Collagen I and III ratios and tenacity of rats’ muscle injured and treated with platelet-rich plasma

Detalhes bibliográficos
Autor(a) principal: Garcia, Thiago Alves
Data de Publicação: 2020
Outros Autores: Ozaki, Guilherme Akio Tamura [UNESP], Castoldi, Robson Chacon, Job, Aldo Eloizo [UNESP], Camargo, Regina Celi Trindade [UNESP], Filho, José Carlos Silva Camargo [UNESP], Belangero, William Dias
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.4067/S0717-95022020000501392
http://hdl.handle.net/11449/228840
Resumo: Severe muscle injuries are common in accidents and have a delayed recovery of muscle integrity. In these cases, muscle suture surgery is the standard treatment. However, Platelet Rich Plasma (PRP), has been widely used in orthopedic injuries due to its growth factors. Thus, the objective of the study will be to analyze the association of suture and PRP techniques in the collagen and tenacity of the injured muscle. Were used seventy rats, divided into five groups: control (C), injury control (CI), injury and suture (IS), injury and PRP (IP), injury, suture, and PRP (ISP). Were sectioned approximately 50 % of the width and 100 % of the thickness of the gastrocnemius muscle. The homologous PRP was applied 24h after the injury. On the 7th day after the injury, the animals were euthanized and their muscles subjected to mechanical testing to measure tenacity or collagen analysis to calculate the ratio between type I and III collagen. The results show a significant decrease (p <0.05) in the values of the relationship between collagens in all injured groups (CI, IS, IP, ISP) compared to group C. In injured groups, the tenacity was significantly (p <0.05) reduced compared to the control group, with no observed difference between treatments and injured groups. The amount of collagen in the injured area has increased, but it did not affect the tenacity of the muscles, which was reduced.
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spelling Collagen I and III ratios and tenacity of rats’ muscle injured and treated with platelet-rich plasmaRelaciones de colágeno I y III y tenacidad del músculo de rata lesionado y tratado con plasma rico en plaquetasCollagenMuscle injuryPlatelet-rich plasmaSutureSevere muscle injuries are common in accidents and have a delayed recovery of muscle integrity. In these cases, muscle suture surgery is the standard treatment. However, Platelet Rich Plasma (PRP), has been widely used in orthopedic injuries due to its growth factors. Thus, the objective of the study will be to analyze the association of suture and PRP techniques in the collagen and tenacity of the injured muscle. Were used seventy rats, divided into five groups: control (C), injury control (CI), injury and suture (IS), injury and PRP (IP), injury, suture, and PRP (ISP). Were sectioned approximately 50 % of the width and 100 % of the thickness of the gastrocnemius muscle. The homologous PRP was applied 24h after the injury. On the 7th day after the injury, the animals were euthanized and their muscles subjected to mechanical testing to measure tenacity or collagen analysis to calculate the ratio between type I and III collagen. The results show a significant decrease (p <0.05) in the values of the relationship between collagens in all injured groups (CI, IS, IP, ISP) compared to group C. In injured groups, the tenacity was significantly (p <0.05) reduced compared to the control group, with no observed difference between treatments and injured groups. The amount of collagen in the injured area has increased, but it did not affect the tenacity of the muscles, which was reduced.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratório de Biomateriais (LABIMO) do Centro de Medicina Experimental e Cirurgia Faculdade de Ciências Médicas Universidade Estadual de CampinasDepartamento de Fisioterapia Universidade Estadual Paulista (UNIP), Campus Araçatuba,Departamento de Educação Física Universidade do Oeste Paulista, Campus Presidente PrudenteDepartamento de Física Universidade Estadual Paulista “Júlio de Mesquita Filho”, Campus Presidente PrudenteDepartamento de Fisioterapia Universidade Estadual Paulista “Júlio de Mesquita Filho”, Campus Presidente PrudenteDepartamento de Ortopedia e Traumatologia da Faculdade de Ciências Médicas da Universidade Estadual de CampinasDepartamento de Fisioterapia Universidade Estadual Paulista (UNIP), Campus Araçatuba,Departamento de Física Universidade Estadual Paulista “Júlio de Mesquita Filho”, Campus Presidente PrudenteDepartamento de Fisioterapia Universidade Estadual Paulista “Júlio de Mesquita Filho”, Campus Presidente PrudenteFAPESP: 2017/ 06930-0Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (UNESP)Universidade do Oeste PaulistaGarcia, Thiago AlvesOzaki, Guilherme Akio Tamura [UNESP]Castoldi, Robson ChaconJob, Aldo Eloizo [UNESP]Camargo, Regina Celi Trindade [UNESP]Filho, José Carlos Silva Camargo [UNESP]Belangero, William Dias2022-04-29T08:28:57Z2022-04-29T08:28:57Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1392-1397http://dx.doi.org/10.4067/S0717-95022020000501392International Journal of Morphology, v. 38, n. 5, p. 1392-1397, 2020.0717-95020717-9367http://hdl.handle.net/11449/22884010.4067/S0717-950220200005013922-s2.0-85089855033Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Morphologyinfo:eu-repo/semantics/openAccess2024-06-19T12:44:05Zoai:repositorio.unesp.br:11449/228840Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:56:19.354620Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Collagen I and III ratios and tenacity of rats’ muscle injured and treated with platelet-rich plasma
Relaciones de colágeno I y III y tenacidad del músculo de rata lesionado y tratado con plasma rico en plaquetas
title Collagen I and III ratios and tenacity of rats’ muscle injured and treated with platelet-rich plasma
spellingShingle Collagen I and III ratios and tenacity of rats’ muscle injured and treated with platelet-rich plasma
Garcia, Thiago Alves
Collagen
Muscle injury
Platelet-rich plasma
Suture
title_short Collagen I and III ratios and tenacity of rats’ muscle injured and treated with platelet-rich plasma
title_full Collagen I and III ratios and tenacity of rats’ muscle injured and treated with platelet-rich plasma
title_fullStr Collagen I and III ratios and tenacity of rats’ muscle injured and treated with platelet-rich plasma
title_full_unstemmed Collagen I and III ratios and tenacity of rats’ muscle injured and treated with platelet-rich plasma
title_sort Collagen I and III ratios and tenacity of rats’ muscle injured and treated with platelet-rich plasma
author Garcia, Thiago Alves
author_facet Garcia, Thiago Alves
Ozaki, Guilherme Akio Tamura [UNESP]
Castoldi, Robson Chacon
Job, Aldo Eloizo [UNESP]
Camargo, Regina Celi Trindade [UNESP]
Filho, José Carlos Silva Camargo [UNESP]
Belangero, William Dias
author_role author
author2 Ozaki, Guilherme Akio Tamura [UNESP]
Castoldi, Robson Chacon
Job, Aldo Eloizo [UNESP]
Camargo, Regina Celi Trindade [UNESP]
Filho, José Carlos Silva Camargo [UNESP]
Belangero, William Dias
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual Paulista (UNESP)
Universidade do Oeste Paulista
dc.contributor.author.fl_str_mv Garcia, Thiago Alves
Ozaki, Guilherme Akio Tamura [UNESP]
Castoldi, Robson Chacon
Job, Aldo Eloizo [UNESP]
Camargo, Regina Celi Trindade [UNESP]
Filho, José Carlos Silva Camargo [UNESP]
Belangero, William Dias
dc.subject.por.fl_str_mv Collagen
Muscle injury
Platelet-rich plasma
Suture
topic Collagen
Muscle injury
Platelet-rich plasma
Suture
description Severe muscle injuries are common in accidents and have a delayed recovery of muscle integrity. In these cases, muscle suture surgery is the standard treatment. However, Platelet Rich Plasma (PRP), has been widely used in orthopedic injuries due to its growth factors. Thus, the objective of the study will be to analyze the association of suture and PRP techniques in the collagen and tenacity of the injured muscle. Were used seventy rats, divided into five groups: control (C), injury control (CI), injury and suture (IS), injury and PRP (IP), injury, suture, and PRP (ISP). Were sectioned approximately 50 % of the width and 100 % of the thickness of the gastrocnemius muscle. The homologous PRP was applied 24h after the injury. On the 7th day after the injury, the animals were euthanized and their muscles subjected to mechanical testing to measure tenacity or collagen analysis to calculate the ratio between type I and III collagen. The results show a significant decrease (p <0.05) in the values of the relationship between collagens in all injured groups (CI, IS, IP, ISP) compared to group C. In injured groups, the tenacity was significantly (p <0.05) reduced compared to the control group, with no observed difference between treatments and injured groups. The amount of collagen in the injured area has increased, but it did not affect the tenacity of the muscles, which was reduced.
publishDate 2020
dc.date.none.fl_str_mv 2020-10-01
2022-04-29T08:28:57Z
2022-04-29T08:28:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4067/S0717-95022020000501392
International Journal of Morphology, v. 38, n. 5, p. 1392-1397, 2020.
0717-9502
0717-9367
http://hdl.handle.net/11449/228840
10.4067/S0717-95022020000501392
2-s2.0-85089855033
url http://dx.doi.org/10.4067/S0717-95022020000501392
http://hdl.handle.net/11449/228840
identifier_str_mv International Journal of Morphology, v. 38, n. 5, p. 1392-1397, 2020.
0717-9502
0717-9367
10.4067/S0717-95022020000501392
2-s2.0-85089855033
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Morphology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1392-1397
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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