DNA Barcode na identificação de Peixes de Bico explorados comercialmente: uma abordagem forense

• Autor(a) principal: Rodrigues Junior, Carlos Egberto [UNESP]
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/152086
• topic: Peixe de bico; Xiphias gladius; Billfish; White marlin; Marlim branco; Espadarte

The billfish are oceanic pelagic species often caught by the tuna fleet and with great interest of sport fishing. This group of fish has specific legislation prohibiting the marketing and export of two species, Kajuikia albida e Makaira nigricans, in Brazil. DNA barcode is a universal system of molecular identification based on a sequence of mitochondrial DNA cytochrome oxidase subunit I (COI) of about 650 base pairs, serving as an identifying genomic mark in each species. This system has been used successfully to identify mischaracterized samples illegally marketed, like sample materials from sharks and other endangered species of organisms. The Barcode DNA technique was used to identify billfish marketed in the second largest fishing warehouse in Latin America, the CEAGESP-SP and the international airport of São Paulo, Brazil. 302 samples of billfish were collected during three inspection actions carried out by IBAMA, the executing agency of the national environmental policy. After DNA sequencing procedures it was possible to identify 72 samples (91.14%) by CEAGESP at the level of species, being 21 (29.17%) identified as Xiphias gladius, 44 (61.1%) as Istiophorus platypterus and 7 (8.33%) as Kajikia albida, this latter species determined as a market prohibited in Brazil and considered vulnerable in the list of endangered species of the IUCN and in the official list of species of endangered Fauna - Fish and Aquatic Invertebrates. At the International Airport of Guarulhos it was possible to identify 223 samples (100%), all identified as Xiphias gladius as declared by the exporter and indicated by its NCM (Mercosur Common Nomenclature). Molecular analysis has proven to be an excellent tool to identify billfish in Brazil in different centers such as Guarulhos International Airport and CEAGESP, SP. The identification of the white marlin being illegally traded in CEAGESP demonstrates the ineffectiveness of the current monitoring techniques used and emphasize the need for adopting better public policies for the conservation of the species.