Identification of Cryptosporidium bat genotypes XVI–XVIII in bats from Brazil
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00436-019-06342-6 http://hdl.handle.net/11449/190332 |
Resumo: | Cryptosporidiosis is an emergent zoonotic disease caused by the globally distributed protozoa Cryptosporidium spp. Although several Cryptosporidium studies related to humans and many animal species have been published, there are still limited studies on the epidemiology of Cryptosporidium infection in bats. The aim of this study was to determine the occurrence of Cryptosporidium spp. and to perform the molecular characterization of Cryptosporidium species and genotypes in fecal samples from bats in an urban area of the municipality of Araçatuba, state of São Paulo, Brazil. Nested PCR targeting the 18S rRNA, actin, and HSP-70 genes was performed to screen 141 fecal samples from bats and detected Cryptosporidium spp. in 16.3% (23/141) of the samples. Bidirectional sequencing identified three novel Cryptosporidium bat genotypes (XVI, XVII, and XVIII) and a new genotype (18SH) genetically similar to Cryptosporidium avium in six species of bats. This is the first report on the occurrence and molecular characterization of Cryptosporidium spp. in Brazilian bats. Zoonotic Cryptosporidium species were not found in fecal samples from bats living in an urban area in the municipality of Araçatuba, state of São Paulo, Brazil. |
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Identification of Cryptosporidium bat genotypes XVI–XVIII in bats from BrazilBrazilChiropteranCryptosporidial infectionMolecular characterizationCryptosporidiosis is an emergent zoonotic disease caused by the globally distributed protozoa Cryptosporidium spp. Although several Cryptosporidium studies related to humans and many animal species have been published, there are still limited studies on the epidemiology of Cryptosporidium infection in bats. The aim of this study was to determine the occurrence of Cryptosporidium spp. and to perform the molecular characterization of Cryptosporidium species and genotypes in fecal samples from bats in an urban area of the municipality of Araçatuba, state of São Paulo, Brazil. Nested PCR targeting the 18S rRNA, actin, and HSP-70 genes was performed to screen 141 fecal samples from bats and detected Cryptosporidium spp. in 16.3% (23/141) of the samples. Bidirectional sequencing identified three novel Cryptosporidium bat genotypes (XVI, XVII, and XVIII) and a new genotype (18SH) genetically similar to Cryptosporidium avium in six species of bats. This is the first report on the occurrence and molecular characterization of Cryptosporidium spp. in Brazilian bats. Zoonotic Cryptosporidium species were not found in fecal samples from bats living in an urban area in the municipality of Araçatuba, state of São Paulo, Brazil.Faculdade de Medicina Veterinária Universidade Estadual Paulista (UNESP) Araçatuba, Clóvis Pestana St., 793Faculdade de Medicina Veterinária Universidade Estadual Paulista (UNESP) Araçatuba, Clóvis Pestana St., 793Universidade Estadual Paulista (Unesp)Batista, Juliana Maria N. [UNESP]de Carvalho, Cristiano [UNESP]Pedro, Wagner A. [UNESP]Santana, Bruna N. [UNESP]Camargo, Vinícius S. [UNESP]Ferrari, Elis D. [UNESP]Nascimento, Isabela G. [UNESP]Meireles, Marcelo V. [UNESP]2019-10-06T17:09:43Z2019-10-06T17:09:43Z2019-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2183-2191http://dx.doi.org/10.1007/s00436-019-06342-6Parasitology Research, v. 118, n. 7, p. 2183-2191, 2019.1432-19550932-0113http://hdl.handle.net/11449/19033210.1007/s00436-019-06342-62-s2.0-85065726726Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasitology Researchinfo:eu-repo/semantics/openAccess2024-09-04T19:14:49Zoai:repositorio.unesp.br:11449/190332Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:14:49Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Identification of Cryptosporidium bat genotypes XVI–XVIII in bats from Brazil |
title |
Identification of Cryptosporidium bat genotypes XVI–XVIII in bats from Brazil |
spellingShingle |
Identification of Cryptosporidium bat genotypes XVI–XVIII in bats from Brazil Batista, Juliana Maria N. [UNESP] Brazil Chiropteran Cryptosporidial infection Molecular characterization |
title_short |
Identification of Cryptosporidium bat genotypes XVI–XVIII in bats from Brazil |
title_full |
Identification of Cryptosporidium bat genotypes XVI–XVIII in bats from Brazil |
title_fullStr |
Identification of Cryptosporidium bat genotypes XVI–XVIII in bats from Brazil |
title_full_unstemmed |
Identification of Cryptosporidium bat genotypes XVI–XVIII in bats from Brazil |
title_sort |
Identification of Cryptosporidium bat genotypes XVI–XVIII in bats from Brazil |
author |
Batista, Juliana Maria N. [UNESP] |
author_facet |
Batista, Juliana Maria N. [UNESP] de Carvalho, Cristiano [UNESP] Pedro, Wagner A. [UNESP] Santana, Bruna N. [UNESP] Camargo, Vinícius S. [UNESP] Ferrari, Elis D. [UNESP] Nascimento, Isabela G. [UNESP] Meireles, Marcelo V. [UNESP] |
author_role |
author |
author2 |
de Carvalho, Cristiano [UNESP] Pedro, Wagner A. [UNESP] Santana, Bruna N. [UNESP] Camargo, Vinícius S. [UNESP] Ferrari, Elis D. [UNESP] Nascimento, Isabela G. [UNESP] Meireles, Marcelo V. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Batista, Juliana Maria N. [UNESP] de Carvalho, Cristiano [UNESP] Pedro, Wagner A. [UNESP] Santana, Bruna N. [UNESP] Camargo, Vinícius S. [UNESP] Ferrari, Elis D. [UNESP] Nascimento, Isabela G. [UNESP] Meireles, Marcelo V. [UNESP] |
dc.subject.por.fl_str_mv |
Brazil Chiropteran Cryptosporidial infection Molecular characterization |
topic |
Brazil Chiropteran Cryptosporidial infection Molecular characterization |
description |
Cryptosporidiosis is an emergent zoonotic disease caused by the globally distributed protozoa Cryptosporidium spp. Although several Cryptosporidium studies related to humans and many animal species have been published, there are still limited studies on the epidemiology of Cryptosporidium infection in bats. The aim of this study was to determine the occurrence of Cryptosporidium spp. and to perform the molecular characterization of Cryptosporidium species and genotypes in fecal samples from bats in an urban area of the municipality of Araçatuba, state of São Paulo, Brazil. Nested PCR targeting the 18S rRNA, actin, and HSP-70 genes was performed to screen 141 fecal samples from bats and detected Cryptosporidium spp. in 16.3% (23/141) of the samples. Bidirectional sequencing identified three novel Cryptosporidium bat genotypes (XVI, XVII, and XVIII) and a new genotype (18SH) genetically similar to Cryptosporidium avium in six species of bats. This is the first report on the occurrence and molecular characterization of Cryptosporidium spp. in Brazilian bats. Zoonotic Cryptosporidium species were not found in fecal samples from bats living in an urban area in the municipality of Araçatuba, state of São Paulo, Brazil. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T17:09:43Z 2019-10-06T17:09:43Z 2019-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00436-019-06342-6 Parasitology Research, v. 118, n. 7, p. 2183-2191, 2019. 1432-1955 0932-0113 http://hdl.handle.net/11449/190332 10.1007/s00436-019-06342-6 2-s2.0-85065726726 |
url |
http://dx.doi.org/10.1007/s00436-019-06342-6 http://hdl.handle.net/11449/190332 |
identifier_str_mv |
Parasitology Research, v. 118, n. 7, p. 2183-2191, 2019. 1432-1955 0932-0113 10.1007/s00436-019-06342-6 2-s2.0-85065726726 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Parasitology Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2183-2191 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1810021357365231616 |