Alterações celulares induzidas pelo estresse térmico em embriões bovinos

• Autor(a) principal: Lima, Rafaela Sanchez de [UNESP]
• Data de Publicação: 2013
• Outros Autores: Assumpção, Mayra Eelena Ortiz D'ávila de, Visintin, José Antonio, Lopes, Fabíola Freitas de Paula [UNESP]
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.11606/issn.2318-3659.v50i4p257-264; http://hdl.handle.net/11449/243795
• topic: Heat stress; Embryo; Bovine; Cellular changes; Estresse térmico; Embrião; Bovino; Alteraçõe celulares.

Adverse environmental conditions such as high temperature and humidity increase internal body temperature (hyperthermia) of lactating dairy cows resulting in heat stress and decreased pregnancy rates. Embryonic susceptibility to elevated temperature has been well characterized both in vivo and in vitro. Exposure of zygote and two cells stage bovine embryos to elevated temperature decreases embryonic development to the blastocyst stage. However, the bovine embryo becomes more resistant to the deleterious effects of heat stress as it proceeds in its development. The heat-induced reduction in embryonic developmental competence is due, at least in part, to the numerous cytoplasmic and nuclear changes induced by high temperature. In the embryo cytoplasm heat shock increases the number of swollen mitochondria, disrupts microtubules and microfilaments. In the nuclear compartment, elevate temperature induces DNA fragmentation characteristic of apoptosis. This form of cell death is a phenomenon regulated throughout the preimplantation embryonic development, since high temperatures do not trigger apoptosis in embryos of two or four cells. Heat-induced apoptosis in embryos > 16 cells can be seen as a quality control mechanism for removing damaged blastomeres, since block apoptosis in these embryos increase its susceptibility to heat shock. Furthermore, heat stress can also affect the redox status of the embryo inducing a consequent oxidative stress.