Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures

Detalhes bibliográficos
Autor(a) principal: Lepsch, Lucilia B.
Data de Publicação: 2015
Outros Autores: Planeta, Cleopatra S. [UNESP], Scavone, Critoforo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1155/2015/750752
http://hdl.handle.net/11449/158457
Resumo: To study cocaine's toxic effects in vitro, we have used primary mesencephalic and striatal cultures from rat embryonic brain. Treatment with cocaine causes a dramatic increase in DNA fragmentation in both primary cultures. The toxicity induced by cocaine was paralleled with a concomitant decrease in the microtubule associated protein 2 (MAP2) and/or neuronal nucleus protein (NeuN) staining. We also observed in both cultures that the cell death caused by cocaine was induced by an apoptotic mechanism, confirmed by TUNEL assay. Therefore, the present paper shows that cocaine causes apoptotic cell death and inhibition of the neurite prolongation in striatal and mesencephalic cell culture. These data suggest that if similar neuronal damage could be produced in the developing human brain, it could account for the qualitative or quantitative defects in neuronal pathways that cause a major handicap in brain function following prenatal exposure to cocaine.
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spelling Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary CulturesTo study cocaine's toxic effects in vitro, we have used primary mesencephalic and striatal cultures from rat embryonic brain. Treatment with cocaine causes a dramatic increase in DNA fragmentation in both primary cultures. The toxicity induced by cocaine was paralleled with a concomitant decrease in the microtubule associated protein 2 (MAP2) and/or neuronal nucleus protein (NeuN) staining. We also observed in both cultures that the cell death caused by cocaine was induced by an apoptotic mechanism, confirmed by TUNEL assay. Therefore, the present paper shows that cocaine causes apoptotic cell death and inhibition of the neurite prolongation in striatal and mesencephalic cell culture. These data suggest that if similar neuronal damage could be produced in the developing human brain, it could account for the qualitative or quantitative defects in neuronal pathways that cause a major handicap in brain function following prenatal exposure to cocaine.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)University of Sao PauloCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Center for Neuroscience, Aging, and Stem Cell Research, Burnham Institute for Medical Research, La Jolla, CA, USAUniv Sao Paulo, Inst Biomed Sci, Dept Pharmacol, BR-05508900 Sao Paulo, SP, BrazilLIBBS Co, Sao Paulo, SP, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut, Lab Neuropsicofarmacol, Araraquara, SP, BrazilUniv Estadual Paulista, Fac Ciencias Farmaceut, Lab Neuropsicofarmacol, Araraquara, SP, BrazilFAPESP: 2006/50105-0FAPESP: 2012/50165-3University of Sao Paulo: 2011.1.9333.1.3Hindawi Publishing CorporationUniversidade de São Paulo (USP)LIBBS CoUniversidade Estadual Paulista (Unesp)Lepsch, Lucilia B.Planeta, Cleopatra S. [UNESP]Scavone, Critoforo2018-11-26T15:27:45Z2018-11-26T15:27:45Z2015-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://dx.doi.org/10.1155/2015/750752Biomed Research International. New York: Hindawi Publishing Corporation, 7 p., 2015.2314-6133http://hdl.handle.net/11449/15845710.1155/2015/750752WOS:000359237100001WOS000359237100001.pdf25147625452809420000-0002-1378-6327Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiomed Research International0,935info:eu-repo/semantics/openAccess2024-01-28T06:44:24Zoai:repositorio.unesp.br:11449/158457Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-06T00:07:21.413435Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures
title Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures
spellingShingle Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures
Lepsch, Lucilia B.
title_short Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures
title_full Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures
title_fullStr Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures
title_full_unstemmed Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures
title_sort Cocaine Causes Apoptotic Death in Rat Mesencephalon and Striatum Primary Cultures
author Lepsch, Lucilia B.
author_facet Lepsch, Lucilia B.
Planeta, Cleopatra S. [UNESP]
Scavone, Critoforo
author_role author
author2 Planeta, Cleopatra S. [UNESP]
Scavone, Critoforo
author2_role author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
LIBBS Co
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Lepsch, Lucilia B.
Planeta, Cleopatra S. [UNESP]
Scavone, Critoforo
description To study cocaine's toxic effects in vitro, we have used primary mesencephalic and striatal cultures from rat embryonic brain. Treatment with cocaine causes a dramatic increase in DNA fragmentation in both primary cultures. The toxicity induced by cocaine was paralleled with a concomitant decrease in the microtubule associated protein 2 (MAP2) and/or neuronal nucleus protein (NeuN) staining. We also observed in both cultures that the cell death caused by cocaine was induced by an apoptotic mechanism, confirmed by TUNEL assay. Therefore, the present paper shows that cocaine causes apoptotic cell death and inhibition of the neurite prolongation in striatal and mesencephalic cell culture. These data suggest that if similar neuronal damage could be produced in the developing human brain, it could account for the qualitative or quantitative defects in neuronal pathways that cause a major handicap in brain function following prenatal exposure to cocaine.
publishDate 2015
dc.date.none.fl_str_mv 2015-01-01
2018-11-26T15:27:45Z
2018-11-26T15:27:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1155/2015/750752
Biomed Research International. New York: Hindawi Publishing Corporation, 7 p., 2015.
2314-6133
http://hdl.handle.net/11449/158457
10.1155/2015/750752
WOS:000359237100001
WOS000359237100001.pdf
2514762545280942
0000-0002-1378-6327
url http://dx.doi.org/10.1155/2015/750752
http://hdl.handle.net/11449/158457
identifier_str_mv Biomed Research International. New York: Hindawi Publishing Corporation, 7 p., 2015.
2314-6133
10.1155/2015/750752
WOS:000359237100001
WOS000359237100001.pdf
2514762545280942
0000-0002-1378-6327
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biomed Research International
0,935
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 7
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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