Different saturated and monounsaturated fatty acids levels in fish oil-free diets to cobia (Rachycentron canadum) juveniles: Effects in growth performance and lipid metabolism

Detalhes bibliográficos
Autor(a) principal: Marques, Victor H.
Data de Publicação: 2021
Outros Autores: Moreira, Renata G., Branco, Giovana S., Honji, Renato M., Rombenso, Artur N., Viana, Maria Teresa, Mello, Paulo H. de [UNESP], Mata-Sotres, Jose A., Araujo, Bruno C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.aquaculture.2021.736843
http://hdl.handle.net/11449/210396
Resumo: This study aimed to investigate the influences of alternative lipid sources rich in saturated and monounsaturated fatty acids (SFA and MUFA) supplemented with long-chain polyunsaturated fatty acids (LC-PUFA) on production performance and lipid metabolism of Rachycentron canadum (cobia) juveniles. An 8-week feeding trial was carried out using four isoproteic and isolipidic diets as follows: FO-D (fish oil, as control diet), SFA-D (rich in SFA), MIX-D (same levels of SFA and MUFA), and MUFA-D (rich in MUFA). Experimental diets were supplemented with arachidonic acid (ARA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) (3, 5, and 10 g kg(1), respectively). The growth performance, fatty acid (FA) profile of liver and muscle, hepatocyte morphology, and gene expression related to the FA synthesis and oxidation on the liver were examined. In general, production performance was not impaired in fish-fed FO-free diets, supporting the hypothesis that alternative lipid sources could be used in cobia's aquafeed formulations when the LC-PUFA are adequately supplemented. High dietary SFA levels were disproportionally deposited the liver and muscle. Contrariwise MUFA was mainly deposited in the liver and muscle, reflecting the dietary inclusion levels. The main FA influencing this pattern were 12:0 and 18:1n-9. The expression of fatty acid synthase (fas) was up-regulated in the FO-D group compared to SFA-D and MIX-D groups. There were no differences in the relative expressions of carnitine palmitoyltransferase 1 (cpt-1 alpha) and lipase lipoprotein (lpl). The liver morphology results indicated that fish-fed SFA-D presented a smaller lipid vacuoles area than those fed other experimental diets. This study shows that SFA with shorter carbon chains such as 12:0 can be administered in cobia aquafeeds to stimulate these molecules' catabolism, providing energy for growth, and retaining LC-PUFAs in tissues, especially in the muscle, exhibiting a healthier fillet for consumers.
id UNSP_ee0eb6f7b9da6831b983beb991659bea
oai_identifier_str oai:repositorio.unesp.br:11449/210396
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Different saturated and monounsaturated fatty acids levels in fish oil-free diets to cobia (Rachycentron canadum) juveniles: Effects in growth performance and lipid metabolismFatty acidsAlternative lipidsSFAMUFAbeta-OxidationThis study aimed to investigate the influences of alternative lipid sources rich in saturated and monounsaturated fatty acids (SFA and MUFA) supplemented with long-chain polyunsaturated fatty acids (LC-PUFA) on production performance and lipid metabolism of Rachycentron canadum (cobia) juveniles. An 8-week feeding trial was carried out using four isoproteic and isolipidic diets as follows: FO-D (fish oil, as control diet), SFA-D (rich in SFA), MIX-D (same levels of SFA and MUFA), and MUFA-D (rich in MUFA). Experimental diets were supplemented with arachidonic acid (ARA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) (3, 5, and 10 g kg(1), respectively). The growth performance, fatty acid (FA) profile of liver and muscle, hepatocyte morphology, and gene expression related to the FA synthesis and oxidation on the liver were examined. In general, production performance was not impaired in fish-fed FO-free diets, supporting the hypothesis that alternative lipid sources could be used in cobia's aquafeed formulations when the LC-PUFA are adequately supplemented. High dietary SFA levels were disproportionally deposited the liver and muscle. Contrariwise MUFA was mainly deposited in the liver and muscle, reflecting the dietary inclusion levels. The main FA influencing this pattern were 12:0 and 18:1n-9. The expression of fatty acid synthase (fas) was up-regulated in the FO-D group compared to SFA-D and MIX-D groups. There were no differences in the relative expressions of carnitine palmitoyltransferase 1 (cpt-1 alpha) and lipase lipoprotein (lpl). The liver morphology results indicated that fish-fed SFA-D presented a smaller lipid vacuoles area than those fed other experimental diets. This study shows that SFA with shorter carbon chains such as 12:0 can be administered in cobia aquafeeds to stimulate these molecules' catabolism, providing energy for growth, and retaining LC-PUFAs in tissues, especially in the muscle, exhibiting a healthier fillet for consumers.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Centro de Biologia Marinha da Universidade de Sao Paulo (CEBIMar)Univ Sao Paulo, Dept Fisiol, Inst Biociencias, Rua Matao,Trav 14,321, BR-05508090 Sao Paulo, SP, BrazilUniv Sao Paulo CEBIMar USP, Ctr Biol Marinha, Rodovia Manoel Hipolito Rego,Km 131,5, BR-11612109 Sao Sebastiao, SP, BrazilCSIRO Agr & Food, Livestock & Aquaculture Program, Bribie Isl Res Ctr, Woorim, Qld 4507, AustraliaUniv Autonoma Baja California UABC, Inst Invest Oceanol, Km 107 Carretera Tij Eda, Ensenada 22860, Baja California, MexicoUniv Estadual Paulista Julio de Mesquita Filho CA, Ctr Aquicultura, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilUABC, Inst Invest Oceanol, CONACYT, Ensenada, BC, MexicoUniv Mogi das Cruzes, Nucleo Integrado Biotecnol, Ave Dr Candido Xavier Almeida Souza 200, BR-08780911 Mogi Das Cruzes, SP, BrazilUniv Estadual Paulista Julio de Mesquita Filho CA, Ctr Aquicultura, Via Acesso Prof Paulo Donato Castellane S-N, BR-14884900 Jaboticabal, SP, BrazilFAPESP: 2019/092469FAPESP: 2014/163207FAPESP: 2017/067650FAPESP: 2019/260180Elsevier B.V.Universidade de São Paulo (USP)CSIRO Agr & FoodUniv Autonoma Baja California UABCUniversidade Estadual Paulista (Unesp)UABCUniv Mogi das CruzesMarques, Victor H.Moreira, Renata G.Branco, Giovana S.Honji, Renato M.Rombenso, Artur N.Viana, Maria TeresaMello, Paulo H. de [UNESP]Mata-Sotres, Jose A.Araujo, Bruno C.2021-06-25T15:07:11Z2021-06-25T15:07:11Z2021-08-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article12http://dx.doi.org/10.1016/j.aquaculture.2021.736843Aquaculture. Amsterdam: Elsevier, v. 541, 12 p., 2021.0044-8486http://hdl.handle.net/11449/21039610.1016/j.aquaculture.2021.736843WOS:000657609900006Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAquacultureinfo:eu-repo/semantics/openAccess2024-04-09T15:43:35Zoai:repositorio.unesp.br:11449/210396Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:35:08.539593Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Different saturated and monounsaturated fatty acids levels in fish oil-free diets to cobia (Rachycentron canadum) juveniles: Effects in growth performance and lipid metabolism
title Different saturated and monounsaturated fatty acids levels in fish oil-free diets to cobia (Rachycentron canadum) juveniles: Effects in growth performance and lipid metabolism
spellingShingle Different saturated and monounsaturated fatty acids levels in fish oil-free diets to cobia (Rachycentron canadum) juveniles: Effects in growth performance and lipid metabolism
Marques, Victor H.
Fatty acids
Alternative lipids
SFA
MUFA
beta-Oxidation
title_short Different saturated and monounsaturated fatty acids levels in fish oil-free diets to cobia (Rachycentron canadum) juveniles: Effects in growth performance and lipid metabolism
title_full Different saturated and monounsaturated fatty acids levels in fish oil-free diets to cobia (Rachycentron canadum) juveniles: Effects in growth performance and lipid metabolism
title_fullStr Different saturated and monounsaturated fatty acids levels in fish oil-free diets to cobia (Rachycentron canadum) juveniles: Effects in growth performance and lipid metabolism
title_full_unstemmed Different saturated and monounsaturated fatty acids levels in fish oil-free diets to cobia (Rachycentron canadum) juveniles: Effects in growth performance and lipid metabolism
title_sort Different saturated and monounsaturated fatty acids levels in fish oil-free diets to cobia (Rachycentron canadum) juveniles: Effects in growth performance and lipid metabolism
author Marques, Victor H.
author_facet Marques, Victor H.
Moreira, Renata G.
Branco, Giovana S.
Honji, Renato M.
Rombenso, Artur N.
Viana, Maria Teresa
Mello, Paulo H. de [UNESP]
Mata-Sotres, Jose A.
Araujo, Bruno C.
author_role author
author2 Moreira, Renata G.
Branco, Giovana S.
Honji, Renato M.
Rombenso, Artur N.
Viana, Maria Teresa
Mello, Paulo H. de [UNESP]
Mata-Sotres, Jose A.
Araujo, Bruno C.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
CSIRO Agr & Food
Univ Autonoma Baja California UABC
Universidade Estadual Paulista (Unesp)
UABC
Univ Mogi das Cruzes
dc.contributor.author.fl_str_mv Marques, Victor H.
Moreira, Renata G.
Branco, Giovana S.
Honji, Renato M.
Rombenso, Artur N.
Viana, Maria Teresa
Mello, Paulo H. de [UNESP]
Mata-Sotres, Jose A.
Araujo, Bruno C.
dc.subject.por.fl_str_mv Fatty acids
Alternative lipids
SFA
MUFA
beta-Oxidation
topic Fatty acids
Alternative lipids
SFA
MUFA
beta-Oxidation
description This study aimed to investigate the influences of alternative lipid sources rich in saturated and monounsaturated fatty acids (SFA and MUFA) supplemented with long-chain polyunsaturated fatty acids (LC-PUFA) on production performance and lipid metabolism of Rachycentron canadum (cobia) juveniles. An 8-week feeding trial was carried out using four isoproteic and isolipidic diets as follows: FO-D (fish oil, as control diet), SFA-D (rich in SFA), MIX-D (same levels of SFA and MUFA), and MUFA-D (rich in MUFA). Experimental diets were supplemented with arachidonic acid (ARA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) (3, 5, and 10 g kg(1), respectively). The growth performance, fatty acid (FA) profile of liver and muscle, hepatocyte morphology, and gene expression related to the FA synthesis and oxidation on the liver were examined. In general, production performance was not impaired in fish-fed FO-free diets, supporting the hypothesis that alternative lipid sources could be used in cobia's aquafeed formulations when the LC-PUFA are adequately supplemented. High dietary SFA levels were disproportionally deposited the liver and muscle. Contrariwise MUFA was mainly deposited in the liver and muscle, reflecting the dietary inclusion levels. The main FA influencing this pattern were 12:0 and 18:1n-9. The expression of fatty acid synthase (fas) was up-regulated in the FO-D group compared to SFA-D and MIX-D groups. There were no differences in the relative expressions of carnitine palmitoyltransferase 1 (cpt-1 alpha) and lipase lipoprotein (lpl). The liver morphology results indicated that fish-fed SFA-D presented a smaller lipid vacuoles area than those fed other experimental diets. This study shows that SFA with shorter carbon chains such as 12:0 can be administered in cobia aquafeeds to stimulate these molecules' catabolism, providing energy for growth, and retaining LC-PUFAs in tissues, especially in the muscle, exhibiting a healthier fillet for consumers.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T15:07:11Z
2021-06-25T15:07:11Z
2021-08-30
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.aquaculture.2021.736843
Aquaculture. Amsterdam: Elsevier, v. 541, 12 p., 2021.
0044-8486
http://hdl.handle.net/11449/210396
10.1016/j.aquaculture.2021.736843
WOS:000657609900006
url http://dx.doi.org/10.1016/j.aquaculture.2021.736843
http://hdl.handle.net/11449/210396
identifier_str_mv Aquaculture. Amsterdam: Elsevier, v. 541, 12 p., 2021.
0044-8486
10.1016/j.aquaculture.2021.736843
WOS:000657609900006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Aquaculture
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 12
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129532695150592