Fsh Stimulates Spermatogonial Proliferation and Differentiation in Zebrafish via Igf3
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1210/en.2015-1157 http://hdl.handle.net/11449/160877 |
Resumo: | Growth factors modulate germ line stem cell self-renewal and differentiation behavior. We investigate the effects of Igf3, a fish-specific member of the igf family. Fsh increased in a steroid-independent manner the number and mitotic index of single type A undifferentiated spermatogonia and of clones of type A differentiating spermatogonia in adult zebrafish testis. All 4 igf gene family members in zebrafish are expressed in the testis but in tissue culture only igf3 transcript levels increased in response to recombinant zebrafish Fsh. This occurred in a cAMP/protein kinase A-dependent manner, in line with the results of studies on the igf3 gene promoter. Igf3 protein was detected in Sertoli cells. Recombinant zebrafish Igf3 increased the mitotic index of type A undifferentiated and type A differentiating spermatogonia and up-regulated the expression of genes related to spermatogonial differentiation and entry into meiosis, but Igf3 did not modulate testicular androgen release. An Igf receptor inhibitor blocked these effects of Igf3. Importantly, the Igf receptor inhibitor also blocked Fsh-induced spermatogonial proliferation. We conclude that Fsh stimulated Sertoli cell production of Igf3, which promoted via Igf receptor signaling spermatogonial proliferation and differentiation and their entry into meiosis. Because previous work showed that Fsh also released spermatogonia from an inhibitory signal by down-regulating anti Mullerian hormone and by stimulating androgen production, we can now present a model, in which Fsh orchestrates the activity of stimulatory (Igf3, androgens) and inhibitory (anti-Mullerian hormone) signals to promote spermatogenesis. |
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Fsh Stimulates Spermatogonial Proliferation and Differentiation in Zebrafish via Igf3Growth factors modulate germ line stem cell self-renewal and differentiation behavior. We investigate the effects of Igf3, a fish-specific member of the igf family. Fsh increased in a steroid-independent manner the number and mitotic index of single type A undifferentiated spermatogonia and of clones of type A differentiating spermatogonia in adult zebrafish testis. All 4 igf gene family members in zebrafish are expressed in the testis but in tissue culture only igf3 transcript levels increased in response to recombinant zebrafish Fsh. This occurred in a cAMP/protein kinase A-dependent manner, in line with the results of studies on the igf3 gene promoter. Igf3 protein was detected in Sertoli cells. Recombinant zebrafish Igf3 increased the mitotic index of type A undifferentiated and type A differentiating spermatogonia and up-regulated the expression of genes related to spermatogonial differentiation and entry into meiosis, but Igf3 did not modulate testicular androgen release. An Igf receptor inhibitor blocked these effects of Igf3. Importantly, the Igf receptor inhibitor also blocked Fsh-induced spermatogonial proliferation. We conclude that Fsh stimulated Sertoli cell production of Igf3, which promoted via Igf receptor signaling spermatogonial proliferation and differentiation and their entry into meiosis. Because previous work showed that Fsh also released spermatogonia from an inhibitory signal by down-regulating anti Mullerian hormone and by stimulating androgen production, we can now present a model, in which Fsh orchestrates the activity of stimulatory (Igf3, androgens) and inhibitory (anti-Mullerian hormone) signals to promote spermatogenesis.Utrecht UniversityEuropean UnionNorwegian Research CouncilBrazilian funding agency Coordination for the Improvement of Higher Level PersonnelFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Brazilian funding agency National Council for Scientific and Technological DevelopmentSao Paulo State Univ, Inst Biosci, Dept Morphol, BR-18618970 Botucatu, SP, BrazilUniv Utrecht, Fac Sci, Dept Biol, Div Dev Biol,Reprod Biol Grp, NL-3584 CH Utrecht, NetherlandsUniv Fed Minas Gerais, Inst Biol Sci, Dept Morphol, Lab Cellular Biol, BR-31270901 Belo Horizonte, MG, BrazilSao Paulo State Univ, Inst Biosci, Dept Morphol, BR-18618970 Botucatu, SP, BrazilEuropean Union: LIFECYCLE FP7-222719Norwegian Research Council: 159045Endocrine SocUniversidade Estadual Paulista (Unesp)Univ UtrechtUniversidade Federal de Minas Gerais (UFMG)Nobrega, Rafael Henrique [UNESP]Vidal de Souza Morais, Roberto DaltroCrespo, DiegoWaal, Paul P. deFranca, Luiz Renato deSchulz, Rudiger W.Bogerd, Jan2018-11-26T16:17:06Z2018-11-26T16:17:06Z2015-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article3804-3817application/pdfhttp://dx.doi.org/10.1210/en.2015-1157Endocrinology. Washington: Endocrine Soc, v. 156, n. 10, p. 3804-3817, 2015.0013-7227http://hdl.handle.net/11449/16087710.1210/en.2015-1157WOS:000362235300044WOS000362235300044.pdf05157085852539850000-0001-9796-5076Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEndocrinology1,878info:eu-repo/semantics/openAccess2023-10-27T06:09:01Zoai:repositorio.unesp.br:11449/160877Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-27T06:09:01Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Fsh Stimulates Spermatogonial Proliferation and Differentiation in Zebrafish via Igf3 |
title |
Fsh Stimulates Spermatogonial Proliferation and Differentiation in Zebrafish via Igf3 |
spellingShingle |
Fsh Stimulates Spermatogonial Proliferation and Differentiation in Zebrafish via Igf3 Nobrega, Rafael Henrique [UNESP] |
title_short |
Fsh Stimulates Spermatogonial Proliferation and Differentiation in Zebrafish via Igf3 |
title_full |
Fsh Stimulates Spermatogonial Proliferation and Differentiation in Zebrafish via Igf3 |
title_fullStr |
Fsh Stimulates Spermatogonial Proliferation and Differentiation in Zebrafish via Igf3 |
title_full_unstemmed |
Fsh Stimulates Spermatogonial Proliferation and Differentiation in Zebrafish via Igf3 |
title_sort |
Fsh Stimulates Spermatogonial Proliferation and Differentiation in Zebrafish via Igf3 |
author |
Nobrega, Rafael Henrique [UNESP] |
author_facet |
Nobrega, Rafael Henrique [UNESP] Vidal de Souza Morais, Roberto Daltro Crespo, Diego Waal, Paul P. de Franca, Luiz Renato de Schulz, Rudiger W. Bogerd, Jan |
author_role |
author |
author2 |
Vidal de Souza Morais, Roberto Daltro Crespo, Diego Waal, Paul P. de Franca, Luiz Renato de Schulz, Rudiger W. Bogerd, Jan |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Univ Utrecht Universidade Federal de Minas Gerais (UFMG) |
dc.contributor.author.fl_str_mv |
Nobrega, Rafael Henrique [UNESP] Vidal de Souza Morais, Roberto Daltro Crespo, Diego Waal, Paul P. de Franca, Luiz Renato de Schulz, Rudiger W. Bogerd, Jan |
description |
Growth factors modulate germ line stem cell self-renewal and differentiation behavior. We investigate the effects of Igf3, a fish-specific member of the igf family. Fsh increased in a steroid-independent manner the number and mitotic index of single type A undifferentiated spermatogonia and of clones of type A differentiating spermatogonia in adult zebrafish testis. All 4 igf gene family members in zebrafish are expressed in the testis but in tissue culture only igf3 transcript levels increased in response to recombinant zebrafish Fsh. This occurred in a cAMP/protein kinase A-dependent manner, in line with the results of studies on the igf3 gene promoter. Igf3 protein was detected in Sertoli cells. Recombinant zebrafish Igf3 increased the mitotic index of type A undifferentiated and type A differentiating spermatogonia and up-regulated the expression of genes related to spermatogonial differentiation and entry into meiosis, but Igf3 did not modulate testicular androgen release. An Igf receptor inhibitor blocked these effects of Igf3. Importantly, the Igf receptor inhibitor also blocked Fsh-induced spermatogonial proliferation. We conclude that Fsh stimulated Sertoli cell production of Igf3, which promoted via Igf receptor signaling spermatogonial proliferation and differentiation and their entry into meiosis. Because previous work showed that Fsh also released spermatogonia from an inhibitory signal by down-regulating anti Mullerian hormone and by stimulating androgen production, we can now present a model, in which Fsh orchestrates the activity of stimulatory (Igf3, androgens) and inhibitory (anti-Mullerian hormone) signals to promote spermatogenesis. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-01 2018-11-26T16:17:06Z 2018-11-26T16:17:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1210/en.2015-1157 Endocrinology. Washington: Endocrine Soc, v. 156, n. 10, p. 3804-3817, 2015. 0013-7227 http://hdl.handle.net/11449/160877 10.1210/en.2015-1157 WOS:000362235300044 WOS000362235300044.pdf 0515708585253985 0000-0001-9796-5076 |
url |
http://dx.doi.org/10.1210/en.2015-1157 http://hdl.handle.net/11449/160877 |
identifier_str_mv |
Endocrinology. Washington: Endocrine Soc, v. 156, n. 10, p. 3804-3817, 2015. 0013-7227 10.1210/en.2015-1157 WOS:000362235300044 WOS000362235300044.pdf 0515708585253985 0000-0001-9796-5076 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Endocrinology 1,878 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
3804-3817 application/pdf |
dc.publisher.none.fl_str_mv |
Endocrine Soc |
publisher.none.fl_str_mv |
Endocrine Soc |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964720484057088 |