Genetic and histopathological characterization of Toxoplasma gondii genotypes isolated from free-range chickens reared in the metropolitan region of Rio de Janeiro state, Brazil

Detalhes bibliográficos
Autor(a) principal: Casartelli-Alves, Luciana
Data de Publicação: 2021
Outros Autores: Pereira, Sandro Antonio, Ferreira, Luiz Claudio, Macedo Couto, Rodrigo de, Schubach, Tania Maria Pacheco, Amendoeira, Maria Regina Reis, Silva, Rodrigo Costa da, Langoni, Helio [UNESP], Millar, Patricia Riddell, Menezes, Rodrigo Caldas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00436-020-07011-9
http://hdl.handle.net/11449/210626
Resumo: This study aimed to genetically characterize Toxoplasma gondii isolates obtained from free-range chickens reared in the metropolitan region of the state of Rio de Janeiro, Brazil, and to evaluate the morbidity and histological changes associated with these isolates in mice. A mouse bioassay was used to isolate T. gondii from a pool of tissue samples (brain, heart, and thigh muscles) collected from 163 chickens. The 36 isolates obtained were genetically characterized by restriction fragment polymorphism (PCR-RFLP) analysis of the SAG1, 5 '-3 ' SAG2, aSAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico, and CS3 genomic regions. Seventeen atypical genotypes were identified and nine of them were reported for the first time. All identified genotypes caused clinical signs and histological changes in mice, with the majority being associated with high cumulative morbidity (65%) and severe or very severe histological changes (76%). The exclusive identification of atypical genotypes, with a predominance of new genotypes, indicates great genetic diversity of T. gondii in the region studied. In addition, the finding that all identified genotypes caused clinical signs and often severe histological changes in mice suggests potentially relevant virulence of these strains.
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spelling Genetic and histopathological characterization of Toxoplasma gondii genotypes isolated from free-range chickens reared in the metropolitan region of Rio de Janeiro state, BrazilBioassayChickensGenotypingHistopathologyToxoplasma gondiiMorbidityThis study aimed to genetically characterize Toxoplasma gondii isolates obtained from free-range chickens reared in the metropolitan region of the state of Rio de Janeiro, Brazil, and to evaluate the morbidity and histological changes associated with these isolates in mice. A mouse bioassay was used to isolate T. gondii from a pool of tissue samples (brain, heart, and thigh muscles) collected from 163 chickens. The 36 isolates obtained were genetically characterized by restriction fragment polymorphism (PCR-RFLP) analysis of the SAG1, 5 '-3 ' SAG2, aSAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico, and CS3 genomic regions. Seventeen atypical genotypes were identified and nine of them were reported for the first time. All identified genotypes caused clinical signs and histological changes in mice, with the majority being associated with high cumulative morbidity (65%) and severe or very severe histological changes (76%). The exclusive identification of atypical genotypes, with a predominance of new genotypes, indicates great genetic diversity of T. gondii in the region studied. In addition, the finding that all identified genotypes caused clinical signs and often severe histological changes in mice suggests potentially relevant virulence of these strains.Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundacao Oswaldo Cruz Fiocruz, Inst Nacl Infectol Evandro Chagas, Ave Brasil 4365, Rio De Janeiro, RJ, BrazilFundacao Oswaldo Cruz Fiocruz, Inst Oswaldo Cruz, Ave Brasil 4365, Rio De Janeiro, RJ, BrazilUniv Oeste Paulista, Fac Ciencias Agr, Presidente Prudente, SP, BrazilUniv Estadual Paulista, Fac Med Vet & Zootecnia, Botucatu, SP, BrazilUniv Fed Fluminense, Inst Biomed, Rua Prof Hernani Pires de Melo 101, Niteroi, RJ, BrazilUniv Estadual Paulista, Fac Med Vet & Zootecnia, Botucatu, SP, BrazilFAPERJ: APQ1 E-26/110.937/2009FAPERJ: APQ1 E-26/111.977/2012FAPERJ: TCT E-26/102.303/2011FAPERJ: JCNE E-26/102.247/2013CAPES: 001CNPq: MCT/CNPq 10/2010-AT-NMSpringerFundacao Oswaldo Cruz FiocruzUniv Oeste PaulistaUniversidade Estadual Paulista (Unesp)Universidade Federal Fluminense (UFF)Casartelli-Alves, LucianaPereira, Sandro AntonioFerreira, Luiz ClaudioMacedo Couto, Rodrigo deSchubach, Tania Maria PachecoAmendoeira, Maria Regina ReisSilva, Rodrigo Costa daLangoni, Helio [UNESP]Millar, Patricia RiddellMenezes, Rodrigo Caldas2021-06-25T23:35:56Z2021-06-25T23:35:56Z2021-01-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article665-677http://dx.doi.org/10.1007/s00436-020-07011-9Parasitology Research. New York: Springer, v. 120, n. 2, p. 665-677, 2021.0932-0113http://hdl.handle.net/11449/21062610.1007/s00436-020-07011-9WOS:000607178200016Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengParasitology Researchinfo:eu-repo/semantics/openAccess2021-10-23T22:04:48Zoai:repositorio.unesp.br:11449/210626Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T22:04:48Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Genetic and histopathological characterization of Toxoplasma gondii genotypes isolated from free-range chickens reared in the metropolitan region of Rio de Janeiro state, Brazil
title Genetic and histopathological characterization of Toxoplasma gondii genotypes isolated from free-range chickens reared in the metropolitan region of Rio de Janeiro state, Brazil
spellingShingle Genetic and histopathological characterization of Toxoplasma gondii genotypes isolated from free-range chickens reared in the metropolitan region of Rio de Janeiro state, Brazil
Casartelli-Alves, Luciana
Bioassay
Chickens
Genotyping
Histopathology
Toxoplasma gondii
Morbidity
title_short Genetic and histopathological characterization of Toxoplasma gondii genotypes isolated from free-range chickens reared in the metropolitan region of Rio de Janeiro state, Brazil
title_full Genetic and histopathological characterization of Toxoplasma gondii genotypes isolated from free-range chickens reared in the metropolitan region of Rio de Janeiro state, Brazil
title_fullStr Genetic and histopathological characterization of Toxoplasma gondii genotypes isolated from free-range chickens reared in the metropolitan region of Rio de Janeiro state, Brazil
title_full_unstemmed Genetic and histopathological characterization of Toxoplasma gondii genotypes isolated from free-range chickens reared in the metropolitan region of Rio de Janeiro state, Brazil
title_sort Genetic and histopathological characterization of Toxoplasma gondii genotypes isolated from free-range chickens reared in the metropolitan region of Rio de Janeiro state, Brazil
author Casartelli-Alves, Luciana
author_facet Casartelli-Alves, Luciana
Pereira, Sandro Antonio
Ferreira, Luiz Claudio
Macedo Couto, Rodrigo de
Schubach, Tania Maria Pacheco
Amendoeira, Maria Regina Reis
Silva, Rodrigo Costa da
Langoni, Helio [UNESP]
Millar, Patricia Riddell
Menezes, Rodrigo Caldas
author_role author
author2 Pereira, Sandro Antonio
Ferreira, Luiz Claudio
Macedo Couto, Rodrigo de
Schubach, Tania Maria Pacheco
Amendoeira, Maria Regina Reis
Silva, Rodrigo Costa da
Langoni, Helio [UNESP]
Millar, Patricia Riddell
Menezes, Rodrigo Caldas
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fundacao Oswaldo Cruz Fiocruz
Univ Oeste Paulista
Universidade Estadual Paulista (Unesp)
Universidade Federal Fluminense (UFF)
dc.contributor.author.fl_str_mv Casartelli-Alves, Luciana
Pereira, Sandro Antonio
Ferreira, Luiz Claudio
Macedo Couto, Rodrigo de
Schubach, Tania Maria Pacheco
Amendoeira, Maria Regina Reis
Silva, Rodrigo Costa da
Langoni, Helio [UNESP]
Millar, Patricia Riddell
Menezes, Rodrigo Caldas
dc.subject.por.fl_str_mv Bioassay
Chickens
Genotyping
Histopathology
Toxoplasma gondii
Morbidity
topic Bioassay
Chickens
Genotyping
Histopathology
Toxoplasma gondii
Morbidity
description This study aimed to genetically characterize Toxoplasma gondii isolates obtained from free-range chickens reared in the metropolitan region of the state of Rio de Janeiro, Brazil, and to evaluate the morbidity and histological changes associated with these isolates in mice. A mouse bioassay was used to isolate T. gondii from a pool of tissue samples (brain, heart, and thigh muscles) collected from 163 chickens. The 36 isolates obtained were genetically characterized by restriction fragment polymorphism (PCR-RFLP) analysis of the SAG1, 5 '-3 ' SAG2, aSAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, Apico, and CS3 genomic regions. Seventeen atypical genotypes were identified and nine of them were reported for the first time. All identified genotypes caused clinical signs and histological changes in mice, with the majority being associated with high cumulative morbidity (65%) and severe or very severe histological changes (76%). The exclusive identification of atypical genotypes, with a predominance of new genotypes, indicates great genetic diversity of T. gondii in the region studied. In addition, the finding that all identified genotypes caused clinical signs and often severe histological changes in mice suggests potentially relevant virulence of these strains.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T23:35:56Z
2021-06-25T23:35:56Z
2021-01-08
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00436-020-07011-9
Parasitology Research. New York: Springer, v. 120, n. 2, p. 665-677, 2021.
0932-0113
http://hdl.handle.net/11449/210626
10.1007/s00436-020-07011-9
WOS:000607178200016
url http://dx.doi.org/10.1007/s00436-020-07011-9
http://hdl.handle.net/11449/210626
identifier_str_mv Parasitology Research. New York: Springer, v. 120, n. 2, p. 665-677, 2021.
0932-0113
10.1007/s00436-020-07011-9
WOS:000607178200016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Parasitology Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 665-677
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799965382256099328

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