Mevalonate-derived quinonemethide triterpenoid from in vitro roots of Peritassa laevigata and their localization in root tissue by MALDI imaging
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/srep22627 http://hdl.handle.net/11449/165092 |
Resumo: | Biosynthetic investigation of quinonemethide triterpenoid 22 beta-hydroxy-maytenin (2) from in vitro root cultures of Peritassa laevigata (Celastraceae) was conducted using C-13-precursor. The mevalonate pathway in P. laevigata is responsible for the synthesis of the quinonemethide triterpenoid scaffold. Moreover, anatomical analysis of P. laevigata roots cultured in vitro and in situ showed the presence of 22 beta-hydroxy-maytenin (2) and maytenin (1) in the tissues from transverse or longitudinal sections with an intense orange color. MALDI-MS imaging confirmed the distribution of (2) and (1) in the more distal portions of the root cap, the outer cell layers, and near the vascular cylinder of P. laevigata in vitro roots suggesting a role in plant defense against infection by microorganisms as well as in the root exudation processes. |
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Mevalonate-derived quinonemethide triterpenoid from in vitro roots of Peritassa laevigata and their localization in root tissue by MALDI imagingBiosynthetic investigation of quinonemethide triterpenoid 22 beta-hydroxy-maytenin (2) from in vitro root cultures of Peritassa laevigata (Celastraceae) was conducted using C-13-precursor. The mevalonate pathway in P. laevigata is responsible for the synthesis of the quinonemethide triterpenoid scaffold. Moreover, anatomical analysis of P. laevigata roots cultured in vitro and in situ showed the presence of 22 beta-hydroxy-maytenin (2) and maytenin (1) in the tissues from transverse or longitudinal sections with an intense orange color. MALDI-MS imaging confirmed the distribution of (2) and (1) in the more distal portions of the root cap, the outer cell layers, and near the vascular cylinder of P. laevigata in vitro roots suggesting a role in plant defense against infection by microorganisms as well as in the root exudation processes.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Ribeirao Preto, Unidade Biotecnol, Av Costabile Romano 2201, BR-14096900 Ribeirao Preto, SP, BrazilUniv Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, NPPNS, BR-14040903 Ribeirao Preto, SP, BrazilUniv Fed Mato Grosso do Sul, Lab Prod Nat & Espectrometria Massas LAPNEM, BR-79070900 Campo Grande, MS, BrazilUniv Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Lab Bot, BR-14040903 Ribeirao Preto, SP, BrazilUniv Estadual Paulista, Inst Quim, BR-14801970 Araraquara, SP, BrazilUniv Estadual Paulista, Inst Quim, BR-14801970 Araraquara, SP, BrazilFAPESP: 2009/54098-6FAPESP: 2010/18132-2FAPESP: 2012/18031-7FAPESP: 2013/07349-9FAPESP: 2014/50265-3FAPESP: 2015/26066-3Nature Publishing GroupUniv Ribeirao PretoUniversidade de São Paulo (USP)Universidade Federal de Mato Grosso do Sul (UFMS)Universidade Estadual Paulista (Unesp)Pina, Edieidia S.Silva, Denise B.Teixeira, Simone P.Coppede, Juliana S.Furlan, Maysa [UNESP]Franca, Suzelei C.Lopes, Norberto P.Pereira, Ana Maria S.Lopes, Adriana A.2018-11-27T10:34:22Z2018-11-27T10:34:22Z2016-03-04info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttp://dx.doi.org/10.1038/srep22627Scientific Reports. London: Nature Publishing Group, v. 6, 7 p., 2016.2045-2322http://hdl.handle.net/11449/16509210.1038/srep22627WOS:000371523200001WOS000371523200001.pdf1308042794786872Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reports1,533info:eu-repo/semantics/openAccess2023-11-21T06:13:55Zoai:repositorio.unesp.br:11449/165092Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-21T06:13:55Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mevalonate-derived quinonemethide triterpenoid from in vitro roots of Peritassa laevigata and their localization in root tissue by MALDI imaging |
title |
Mevalonate-derived quinonemethide triterpenoid from in vitro roots of Peritassa laevigata and their localization in root tissue by MALDI imaging |
spellingShingle |
Mevalonate-derived quinonemethide triterpenoid from in vitro roots of Peritassa laevigata and their localization in root tissue by MALDI imaging Pina, Edieidia S. |
title_short |
Mevalonate-derived quinonemethide triterpenoid from in vitro roots of Peritassa laevigata and their localization in root tissue by MALDI imaging |
title_full |
Mevalonate-derived quinonemethide triterpenoid from in vitro roots of Peritassa laevigata and their localization in root tissue by MALDI imaging |
title_fullStr |
Mevalonate-derived quinonemethide triterpenoid from in vitro roots of Peritassa laevigata and their localization in root tissue by MALDI imaging |
title_full_unstemmed |
Mevalonate-derived quinonemethide triterpenoid from in vitro roots of Peritassa laevigata and their localization in root tissue by MALDI imaging |
title_sort |
Mevalonate-derived quinonemethide triterpenoid from in vitro roots of Peritassa laevigata and their localization in root tissue by MALDI imaging |
author |
Pina, Edieidia S. |
author_facet |
Pina, Edieidia S. Silva, Denise B. Teixeira, Simone P. Coppede, Juliana S. Furlan, Maysa [UNESP] Franca, Suzelei C. Lopes, Norberto P. Pereira, Ana Maria S. Lopes, Adriana A. |
author_role |
author |
author2 |
Silva, Denise B. Teixeira, Simone P. Coppede, Juliana S. Furlan, Maysa [UNESP] Franca, Suzelei C. Lopes, Norberto P. Pereira, Ana Maria S. Lopes, Adriana A. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Ribeirao Preto Universidade de São Paulo (USP) Universidade Federal de Mato Grosso do Sul (UFMS) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Pina, Edieidia S. Silva, Denise B. Teixeira, Simone P. Coppede, Juliana S. Furlan, Maysa [UNESP] Franca, Suzelei C. Lopes, Norberto P. Pereira, Ana Maria S. Lopes, Adriana A. |
description |
Biosynthetic investigation of quinonemethide triterpenoid 22 beta-hydroxy-maytenin (2) from in vitro root cultures of Peritassa laevigata (Celastraceae) was conducted using C-13-precursor. The mevalonate pathway in P. laevigata is responsible for the synthesis of the quinonemethide triterpenoid scaffold. Moreover, anatomical analysis of P. laevigata roots cultured in vitro and in situ showed the presence of 22 beta-hydroxy-maytenin (2) and maytenin (1) in the tissues from transverse or longitudinal sections with an intense orange color. MALDI-MS imaging confirmed the distribution of (2) and (1) in the more distal portions of the root cap, the outer cell layers, and near the vascular cylinder of P. laevigata in vitro roots suggesting a role in plant defense against infection by microorganisms as well as in the root exudation processes. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03-04 2018-11-27T10:34:22Z 2018-11-27T10:34:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/srep22627 Scientific Reports. London: Nature Publishing Group, v. 6, 7 p., 2016. 2045-2322 http://hdl.handle.net/11449/165092 10.1038/srep22627 WOS:000371523200001 WOS000371523200001.pdf 1308042794786872 |
url |
http://dx.doi.org/10.1038/srep22627 http://hdl.handle.net/11449/165092 |
identifier_str_mv |
Scientific Reports. London: Nature Publishing Group, v. 6, 7 p., 2016. 2045-2322 10.1038/srep22627 WOS:000371523200001 WOS000371523200001.pdf 1308042794786872 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports 1,533 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
7 application/pdf |
dc.publisher.none.fl_str_mv |
Nature Publishing Group |
publisher.none.fl_str_mv |
Nature Publishing Group |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1799965020440756224 |