Sporothrix pathogenic clade: Molecular analysis of animal and human clinical isolates
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/mmy/myac096 http://hdl.handle.net/11449/249023 |
Resumo: | Sporotrichosis is a subcutaneous mycosis that affects animals and humans. Varying in severity, occurrences range from local lesions to systemic involvement. It is caused by thermodimorphic and saprobic fungi from the Sporothrix pathogenic clade. This study aimed to identify the species and the sexual idiomorph distribution patterns responsible for diagnosed cases of sporotrichosis in São José do Rio Preto, Brazil. We included 188 isolates of Sporothrix sp. from feline lesions and 27 of human origin, which underwent molecular identification and genotyping for mating-type MAT1-1 and MAT1-2. The results showed that Sporothrix brasiliensis is the prevalent species in feline sporotrichosis outbreaks with the overwhelming presence of a single mating-type, MAT1-2 (P <.0001), suggesting a prevalently clonal form of spread. Morphological analyses did not discriminate among cryptic species in the genus Sporothrix, and molecular identification was essential for the correct identification of the species responsible for the observed cases of sporotrichosis. Distribution analyses of MAT1-2 isolates support the hypothesis of unidirectional migration from the current epidemics in São Paulo and Rio de Janeiro to the municipality of São José do Rio Preto. |
id |
UNSP_ef7b2431d698c9d988a16ea1d6581235 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/249023 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Sporothrix pathogenic clade: Molecular analysis of animal and human clinical isolatesMAT1-2mating-typespecies-specific PCRSporothrix brasiliensissporotrichosisSporotrichosis is a subcutaneous mycosis that affects animals and humans. Varying in severity, occurrences range from local lesions to systemic involvement. It is caused by thermodimorphic and saprobic fungi from the Sporothrix pathogenic clade. This study aimed to identify the species and the sexual idiomorph distribution patterns responsible for diagnosed cases of sporotrichosis in São José do Rio Preto, Brazil. We included 188 isolates of Sporothrix sp. from feline lesions and 27 of human origin, which underwent molecular identification and genotyping for mating-type MAT1-1 and MAT1-2. The results showed that Sporothrix brasiliensis is the prevalent species in feline sporotrichosis outbreaks with the overwhelming presence of a single mating-type, MAT1-2 (P <.0001), suggesting a prevalently clonal form of spread. Morphological analyses did not discriminate among cryptic species in the genus Sporothrix, and molecular identification was essential for the correct identification of the species responsible for the observed cases of sporotrichosis. Distribution analyses of MAT1-2 isolates support the hypothesis of unidirectional migration from the current epidemics in São Paulo and Rio de Janeiro to the municipality of São José do Rio Preto.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Dermatological Infectious and Parasitic Diseases São José Do Rio Preto School of Medicine (FAMERP), São PauloInstitute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP), São PauloDepartment of Microbiology Immunology and Parasitology Laboratory of Emerging Fungal Pathogens Federal University of São Paulo, São PauloInstitute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP), São PauloCAPES: CAPESCNPq: CNPqFAPESP: FAPESPSão José Do Rio Preto School of Medicine (FAMERP)Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Ribeiro-Marques, Mariela DomicianoMaschio-Lima, Taiza [UNESP]Lemes, Thiago Henrique [UNESP]Siqueira, João Paulo ZenBrizzotti-Mazuchi, Natália SeronCaetano, Maicon Henrique [UNESP]Almeida, Bianca GottardoMozaner, Leticia Queiroz [UNESP]Monteiro, Ruan CamposCamargo, Zoilo PiresRodrigues, Anderson MessiasGottardo De Almeida, Margarete Teresa [UNESP]2023-07-29T14:00:16Z2023-07-29T14:00:16Z2023-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1093/mmy/myac096Medical Mycology, v. 61, n. 1, 2023.1460-27091369-3786http://hdl.handle.net/11449/24902310.1093/mmy/myac0962-s2.0-85145022687Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMedical Mycologyinfo:eu-repo/semantics/openAccess2023-07-29T14:00:16Zoai:repositorio.unesp.br:11449/249023Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T14:00:16Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Sporothrix pathogenic clade: Molecular analysis of animal and human clinical isolates |
title |
Sporothrix pathogenic clade: Molecular analysis of animal and human clinical isolates |
spellingShingle |
Sporothrix pathogenic clade: Molecular analysis of animal and human clinical isolates Ribeiro-Marques, Mariela Domiciano MAT1-2 mating-type species-specific PCR Sporothrix brasiliensis sporotrichosis |
title_short |
Sporothrix pathogenic clade: Molecular analysis of animal and human clinical isolates |
title_full |
Sporothrix pathogenic clade: Molecular analysis of animal and human clinical isolates |
title_fullStr |
Sporothrix pathogenic clade: Molecular analysis of animal and human clinical isolates |
title_full_unstemmed |
Sporothrix pathogenic clade: Molecular analysis of animal and human clinical isolates |
title_sort |
Sporothrix pathogenic clade: Molecular analysis of animal and human clinical isolates |
author |
Ribeiro-Marques, Mariela Domiciano |
author_facet |
Ribeiro-Marques, Mariela Domiciano Maschio-Lima, Taiza [UNESP] Lemes, Thiago Henrique [UNESP] Siqueira, João Paulo Zen Brizzotti-Mazuchi, Natália Seron Caetano, Maicon Henrique [UNESP] Almeida, Bianca Gottardo Mozaner, Leticia Queiroz [UNESP] Monteiro, Ruan Campos Camargo, Zoilo Pires Rodrigues, Anderson Messias Gottardo De Almeida, Margarete Teresa [UNESP] |
author_role |
author |
author2 |
Maschio-Lima, Taiza [UNESP] Lemes, Thiago Henrique [UNESP] Siqueira, João Paulo Zen Brizzotti-Mazuchi, Natália Seron Caetano, Maicon Henrique [UNESP] Almeida, Bianca Gottardo Mozaner, Leticia Queiroz [UNESP] Monteiro, Ruan Campos Camargo, Zoilo Pires Rodrigues, Anderson Messias Gottardo De Almeida, Margarete Teresa [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
São José Do Rio Preto School of Medicine (FAMERP) Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Ribeiro-Marques, Mariela Domiciano Maschio-Lima, Taiza [UNESP] Lemes, Thiago Henrique [UNESP] Siqueira, João Paulo Zen Brizzotti-Mazuchi, Natália Seron Caetano, Maicon Henrique [UNESP] Almeida, Bianca Gottardo Mozaner, Leticia Queiroz [UNESP] Monteiro, Ruan Campos Camargo, Zoilo Pires Rodrigues, Anderson Messias Gottardo De Almeida, Margarete Teresa [UNESP] |
dc.subject.por.fl_str_mv |
MAT1-2 mating-type species-specific PCR Sporothrix brasiliensis sporotrichosis |
topic |
MAT1-2 mating-type species-specific PCR Sporothrix brasiliensis sporotrichosis |
description |
Sporotrichosis is a subcutaneous mycosis that affects animals and humans. Varying in severity, occurrences range from local lesions to systemic involvement. It is caused by thermodimorphic and saprobic fungi from the Sporothrix pathogenic clade. This study aimed to identify the species and the sexual idiomorph distribution patterns responsible for diagnosed cases of sporotrichosis in São José do Rio Preto, Brazil. We included 188 isolates of Sporothrix sp. from feline lesions and 27 of human origin, which underwent molecular identification and genotyping for mating-type MAT1-1 and MAT1-2. The results showed that Sporothrix brasiliensis is the prevalent species in feline sporotrichosis outbreaks with the overwhelming presence of a single mating-type, MAT1-2 (P <.0001), suggesting a prevalently clonal form of spread. Morphological analyses did not discriminate among cryptic species in the genus Sporothrix, and molecular identification was essential for the correct identification of the species responsible for the observed cases of sporotrichosis. Distribution analyses of MAT1-2 isolates support the hypothesis of unidirectional migration from the current epidemics in São Paulo and Rio de Janeiro to the municipality of São José do Rio Preto. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T14:00:16Z 2023-07-29T14:00:16Z 2023-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/mmy/myac096 Medical Mycology, v. 61, n. 1, 2023. 1460-2709 1369-3786 http://hdl.handle.net/11449/249023 10.1093/mmy/myac096 2-s2.0-85145022687 |
url |
http://dx.doi.org/10.1093/mmy/myac096 http://hdl.handle.net/11449/249023 |
identifier_str_mv |
Medical Mycology, v. 61, n. 1, 2023. 1460-2709 1369-3786 10.1093/mmy/myac096 2-s2.0-85145022687 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Medical Mycology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964547660906496 |