. [UNESP] Andreis, Uilian de [UNESP] Stelzer, Marcelo [UNESP] Cora, Luciana Aparecida Oliveira, Ricardo Brandt de Miranda, José Ricardo de Arruda [UNESP] |
dc.subject.por.fl_str_mv |
Digestive physiology Food Gastrointestinal motility Investigative techniques Rats Transducers |
topic |
Digestive physiology Food Gastrointestinal motility Investigative techniques Rats Transducers |
description |
Background The aim of this study was to validate a biomagnetic method (alternate current biosusceptometry, ACB) for monitoring gastric wall contractions in rats. Methods In vitro data were obtained to establish the relationship between ACB and the strain-gauge (SG) signal amplitude. In vivo experiments were performed in pentobarbital-anesthetized rats with SG and magnetic markers previously implanted under the gastric serosa or after ingestion of magnetic material. Gastric motility was quantified from the tracing amplitudes and frequency profiles obtained by Fast Fourier Transform. Key Results The correlation between in vitro signal amplitudes was strong (R = 0.989). The temporal cross-correlation coefficient between the ACB and SG signal amplitude was higher (P < 0.0001) in the postprandial (88.3 +/- 9.1 V) than in the fasting state (31.0 +/- 16.9 V). Irregular signal profiles, low contraction amplitudes, and smaller signal-to-noise ratios explained the poor correlation between techniques for fasting-state recordings. When a magnetic material was ingested, there was also strong correlation in the frequency and signal amplitude and a small phase-difference between the techniques. The contraction frequencies using ACB were 0.068 +/- 0.007 Hz (postprandial) and 0.058 +/- 0.007 Hz (fasting) (P < 0.002) and those using SG were 0.066 +/- 0.006 Hz (postprandial) and 0.059 +/- 0.008 Hz (fasting) (P < 0.005). Conclusions & Inferences In summary, ACB is reliable for monitoring gastric wall contractions using both implanted and ingested magnetic materials, and may serve as an accurate and sensitive technique for gastrointestinal motility studies. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-12-01 2014-05-20T13:49:36Z 2014-05-20T13:49:36Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/j.1365-2982.2010.01582.x Neurogastroenterology and Motility. Malden: Wiley-blackwell Publishing, Inc, v. 22, n. 12, p. 1340-1374, 2010. 1350-1925 http://hdl.handle.net/11449/17687 10.1111/j.1365-2982.2010.01582.x WOS:000283988500015 5083740415911673 0000-0002-5928-5608 |
url |
http://dx.doi.org/10.1111/j.1365-2982.2010.01582.x http://hdl.handle.net/11449/17687 |
identifier_str_mv |
Neurogastroenterology and Motility. Malden: Wiley-blackwell Publishing, Inc, v. 22, n. 12, p. 1340-1374, 2010. 1350-1925 10.1111/j.1365-2982.2010.01582.x WOS:000283988500015 5083740415911673 0000-0002-5928-5608 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Neurogastroenterology and Motility 3.842 2,095 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1340-1374 |
dc.publisher.none.fl_str_mv |
Wiley-Blackwell Publishing, Inc |
publisher.none.fl_str_mv |
Wiley-Blackwell Publishing, Inc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128120201412608 |