Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/18081 |
Resumo: | Federal Brazilian agency “Conselho Nacional de Desenvolvimento Científico e Tecnológico” (CNPq) for financial supportin the form of grants (#473167/2012-3) and fellowship (#301976/2013-9) |
| id |
CRUZ_a2cb9623638bf9530a3fbb5f450d068b |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/18081 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Ferreira, Paulo Michel PinheiroBezerra, Daniel PereiraSilva, Jurandy do NascimentoCosta, Marcília Pinheiro daFerreira, José Roberto de OliveiraAlencar, Nylane Maria NunesFigueiredo, Ingrid Samantha Tavares deCavalheiro, Alberto JoséMachado, Camila Maria LongoChammas, RogerAlves, Ana Paula Negreiros NunesMoraes, Manoel Odorico dePessoa, Claudia2017-03-20T17:15:41Z2017-03-20T17:15:41Z2016FERREIRA, P. M. P. et al. Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations. Journal of Ethnopharmacology, v. 186, p. 270–279, 2016.0378-8741https://www.arca.fiocruz.br/handle/icict/1808110.1016/j.jep.2016.04.011Federal Brazilian agency “Conselho Nacional de Desenvolvimento Científico e Tecnológico” (CNPq) for financial supportin the form of grants (#473167/2012-3) and fellowship (#301976/2013-9)Federal University of Piauí. Laboratory in Experimental Cancerology. Department of Biophysics and Physiology.Teresina, PI, Brazil / Federal University of Piauí. Postgraduate Programin Pharmaceutical Sciences. Teresina, PI, Brazil / Federal University of Piauí. Postgraduate Programin in Biotechnology. Teresina, PI, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFederal University of Piauí. Postgraduate Programin in Biotechnology. Teresina, PI, BrazilFederal University of Piauí. Postgraduate Programin Pharmaceutical Sciences. Teresina, PI, Brazil / Federal University of Piauí. Department of Pharmacy. Teresina, PI, BrazilFederal University of Piauí. Postgraduate Programin Pharmaceutical Sciences. Teresina, PI, BrazilFederal University of Ceará. Faculty of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, BrazilFederal University of Ceará. Faculty of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, BrazilState University of São Paulo. Chemistry Institute. Araraquara, SP, BrazilState University of SãoPaulo. Center for Medical Nuclear. Radioisotopes Research Laboratory. São Paulo, SP, BrazilState University of Paulo. São Faculty of Medicine. Department of Radiology. São Paulo, SP, BrazilFederal University of Ceará. Department of Clinical Odontology. Fortaleza, CE, BrazilFederal University of Ceará. Faculty of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, BrazilFederal University of Ceará. Faculty of Medicine. Department of Physiology and Pharmacology. Fortaleza, CE, Brazil / Oswaldo Cruz Foundation. Fortaleza, CE, BrazilCasearia sylvestris (Salicaceae) is found in South America and presents antiulcerogenic, cytotoxic, antimicrobial, anti-inflammatory and antihypertensive activities. Aim ofthestudy: Toassessthe in vivoandexvivo antitumor actionofafractionwithcasearins(FC)andits main component-CasearinX-isolatedfrom C. sylvestris leaves. Materials andmethods: Firstly,Sarcoma180bearingSwissmiceweretreatedwithFCandCasXfor 7 days.Secondly,BALB/cnudeanimalsreceivedhollow fibers withcoloncarcinoma(HCT-116)orglio- blastoma (SF-295)cellsandweretreatedwithFCfor4days.On5thday,proliferationwasdeterminedby MTT assay. Results: FC 10and25mg/kg/dayi.p.and50mg/kg/dayoralandCasX25mg/kg/dayi.p.and50mg/kg/ day oralrevealedtumorgrowthinhibitionratesof35.8,86.2,53.7,90.0and65.5%andsuchtumors demonstratedraremitosesandcoagulationnecrosisareas.Similarly,FCreducedmultiplyingofHCT-116 and SF-295cellswhenevaluatedbytheHollowFiberAssay(2.5and5mg/kg/dayi.p.and25and50mg/ kg/day oral),withcellgrowthinhibitionratesrangingfrom33.3to67.4%(po0.05). Flowcytometry experimentsrevealedthatFCreducedmembraneintegrityandinducedDNAfragmentationandmi- tochondrial depolarization(po0.05). Conclusions: FC andCasXwereefficient antitumorsubstancesagainstmurineandhumancancercells and causedreversiblemorphologicalchangesinliver,kidneysandspleens,emphasizingclerodanedi- terpenes asanemergingclassofanticancermolecules.engElsevierCasearin XTeste de fibra ocaSarcoma 180LeucogramaAntineoplásicoCasearin XHollow fiber assaySarcoma 180AntineoplasticLeukogramPreclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18081/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFerreira PMP Preclinical anticancer....pdfFerreira PMP Preclinical anticancer....pdfapplication/pdf2237873https://www.arca.fiocruz.br/bitstream/icict/18081/2/Ferreira%20PMP%20Preclinical%20anticancer....pdfa782fd405e610e2b72306305766c0c3bMD52TEXTFerreira PMP Preclinical anticancer....pdf.txtFerreira PMP Preclinical anticancer....pdf.txtExtracted texttext/plain56786https://www.arca.fiocruz.br/bitstream/icict/18081/3/Ferreira%20PMP%20Preclinical%20anticancer....pdf.txtf1cfb942726cacf4ff075e54ac8d0631MD53icict/180812023-03-15 14:33:28.941oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:28Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations |
| title |
Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations |
| spellingShingle |
Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations Ferreira, Paulo Michel Pinheiro Casearin X Teste de fibra oca Sarcoma 180 Leucograma Antineoplásico Casearin X Hollow fiber assay Sarcoma 180 Antineoplastic Leukogram |
| title_short |
Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations |
| title_full |
Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations |
| title_fullStr |
Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations |
| title_full_unstemmed |
Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations |
| title_sort |
Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations |
| author |
Ferreira, Paulo Michel Pinheiro |
| author_facet |
Ferreira, Paulo Michel Pinheiro Bezerra, Daniel Pereira Silva, Jurandy do Nascimento Costa, Marcília Pinheiro da Ferreira, José Roberto de Oliveira Alencar, Nylane Maria Nunes Figueiredo, Ingrid Samantha Tavares de Cavalheiro, Alberto José Machado, Camila Maria Longo Chammas, Roger Alves, Ana Paula Negreiros Nunes Moraes, Manoel Odorico de Pessoa, Claudia |
| author_role |
author |
| author2 |
Bezerra, Daniel Pereira Silva, Jurandy do Nascimento Costa, Marcília Pinheiro da Ferreira, José Roberto de Oliveira Alencar, Nylane Maria Nunes Figueiredo, Ingrid Samantha Tavares de Cavalheiro, Alberto José Machado, Camila Maria Longo Chammas, Roger Alves, Ana Paula Negreiros Nunes Moraes, Manoel Odorico de Pessoa, Claudia |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Ferreira, Paulo Michel Pinheiro Bezerra, Daniel Pereira Silva, Jurandy do Nascimento Costa, Marcília Pinheiro da Ferreira, José Roberto de Oliveira Alencar, Nylane Maria Nunes Figueiredo, Ingrid Samantha Tavares de Cavalheiro, Alberto José Machado, Camila Maria Longo Chammas, Roger Alves, Ana Paula Negreiros Nunes Moraes, Manoel Odorico de Pessoa, Claudia |
| dc.subject.other.pt_BR.fl_str_mv |
Casearin X Teste de fibra oca Sarcoma 180 Leucograma Antineoplásico |
| topic |
Casearin X Teste de fibra oca Sarcoma 180 Leucograma Antineoplásico Casearin X Hollow fiber assay Sarcoma 180 Antineoplastic Leukogram |
| dc.subject.en.pt_BR.fl_str_mv |
Casearin X Hollow fiber assay Sarcoma 180 Antineoplastic Leukogram |
| description |
Federal Brazilian agency “Conselho Nacional de Desenvolvimento Científico e Tecnológico” (CNPq) for financial supportin the form of grants (#473167/2012-3) and fellowship (#301976/2013-9) |
| publishDate |
2016 |
| dc.date.issued.fl_str_mv |
2016 |
| dc.date.accessioned.fl_str_mv |
2017-03-20T17:15:41Z |
| dc.date.available.fl_str_mv |
2017-03-20T17:15:41Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
FERREIRA, P. M. P. et al. Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations. Journal of Ethnopharmacology, v. 186, p. 270–279, 2016. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/18081 |
| dc.identifier.issn.pt_BR.fl_str_mv |
0378-8741 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.jep.2016.04.011 |
| identifier_str_mv |
FERREIRA, P. M. P. et al. Preclinical anticancer effectiveness of a fraction from Casearia sylvestris and its component Casearin X: in vivo and ex vivo methods and microscopy examinations. Journal of Ethnopharmacology, v. 186, p. 270–279, 2016. 0378-8741 10.1016/j.jep.2016.04.011 |
| url |
https://www.arca.fiocruz.br/handle/icict/18081 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Elsevier |
| publisher.none.fl_str_mv |
Elsevier |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/18081/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/18081/2/Ferreira%20PMP%20Preclinical%20anticancer....pdf https://www.arca.fiocruz.br/bitstream/icict/18081/3/Ferreira%20PMP%20Preclinical%20anticancer....pdf.txt |
| bitstream.checksum.fl_str_mv |
5a560609d32a3863062d77ff32785d58 a782fd405e610e2b72306305766c0c3b f1cfb942726cacf4ff075e54ac8d0631 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1813009075524337664 |