Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations

Detalhes bibliográficos
Autor(a) principal: Pinheiro Ferreira, Paulo Michel
Data de Publicação: 2016
Outros Autores: Bezerra, Daniel Pereira, Silva, Jurandy do Nascimento, Costa, Marcilia Pinheiro da, Oliveira Ferreira, Jose Roberto de, Nunes Alencar, Nylane Maria, Tavares de Figueiredo, Ingrid Samantha, Cavalheiro, Alberto Jose [UNESP], Longo Machado, Camila Maria [UNESP], Chammas, Roger [UNESP], Negreiros Nunes Alves, Ana Paula, Moraes, Manoel Odorico de, Pessoa, Claudia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jep.2016.04.011
http://hdl.handle.net/11449/165207
Resumo: Ethnopharmacological relevance: Casearia sylvestris (Salicaceae) is found in South America and presents antiulcerogenic, cytotoxic, antimicrobial, anti-inflammatory and antihypertensive activities. Aim of the study: To assess the in vivo and ex vivo antitumor action of a fraction with casearins (FC) and its main component- Casearin X-isolated from C sylvestris leaves. Materials and methods: Firstly, Sarcoma 180 bearing Swiss mice were treated with FC and Cas X for 7 days. Secondly, BALB/c nude animals received hollow fibers with colon carcinoma (HCT-116) or glioblastoma (SF-295) cells and were treated with FC for 4 days. On 5th day, proliferation was determined by MIT assay. Results: FC 10 and 25 mg/kg/day i.p. and 50 mg/kg/day oral and Cas X 25 mg/kg/day i.p. and 50 mg/kg/ day oral revealed tumor growth inhibition rates of 35.8, 86.2, 53.7, 90.0 and 65.5% and such tumors demonstrated rare mitoses and coagulation necrosis areas. Similarly, FC reduced multiplying of HCT-116 and SF-295 cells when evaluated by the Hollow Fiber Assay (2.5 and 5 mg/kg/day i.p. and 25 and 50 mg/ kg/day oral), with cell growth inhibition rates ranging from 33.3 to 67.4% (p < 0.05). Flow cytometry experiments revealed that FC reduced membrane integrity and induced DNA fragmentation and mitochondrial depolarization (p < 0.05). Conclusions: FC and Cas X were efficient antitumor substances against murine and human cancer cells and caused reversible morphological changes in liver, kidneys and spleens, emphasizing clerodane diterpenes as an emerging class of anticancer molecules. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
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spelling Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinationsCasearin XHollow fiber assaySarcoma 180AntineoplasticLeukogramToxicityEthnopharmacological relevance: Casearia sylvestris (Salicaceae) is found in South America and presents antiulcerogenic, cytotoxic, antimicrobial, anti-inflammatory and antihypertensive activities. Aim of the study: To assess the in vivo and ex vivo antitumor action of a fraction with casearins (FC) and its main component- Casearin X-isolated from C sylvestris leaves. Materials and methods: Firstly, Sarcoma 180 bearing Swiss mice were treated with FC and Cas X for 7 days. Secondly, BALB/c nude animals received hollow fibers with colon carcinoma (HCT-116) or glioblastoma (SF-295) cells and were treated with FC for 4 days. On 5th day, proliferation was determined by MIT assay. Results: FC 10 and 25 mg/kg/day i.p. and 50 mg/kg/day oral and Cas X 25 mg/kg/day i.p. and 50 mg/kg/ day oral revealed tumor growth inhibition rates of 35.8, 86.2, 53.7, 90.0 and 65.5% and such tumors demonstrated rare mitoses and coagulation necrosis areas. Similarly, FC reduced multiplying of HCT-116 and SF-295 cells when evaluated by the Hollow Fiber Assay (2.5 and 5 mg/kg/day i.p. and 25 and 50 mg/ kg/day oral), with cell growth inhibition rates ranging from 33.3 to 67.4% (p < 0.05). Flow cytometry experiments revealed that FC reduced membrane integrity and induced DNA fragmentation and mitochondrial depolarization (p < 0.05). Conclusions: FC and Cas X were efficient antitumor substances against murine and human cancer cells and caused reversible morphological changes in liver, kidneys and spleens, emphasizing clerodane diterpenes as an emerging class of anticancer molecules. (C) 2016 Elsevier Ireland Ltd. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Piaui, Dept Physiol & Biophys, Expt Cancerol Lab, Teresina, BrazilUniv Fed Piaui, Postgrad Program Pharmaceut Sci, Teresina, BrazilUniv Fed Piaui, Postgrad Program Biotechnol, Teresina, BrazilFundacao Oswaldo Cruz, Salvador, BA, BrazilUniv Fed Piaui, Dept Pharm, Teresina, BrazilUniv Fed Ceara, Fac Med, Dept Physiol & Pharmacol, Fortaleza, Ceara, BrazilState Univ Sao Paulo, Inst Chem, Araraquara, BrazilState Univ Sao Paulo, Ctr Med Nucl, Radioisotopes Res Lab, Sao Paulo, BrazilState Univ Sao Paulo, Fac Med, Dept Radiol, Sao Paulo, BrazilUniv Fed Ceara, Dept Clin Odontol, Fortaleza, Ceara, BrazilFundacao Oswaldo Cruz, Fortaleza, Ceara, BrazilState Univ Sao Paulo, Inst Chem, Araraquara, BrazilState Univ Sao Paulo, Ctr Med Nucl, Radioisotopes Res Lab, Sao Paulo, BrazilState Univ Sao Paulo, Fac Med, Dept Radiol, Sao Paulo, BrazilCNPq: 473167/2012-3CNPq: 301976/2013-9Elsevier B.V.Univ Fed PiauiFundacao Oswaldo CruzUniv Fed CearaUniversidade Estadual Paulista (Unesp)Pinheiro Ferreira, Paulo MichelBezerra, Daniel PereiraSilva, Jurandy do NascimentoCosta, Marcilia Pinheiro daOliveira Ferreira, Jose Roberto deNunes Alencar, Nylane MariaTavares de Figueiredo, Ingrid SamanthaCavalheiro, Alberto Jose [UNESP]Longo Machado, Camila Maria [UNESP]Chammas, Roger [UNESP]Negreiros Nunes Alves, Ana PaulaMoraes, Manoel Odorico dePessoa, Claudia2018-11-27T16:55:43Z2018-11-27T16:55:43Z2016-06-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article270-279application/pdfhttp://dx.doi.org/10.1016/j.jep.2016.04.011Journal Of Ethnopharmacology. Clare: Elsevier Ireland Ltd, v. 186, p. 270-279, 2016.0378-8741http://hdl.handle.net/11449/16520710.1016/j.jep.2016.04.011WOS:000377832000029WOS000377832000029.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal Of Ethnopharmacology1,150info:eu-repo/semantics/openAccess2023-10-07T06:04:36Zoai:repositorio.unesp.br:11449/165207Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-07T06:04:36Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations
title Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations
spellingShingle Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations
Pinheiro Ferreira, Paulo Michel
Casearin X
Hollow fiber assay
Sarcoma 180
Antineoplastic
Leukogram
Toxicity
title_short Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations
title_full Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations
title_fullStr Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations
title_full_unstemmed Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations
title_sort Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations
author Pinheiro Ferreira, Paulo Michel
author_facet Pinheiro Ferreira, Paulo Michel
Bezerra, Daniel Pereira
Silva, Jurandy do Nascimento
Costa, Marcilia Pinheiro da
Oliveira Ferreira, Jose Roberto de
Nunes Alencar, Nylane Maria
Tavares de Figueiredo, Ingrid Samantha
Cavalheiro, Alberto Jose [UNESP]
Longo Machado, Camila Maria [UNESP]
Chammas, Roger [UNESP]
Negreiros Nunes Alves, Ana Paula
Moraes, Manoel Odorico de
Pessoa, Claudia
author_role author
author2 Bezerra, Daniel Pereira
Silva, Jurandy do Nascimento
Costa, Marcilia Pinheiro da
Oliveira Ferreira, Jose Roberto de
Nunes Alencar, Nylane Maria
Tavares de Figueiredo, Ingrid Samantha
Cavalheiro, Alberto Jose [UNESP]
Longo Machado, Camila Maria [UNESP]
Chammas, Roger [UNESP]
Negreiros Nunes Alves, Ana Paula
Moraes, Manoel Odorico de
Pessoa, Claudia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Univ Fed Piaui
Fundacao Oswaldo Cruz
Univ Fed Ceara
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Pinheiro Ferreira, Paulo Michel
Bezerra, Daniel Pereira
Silva, Jurandy do Nascimento
Costa, Marcilia Pinheiro da
Oliveira Ferreira, Jose Roberto de
Nunes Alencar, Nylane Maria
Tavares de Figueiredo, Ingrid Samantha
Cavalheiro, Alberto Jose [UNESP]
Longo Machado, Camila Maria [UNESP]
Chammas, Roger [UNESP]
Negreiros Nunes Alves, Ana Paula
Moraes, Manoel Odorico de
Pessoa, Claudia
dc.subject.por.fl_str_mv Casearin X
Hollow fiber assay
Sarcoma 180
Antineoplastic
Leukogram
Toxicity
topic Casearin X
Hollow fiber assay
Sarcoma 180
Antineoplastic
Leukogram
Toxicity
description Ethnopharmacological relevance: Casearia sylvestris (Salicaceae) is found in South America and presents antiulcerogenic, cytotoxic, antimicrobial, anti-inflammatory and antihypertensive activities. Aim of the study: To assess the in vivo and ex vivo antitumor action of a fraction with casearins (FC) and its main component- Casearin X-isolated from C sylvestris leaves. Materials and methods: Firstly, Sarcoma 180 bearing Swiss mice were treated with FC and Cas X for 7 days. Secondly, BALB/c nude animals received hollow fibers with colon carcinoma (HCT-116) or glioblastoma (SF-295) cells and were treated with FC for 4 days. On 5th day, proliferation was determined by MIT assay. Results: FC 10 and 25 mg/kg/day i.p. and 50 mg/kg/day oral and Cas X 25 mg/kg/day i.p. and 50 mg/kg/ day oral revealed tumor growth inhibition rates of 35.8, 86.2, 53.7, 90.0 and 65.5% and such tumors demonstrated rare mitoses and coagulation necrosis areas. Similarly, FC reduced multiplying of HCT-116 and SF-295 cells when evaluated by the Hollow Fiber Assay (2.5 and 5 mg/kg/day i.p. and 25 and 50 mg/ kg/day oral), with cell growth inhibition rates ranging from 33.3 to 67.4% (p < 0.05). Flow cytometry experiments revealed that FC reduced membrane integrity and induced DNA fragmentation and mitochondrial depolarization (p < 0.05). Conclusions: FC and Cas X were efficient antitumor substances against murine and human cancer cells and caused reversible morphological changes in liver, kidneys and spleens, emphasizing clerodane diterpenes as an emerging class of anticancer molecules. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
publishDate 2016
dc.date.none.fl_str_mv 2016-06-20
2018-11-27T16:55:43Z
2018-11-27T16:55:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jep.2016.04.011
Journal Of Ethnopharmacology. Clare: Elsevier Ireland Ltd, v. 186, p. 270-279, 2016.
0378-8741
http://hdl.handle.net/11449/165207
10.1016/j.jep.2016.04.011
WOS:000377832000029
WOS000377832000029.pdf
url http://dx.doi.org/10.1016/j.jep.2016.04.011
http://hdl.handle.net/11449/165207
identifier_str_mv Journal Of Ethnopharmacology. Clare: Elsevier Ireland Ltd, v. 186, p. 270-279, 2016.
0378-8741
10.1016/j.jep.2016.04.011
WOS:000377832000029
WOS000377832000029.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal Of Ethnopharmacology
1,150
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 270-279
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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