Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2015/750752 http://hdl.handle.net/11449/158457 |
Resumo: | To study cocaine's toxic effects in vitro, we have used primary mesencephalic and striatal cultures from rat embryonic brain. Treatment with cocaine causes a dramatic increase in DNA fragmentation in both primary cultures. The toxicity induced by cocaine was paralleled with a concomitant decrease in the microtubule associated protein 2 (MAP2) and/or neuronal nucleus protein (NeuN) staining. We also observed in both cultures that the cell death caused by cocaine was induced by an apoptotic mechanism, confirmed by TUNEL assay. Therefore, the present paper shows that cocaine causes apoptotic cell death and inhibition of the neurite prolongation in striatal and mesencephalic cell culture. These data suggest that if similar neuronal damage could be produced in the developing human brain, it could account for the qualitative or quantitative defects in neuronal pathways that cause a major handicap in brain function following prenatal exposure to cocaine. |
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Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary CulturesTo study cocaine's toxic effects in vitro, we have used primary mesencephalic and striatal cultures from rat embryonic brain. Treatment with cocaine causes a dramatic increase in DNA fragmentation in both primary cultures. The toxicity induced by cocaine was paralleled with a concomitant decrease in the microtubule associated protein 2 (MAP2) and/or neuronal nucleus protein (NeuN) staining. We also observed in both cultures that the cell death caused by cocaine was induced by an apoptotic mechanism, confirmed by TUNEL assay. Therefore, the present paper shows that cocaine causes apoptotic cell death and inhibition of the neurite prolongation in striatal and mesencephalic cell culture. These data suggest that if similar neuronal damage could be produced in the developing human brain, it could account for the qualitative or quantitative defects in neuronal pathways that cause a major handicap in brain function following prenatal exposure to cocaine.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)University of Sao PauloCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Center for Neuroscience, Aging, and Stem Cell Research, Burnham Institute for Medical Research, La Jolla, CA, USAUniv Sao Paulo, Inst Biomed Sci, Dept Pharmacol, BR-05508900 Sao Paulo, SP, BrazilLIBBS Co, Sao Paulo, SP, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut, Lab Neuropsicofarmacol, Araraquara, SP, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut, Lab Neuropsicofarmacol, Araraquara, SP, BrazilFAPESP: 2006/50105-0FAPESP: 2012/50165-3University of Sao Paulo: 2011.1.9333.1.3Hindawi Publishing CorporationUniversidade de São Paulo (USP)LIBBS CoUniversidade Estadual Paulista (Unesp)Lepsch, Lucilia B.Planeta, Cleopatra S. [UNESP]Scavone, Critoforo2018-11-26T15:27:45Z2018-11-26T15:27:45Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://dx.doi.org/10.1155/2015/750752Biomed Research International. New York: Hindawi Publishing Corporation, 7 p., 2015.2314-6133http://hdl.handle.net/11449/15845710.1155/2015/750752WOS:000359237100001WOS000359237100001.pdf25147625452809420000-0002-1378-6327Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomed Research International0,935info:eu-repo/semantics/openAccess2024-01-28T06:44:24Zoai:repositorio.unesp.br:11449/158457Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:07:21.413435Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures |
title |
Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures |
spellingShingle |
Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures Lepsch, Lucilia B. |
title_short |
Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures |
title_full |
Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures |
title_fullStr |
Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures |
title_full_unstemmed |
Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures |
title_sort |
Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures |
author |
Lepsch, Lucilia B. |
author_facet |
Lepsch, Lucilia B. Planeta, Cleopatra S. [UNESP] Scavone, Critoforo |
author_role |
author |
author2 |
Planeta, Cleopatra S. [UNESP] Scavone, Critoforo |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) LIBBS Co Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Lepsch, Lucilia B. Planeta, Cleopatra S. [UNESP] Scavone, Critoforo |
description |
To study cocaine's toxic effects in vitro, we have used primary mesencephalic and striatal cultures from rat embryonic brain. Treatment with cocaine causes a dramatic increase in DNA fragmentation in both primary cultures. The toxicity induced by cocaine was paralleled with a concomitant decrease in the microtubule associated protein 2 (MAP2) and/or neuronal nucleus protein (NeuN) staining. We also observed in both cultures that the cell death caused by cocaine was induced by an apoptotic mechanism, confirmed by TUNEL assay. Therefore, the present paper shows that cocaine causes apoptotic cell death and inhibition of the neurite prolongation in striatal and mesencephalic cell culture. These data suggest that if similar neuronal damage could be produced in the developing human brain, it could account for the qualitative or quantitative defects in neuronal pathways that cause a major handicap in brain function following prenatal exposure to cocaine. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-01 2018-11-26T15:27:45Z 2018-11-26T15:27:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2015/750752 Biomed Research International. New York: Hindawi Publishing Corporation, 7 p., 2015. 2314-6133 http://hdl.handle.net/11449/158457 10.1155/2015/750752 WOS:000359237100001 WOS000359237100001.pdf 2514762545280942 0000-0002-1378-6327 |
url |
http://dx.doi.org/10.1155/2015/750752 http://hdl.handle.net/11449/158457 |
identifier_str_mv |
Biomed Research International. New York: Hindawi Publishing Corporation, 7 p., 2015. 2314-6133 10.1155/2015/750752 WOS:000359237100001 WOS000359237100001.pdf 2514762545280942 0000-0002-1378-6327 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biomed Research International 0,935 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129586195595264 |