Simple and rapid hplc-uv methods for gabapentin quantification in human plasma and urine: Applicability in pharmacokinetics and drug monitoring
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4322/2179-443X.0717 http://hdl.handle.net/11449/207701 |
Resumo: | Aim: We aimed to develop methods to determine gabapentin (GAB) in biological samples using high-performance liquid chromatography (HPLC) with application in pharmacokinetics and therapeutic drug monitoring. Methods: Simple, rapid and efficient HPLC-UV methods to quantify GAB in human plasma and urine were developed and validated for clinical analysis of GAB. The 1-fluoro-2,4-dinitrobenzene (FDNB) was used as derivatization agent. For plasma samples, protein precipitation using acetonitrile was performed, before the derivatization reaction. Urine samples were cleaned-up by liquid-liquid extraction with dichloromethane:n-butanol (1:1, v/v) after derivatized. Amlodipine besilate was used as internal standard (IS). Results: Gabapentin and IS were resolved on LiChrospher® C18 RP column and a mixture of 50 mM sodium phosphate buffer (pH 3.9):methanol (27:73, v/v) as mobile phase, at 1.2 mL/min. The methods used small sample volumes, 100 and 50 µL of plasma and urine, respectively. Linearity was obtained in the interval of 0.2-14 µg/mL in plasma and 2-120 µg/mL in urine. Both methods showed to be selective, without carry-over effect, precise, accurate and stable in different conditions. GAB plasma concentration in patients receiving 600 to 3600 mg/day of GAB ranged between 0.40 to 11.94 µg/mL at steady-state. Conclusions: The methods described in this study were simple, rapid and fulfill all validation requirements. They were easily and successfully applied for population pharmacokinetics and therapeutic drug monitoring of GAB in patients with chronic pain. |
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Simple and rapid hplc-uv methods for gabapentin quantification in human plasma and urine: Applicability in pharmacokinetics and drug monitoringGabapentinHPLCPlasmaUrineAim: We aimed to develop methods to determine gabapentin (GAB) in biological samples using high-performance liquid chromatography (HPLC) with application in pharmacokinetics and therapeutic drug monitoring. Methods: Simple, rapid and efficient HPLC-UV methods to quantify GAB in human plasma and urine were developed and validated for clinical analysis of GAB. The 1-fluoro-2,4-dinitrobenzene (FDNB) was used as derivatization agent. For plasma samples, protein precipitation using acetonitrile was performed, before the derivatization reaction. Urine samples were cleaned-up by liquid-liquid extraction with dichloromethane:n-butanol (1:1, v/v) after derivatized. Amlodipine besilate was used as internal standard (IS). Results: Gabapentin and IS were resolved on LiChrospher® C18 RP column and a mixture of 50 mM sodium phosphate buffer (pH 3.9):methanol (27:73, v/v) as mobile phase, at 1.2 mL/min. The methods used small sample volumes, 100 and 50 µL of plasma and urine, respectively. Linearity was obtained in the interval of 0.2-14 µg/mL in plasma and 2-120 µg/mL in urine. Both methods showed to be selective, without carry-over effect, precise, accurate and stable in different conditions. GAB plasma concentration in patients receiving 600 to 3600 mg/day of GAB ranged between 0.40 to 11.94 µg/mL at steady-state. Conclusions: The methods described in this study were simple, rapid and fulfill all validation requirements. They were easily and successfully applied for population pharmacokinetics and therapeutic drug monitoring of GAB in patients with chronic pain.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Fármacos e Medicamentos Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)Departamento de Análises Clínicas Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo (USP)Departamento de Fármacos e Medicamentos Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)CAPES: 001FAPESP: 2015/25728-2Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Yamamoto, Priscila Akemi [UNESP]Benzi, Jhohann Richard de Limade Moraes, Natália Valadares [UNESP]2021-06-25T10:59:31Z2021-06-25T10:59:31Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.4322/2179-443X.0717Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 42.2179-443X1808-4532http://hdl.handle.net/11449/20770110.4322/2179-443X.07172-s2.0-85105390895Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista de Ciencias Farmaceuticas Basica e Aplicadainfo:eu-repo/semantics/openAccess2024-06-24T13:45:18Zoai:repositorio.unesp.br:11449/207701Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:37:55.137892Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Simple and rapid hplc-uv methods for gabapentin quantification in human plasma and urine: Applicability in pharmacokinetics and drug monitoring |
title |
Simple and rapid hplc-uv methods for gabapentin quantification in human plasma and urine: Applicability in pharmacokinetics and drug monitoring |
spellingShingle |
Simple and rapid hplc-uv methods for gabapentin quantification in human plasma and urine: Applicability in pharmacokinetics and drug monitoring Yamamoto, Priscila Akemi [UNESP] Gabapentin HPLC Plasma Urine |
title_short |
Simple and rapid hplc-uv methods for gabapentin quantification in human plasma and urine: Applicability in pharmacokinetics and drug monitoring |
title_full |
Simple and rapid hplc-uv methods for gabapentin quantification in human plasma and urine: Applicability in pharmacokinetics and drug monitoring |
title_fullStr |
Simple and rapid hplc-uv methods for gabapentin quantification in human plasma and urine: Applicability in pharmacokinetics and drug monitoring |
title_full_unstemmed |
Simple and rapid hplc-uv methods for gabapentin quantification in human plasma and urine: Applicability in pharmacokinetics and drug monitoring |
title_sort |
Simple and rapid hplc-uv methods for gabapentin quantification in human plasma and urine: Applicability in pharmacokinetics and drug monitoring |
author |
Yamamoto, Priscila Akemi [UNESP] |
author_facet |
Yamamoto, Priscila Akemi [UNESP] Benzi, Jhohann Richard de Lima de Moraes, Natália Valadares [UNESP] |
author_role |
author |
author2 |
Benzi, Jhohann Richard de Lima de Moraes, Natália Valadares [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Yamamoto, Priscila Akemi [UNESP] Benzi, Jhohann Richard de Lima de Moraes, Natália Valadares [UNESP] |
dc.subject.por.fl_str_mv |
Gabapentin HPLC Plasma Urine |
topic |
Gabapentin HPLC Plasma Urine |
description |
Aim: We aimed to develop methods to determine gabapentin (GAB) in biological samples using high-performance liquid chromatography (HPLC) with application in pharmacokinetics and therapeutic drug monitoring. Methods: Simple, rapid and efficient HPLC-UV methods to quantify GAB in human plasma and urine were developed and validated for clinical analysis of GAB. The 1-fluoro-2,4-dinitrobenzene (FDNB) was used as derivatization agent. For plasma samples, protein precipitation using acetonitrile was performed, before the derivatization reaction. Urine samples were cleaned-up by liquid-liquid extraction with dichloromethane:n-butanol (1:1, v/v) after derivatized. Amlodipine besilate was used as internal standard (IS). Results: Gabapentin and IS were resolved on LiChrospher® C18 RP column and a mixture of 50 mM sodium phosphate buffer (pH 3.9):methanol (27:73, v/v) as mobile phase, at 1.2 mL/min. The methods used small sample volumes, 100 and 50 µL of plasma and urine, respectively. Linearity was obtained in the interval of 0.2-14 µg/mL in plasma and 2-120 µg/mL in urine. Both methods showed to be selective, without carry-over effect, precise, accurate and stable in different conditions. GAB plasma concentration in patients receiving 600 to 3600 mg/day of GAB ranged between 0.40 to 11.94 µg/mL at steady-state. Conclusions: The methods described in this study were simple, rapid and fulfill all validation requirements. They were easily and successfully applied for population pharmacokinetics and therapeutic drug monitoring of GAB in patients with chronic pain. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:59:31Z 2021-06-25T10:59:31Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4322/2179-443X.0717 Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 42. 2179-443X 1808-4532 http://hdl.handle.net/11449/207701 10.4322/2179-443X.0717 2-s2.0-85105390895 |
url |
http://dx.doi.org/10.4322/2179-443X.0717 http://hdl.handle.net/11449/207701 |
identifier_str_mv |
Revista de Ciencias Farmaceuticas Basica e Aplicada, v. 42. 2179-443X 1808-4532 10.4322/2179-443X.0717 2-s2.0-85105390895 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista de Ciencias Farmaceuticas Basica e Aplicada |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128542043537408 |