Morphological, immunohistochemical and molecular study of renal lesions in canine visceral leishmaniasis (CVL)

Detalhes bibliográficos
Autor(a) principal: Ferrari, Heitor Flavio
Data de Publicação: 2010
Outros Autores: Carreira, Vinícius Soares, Moreira, Márcio Anontio Batistela, Matta, Vania Ribeiro da, Haga, Claudio Hiroyassu, Luvizotto, Maria Cecília Rui [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.fmvz.unesp.br/rvz/index.php/rvz/article/view/304
http://hdl.handle.net/11449/133094
Resumo: Canine visceral leishmaniasis (CVL) is an anthropozoonosis characterized by a clinically chronic progressive disease. Non lymphoid organs are also affected, especially the kidneys. Dogs with leishmaniasis usually die with renal failure despite treatment. Haematoxylin-eosin (HE) staining in kidney tissue sections has low sensitivity for parasite identification. Immunohistochemistry (IHC) and polymerase chain reaction (PCR) are efficient methods for Leishmania sp. antigen and DNA detection in cases of low parasite burden. The present study aims to identify renal lesions of CVL and correlate them with microscopic findings determined by histochemistry, IHC and PCR. Both IHC and PCR provided similar positivity for amastigote identification, 3/20 animals (15%), thus increasing detection of the parasite in renal tissues when compared with histopathologic examination. The lesion most commonly observed with visceral leishmaniasis-positive canine kidney tissue was membranoproliferative glomerulonephritis, followed by interstitial nephritis without correlation to the number of amastigotes.
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spelling Morphological, immunohistochemical and molecular study of renal lesions in canine visceral leishmaniasis (CVL)Studio morfológico, inmunoistoquímico y molecular en riñones de perros portadores de leishmaniosis visceralDogVisceral leishmaniasisKidneyImmunohistochemistryPolymerase chain reactionPerroLeishmaniosis visceralRiñonesInmunoistoquímicaReacción en Cadena por la Polimerase (PCR)Canine visceral leishmaniasis (CVL) is an anthropozoonosis characterized by a clinically chronic progressive disease. Non lymphoid organs are also affected, especially the kidneys. Dogs with leishmaniasis usually die with renal failure despite treatment. Haematoxylin-eosin (HE) staining in kidney tissue sections has low sensitivity for parasite identification. Immunohistochemistry (IHC) and polymerase chain reaction (PCR) are efficient methods for Leishmania sp. antigen and DNA detection in cases of low parasite burden. The present study aims to identify renal lesions of CVL and correlate them with microscopic findings determined by histochemistry, IHC and PCR. Both IHC and PCR provided similar positivity for amastigote identification, 3/20 animals (15%), thus increasing detection of the parasite in renal tissues when compared with histopathologic examination. The lesion most commonly observed with visceral leishmaniasis-positive canine kidney tissue was membranoproliferative glomerulonephritis, followed by interstitial nephritis without correlation to the number of amastigotes.La leishmaniosis visceral canina (CVL) es una antropozoonosis caracterizada por evolución crónica y progresiva. Órganos no pertenecientes al sistema linfático también son afectados, destacándose los riñones. Perros con leishmaniosis desarrolan lesión renal crónica, que evoluye al óbito, aún después del tratamiento. La identificación del parásito en la tejido renal, corado con hematoxilina y eosina (HE), presenta baja sensibilidad. Las técnicas de inmunoistoquímica (IHC) y de Reacción en Cadena por la Polimerase (PCR) son eficientes en la detección del antígeno y del DNA de la Leishmania sp en los casos de bajo parasitismo. El presente trabajo tuvo como objetivos identificar las alteraciones renales decurrentes de la CVL y asociar con lesiones histológicas observadas en las coloraciones de histoquímica, IHC y PCR. El análisis de la IHC y PCR revelaron positividad semejante en la detección amastigotas, 3/20 animales (15%), lo que muestra el parásito en el tejido renal, cuando comparado con el examen histopatológico HE. En los casos de leishmaniosis visceral con exhibición en de amastigotas en el tejido renal, las lesiones microscópicas predominantes fueron glomerulonefritis membranoproliferativa y nefritis intersticial, no correlacionada con número de amastigotas.Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Clínica Cirurgia e Reprodução Animal, Aracatuba, Rua Clóvis Pestana, 793, Jardim Dona Amelia, CEP 16050-680, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Clínica Cirurgia e Reprodução Animal, Aracatuba, Rua Clóvis Pestana, 793, Jardim Dona Amelia, CEP 16050-680, SP, BrasilFaculdade de Medicina Veterinária de AndradinaMichigan State UniversityFaculdade Anhembi MorumbUniversidade de São Paulo(USP)Universidade Estadual Paulista (Unesp)Ferrari, Heitor FlavioCarreira, Vinícius SoaresMoreira, Márcio Anontio BatistelaMatta, Vania Ribeiro daHaga, Claudio HiroyassuLuvizotto, Maria Cecília Rui [UNESP]2016-01-28T16:53:36Z2016-01-28T16:53:36Z2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article123-131application/pdfhttp://www.fmvz.unesp.br/rvz/index.php/rvz/article/view/304Veterinária e Zootecnia, v. 17, n. 1, p. 123-131, 2010.0102-5716http://hdl.handle.net/11449/133094ISSN0102-5716-2010-17-01-123-131.pdf28504531837243614584674909952477Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinária e Zootecniainfo:eu-repo/semantics/openAccess2024-09-04T18:03:59Zoai:repositorio.unesp.br:11449/133094Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T18:03:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Morphological, immunohistochemical and molecular study of renal lesions in canine visceral leishmaniasis (CVL)
Studio morfológico, inmunoistoquímico y molecular en riñones de perros portadores de leishmaniosis visceral
title Morphological, immunohistochemical and molecular study of renal lesions in canine visceral leishmaniasis (CVL)
spellingShingle Morphological, immunohistochemical and molecular study of renal lesions in canine visceral leishmaniasis (CVL)
Ferrari, Heitor Flavio
Dog
Visceral leishmaniasis
Kidney
Immunohistochemistry
Polymerase chain reaction
Perro
Leishmaniosis visceral
Riñones
Inmunoistoquímica
Reacción en Cadena por la Polimerase (PCR)
title_short Morphological, immunohistochemical and molecular study of renal lesions in canine visceral leishmaniasis (CVL)
title_full Morphological, immunohistochemical and molecular study of renal lesions in canine visceral leishmaniasis (CVL)
title_fullStr Morphological, immunohistochemical and molecular study of renal lesions in canine visceral leishmaniasis (CVL)
title_full_unstemmed Morphological, immunohistochemical and molecular study of renal lesions in canine visceral leishmaniasis (CVL)
title_sort Morphological, immunohistochemical and molecular study of renal lesions in canine visceral leishmaniasis (CVL)
author Ferrari, Heitor Flavio
author_facet Ferrari, Heitor Flavio
Carreira, Vinícius Soares
Moreira, Márcio Anontio Batistela
Matta, Vania Ribeiro da
Haga, Claudio Hiroyassu
Luvizotto, Maria Cecília Rui [UNESP]
author_role author
author2 Carreira, Vinícius Soares
Moreira, Márcio Anontio Batistela
Matta, Vania Ribeiro da
Haga, Claudio Hiroyassu
Luvizotto, Maria Cecília Rui [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Faculdade de Medicina Veterinária de Andradina
Michigan State University
Faculdade Anhembi Morumb
Universidade de São Paulo(USP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Ferrari, Heitor Flavio
Carreira, Vinícius Soares
Moreira, Márcio Anontio Batistela
Matta, Vania Ribeiro da
Haga, Claudio Hiroyassu
Luvizotto, Maria Cecília Rui [UNESP]
dc.subject.por.fl_str_mv Dog
Visceral leishmaniasis
Kidney
Immunohistochemistry
Polymerase chain reaction
Perro
Leishmaniosis visceral
Riñones
Inmunoistoquímica
Reacción en Cadena por la Polimerase (PCR)
topic Dog
Visceral leishmaniasis
Kidney
Immunohistochemistry
Polymerase chain reaction
Perro
Leishmaniosis visceral
Riñones
Inmunoistoquímica
Reacción en Cadena por la Polimerase (PCR)
description Canine visceral leishmaniasis (CVL) is an anthropozoonosis characterized by a clinically chronic progressive disease. Non lymphoid organs are also affected, especially the kidneys. Dogs with leishmaniasis usually die with renal failure despite treatment. Haematoxylin-eosin (HE) staining in kidney tissue sections has low sensitivity for parasite identification. Immunohistochemistry (IHC) and polymerase chain reaction (PCR) are efficient methods for Leishmania sp. antigen and DNA detection in cases of low parasite burden. The present study aims to identify renal lesions of CVL and correlate them with microscopic findings determined by histochemistry, IHC and PCR. Both IHC and PCR provided similar positivity for amastigote identification, 3/20 animals (15%), thus increasing detection of the parasite in renal tissues when compared with histopathologic examination. The lesion most commonly observed with visceral leishmaniasis-positive canine kidney tissue was membranoproliferative glomerulonephritis, followed by interstitial nephritis without correlation to the number of amastigotes.
publishDate 2010
dc.date.none.fl_str_mv 2010
2016-01-28T16:53:36Z
2016-01-28T16:53:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.fmvz.unesp.br/rvz/index.php/rvz/article/view/304
Veterinária e Zootecnia, v. 17, n. 1, p. 123-131, 2010.
0102-5716
http://hdl.handle.net/11449/133094
ISSN0102-5716-2010-17-01-123-131.pdf
2850453183724361
4584674909952477
url http://www.fmvz.unesp.br/rvz/index.php/rvz/article/view/304
http://hdl.handle.net/11449/133094
identifier_str_mv Veterinária e Zootecnia, v. 17, n. 1, p. 123-131, 2010.
0102-5716
ISSN0102-5716-2010-17-01-123-131.pdf
2850453183724361
4584674909952477
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Veterinária e Zootecnia
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 123-131
application/pdf
dc.source.none.fl_str_mv Currículo Lattes
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1810021398814392320

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