Quantification of a COVID-19 Antibody Assay Using a Lateral Flow Test and a Cell Phone

Detalhes bibliográficos
Autor(a) principal: Thompson, Pearl
Data de Publicação: 2022
Outros Autores: Bovolato, Ana Lívia de Carvalho [UNESP], Ibáñez-Redín, Gisela, Brolo, Alexandre G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/chemosensors10070234
http://hdl.handle.net/11449/240364
Resumo: Although several biomedical assays have been developed to screen for antibodies against SARS-CoV-2, very few can be completed without drawing blood. We developed a rapid lateral flow screening tool that used saliva samples and yielded rapid results that could be quantified using a cell phone. This assay provided the sensitive detection of IgG antibodies against SARS-CoV-2 within 10 min. We started by synthesising, modifying, and characterising gold nanoparticles. Using these particles as a coloured label, we developed a lateral flow strip made of nitrocellulose, glass fibre, and cellulose material. We quantified our visual results using pictures acquired with a cell phone and calculated a limit of detection of 4 ng/mL of antibodies against the SARS-CoV-2 spike protein.
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spelling Quantification of a COVID-19 Antibody Assay Using a Lateral Flow Test and a Cell PhoneCOVID-19gold nanoparticlesIgGlateral flowsalivaSARS-CoV-2Although several biomedical assays have been developed to screen for antibodies against SARS-CoV-2, very few can be completed without drawing blood. We developed a rapid lateral flow screening tool that used saliva samples and yielded rapid results that could be quantified using a cell phone. This assay provided the sensitive detection of IgG antibodies against SARS-CoV-2 within 10 min. We started by synthesising, modifying, and characterising gold nanoparticles. Using these particles as a coloured label, we developed a lateral flow strip made of nitrocellulose, glass fibre, and cellulose material. We quantified our visual results using pictures acquired with a cell phone and calculated a limit of detection of 4 ng/mL of antibodies against the SARS-CoV-2 spike protein.Natural Sciences and Engineering Research Council of CanadaUniversity of VictoriaDepartment of Chemistry University of VictoriaInstitute of Biosciences São Paulo State University—UNESPSao Carlos Institute of Physics University of São Paulo—USPCentre for Advanced Materials and Related Technologies (CAMTEC) University of VictoriaInstitute of Biosciences São Paulo State University—UNESPUniversity of VictoriaUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Thompson, PearlBovolato, Ana Lívia de Carvalho [UNESP]Ibáñez-Redín, GiselaBrolo, Alexandre G.2023-03-01T20:13:52Z2023-03-01T20:13:52Z2022-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/chemosensors10070234Chemosensors, v. 10, n. 7, 2022.2227-9040http://hdl.handle.net/11449/24036410.3390/chemosensors100702342-s2.0-85133169547Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemosensorsinfo:eu-repo/semantics/openAccess2023-03-01T20:13:53Zoai:repositorio.unesp.br:11449/240364Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:57:02.650688Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Quantification of a COVID-19 Antibody Assay Using a Lateral Flow Test and a Cell Phone
title Quantification of a COVID-19 Antibody Assay Using a Lateral Flow Test and a Cell Phone
spellingShingle Quantification of a COVID-19 Antibody Assay Using a Lateral Flow Test and a Cell Phone
Thompson, Pearl
COVID-19
gold nanoparticles
IgG
lateral flow
saliva
SARS-CoV-2
title_short Quantification of a COVID-19 Antibody Assay Using a Lateral Flow Test and a Cell Phone
title_full Quantification of a COVID-19 Antibody Assay Using a Lateral Flow Test and a Cell Phone
title_fullStr Quantification of a COVID-19 Antibody Assay Using a Lateral Flow Test and a Cell Phone
title_full_unstemmed Quantification of a COVID-19 Antibody Assay Using a Lateral Flow Test and a Cell Phone
title_sort Quantification of a COVID-19 Antibody Assay Using a Lateral Flow Test and a Cell Phone
author Thompson, Pearl
author_facet Thompson, Pearl
Bovolato, Ana Lívia de Carvalho [UNESP]
Ibáñez-Redín, Gisela
Brolo, Alexandre G.
author_role author
author2 Bovolato, Ana Lívia de Carvalho [UNESP]
Ibáñez-Redín, Gisela
Brolo, Alexandre G.
author2_role author
author
author
dc.contributor.none.fl_str_mv University of Victoria
Universidade Estadual Paulista (UNESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Thompson, Pearl
Bovolato, Ana Lívia de Carvalho [UNESP]
Ibáñez-Redín, Gisela
Brolo, Alexandre G.
dc.subject.por.fl_str_mv COVID-19
gold nanoparticles
IgG
lateral flow
saliva
SARS-CoV-2
topic COVID-19
gold nanoparticles
IgG
lateral flow
saliva
SARS-CoV-2
description Although several biomedical assays have been developed to screen for antibodies against SARS-CoV-2, very few can be completed without drawing blood. We developed a rapid lateral flow screening tool that used saliva samples and yielded rapid results that could be quantified using a cell phone. This assay provided the sensitive detection of IgG antibodies against SARS-CoV-2 within 10 min. We started by synthesising, modifying, and characterising gold nanoparticles. Using these particles as a coloured label, we developed a lateral flow strip made of nitrocellulose, glass fibre, and cellulose material. We quantified our visual results using pictures acquired with a cell phone and calculated a limit of detection of 4 ng/mL of antibodies against the SARS-CoV-2 spike protein.
publishDate 2022
dc.date.none.fl_str_mv 2022-07-01
2023-03-01T20:13:52Z
2023-03-01T20:13:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/chemosensors10070234
Chemosensors, v. 10, n. 7, 2022.
2227-9040
http://hdl.handle.net/11449/240364
10.3390/chemosensors10070234
2-s2.0-85133169547
url http://dx.doi.org/10.3390/chemosensors10070234
http://hdl.handle.net/11449/240364
identifier_str_mv Chemosensors, v. 10, n. 7, 2022.
2227-9040
10.3390/chemosensors10070234
2-s2.0-85133169547
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Chemosensors
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128294352060416

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